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核糖核酸酶A 来源于牛胰腺(无DNase & Protease)

规格或纯度: ≥2,000 units/mg protein
有货

库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
R128619-1mg
 1mg 现货 Stock Image
R128619-5mg
 5mg 现货 Stock Image
大包装询价 添加到收藏夹 比较  SDS中文版  DATASHEET
main product photo

基本描述

英文别名 9001-99-4|Nuclease, ribo-|L-HISTIDINE, N-beta-ALANYL-|2-(3-aminopropanoylamino)-3-(1H-imidazol-5-yl)propanoic acid|9001-73-4|DL-(3-aminopropanoyl)histidine|108333-82-0|2-(3-aminopropanamido)-3-(1H-imidazol-5-yl)propanoic acid|beta-Alanylhistidine|L-Histid
规格或纯度 ≥2,000 units/mg protein
英文名称 Ribonuclease A from bovine pancreas(DNase & Protease Free)
单位定义 One Kalnitsky Unit causes an increase in absorbance of 1.0 at 260nm at 37°C and pH 5.0 when yeast ribosomal RNA is hydrolyzed to acid soluble oliognucleotides.
储存温度 -20°C储存
运输条件 超低温冰袋运输
产品介绍

Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A. Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A. Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

名称和标识符

酶学委员会编号 3.1.27.5
Isomeric SMILES C1=C(NC=N1)CC(C(=O)O)NC(=O)CCN
PubChem CID 9369

化学和物理性质

敏感性 对湿度敏感

安全和危险性（GHS）

象形图
ghs08

Health Hazard
信号词 Danger
危险声明 H334: May cause allergy or asthma symptoms or breathing difficulties if inhaled
预防措施声明 P261,P501,P284,P304+P340,P342+P316

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