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Specific lipids supply critical negative spontaneous curvature--an essential component of native Ca2+-triggered membrane fusion.
The Ca(2+)-triggered merger of two apposed membranes is the defining step of regulated exocytosis. CHOL is required at critical levels in secretory vesicle membranes to enable efficient, native membra...Read More
Monoclonal behavior of molecularly imprinted polymer nanoparticles in capillary electrochromatography.
A new approach based on miniemulsion polymerization is demonstrated for synthesis of molecularly imprinted nanoparticles (MIP-NP; 30-150 nm) with "monoclonal" binding behavior. The performan...Read More
Lipid storage in high-altitude Andean Lakes extremophiles and its mobilization under stress conditions in Rhodococcus sp. A5, a UV-resistant actinobacterium.
The production of triacylglycerols (TAG) or wax esters (WS) seems to be a widespread feature among extremophile bacteria living in high-altitude Andean Lakes (HAAL), Argentina. Twelve out of twenty ba...Read More
Three phenol- and alkanes-degrading bacterial strains were isolated from a freshwater sample. Upon the 16S rRNA gene analysis, phenotype and physiological features, the three strains were designated a...Read More
Conductivity in nonpolar media: experimental and numerical studies on sodium AOT-hexadecane, lecithin-hexadecane and aluminum(III)-3,5-diisopropyl salicylate-hexadecane systems.
The conductivity behavior of doped hydrocarbon systems is studied by applying impedance spectroscopy. In the case of 3,5-diisopropyl salicylato aluminum (III) the charge carriers are formed by dissoci...Read More
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General adhesion behavior of phospholipid vesicles was examined in a wide range of potentials at the mercury electrode by recording time-resolved adhesion signals. It was demonstrated that adhesion-ba...Read More
Liposomes containing a model active component were entrapped within the internal aqueous phase (W(1)) of W(1)/O/W(2) double emulsions, thus providing a double-encapsulation system. Our motivation for ...Read More
We analyzed the production of neutral lipids by the marine hydrocarbonoclastic bacteria Marinobacter sp. strain PAD-2 using hexadecane or succinate as the sole carbon source. Results showed that strai...Read More
Activation thermodynamics of poly(ethylene glycol)-mediated model membrane fusion support mechanistic models of stalk and pore formation.
Membrane fusion, essential to eukaryotic life, is broadly envisioned as a three-step process proceeding from contacting bilayers through two semistable, nonlamellar lipidic intermediate states to a fu...Read More
The partitioning behavior of six petrodiesel/soybean-biodiesel blends (B0, B20, B40, B60, B80, and B100, where B100 is 100% unblended biodiesel) in water was investigated at various oil loads by the 1...Read More
In this work, the first-ever membrane-based single unit operation that enables gravity driven, on-demand separation of various oil-water mixtures is developed. Using this methodology, the on-demand se...Read More
Biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus using oxygen vector and optimum impeller tip speed.
The potential use of n-dodecane and n-hexadecane as oxygen vectors for enhancing hyaluronic acid (HA) biosynthesis by Streptococcus zooepidemicus ATCC 39920 was investigated using a 2-L stirred-tank b...Read More
Simultaneous determination of chloropheniramine maleate and dextromethorphan hydrobromide in plasma sample by hollow fiber liquid phase microextraction and high performance liquid chromatography with the aid of chemometrics.
A simple and high sensitive technique based on three phase hollow fiber liquid phase microextraction (HF-LPME), optimized by using a four-variable experimental design and response surface methodology ...Read More
[Synthesis of surfactants acinetobacter calcoaceticus IMV B-7241 and Rhodococcus erythropolis IMV Ac-5070 in the medium with glycerol].
It was established that glycerol, a byproduct of biodiesel production, may be used as substrate for synthesis of surfactants Rhodococcus erythropolis IMV Ac-5017 and Acinetobacter calcoaceticus IMV B-...Read More
The surface wettabilities of polymer brushes with hydrophobic and hydrophilic functional groups were discussed on the basis of conventional static and dynamic contact angle measurements of water and h...Read More
Hydrocarbon degradation by a new Pseudomonas sp., strain RW-II, with polycationic surfactant to modify the cell hydrophobicity.
Pseudomonas putida RW-II isolated from petroleum refinery wastewater was tested for hydrocarbon degradation potential in the presence of polycationic surfactant, cetyl trimethylammonium bromide (CTAB)...Read More
[Effect of Cu2+ on synthesis of biosurfactants of Acinetobacter calcoaceticus IMV B-7241 and Rhodococcus erythropolis IMV Ac-5017].
Synthesis of biosurfactants (surface-active substances, SAS) was investigated under the conditions of growth of Rhodococcus erythropolis IMV Ac-5017 and Acinetobacter calcoaceticus IMV B-7241 on hydro...Read More
The effect of cultivation conditions (the composition, acidity, and salinity of the cultivation medium; temperature; and the hydrodynamic conditions of cultivation) on the adhesion of actinobacteria o...Read More
Twenty-five strains of filamentous fungi, encompassing 14 different species and belonging mainly to Ascomycetes, were tested for their ability to degrade benzo[a]pyrene (BaP) in mineral liquid medium....Read More
A versatile fluorescence approach to kinetic studies of hydrocarbon autoxidations and their inhibition by radical-trapping antioxidants.
We report a simple coumarin-triarylphosphine conjugate that undergoes fluorescence enhancement upon reaction with hydroperoxides and demonstrate its use to follow autoxidations of 7-dehydrocholesterol...Read More
Hydrocarbon oils such as pristane or hexadecane induce arthritis and lupus in rodents sharing clinical and pathological features with the human diseases rheumatoid arthritis and systemic lupus erythem...Read More
Chain dynamics in a hexadecane melt as seen by neutron scattering and identified by molecular dynamics simulations.
Different local and global chain dynamics in a C(16)H(34) melt could be revealed by resolution resolved time-of-flight quasielastic neutron scattering and complementary molecular dynamics simulations....Read More
Differential proteomic analysis of an engineered Streptomyces coelicolor strain reveals metabolic pathways supporting growth on n-hexadecane.
The alkB gene, encoding an alkane monooxygenase in the actinomycete Gordonia sp. SoCg, was expressed in the non-alkane-degrading actinomycete Streptomyces coelicolor M145. The resulting engineered str...Read More
LadA, a monooxygenase catalyzing the oxidation of n-alkanes to 1-alkanols, is the key enzyme for the degradation of long-chain alkanes (C(15)-C(36)) in Geobacillus thermodenitrificans NG80-2. In this ...Read More