Mouse monoclonal (C209) to ATP1A1 could be uesd for WB, IHC and so on. Application: Western blotting: 0.5-2µg/mL Immunohistochemistry: 5-20µg/mL Protein Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Post-translational Phosphorylation on Tyr-10 modulates pumping activity.
Recombinant ATP1a1 (His493~Ala660) expressed in E.coli
阳性对照
WB: HEK293, Hep G2 and C6 cell lysates. IHC: Human Placenta Tissue, Human Liver cancer Tissue, Human Kidney Tissue, Porcine Kidney Tissue, Rat Uterus Tissue, Rat Small intestine Tissue.
ATP1A1 Mouse mAb (Ab088306) - Western Blot All lanes: ATP1A1 Mouse mAb (Ab088306) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 100 kDa Observed band size: 95 kDa Exposure time: 117.2 s
ATP1A1 Mouse mAb (Ab088306) - Western Blot All lanes: ATP1A1 Mouse mAb (Ab088306) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 100 kDa Observed band size: 95 kDa Exposure time: 30.0 s
ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Human Placenta Tissue Primary Ab: 10 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Human Liver cancer Tissue Primary Ab: 10 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 10 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Porcine Kidney Tissue Primary Ab: 20 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Rat Uterus Tissue Primary Ab: 3 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Rat Small intestine Tissue Primary Ab: 3 ug/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.