|产品介绍||Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.|
|应用||Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.|
|个人防护设备||Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter|
The crystal structure of avidin, a protein from hen egg white, was determined in the form of a complex with biotinylruthenocene. This biotin-derived organometallic ligand is a potential anticancer age...Read More
Ligation of major histocompatability complex (MHC) class I molecules on human T cells induces cell death through PI-3 kinase-induced c-Jun NH2-terminal kinase activity: a novel apoptotic pathway distinct from Fas-induced apoptosis.
Ligation of major histocompatability complex class I (MHC-I) molecules expressed on T cells leads to both growth arrest and apoptosis. The aim of the current study was to investigate the intracellular...Read More
The perifollicular and marginal zones of the human splenic white pulp : do fibroblasts guide lymphocyte immigration?
We investigate the white pulp compartments of 73 human spleens and demonstrate that there are several microanatomical peculiarities in man that do not occur in rats or mice. Humans lack a marginal sin...Read More
Cultured skin substitutes consisting of implantable collagen (COL) and cultured human skin cells often fail clinically from destruction by microbial contamination. Hypothetically, addition of selected...Read More
Label-free impedimetric immunosensors for psoriasin--increased reproducibility and sensitivity using an automated dispensing system.
We describe within this paper the construction of a label-free immunosensor for the protein psoriasin (S100A7), which is associated with a number of clinical conditions such as skin diseases or cancer...Read More
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We have investigated the effects of cross-linking perturbations on lipid phase-domain coalescence. Our model system explores cross-linking in the fluid-disordered phase of two-phase vesicles. Here, we...Read More
Described herein is a unique and inexpensive method that outperforms commercial methods that amplify the streptavidin-biotin recognition event. Amplification induced by streptavidin and biotinylated p...Read More
We describe a new class of fluorescent reporter and its employment to visualize the biotin/avidin binding interaction. Derivatives of the azamacrocycle cyclam that contain a pendant naphthalimide dye ...Read More
Capillary electrophoresis (CE) methods using proteins as the chiral selectors have been developed for the separation of enantiomeric mixtures. For chiral separations in protein-based CE, two methods w...Read More
About 40% of the hotspots for meiotic recombination contain the degenerate consensus sequence 5'-CCNCCNTNNCCNC-3'. Here we present a novel protocol for enriching hotspot sequences from diges...Read More
Block copolymer modified surfaces for conjugation of biomacromolecules with control of quantity and activity.
Polymer brush layers based on block copolymers of poly(oligo(ethylene glycol) methacrylate) (POEGMA) and poly(glycidyl methacrylate) (PGMA) were formed on silicon wafers by activators generated by ele...Read More
Development of value-added micropatterned porous materials from naturally abundant polymers, such as cellulose, are of growing interest. In this paper, regioselectively modified amphiphilic cellulose ...Read More
Abstract Purpose: Pretargeted radioimmunotherapy (PRIT) against intraperitoneal (i.p.) ovarian microtumors using avidin-conjugated monoclonal antibody MX35 (avidin-MX35) and (211)At-labeled, biotinyla...Read More
Measurement of local permeability at subcellular level in cell models of agonist- and ventilator-induced lung injury.
Alterations of cell monolayer integrity and increased vascular permeability are key to many pathologies, including atherosclerosis, stroke, lung injury, cancer, digestive disorders and others. Current...Read More
A new construction of a test-system for free thyroid hormone 3,3',5-triiodothyronine determination (free T3) in human blood serum in ELISA and IRMA variants was described. For this purpose a low-...Read More
Mimicking the biological ligand-receptor principle for universal target gene delivery mediated by avidin-biotin interaction.
A facile and flexible targeting gene-delivery strategy is reported by mimicking the biological ligand-receptor principle based on avidin-biotin interaction. In vitro and in vivo investigations demonst...Read More
Preparation of hyaluronic Acid micro-hydrogel by biotin-avidin-specific bonding for Doxorubicin-targeted delivery.
Hyaluronic acid is a naturally ionic polysaccharide with cancer cell selectivity. It is an ideal candidate material for delivery of anticancer agents. In this study, hyaluronic acid (HA) micro-hydroge...Read More
The precise accumulation of protein functions on a nanoscale to fabricate advanced biomaterials has become possible by a bottom-up approach based on molecular self-assembly. The avidin-biotin interact...Read More
The screening of ligands against proteins plays important role in drug discovery and biological research. Using a dye labelled Streptavidin binding aptamer (SBA) as a competitive reporter probe, we fo...Read More
The avidin-biotin system is a highly specific reaction that has been used in a wide range of biomedical applications, including surface modification and cell patterning. We systematically examined a n...Read More
Microbubbles (MBs) are increasingly being proposed as delivery vehicles for targeted therapeutics, as well as being contrast agents for ultrasound imaging. MBs formed with a lipid shell are promising ...Read More
In this study, we investigated the effect of secondary Bjerknes forces on targeted microbubbles using high-speed optical imaging. We observed that targeted microbubbles attached to an underlying surfa...Read More
Fibril formation by mutational variants of human lysozyme is associated with a fatal form of hereditary non-neuropathic systemic amyloidosis. Defining the mechanistic details of lysozyme aggregation i...Read More
This work investigates self-assembled monolayers that were formed from a glycol-terminated thiol monomer and were patterned using photoinduced monolayer desorption. Utilizing direct-write photolithogr...Read More
Highly sensitive fluorescence detection of avidin/streptavidin with an optical interference mirror slide.
This paper presents highly sensitive fluorescence detections of avidin and streptavidin using an optical interference mirror (OIM) slide consisting of a plane mirror covered with an optical interferen...Read More
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Micro-emulsion synthesis, surface modification, and photophysical properties of Zn(1-x) Mn(x)S nanocrystals for biomolecular recognition.
In this research, we mainly focused on the micro-emulsion synthesis of biotinylated ZnS (zinc sulfide) nanocrystals for avidin recognition. Various samples of Zn(1-x)Mn(x) S, with x = 0.0001, 0.007, 0...Read More