|产品介绍||AEC, 50X (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Although not as sensitive as diaminobenzidine (DAB, 50X;, the red deposit formed at the sites of AEC oxidation make this substrate useful for double labeling. Aqueous-based mounting medium and counterstains must be used for IHC applications since oxidized AEC is soluble in alcohols and xylene (e.g., Crystal Mounting Medium; ). Use with AEC Substrate Buffer .|
|别名||AEC, 50X ;3-氨基-N-乙基咔唑;AEC, 50X|
? Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps.
? Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X;).
? Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well.
? Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT.
? Rinse thoroughly with distilled water. If desired, follow with an appropriate aqueous-based counterstain.
? Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; )
? Unused AEC solution can be discarded according to city, county, state, province or country’s regulations
|个人防护设备||Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges|
[High performance liquid chromatography/electrospray ionization ion-trap mass spectrometry analysis of chitooligosaccharides by pre-column derivatization with 3-amino-9-ethylcarbazole].
A pre-column derivatization method was established for chitooligosaccharides (COS) analysis, employing 3-amino-9-ethylcarbazole (AEC) as a derivatization reagent. The enamine was generated by the reac...Read More
Synergistic tissue counterstaining and image segmentation techniques for accurate, quantitative immunohistochemistry.
Quantitative analysis of digitized IHC-stained tissue sections is increasingly used in research studies and clinical practice. Accurate quantification of IHC staining, however, is often complicated by...Read More
The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing a single cancer-initiating cel...Read More
The role of hydrogen bond acceptor groups in the interaction of substrates with Pdr5p, a major yeast drug transporter.
The yeast ABC (ATP-binding cassette protein) multidrug transporter Pdr5p transports a broad spectrum of xenobiotic compounds, including antifungal and antitumor agents. Previously, we demonstrated tha...Read More
The study of both the function and development of complex neural systems would be greatly facilitated by a means for systematically blocking intercell communication. One way of preventing cells from s...Read More
Colocalization of NADPH-diaphorase activity and certain neuropeptides in the esophagus of opossum (Didelphis virginiana).
Nitric oxide and various neuropeptides in the myenteric plexus regulate esophageal motility. We sought colocalization of nitric oxide synthase and neuropeptides in frozen sections of mid-portion of sm...Read More
Renal immunomicroscopy using enzyme labeled reagents has been shown to be a reliable method for the identification of immunoglobulins and complement in the routine evaluation of glomerular disease. Ho...Read More
Recommended methods for cytological procedures in haematology. International Committee for Standardization in Haematology (ICSH).
Peroxidase activity in human alveolar macrophages demonstrated by 3-amino-9-ethyl-carbazol as substrate.
Airdried smears of routine sputa, bronchial secretions, and bronchus lavages were incubated to demonstrate peroxidase activity in the alveolar macrophages, with 3-amino-9-ethyl-carbazol as substrate. ...Read More
3-Amino-9-ethylcarbazole (AEC) used as chromogen in immunoperoxidase techniques normally has an intense, red colour. However, as an inconstant phenomenon, a pale yellowish-green reaction product sever...Read More
New hydrosoluble fluorogenic substrates for plasmin gluconoylpeptidyl-3-amido-9-ethylcarbazole were synthesized. The substitution of the N-terminal end of the peptides by a gluconoyl group prevents th...Read More
Multicolour immuno-staining of fibrinogen polypeptide chains for identification of their derivatives in electrophoregrams.
A novel electrophoretic procedure enabling multiple, direct immunoprobing of electrophoregrams without depending on Western blotting is described, and applied to the identification of the derivatives ...Read More
[The sensitivity and specificity of various peroxidase stains--the difference between DAB and 3AC stainings].
FAB Cooperative Group has cited 3 or higher percentage in the peroxidase (PO) activity as one of criteria for the diagnosis of acute myeloid leukemia. We have previously reported in two cases of acute...Read More
Expression of peptidylarginine deiminase-2 and -4, citrullinated proteins and anti-citrullinated protein antibodies in human gingiva.
The presence of citrullinated proteins, and peptidylarginine deiminase types -2 (PAD-2) and -4 (PAD-4) in periodontal tissues, determine the presence of anti-cyclic citrullinated protein antibodies (a...Read More
Chromogen-based immunohistochemical method for elucidation of the coexpression of two antigens using antibodies from the same species.
The authors established a chromogen-based, double immunolabeling method using antibodies from the same species without any unwanted cross-reactivity. In addition, time-consuming staining steps were sh...Read More
The ability to simultaneously visualize expression of multiple antigens in cells and tissues can provide powerful insights into cellular and organismal biology. However, standard methods are limited t...Read More
A comparison of indirect immunofluorescence and the avidin-biotin-peroxidase conjugate technic (ABC) in the performance of the antinuclear antibody test.
Antinuclear antibody tests were performed on 19 patients using both the indirect immunofluorescence procedure (FANA) and the avidin-biotin conjugated immunoperoxidase technic (ABC) on HEp-2 cells. Pea...Read More
A fluorogenic substrate for plasmin, CBZ-Gly-Pro-Arg-AEC, has been synthesized and used to develop a new sensitive photometric and fluorometric assay of plasminogen activator activity. The fluorescenc...Read More
Comparative usefulness of cytology and peroxidase activity in conjunction with carcinoembryonic antigen levels for the diagnosis of mammary cancer.
Diagnosis of cancer via measurement of carcinoembryonic antigen (CEA) levels has been unreliable in early neoplastic stages. In order to improve diagnostic reliability, other cytological parameters we...Read More
Distribution of cholinergic neurons and fibers in the hypothalamus of the rat using choline acetyltransferase as a marker.
The distribution of choline-acetyltransferase-like immunoreactive structures in the rat hypothalamus and preoptic area was examined by using avidin-biotin immunocytochemistry. We found that the hypoth...Read More
Two techniques are described which enable a rapid diagnosis of herpes simplex keratitis to be made. The tests, antibody/antigen reactions, were shown to be accurate and sensitive in the 119 patients e...Read More