Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED50 for this effect is 0.1‑0.4 µg/mL in the presence of Recombinant Mouse Klotho and heparin.
Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Up-regulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.
Post-translational: Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases. O-glycosylated at Thr-171 and Thr-178 by GALNT3 and glycosylation of Thr-178 requires previous glycosylation at Thr171. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23. Phosphorylation at Ser-180 mediated by FAM20C slows down glycosylation at Thr-178 notably.
生物活性
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED50 for this effect is 0.1‑0.4 µg/mL in the presence of Recombinant Mouse Klotho and heparin.
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
浓度
见COA
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20~-80℃（1年）；复溶后建议分装，避免反复冻融。
图片
Recombinant Human FGF-23 Protein (rp145903) - Protein Bioactivity
Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.33 μg/mL in the presence of 1.0 μg/mL Recombinant Mouse Klotho beta Protein (rp184968).
Recombinant Human FGF-23 Protein (rp145903) - SDS-PAGE
2 μg/lane of Recombinant Human FGF-23 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 30 kDa.
ZJ25F1230551
危险品化学品经营许可证（带存储）