|别名||8-苯胺-1-萘磺酸 ;N-苯基-8-萘胺-1-磺酸 N-Phenyl-1-naphthylamine-8-sulfonic Acid Phenyl Acid N-Phenyl peri acid 1,8-ANS ANSA;8-Anilino-1-naphthalenesulfonic acid ;82-76-8; C16H13SO3;苯基周位酸;N-苯基周位酸;N-Phenyl-1-naphthylamine-8-sulfonic Acid Phenyl Acid N-Phenyl peri acid 1,8-ANS ANSA|
|应用||1,8-ANS 是一种荧光染料，对蛋白质的疏水表面具有高亲和力。1,8-ANS经历蓝移和荧光强度显着增加后，结合到低极性区域的蛋白质表面。这些属性使1,8 - ANS 非常适合用于测定亲和力的疏水性配体及其相应的结合蛋白，如具有疏水性的游离脂肪酸的脂肪酸结合蛋白(FABPs)。|
X-ray crystal structure of an FABP inhibitor bound to the active site of aFABP....Read More
We studied the inhibitory effects of isorhamnetin on mushroom tyrosinase by inhibition kinetics and computational simulation. Isorhamnetin reversibly inhibited tyrosinase in a mixed-type manner at Ki=...Read More
The design of therapeutic compounds targeting transthyretin (TTR) is challenging due to the low specificity of interaction in the hormone binding site. Such feature is highlighted by the interactions ...Read More
Detection and analysis of protofibrils and fibrils of hemoglobin: Implications for the pathogenesis and cure of heme loss related maladies.
TFE induces structural alterations of proteins similar to the lipid environment of biological membranes, implicating these studies worthy of analyzing protein conformation in membranes such as red blo...Read More
Trifluoroethanol stabilizes the molten globule state and induces non-amyloidic turbidity in stem bromelain near its isoelectric point.
Stem bromelain (SBM) is a therapeutic protein that has been studied for alkaline denaturation in the intestines, the principal site of its absorption. In this study, we investigated fluorinated alcoho...Read More
Characterization of 4-HNE modified L-FABP reveals alterations in structural and functional dynamics.
4-Hydroxynonenal (4-HNE) is a reactive α,β-unsaturated aldehyde produced during oxidative stress and subsequent lipid peroxidation of polyunsaturated fatty acids. The reactivity of 4-HNE towards DNA...Read More
Crystallographically mapped ligand binding differs in high and low IgE binding isoforms of birch pollen allergen bet v 1.
The ability of pathogenesis-related proteins of family 10 to bind a broad spectrum of ligands is considered to play a key role for their physiological and pathological functions. In particular, Bet v ...Read More
Exploring the unfolding mechanism of γ-glutamyltranspeptidases: The case of the thermophilic enzyme from Geobacillus thermodenitrificans.
γ-glutamyltranspeptidases (γ-GTs) are ubiquitous enzymes that catalyze the hydrolysis of γ-glutamyl bonds in glutathione and glutamine and the transfer of the released γ-glutamyl group to amino ac...Read More
Folding and structural stability are key factors for the proper biological function of proteins. Na(+),K(+)-ATPase is an integral membrane protein involved in the active transport of Na(+) and K(+) ac...Read More
pH-Induced molten globule state of Rhizopus niveus lipase is more resistant against thermal and chemical denaturation than its native state.
Here, we have characterized four pH-dependent states: alkaline state, "B" (pH 9.0), native state, "N" (pH 7.4), acid-induced state, "A" (pH 2.2) and molten globule state,...Read More
Biophysical analysis of apolipoprotein E3 variants linked with development of type III hyperlipoproteinemia.
Apolipoprotein E (apoE) is a major protein of the lipoprotein transport system that plays important roles in lipid homeostasis and protection from atherosclerosis. ApoE is characterized by structural ...Read More
Hydrogen peroxide targets the cysteine at the active site and irreversibly inactivates creatine kinase.
In our study, we showed that at a relatively low concentration, H(2)O(2) can irreversibly inactivate the human brain type of creatine kinase (HBCK) and that HBCK is inactivated in an H(2)O(2) concentr...Read More
pH-dependent conformational transitions in conalbumin (ovotransferrin), a metalloproteinase from hen egg white.
Acid unfolding pathway of conalbumin (CA), a monomeric glycoprotein from hen egg white, has been investigated using far- and near-UV CD spectroscopy, intrinsic fluorescence emission, extrinsic fluores...Read More
Crowding caused by the high concentrations of macromolecules in the living cell changes chemical equilibria, thus promoting aggregation and folding reactions of proteins. The possible magnitude of thi...Read More
The scavenging ability of sufficient divalent metal ions is pivotal for pathogenic bacteria to survive in the host. ATP-binding cassette (ABC)-type metal transporters provide a considerable amount of ...Read More
Application of the Fluorescent Probe 1-Anilinonaphthalene-8-Sulfonate to the Measurement of the Nonspecific Binding of Drugs to Human Liver Microsomes
The fluorescence of 1-anilinonaphthalene-8-sulfonate (ANS) in the presence of human liver microsomes (HLMs) is altered by drugs that bind nonspecifically to the lipid bilayer. The present study charac...Read More
A new class of non-ionic dendronized multiamphiphilic polymers is prepared from a biodegradable (AB)n-type diblock polymer synthesized from 2-azido-1,3-propanediol (azido glycerol) and polyethylene gl...Read More
Fluorescent Methods in the Study of UV-Induced Changes in Structural and Functional State of Human Blood Lymphocytes.
Structural and functional state of human blood lymphocytes after exposure to UV light (240-390 nm) in doses of 151-1359 J/m(2) was studied by methods of laser flow cytofluorometry, indirect immunofluo...Read More
Transcription factors of the peroxisome proliferator-activated receptor (PPAR) family are ligand-activated receptors that play key roles in lipid metabolism and inflammation. The γ isoform (PPARγ) i...Read More
A calcium-dependent interaction between calmodulin and the calponin homology domain of human IQGAP1.
IQGAPs are cytoskeletal scaffolding proteins which collect information from a variety of signalling pathways and pass it on to the microfilaments and microtubules. There is a well-characterised intera...Read More
Principal component analysis of chemical shift perturbation data of a multiple-ligand-binding system for elucidation of respective binding mechanism.
Chemical shift perturbations (CSPs) in NMR spectra provide useful information about the interaction of a protein with its ligands. However, in a multiple-ligand-binding system, determining quantitativ...Read More
Kinetics and thermodynamics of 1-anilino-8-naphthalene sulfonate interactions with Urinary Trypsin Inhibitor.
The interactions between Urinary Trypsin Inhibitor (UTI) and 1-anilino-8-naphthalene sulfonate (ANS) were investigated by fluorescence spectra, isothermal titration calorimetry and molecular modeling....Read More
Reevaluation of ANS binding to human and bovine serum albumins: key role of equilibrium microdialysis in ligand - receptor binding characterization.
In this work we return to the problem of the determination of ligand-receptor binding stoichiometry and binding constants. In many cases the ligand is a fluorescent dye which has low fluorescence quan...Read More
Ligand binding induces a sharp decrease in hydrophobicity of folate binding protein assessed by 1-anilinonaphthalene-8-sulphonate which suppresses self-association of the hydrophobic apo-protein.
High affinity folate binding protein (FBP) regulates as a soluble protein and as a cellular receptor intracellular trafficking of folic acid, a vitamin of great importance to cell growth and division....Read More
Altering APP proteolysis: increasing sAPPalpha production by targeting dimerization of the APP ectodomain.
One of the events associated with Alzheimer's disease is the dysregulation of α- versus β-cleavage of the amyloid precursor protein (APP). The product of α-cleavage (sAPPα) has neuroprotectiv...Read More
[Effect of acetone and the probe 1-anilino-8-naphthalenesulfonate on water as estimated by fluorescence in the UV region].
For investigation of influence of reactive oxygen species on biological systems the peculiarities of light scattering and fluorescence spectra of aqueous solutions in the presence of photoactive addit...Read More
The inability of cells to maintain protein folding homeostasis is implicated in the development of neurodegenerative diseases, malignant transformation, and aging. We find that multiphoton fluorescenc...Read More
The efflux inhibitor phenylalanine-arginine beta-naphthylamide (PAβN) permeabilizes the outer membrane of gram-negative bacteria.
Active efflux of antimicrobial agents is a primary mechanism by which bacterial pathogens can become multidrug resistant. The combined use of efflux pump inhibitors (EPIs) with pump substrates is unde...Read More