|别名||甲基氰;氰甲烷;乙烷腈;色谱乙腈;ACN;Methy cyanide; Cyanomethane; Ethanenitrile; ACN|
|个人防护设备||Eyeshields,Faceshields,full-face respirator (US),Gloves,multi-purpose combination respirator cartridge (US),type ABEK (EN14387) respirator filter|
While tailing and overloading of basic compounds remain problematic on most RP columns, a new kind of positively charged RP column named XCharge C18 was found to be superior good for the separation of...Read More
High throughput proteomic analysis of the secretome in an explant model of articular cartilage inflammation.
This study employed a targeted high-throughput proteomic approach to identify the major proteins present in the secretome of articular cartilage. Explants from equine metacarpophalangeal joints were i...Read More
Glycerol nucleic acid (GNA) has an acyclic phosphoglycerol backbone repeat-unit, but forms stable duplexes based on Watson-Crick base-pairing. Because of its structural simplicity, GNA is of particula...Read More
Polyphosphates and pyrophosphates of hexopyranoses as allosteric effectors of human hemoglobin: synthesis, molecular recognition, and effect on oxygen release.
Polyphosphorylated and perphosphorylated hexopyranose monosaccharides and disaccharides were synthesized from parent or partially protected carbohydrates as potential allosteric effectors of hemoglobi...Read More
Photoluminescence and thermoanalytical studies of complexes based on 5-Cl-8-hydroxyquinoline and calixarene ligands.
A innovative 5-Cl-8-oxyquinolinepropoxycalixarene ligand (2) have been prepared, exhibiting, at room temperature, blue fluorescent light emission and resulting in shift band to green fluorescent li...Read More
Adaptation of dynamic covalent systems of imine constituents to medium change by component redistribution under reversible phase separation.
A dynamic covalent library of interconverting imine constituents, dissolved in an acetonitrile/water mixture, undergoes constitutional reorganization upon phase separation induced by a physical stimul...Read More
Convenient QSAR model for predicting the complexation of structurally diverse compounds with @b-cyclodextrins
This paper reports a QSAR study for predicting the complexation of a large and heterogeneous variety of substances (233 organic compounds) with @b-cyclodextrins (@b-CDs). Several different theoretical...Read More
Ultra high performance liquid chromatography/tandem mass spectrometry based identification of steroid esters in serum and plasma: an efficient strategy to detect natural steroids abuse in breeding and racing animals.
During last decades, the use of natural steroids in racing and food producing animals for doping purposes has been flourishing. The endogenous or exogenous origin of these naturally occurring steroids...Read More
Nitric oxide reactivity of Cu(II) complexes of tetra- and pentadentate ligands: structural influence in deciding the reduction pathway.
Four Cu(II) complexes, 1, 2, 3 and 4, are synthesized with ligands, L1, L2, L3 and L4 [L1 = N1,N2-bis((pyridin-2-yl)methyl)ethane-1,2-diamine; L2 = N1,N3-bis((pyridin-2-yl)methyl)propane-1,3-diamine; ...Read More
Distribution of pesticides in n-hexane/water and n-hexane/acetonitrile systems and estimation of possibilities of their extraction isolation and preconcentration from various matrices.
Distribution of 150 most widely used pesticides of different chemical classes (amides, anilinopirimidines, aromatics, benzenesulfonates, carbamates, dicarboximides, organophosphorus compounds, phenyl ...Read More
Influence of dTMP on the phenotypic appearance and intracellular persistence of Staphylococcus aureus.
Thymidine-dependent small-colony variants (SCVs) of Staphylococcus aureus are frequently associated with persistent and recurrent infections in cystic fibrosis patients. The phenotypic appearance of S...Read More
Mixed-mode chromatographic materials are more and more often used for the purification of biomolecules, such as peptides and proteins. In many instances they in fact exhibit better selectivity values ...Read More
The study was aimed at developing a simple HPLC method for the determination of the content of impurities in Gynalgin, a two-component preparation. A satisfactory separation was performed on 250 x 4.6...Read More
The neurotoxic non-protein amino acid, β-N-methylamino-L-alanine (BMAA), was first associated with the high incidence of Amyotrophic Lateral Sclerosis/Parkinsonism Dementia Complex (ALS/PDC) in Guam....Read More
The hydrogen-bonded dianion of vitamin k1 produced in aqueous-organic solutions exists in equilibrium with its hydrogen-bonded semiquinone anion radical.
When the quinone, vitamin K (VK), is electrochemically reduced in aqueous-acetonitrile solutions (CHCN with 7.22 M HO), it undergoes a two-electron reduction to form the dianion that is hydrogen-bonde...Read More
In-capillary determination of creatinine with electrophoretically mediated microanalysis: characterization of the effects of reagent zone and buffer conditions.
Previous work has demonstrated proof-of-concept for carrying out the clinically useful Jaffe reaction between creatinine and picrate within a capillary tube using electrophoretically mediated microana...Read More
Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP-MS) yielded a wealth of information on...Read More
Direct bacterial profiling by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry for identification of pathogenic Neisseria.
The present study investigates the suitability of direct bacterial profiling as a tool for the identification and subtyping of pathogenic Neisseria. The genus Neisseria includes two human pathogens, N...Read More
The title compound, [Na(C(2)BF(2)O(4))(CH(3)CN)(2)](n), forms infinite two-dimensional layers running parallel to (010). The layers lie across crystallographic mirror planes at y = 1/4 and 3/4. The Na...Read More
Commercially available protein reference standard materials are widely used for the quantitation of intact proteins in biopharmaceuticals, food, and consumer products. However, the purity of protein r...Read More
Protein isoaspartate methyltransferase prevents apoptosis induced by oxidative stress in endothelial cells: role of Bcl-Xl deamidation and methylation.
Natural proteins undergo in vivo spontaneous post-biosynthetic deamidation of specific asparagine residues with isoaspartyl formation. Deamidated-isomerized molecules are both structurally and functio...Read More
Solvent-dependent molecular structure of ionic species directly measured by ultrafast x-ray solution scattering.
Ionic species often play important roles in chemical reactions occurring in water and other solvents, but it has been elusive to determine the solvent-dependent molecular structure with atomic resolut...Read More
Determination of zolpidem in human hair by micropulverized extraction based on the evaluation of relative extraction efficiency of seven psychoactive drugs from an incurred human hair specimen.
A micropulverization method for rapid extraction of psychoactive drugs from hair was developed. A hair sample (10mg) was micropulverized for 10min at 42Hz with 0.2mL of 45% (w/v) aqueous ammonium phos...Read More
Detection and identification of hydrolysis products of sulfur mustards at trace levels in environmental samples using liquid chromatography solid phase extraction combined with off-line nuclear magnetic resonance analysis.
An LC-UV-SPE NMR method for the analysis of polar hydrolysis products of the chemical warfare agents known as sulfur mustards at low ppm levels in environmental samples is developed. The hydrolysis pr...Read More
The use of biopartitioning micellar chromatography and immobilized artificial membrane column for in silico and in vitro determination of blood-brain barrier penetration of phenols.
Biopartitioning Micellar Chromatography (BMC) is a mode of micellar liquid chromatography that uses C18 stationary phases and micellar mobile phases of Brij35 under adequate experimental conditions an...Read More
Amine modified graphene as reversed-dispersive solid phase extraction materials combined with liquid chromatography-tandem mass spectrometry for pesticide multi-residue analysis in oil crops.
Amine modified graphene is successfully synthesized via a one-pot solvothermal reaction between graphene oxide and ammonia water, methylamine or n-butyl amine. The presence of amine groups in graphene...Read More
A liquid chromatography-mass spectrometric method was developed and validated to determine six polyether ionophores (lasalocid sodium, monensin sodium, salinomycin sodium, narasin, maduramicin ammoniu...Read More
One of the barriers to the development of protein therapeutics is effective delivery to mammalian cells. The proteins must maintain a careful balance of polar moieties to enable administration and dis...Read More
Approaches to model the retention and peak profile in linear gradient reversed-phase liquid chromatography.
The optimisation of the experimental conditions in gradient reversed-phase liquid chromatography requires reliable algorithms for the description of the retention and peak profile. As in isocratic elu...Read More
An autocatalytic radical chain pathway in formation of an iron(iv)-oxo complex by oxidation of an iron(ii) complex with dioxygen and isopropanol.
Evidence of an autocatalytic radical chain pathway has been reported in formation of a non-heme iron(iv)-oxo complex by oxidation of an iron(ii) complex with dioxygen and isopropanol in acetonitrile a...Read More
Effect of the pH and the ionic strength on overloaded band profiles of weak bases onto neutral and charged surface hybrid stationary phases in reversed-phase liquid chromatography.
This work reports on the effects of the solution pH and its ionic strength on the overloaded band profiles and the parameters of the adsorption isotherms of nortriptylinium hydrochloride on the bridge...Read More
Design of experiments and multivariate analysis for evaluation of reversed-phase high-performance liquid chromatography with charged aerosol detection of sucrose caprate regioisomers.
The use of step-down gradient elution profiles to improve separation of sucrose caprate regioisomers was investigated as part of the development of a quantitative RP-HPLC analysis method with charged ...Read More
Characterization and quantification of racemic and meso-ethylenediamine-N,N'-bis(2-hydroxy-5-sulfophenylacetic) acid/iron (III) by ion-pair ultra-high performance liquid chromatography coupled with diode array detector and electrospray tandem mass sp
EDDHSA/Fe is a promising substitute of EDDHA/Fe to fight iron chlorosis. o,o-EDDHSA structure contains two chiral carbons giving the racemic and meso couples of stereoisomers. Ion-pair HPLC and UHPLC-...Read More
Theoretical Studies for Large Tunneling and the Hydrogen-Transfer Mechanism in the C?H Activation of CH3 CN by a Di(μ-oxo)diiron(IV) Complex: A Model for Intermediate Q in Soluble Methane Monooxygenase.
Tracking the transfer: The potential-energy surface along the intrinsic reaction coordinate was constructed to study the detailed mechanism of hydrogen transfer in the C?H activation of CH CN by a di(...Read More
Reduction and Protonation of Mo(IV) Imido Complexes with depe Coligands: Generation and Reactivity of a S = 1/2 Mo(III) Alkylnitrene Intermediate.
Reduction and protonation of Mo(IV) imido complexes with diphosphine coligands constitutes the second part of the Chatt cycle for biomimetic reduction of N to ammonia. In order to obtain insights into...Read More
Determination of linezolid in human serum by reversed-phase high-performance liquid chromatography with ultraviolet and diode array detection.
A high-performance liquid chromatographic (HPLC) method with UV and DAD detection for the quantitative determination of linezolid in human serum was developed in present work. Chromatography was carri...Read More
The SPE-HPLC method was developed to determine an isosalipurposide (5) and its derivative, 6"-O-p-coumaroyl ester (6) in the bark of eight taxa (I-VIII) belonging to three species of the genus Sa...Read More
Preparation and characterization of open-tubular capillary column modified with graphene oxide nanosheets for the separation of small organic molecules.
A novel coated capillary was prepared by immobilizing graphene oxide (GO) on the fused-silica capillary (75 μm i.d.) which was derivatized by 3-aminopropyl-trimethoxysilane (APTMS). The bare capillar...Read More
In this work we present a general procedure for the model-based optimization of a polypeptide crude mixture purification process through its application to a case of industrial relevance. This is done...Read More
Effect of the ionic strength on the adsorption process of an ionic surfactant onto a C18-bonded charged surface hybrid stationary phase at low pH.
The adsorption behavior of nortriptylinium hydrochloride was investigated in detail on a new C-bonded charged surface hybrid stationary phase (CSH-C, Waters) at a constant pH of 2.60 (phosphate buffer...Read More
We present a simple approach for the calculation of accurate pKa values in water and acetonitrile based on the straightforward calculation of the gas-phase absolute free energies of the acid and conju...Read More
Direct evidence of 2-cyano-2-propoxy radical activity during AIBN-based oxidative stress testing in acetonitrile-water solvent systems.
Oxidative susceptibility testing was performed on a drug substance containing a methoxy-naphthalene moiety. 2,2'-azobisisobutyronitrile (AIBN) was employed to initiate peroxy radical oxidation to...Read More
To establish the method for measurement of acetonitrile in blood and urine by head-space gas chromatography.DB-ALC1 (30 m x 320 microm x 1.8 microm) and DB-ALC2 (30 m x 320 microm x 1.2 microm) capill...Read More
A method for the simultaneous determination of multiple pesticide residues in agricultural products was developed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The sample ...Read More
myo-Inositol trispyrophosphate: a novel allosteric effector of hemoglobin with high permeation selectivity across the red blood cell plasma membrane.
myo-Inositol trispyrophosphate (ITPP), a novel membrane-permeant allosteric effector of hemoglobin (Hb), enhances the regulated oxygen release capacity of red blood cells, thus counteracting the effec...Read More
Malignant catarrhal fever of cattle is associated with low abundance of IL-2 transcript and a predominantly latent profile of ovine herpesvirus 2 gene expression.
Malignant catarrhal fever (MCF) is a lethal disease of cattle, characterized by vasculitis, necrosis, and accumulation of activated, dysregulated cytotoxic lymphocytes in various tissues. Ovine gamma ...Read More