计算溶液所需的质量、体积或浓度。
ActiBioPure™,EnzymoPure™,生物活性,重组,高性能 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Acyl-CoA oxidase is an FAD-dependent enzyme that catalyzes the oxidation of acyl-CoA to produce α,β-enoyl-CoA and H₂O₂.
Application: For in vitro research.
Assay Procedure
Principle

Unit Definition
Reagents
Dilution solution: Add 2.3 g potassium dihydrogen phosphate and 22.8 g potassium hydrogen phosphate to 800 mL deionized water, stir to dissolve, adjust pH to 7.60 with potassium hydroxide, and make up to 1 L.
Reagent A: Dissolve 24.23 grams of Tris in 800 mL of water, stir until fully dissolved, then adjust the pH to 8.00 with hydrochloric acid at 25°C.
Reagent B: 15 mM 4-AA solution (0.305 g of 4-AA dissolved in 100 mL water and thoroughly mixed).
Reagent C: 0.2% (W/V) phenol solution (2 grams of phenol dissolved in 1 liter of water);
Reagent D: A 50 U/ml POD solution (diluted by 100 mL of Reagent A for 5kU enzyme);
Reagent E: 1% Triton X-100 solution (dissolve 10 g Triton X-100 in 1 L water);
Reagent F: Dissolve 10 mg of palmitoyl-CoA in 2 mL of diluent;
Reagent G (Acetyl-CoA Oxidase Dilution): Before use, prepare the dilution solution from 3.1 by adding FAD monosodium salt to achieve 8 mg/L and ATP to 1.52 g/L. This solution is the Acetyl-CoA Oxidase Dilution.
Test sample: Weigh the required enzyme with a Weighing tube before testing. When weighing the enzyme, at least 10mg of enzyme should be weighed to ensure the accuracy of weighing. Dissolve the enzyme with enzyme dilution solution and adjust the volume to the preparation volume.
Procedure
Calculation

Vt: Total volume of the reaction mixture (ml)
ε: Molar extinction coefficient, with a value of 12 (cm²/μmol)
Vs: Volume of the enzyme solution to be tested (ml)
d: Light path length of the cuvette (cm)
D: Dilution factor of the enzyme solution
△A/min: △A/min(test sample)-△A/min(blank) (ABS/min)
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | R1506899 | |
| 分析证书 | R1506899 | |
| 分析证书 | R1506899 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Bioactive 级别指南 → 查看 Recombinant 级别指南 → 查看 ActiBioPure™ 级别指南 → 查看 High Performance 级别指南 → 查看 EnzymoPure™ 级别指南 →