产品编号 A102398 | CAS号 38966-21-1 | Aladdin
货号 规格 库存 价格 数量
A102398-10mg 98% 上海(货期不确定)
A102398-1mg 98% 上海(货期不确定)
A102398-5mg 98% 上海(货期不确定)


敏感性 对光敏感
密度 1.2200
溶解性 Soluble in ethanol (1 mg/ml,stable at least a week at 4°C.), DMSO (10 mg/ml,stable at least six weeks at -20°C.), and methanol (10 mg/ml). Insoluble in water.
存贮条件 储存温度2-8°C


产品介绍 Aphidicolin is a tetracyclic diterpene antibiotic and DNA synthesis inhibitor isolated from Cephalosporium aphidicola. Aphidicolin is reported to specifically inhibit DNA polymerase α and δ via binding to the enzyme, in eukaryotic cells such as the HeLa cell line, without affecting other DNA polymerases. This compound blocks the cell cycle at early S phase and has been reported to be useful for synchronizing cell cycles in cultured eukaryotic cells. This compound is also noted to induce apoptosis in HeLa S3 cells after 18-24 hours of the cell cycle.
别名 Aphidicolin;APC
应用 A novel tetracyclic diterpene antibiotic and also a DNA synthesis inhibitor in eukaryotes
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安全声明(欧洲) 22,24
个人防护设备 Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter
RTECS PB9185000
WGK德国 3
Akira Kikuchi, Masashi Asahina, Motoki Tanaka, Shinobu Satoh, Hiroshi Kamada
Totipotency is the ability of a cell to regenerate the entire organism, even after previous differentiation as a specific cell. When totipotency is coupled with active cell division, it was presumed t...Read More
S S Spadari, F F Focher, F F Sala, G G Ciarrocchi, G G Koch, A A Falaschi, G G Pedrali-Noy
Aphidicolin, a tetracyclic diterpenoid obtained from the culture filtrates of Cephalosporium aphidicola and other fungi, inhibits the growth of eukaryotic cells and of certain animal viruses (SV40, He...Read More
K K Yamada, R R Itoh
DNA polymerases involved in ultraviolet (UV)-induced DNA repair were studied in human fibroblasts using the inhibitors of DNA polymerases, aphidicolin which inhibits DNA polymerases alpha, delta and e...Read More
M C MC Mutomba, C C CC Wang
The effects of aphidicolin (APH) and hydroxyurea (HU) on the cell cycle and differentiation of Trypanosoma brucei bloodstream forms were studied. APH (0.1 microgram ml-1) inhibited cell division, but ...Read More
K Kuwakado, M Kubota, R Bessho, A Kataoka, I Usami, Y W Lin, A Okuda, Y Wakazono
1-beta-D-arabinofuranosylcytosine (ara-C) (2 microM) can induce apoptosis in a human myeloid leukemia cell line, U937, after 4 h of incubation. Pretreatment of cells with aphidicolin (2 microM) augmen...Read More
N D Wang, J R Testa, D I Smith
The constitutive 3p14.2 fragile site is the most highly inducible fragile site in the human genome. This locus may predispose chromosome 3 to specific losses due to deletions and translocations that h...Read More
B F BF McAllister, I F IF Greenbaum
Aphidicolin (APC)-induced chromosomal gaps and breaks were analyzed for ten deer mice (Peromyscus maniculatus) from a natural population. The FSM statistical methodology was used to identify fragile s...Read More
Michael J Humphries, Kirsten H Limesand, Jonathan C Schneider, Keiichi I Nakayama, Steven M Anderson, Mary E Reyland
Protein kinase C (PKC) delta is an essential regulator of mitochondrial dependent apoptosis in epithelial cells. We have used the PKCdelta(-/-) mouse to ask if loss of PKCdelta protects salivary gland...Read More
Christian E CE Isaac, Sarah M SM Francis, Alison L AL Martens, Lisa M LM Julian, Laurie A LA Seifried, Natalie N Erdmann, Ulrich K UK Binné, Lea L Harrington, Piotr P Sicinski, Nathalie G NG Bérubé, Nicholas J NJ Dyson, Frederick A FA Dick
The retinoblastoma protein (pRb) has been proposed to regulate cell cycle progression in part through its ability to interact with enzymes that modify histone tails and create a repressed chromatin st...Read More
Jenny J Rowe, Rebecca J RJ Greenblatt, Dongmei D Liu, Jennifer F JF Moffat
Varicella-zoster virus (VZV) replicates in quiescent T cells, neurons, and skin cells. In cultured fibroblasts (HFFs), VZV induces host cyclin expression and cyclin-dependent kinase (CDK) activity wit...Read More
Jan-Willem JW Taanman, Margit M Heiske, Thierry T Letellier
Synthesis of mitochondrial DNA is performed by DNA polymerase @c. Mutations in POLG, the gene encoding the catalytic subunit of DNA polymerase @c, are a major cause of neurological disease. A large pr...Read More
Tomoko Funakoshi, Michaela Clever, Ai Watanabe, Naoko Imamoto
The nuclear pore complex (NPC) is a large protein assembly that mediates molecular trafficking between the cytoplasm and the nucleus. NPCs assemble twice during the cell cycle in metazoans: postmitosi...Read More
Nathalie Franck, Emilie Montembault, Pierre Romé, Aude Pascal, Jean-Yves Cremet, Régis Giet
CDK11(p58) is a mitotic protein kinase, which has been shown to be required for different mitotic events such as centrosome maturation, chromatid cohesion and cytokinesis.In addition to these previous...Read More
Nicola Crosetto, Abhishek Mitra, Maria Joao Silva, Magda Bienko, Norbert Dojer, Qi Wang, Elif Karaca, Roberto Chiarle, Magdalena Skrzypczak, Krzysztof Ginalski, Philippe Pasero, Maga Rowicka, Ivan Dikic
We present a genome-wide approach to map DNA double-strand breaks (DSBs) at nucleotide resolution by a method we termed BLESS (direct in situ breaks labeling, enrichment on streptavidin and next-gener...Read More
Günter Speit, Christine Leibiger, Stefanie Kuehner, Josef H?gel
The comet assay is increasingly used to measure the repair of various types of DNA damage. Modifications of the standard protocol have been introduced to determine the repair capacity of specific DNA ...Read More
Seiki Nakao, Sufang Zhang, Markku Vaara, Juhani E Syv?oja, Marietta Y Lee, Toshiki Tsurimoto, Peter Karran, Shinya Oda
In?vitro assay of mammalian DNA replication has been variously approached. Using gapped circular duplex substrates containing a 500-base single-stranded DNA region, we have constructed a mammalian cel...Read More
Alba Llopis, Noelia Salvador, Amaia Ercilla, Sandra Guaita-Esteruelas, Ivan Barrantes, Jalaj Gupta, Matthias Gaestel, Roger Davis, Angel Nebreda, Neus Agell
Accurate DNA replication is crucial for the maintenance of genome integrity. To this aim, cells have evolved complex surveillance mechanisms to prevent mitotic entry in the presence of partially repli...Read More
T Katherine TK Tamai, Lucy C LC Young, Catherine A CA Cox, David D Whitmore
A fundamental role of the circadian clock is to control biochemical and physiological processes such that they occur an optimal time of day. One of the most significant clock outputs from a clinical a...Read More
Jay Yang, Lara O'Donnell, Daniel Durocher, Grant W Brown
RMI1 is a member of an evolutionarily conserved complex composed of BLM and topoisomerase IIIα (TopoIIIα). This complex exhibits strand passage activity in vitro, which is likely important for DNA r...Read More
Min Huang, Hui Li, Lu Zhang, Fei Gao, Pu Wang, Yong Hu, Shihan Yan, Lin Zhao, Qi Zhang, Junjun Tan, Xincheng Liu, Shibin He, Lijia Li
Our previous study demonstrated that 45S ribosomal DNA (45S rDNA) clusters were chromosome fragile sites expressed spontaneously in Lolium. In this study, fragile phenotypes of 45S rDNA were observed ...Read More
Rachid Amsailale, Eric Van Den Neste, Angélique Arts, Eliza Starczewska, Fran?oise Bontemps, Caroline Smal
Deoxycytidine kinase (dCK) (EC is a key enzyme in the activation of several therapeutic nucleoside analogs (NA). Its activity can be increased in vivo by Ser-74 phosphorylation, a property t...Read More
Arkarachai Fungtammasan, Erin Walsh, Francesca Chiaromonte, Kristin A Eckert, Kateryna D Makova
Chromosomal common fragile sites (CFSs) are unstable genomic regions that break under replication stress and are involved in structural variation. They frequently are sites of chromosomal rearrangemen...Read More
Martin F Arlt, Thomas E Wilson, Thomas W Glover
Copy number variants (CNVs) are widely distributed throughout the human genome, where they contribute to genetic variation and phenotypic diversity. De novo CNVs are also a major cause of numerous gen...Read More
Ting-Yu TY Zhang, Jian-Jun JJ Dai, Cai-Feng CF Wu, Xiao-Long XL Gu, Liang L Liu, Zhi-Qiang ZQ Wu, Yi-Ni YN Xie, Bin B Wu, Hui-Lan HL Chen, Yao Y Li, Xue-Jin XJ Chen, De-Fu DF Zhang
To optimize somatic cell nuclear transfer (SCNT) procedures in mini-pigs, the present study was designed to examine the effects of donor cell types and aphidicolin (APC) treatment on in vitro developm...Read More
Avik K Ghosh, Marie L Rossi, Dharmendra Kumar Singh, Christopher Dunn, Mahesh Ramamoorthy, Deborah L Croteau, Yie Liu, Vilhelm A Bohr
Telomeres are structures at the ends of chromosomes and are composed of long tracks of short tandem repeat DNA sequences bound by a unique set of proteins (shelterin). Telomeric DNA is believed to for...Read More
Kenjiro Asagoshi, Wade Lehmann, Elena K Braithwaite, Lucas Santana-Santos, Rajendra Prasad, Jonathan H Freedman, Bennett Van Houten, Samuel H Wilson
The base excision DNA repair (BER) pathway known to occur in Caenorhabditis elegans has not been well characterized. Even less is known about the DNA polymerase (pol) requirement for the gap-filling s...Read More
Ryuya R Fujii, Atsushi A Minami, Tae T Tsukagoshi, Natsuko N Sato, Takehiko T Sahara, Satoru S Ohgiya, Katsuya K Gomi, Hideaki H Oikawa
Clustering of biosynthetic genes for producing fungal secondary metabolites, which frequently consist of less than ten genes, has been recognized with numerous genomes. The heterologous expression of ...Read More
Terri G Edwards, Michael J Helmus, Kevin Koeller, James K Bashkin, Chris Fisher
A highly reproducible quantitative PCR (Q-PCR) assay was used to study the stability of human papillomavirus (HPV) in undifferentiated keratinocytes that maintain viral episomes. The term "stabil...Read More
Wen Deng, Sai Wah Tsao, Xin-Yuan Guan, Annie L M Cheung
Chromosomal instability is the major form of genomic instability in cancer cells. Amongst various forms of chromosomal instability, pericentromeric or centromeric instability remains particularly poor...Read More
Martin F Arlt, Sountharia Rajendran, Shanda R Birkeland, Thomas E Wilson, Thomas W Glover
Spontaneous copy number variant (CNV) mutations are an important factor in genomic structural variation, genomic disorders, and cancer. A major class of CNVs, termed nonrecurrent CNVs, is thought to a...Read More
Stephan Heermann, Bj?rn Spittau, Katalin Zajzon, Markus H Schwab, Kerstin Krieglstein
During development neural crest derived Schwann Cell (SC) precursors migrate to nerve trunks and populate nascent nerves. Axonal ensheathment by SC is a prerequisite for normal nerve function and the ...Read More
Artem K Velichko, Nadezhda V Petrova, Omar L Kantidze, Sergey V Razin
Heat shock (HS) is one of the better-studied exogenous stress factors. However, little is known about its effects on DNA integrity and the DNA replication process. In this study, we show that in G1 an...Read More
Martin F Arlt, Alev Cagla Ozdemir, Shanda R Birkeland, Thomas E Wilson, Thomas W Glover
Copy number variants (CNVs) are widely distributed throughout the human genome, where they contribute to genetic variation and phenotypic diversity. Spontaneous CNVs are also a major cause of genetic ...Read More
Laetitia Finzi, Aurore Kraemer, Claude Capron, Severine Noullet, Diane Goere, Christophe Penna, Bernard Nordlinger, Josette Legagneux, Jean-Fan?ois Emile, Robert Malafosse
Cancer gene therapy by retroviral vectors is mainly limited by the level of transduction. Retroviral gene transfer requires target cell division. Cell synchronization, obtained by drugs inducing a rev...Read More
Anne A Letessier, Daniel D Birnbaum, Michelle M Debatisse, Max M Chaffanet
We're sorry this abstract is currently not available....Read More
Rafael Bernad, Patricia Sánchez, Teresa Rivera, Miriam Rodríguez-Corsino, Ekaterina Boyarchuk, Isabelle Vassias, Dominique Ray-Gallet, Alexei Arnaoutov, Mary Dasso, Geneviève Almouzni, Ana Losada
Centromeric protein A (CENP-A) is the epigenetic mark of centromeres. CENP-A replenishment is necessary in each cell cycle to compensate for the dilution associated to DNA replication, but how this is...Read More
Aline V Probst, Ikuhiro Okamoto, Miguel Casanova, Fatima El Marjou, Patricia Le Baccon, Geneviève Almouzni
At the time of fertilization, the paternal genome lacks the typical configuration and marks characteristic of pericentric heterochromatin. It is thus essential to understand the dynamics of this regio...Read More
Krishna P Kota, Jacqueline G Benko, Rajini Mudhasani, Cary Retterer, Julie P Tran, Sina Bavari, Rekha G Panchal
Viruses modulate a number of host biological responses including the cell cycle to favor their replication. In this study, we developed a high-content imaging (HCI) assay to measure DNA content and id...Read More