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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
CXC 趋化因子配体 12(CXCL12)又称基质细胞衍生因子 1(SDF1),是一种 趋化因子蛋白,由 CXCL12 基因编码。它在许多组织和细胞类型中普遍表达。 基质细胞源性因子 1-α和 1-β是属于趋化因子家族的小细胞因子,其成员激活白细胞,通常由促炎症刺激物如脂多糖、TNF 或 IL1 诱发。趋化因子的特点是存在 4 个保守的半胱氨酸,形成 2 个二硫键。它们可以被分为 2 个亚家 族。在 CC 亚家族中,半胱氨酸残基是相互毗邻的。在 CXC 亚家族中,它们被一个中间的氨基酸分开。SDF1 蛋白属于后一组。它的信号传导已在几种癌症中被观察到。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人基质细胞衍生因子1(SDF-1a;CXCL12)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人基质细胞衍生因子1(SDF-1a;CXCL12)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human SDF-1a;CXCL12. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human SDF-1a;CXCL12 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.