| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| rp142573-10μg |
10μg |
现货  |
| |
| rp142573-50μg |
50μg |
现货 |
| |
| rp142573-100μg |
100μg |
现货 |
| |
| rp142573-1mg |
1mg |
期货 |
|
| 产品名称 | Recombinant Human Adiponectin/Acrp30 Protein |
|---|---|
| 别名 | 重组人脂联素蛋白 | 重组人脂联素/Acrp30 蛋白 |
| 英文别名 | ACDC | Acrp30 | ACRP30ADPN | adipocyte, C1Q and collagen domain containing | Adiponectin | adiponectin, C1Q and collagen domain containing | AdipoQ | ADIPQTL1 | ApM1 | apM-1 | C1q and collagen domain-containing protein | GBP28 | GBP28apM1 | Gelatin-bindin |
| 规格或纯度 | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE) |
| 产品介绍 |
Purity ＞95% SDS-PAGE. Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. Post-translational Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes. O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. |
| 生物活性 | Measured by its ability to induce TIMP-1 secretion by mouse macrophages. The ED₅₀for this effect is 1-5 µg/mL. |
| 内毒素水平 | <0.1 EU/μg |
| 表达系统 | HEK293 |
| 种属 | Human |
| 氨基酸 | 19-244 aa |
| 序列 | ETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTNHHHHHH |
| 纯度 | ＞95%(SDS-PAGE) |
| 蛋白标签 | C-His |
| N-末端序列 | Glu |
| 无载体 | Yes |
| 无动物源 | Yes |
| Accession # | Q15848 |
| 来源 | 重组表达 |
| 预测分子量 | 25 kDa |
Recombinant Human Adiponectin/Acrp30 Protein (rp142573) - Protein Bioactivity
Fully biologically active determined by the dose dependent increase in MCF7 cell proliferation. The ED₅₀ for this effect is 0.18 μg/mL. Adiponectin induces a dichotomic effect on breast cancer growth. It stimulates growth in ERα+ MCF-7 cells while inhibiting proliferation of ERα- MDA-MB-231 cells.
Recombinant Human Adiponectin/Acrp30 Protein (rp142573) - Protein Bioactivity
Measured by its ability to induce TIMP-1 secretion by mouse macrophages. The ED₅₀ for this effect is 2.14 µg/mL.
Recombinant Human Adiponectin/Acrp30 Protein (rp142573) - SDS-PAGE
3 μg/lane of Recombinant Human Adiponectin/Acrp30 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 31.2 kDa under reducing conditions and 30.2 & 55.0 kDa under non-reducing conditions.
| 物理形态 | 冻干(Lyophilized) |
|---|---|
| 储存缓冲液 | 10mM PBS (PH7.4), containing 5% trehalose, 5% mannitol |
| 复溶 | Reconstitute in water to a concentration of 0.1-1.0 mg/ml. |
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 在 -20~-80℃ 下可存放 1 年以上 |
| 分子类型 | 蛋白质 |
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ25F0116518 |
分析证书 | 25-01-08 | rp142573 |
| ZJ23F1101660 |
分析证书 | 23-11-20 | rp142573 |
| ZJ23F1101658 |
分析证书 | 23-11-20 | rp142573 |
| ZJ23F1101657 |
分析证书 | 23-11-20 | rp142573 |
| 1. Maeda K, Okubo K, Shimomura I, Funahashi T, Matsuzawa Y, Matsubara K. (1996) cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1).. Biochem Biophys Res Commun, 221 (2): (286-9). [PMID:8619847] [10.1021/op500134e] |
| 2. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334] [10.1021/op500134e] |
| 3. Nakano, Y Y, Tobe, T T, Choi-Miura, N H NH, Mazda, T T and Tomita, M M.. (1996) Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma.. Journal of biochemistry, [PMID:8947845] |
| 4. Saito, K K and 8 more authors.. (1999) Organization of the gene for gelatin-binding protein (GBP28).. Gene, (18): [PMID:10095105] |
| 5. Schäffler, A A and 7 more authors.. (1999) The human apM-1, an adipocyte-specific gene linked to the family of TNF's and to genes expressed in activated T cells, is mapped to chromosome 1q21.3-q23, a susceptibility locus identified for familial combined hyperlipidaemia (FCH).. Biochemical and biophysical research communications, (5): [PMID:10403784] |
| 6. Takahashi, M M and 13 more authors.. (2000) Genomic structure and mutations in adipose-specific gene, adiponectin.. International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity, [PMID:10918532] |
| 7. Yokota, T T and 10 more authors.. (2000) Adiponectin, a new member of the family of soluble defense collagens, negatively regulates the growth of myelomonocytic progenitors and the functions of macrophages.. Blood, (1): [PMID:10961870] |
| 8. Ouchi, N N and 14 more authors.. (2000) Adiponectin, an adipocyte-derived plasma protein, inhibits endothelial NF-kappaB signaling through a cAMP-dependent pathway.. Circulation, (12): [PMID:10982546] |
| 9. Yamauchi, T T and 24 more authors.. (2001) The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity.. Nature medicine, [PMID:11479627] |
| 10. Hara, Kazuo K and 23 more authors.. (2002) Genetic variation in the gene encoding adiponectin is associated with an increased risk of type 2 diabetes in the Japanese population.. Diabetes, [PMID:11812766] |
| 11. Kondo, Hidehiko H and 11 more authors.. (2002) Association of adiponectin mutation with type 2 diabetes: a candidate gene for the insulin resistance syndrome.. Diabetes, [PMID:12086969] |
| 12. Vasseur, Francis F and 14 more authors.. (2002) Single-nucleotide polymorphism haplotypes in the both proximal promoter and exon 3 of the APM1 gene modulate adipocyte-secreted adiponectin hormone levels and contribute to the genetic risk for type 2 diabetes in French Caucasians.. Human molecular genetics, (1): [PMID:12354786] |
| 13. Waki, Hironori H and 12 more authors.. (2003) Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin.. The Journal of biological chemistry, (10): [PMID:12878598] |
| 14. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161] |
| 15. Richards, Ayanthi A AA and 6 more authors.. (2006) Adiponectin multimerization is dependent on conserved lysines in the collagenous domain: evidence for regulation of multimerization by alterations in posttranslational modifications.. Molecular endocrinology (Baltimore, Md.), [PMID:16497731] |
| 16. Richards, Ayanthi A AA and 13 more authors.. (2010) Sialic acid modification of adiponectin is not required for multimerization or secretion but determines half-life in circulation.. Molecular endocrinology (Baltimore, Md.), [PMID:19855092] |
| 17. Min, Xiaoshan X and 7 more authors.. (2012) Crystal structure of a single-chain trimer of human adiponectin globular domain.. FEBS letters, (23): [PMID:22449980] |
| 18. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912] |
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