酰基辅酶A氧化酶 (ACO)

CAS: 61116-22-1 货号: R1506899 EC号: 838-598-8
有货
级别和纯度: 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 重组 ? 重组(Recombinant)—— 通过重组表达生产,序列确定、批次稳定。适用于来源明确、无动物源的可重现蛋白。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 高性能 ? 高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。 EnzymoPure™ ? EnzymoPure™ —— 阿拉丁的高质量酶解决方案系列。当酶纯度和明确活性决定检测或工艺性能时使用。 8-12 U/mg enzyme powder
Accession #
Q9R0H0,Q9EPL9
表达系统
E. coli
生物活性
8-12 U/mg enzyme powder
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规格
库存
价格
数量
100U
R1506899-100U
现货 Stock Image
¥169.90
500U
R1506899-500U
现货 Stock Image
¥479.90
1KU
R1506899-1KU
现货 Stock Image
¥799.90
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为什么选择此级别

ActiBioPure™,EnzymoPure™,生物活性,重组,高性能 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


Acyl-CoA oxidase is an FAD-dependent enzyme that catalyzes the oxidation of acyl-CoA to produce α,β-enoyl-CoA and H₂O₂. 

Application: For in vitro research.

Assay Procedure

Principle


Unit Definition

One unit of enzyme activity equals the amount of enzyme required to produce 1 micromole of H₂O₂ (equivalent to 1/2 micromole of quinone dye) within 1 minute under specified reaction conditions.

Reagents

Dilution solution: Add 2.3 g potassium dihydrogen phosphate and 22.8 g potassium hydrogen phosphate to 800 mL deionized water, stir to dissolve, adjust pH to 7.60 with potassium hydroxide, and make up to 1 L.

Reagent A: Dissolve 24.23 grams of Tris in 800 mL of water, stir until fully dissolved, then adjust the pH to 8.00 with hydrochloric acid at 25°C.

Reagent B: 15 mM 4-AA solution (0.305 g of 4-AA dissolved in 100 mL water and thoroughly mixed).

Reagent C: 0.2% (W/V) phenol solution (2 grams of phenol dissolved in 1 liter of water);

Reagent D: A 50 U/ml POD solution (diluted by 100 mL of Reagent A for 5kU enzyme);

Reagent E: 1% Triton X-100 solution (dissolve 10 g Triton X-100 in 1 L water);

Reagent F: Dissolve 10 mg of palmitoyl-CoA in 2 mL of diluent;

Reagent G (Acetyl-CoA Oxidase Dilution): Before use, prepare the dilution solution from 3.1 by adding FAD monosodium salt to achieve 8 mg/L and ATP to 1.52 g/L. This solution is the Acetyl-CoA Oxidase Dilution.

Test sample: Weigh the required enzyme with a Weighing tube before testing. When weighing the enzyme, at least 10mg of enzyme should be weighed to ensure the accuracy of weighing. Dissolve the enzyme with enzyme dilution solution and adjust the volume to the preparation volume.

Procedure

Add the following reagents to the cuvette in the specified proportions: 0.2 mL of Reagent A, 0.1 mL of Reagent B, 0.1 mL of Reagent C, 0.1 mL of Reagent D, 0.1 mL of Reagent E, 0.1 mL of Reagent F, and 0.3 mL of deionized water. After preheating at 37°C for 300 seconds, add 0.02 mL of the enzyme solution to be tested. Measure the absorbance change rate within 1 minute at a wavelength of 500 nm. The linear range of this method is 0.2-0.5ku/L.

Calculation

The enzyme activity is calculated using the following formula:

Vt: Total volume of the reaction mixture (ml)

ε: Molar extinction coefficient, with a value of 12 (cm²/μmol)

Vs: Volume of the enzyme solution to be tested (ml)

d: Light path length of the cuvette (cm)

D: Dilution factor of the enzyme solution

△A/min: △A/min(test sample)-△A/min(blank) (ABS/min)

规格

产品名称
酰基辅酶A氧化酶 (ACO)
英文别名
acyl-CoA oxidase | Acox1 | Acox3 | AOX | Acox | Paox | D130055E20Rik | PCOX | EST-s59
规格或纯度
生物活性,重组,ActiBioPure™,高性能,EnzymoPure™,8-12 U/mg enzyme powder
生物活性
8-12 U/mg enzyme powder
表达系统
E. coli
来源
重组表达
CAS编号和信息
61116-22-1
酶学委员会编号
EC 1.3.3.6
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
浓度
8-12 U/mg enzyme powder
复溶
Reconstitute in 8 mg/L FAD monosodium salt and 1.52 g/L ATP.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃(12个月);复溶后建议分装,避免反复冻融。
单位定义
One unit of enzyme activity equals the amount of enzyme required to produce 1 micromole of H₂O₂ (equivalent to 1/2 micromole of quinone dye) within 1 minute under specified reaction conditions.

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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✅ 分析证书 (COA)

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📊 产品数据表

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ26F0434110 分析证书 R1506899
ZJ26F0434109 分析证书 R1506899
ZJ26F0434108 分析证书 R1506899
技术文档和文章
溶液计算器

常见问题

本产品的 CAS 号、分子式和分子量是多少?
本产品的 CAS 号为 61116-22-1。