Recombinant Human IL-2 Protein(Active)

功能和特点
  • 规格或纯度: GMP, ≥95% (SDS-PAGE, SEC-HPLC), Active, CHO-K1, His, 21-153 aa
  • 生物活性: Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is typically <2.5ng/mL.
  • 蛋白标签: His
有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
rp147476-10μg (试用装)
申请此免费试用装(?)
作为尊贵的客户,您每年可免费申请三次您选择的不同货号试用装产品,尽享探索与享受!
10μg 现货 Stock Image
rp147476-50μg
50μg 现货 Stock Image
rp147476-100μg
100μg 现货 Stock Image
rp147476-1mg
1mg 期货 Stock Image
查看相关系列
Accession#:P60568 Gene ID:3558 IL2

基本描述

产品名称 Recombinant Human IL-2 Protein
别名 重组人白细胞介素-2蛋白
规格或纯度 GMP, ≥95% (SDS-PAGE, SEC-HPLC), Active, CHO-K1, His, 21-153 aa
产品介绍


白介素2即白细胞介素-2(interleukin-2,IL-2),又名T细胞生长因子(T cell growth factor,TCRF)。主要由活化的CD4+T细胞和CD8+T细胞产生的具有广泛生物活性的细胞因子。是所有T细胞亚群的生长因子,能使T细胞在试管内长期存活,并可促进活化B细胞增殖,故为调控免疫应答的重要因子,也参与抗体反应、造血和肿瘤监视。 

本产品由真核细胞表达的重组蛋白,是将蛋白基因接入稳定表达载体,转化CHO-K1细胞,经克隆筛选,质量验证,挑选的稳定表达株,由化学成分确定的培养基(不含有任何动物源成分)发酵制备,工艺稳定,保证了产品质量及批次间的一致性。

本产品为无菌冻干粉剂,由含有 10mM 磷酸盐pH为 7.2的蛋白溶液经0.2μm过滤后分装冻干。


Purity

≥95% (SDS-PAGE, SEC-HPLC) 


Additional sequence information

133 amino acids


Function

Interleukin-2, or interleukin-2, is also known as T cell growth factor (TCRF). A wide range of bioactive cytokines produced primarily by activated CD4+T cells and CD8+T cells. It is a growth factor of all T cell subsets, which can make T cells survive in vitro for a long time and promote the proliferation of activated B cells. Therefore, it is an important factor in the regulation of immune response, and also involved in antibody response, hematopoietic and tumor surveillance.

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.


This product is a recombinant protein expressed by eukaryotic cells. The protein gene was inserted into stable expression vector and transformed into CHO-K1 cells. After cloning, screening and quality verification, the selected stable expression strain was prepared by fermentation of the medium determined by chemical composition (without any animal source components).

This product is a sterile lyophilized powder, made from a protein solution containing 10mM phosphate with a pH of 7.2 filtered by 0.2μm.


生物活性 Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is typically <2.5ng/mL.
种属 Human
氨基酸 Amino acids
序列 APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT
纯度 ≥95% (SDS-PAGE, SEC-HPLC)
蛋白标签 His
蛋白长度 Full length protein
无载体 Yes
Accession # P60568
来源 Recombinant
预测分子量 15.4 kDa
SDS-PAGE 13.0 kDa, under reducing conditions; 13.0 kDa, under non-reducing conditions.

产品规格参数

物理外观 Lyophilized
储存缓冲液 含有 10mM 磷酸盐pH为 7.2的蛋白溶液经0.2μm过滤后分装冻干
复溶 建议将冻干 rhIL-2溶解在注射用水或灭菌的超纯水中,浓度不低于100μg/ml,以待进一步稀释至工作浓度。尽可能避免反复冻融。 复溶后的细胞因子:4℃可稳定储存7-10天;短期保存请分装后存放于-20℃。
储存温度 -20°C储存
运输条件 超低温冰袋运输
稳定性与储存 Shipped at 4°C. Store at -20°C. Lyophilized with no additives. This product is an active protein and may elicit a biological response in vivo, handle with caution.

图片

Recombinant Human IL-2 Protein (rp147476)-Protein Bioactivity
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is typically <2.5ng/mL.

Recombinant Human IL-2 Protein (rp147476) -SDS-PAGE
3μg/lane of Recombinant Human IL-2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 13.0 kDa.

相关文档

质检报告COA

请输入批号:


产品问答

产品问答

登录提交问题 Hover me 请先登录再提交问题
您提交该产品问题后,我们会在1-2个工作日内给您答复,您可以登录"我的账号",然后点击"我的产品问答"查看答案