Recombinant Human IL-2 Protein

货号 (SKU)	包装规格	是否现货
rp147476-10μg (试用装) 申请此免费试用装（?）作为尊贵的客户，您每年可免费申请一次试用装产品，尽享探索与享受！	10μg	现货
rp147476-50μg	50μg	现货
rp147476-100μg	100μg	现货
rp147476-1mg	1mg	期货

基本描述

产品名称	Recombinant Human IL-2 Protein
别名	重组人白细胞介素-2蛋白
英文别名	IL-2 \| T-cell growth factor \| TCGF
规格或纯度	GMP, ≥95% (SDS-PAGE, SEC-HPLC), Active, CHO-K1, His, 21-153 aa
产品介绍	白介素2即白细胞介素-2（interleukin-2，IL-2），又名T细胞生长因子(T cell growth factor，TCRF)。主要由活化的CD4+T细胞和CD8+T细胞产生的具有广泛生物活性的细胞因子。是所有T细胞亚群的生长因子，能使T细胞在试管内长期存活，并可促进活化B细胞增殖，故为调控免疫应答的重要因子，也参与抗体反应、造血和肿瘤监视。本产品由真核细胞表达的重组蛋白，是将蛋白基因接入稳定表达载体，转化CHO-K1细胞，经克隆筛选，质量验证，挑选的稳定表达株，由化学成分确定的培养基（不含有任何动物源成分）发酵制备，工艺稳定，保证了产品质量及批次间的一致性。本产品为无菌冻干粉剂，由含有 10mM 磷酸盐pH为 7.2的蛋白溶液经0.2μm过滤后分装冻干。 Purity ≥95% (SDS-PAGE, SEC-HPLC) Additional sequence information 133 amino acids Function Interleukin-2, or interleukin-2, is also known as T cell growth factor (TCRF). A wide range of bioactive cytokines produced primarily by activated CD4+T cells and CD8+T cells. It is a growth factor of all T cell subsets, which can make T cells survive in vitro for a long time and promote the proliferation of activated B cells. Therefore, it is an important factor in the regulation of immune response, and also involved in antibody response, hematopoietic and tumor surveillance. Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. This product is a recombinant protein expressed by eukaryotic cells. The protein gene was inserted into stable expression vector and transformed into CHO-K1 cells. After cloning, screening and quality verification, the selected stable expression strain was prepared by fermentation of the medium determined by chemical composition (without any animal source components). This product is a sterile lyophilized powder, made from a protein solution containing 10mM phosphate with a pH of 7.2 filtered by 0.2μm.
生物活性	Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is typically <2.5ng/mL.
表达系统	CHO-K1
种属	Human
氨基酸	Amino acids
序列	APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT
纯度	≥95% (SDS-PAGE, SEC-HPLC)
蛋白标签	His
蛋白长度	Full length protein
无载体
Accession #	P60568
来源	Recombinant
预测分子量	15.4 kDa
SDS-PAGE	13.0 kDa, under reducing conditions; 13.0 kDa, under non-reducing conditions.

产品规格参数

物理外观	Lyophilized
储存缓冲液	含有 10mM 磷酸盐pH为 7.2的蛋白溶液经0.2μm过滤后分装冻干
复溶	建议将冻干 rhIL-2溶解在注射用水或灭菌的超纯水中，浓度不低于100μg/ml，以待进一步稀释至工作浓度。尽可能避免反复冻融。复溶后的细胞因子：4℃可稳定储存7-10天；短期保存请分装后存放于-20℃。
储存温度	-20°C储存
运输条件	超低温冰袋运输
稳定性与储存	Shipped at 4°C. Store at -20°C. Lyophilized with no additives. This product is an active protein and may elicit a biological response in vivo, handle with caution.

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Protein Bioactivity: Recombinant Human IL-2 Protein (rp147476)

Recombinant Human IL-2 Protein (rp147476)-Protein Bioactivity
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is typically <2.5ng/mL.

SDS-PAGE: Recombinant Human IL-2 Protein (rp147476)

Recombinant Human IL-2 Protein (rp147476) -SDS-PAGE
3μg/lane of Recombinant Human IL-2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 13.0 kDa.

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参考文献

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Recombinant Human IL-2 Protein

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基本描述

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