Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Post-translational: Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
宿主种属
兔(Rabbit)
特异性
beta III Tubulin
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human beta III Tubulin (AA 400-450).
阳性对照
WB: HeLa, Hep G2,and A375 cell lysates. IHC: Human thyroid cancer tissue. IF/ICC: HeLa, A431 cells.
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant beta III Tubulin Antibody (Ab091169) - Western Blot All lanes: Recombinant beta III Tubulin Antibody (Ab091169) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa Observed band size: 51 kDa Exposure time: 15.6 s
Recombinant beta III Tubulin Antibody (Ab091169) - Western Blot All lanes: Recombinant beta III Tubulin Antibody (Ab091169) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa Observed band size: 53 kDa Exposure time: 100.0 s
Recombinant beta III Tubulin Antibody (Ab091169) - Western Blot All lanes use Recombinant beta III Tubulin Antibody (Ab091169) at 1/10000 dilution for 1 hour at room temperature.
Recombinant beta III Tubulin Antibody (Ab091169) - IHC Immunohistochemical analysis of paraffin-embedded human thyroid cancer, using Recombinant beta III Tubulin Antibody (Ab091169).
Recombinant beta III Tubulin Antibody (Ab091169) - IF/ICC Immunofluorescent analysis of HeLa cells, using the Recombinant beta III Tubulin Antibody (Ab091169) at 1/50 dilution.
Recombinant beta III Tubulin Antibody (Ab091169) - IF/ICC Immunofluorescent analysis of HeLa cells, using the Recombinant beta III Tubulin Antibody (Ab091169) at 1/50 dilution.
Recombinant beta III Tubulin Antibody (Ab091169) - IF/ICC Immunofluorescent analysis of A431 cells, using Recombinant beta III Tubulin Antibody (Ab091169) .
ZJ25F1129809
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