Recombinant Human IFN-alpha-2B Protein

货号 (SKU)	包装规格	是否现货
rp156087-10μg	10μg	期货
rp156087-100μg	100μg	现货
rp156087-1mg	1mg	期货

基本描述

产品名称	Recombinant Human IFN-alpha-2B Protein
英文别名	IFN-alpha-2 \| Interferon alpha-A \| LeIF A
规格或纯度	Animal Free, >98% ( SDS-PAGE and HPLC), Active, Yeast, No tag, 24-188 aa
产品介绍	干扰素（IFN）以其在宿主防御机制中的关键作用而闻名。Ⅰ型干扰素包括IFN-alpha 家族，IFN-beta， -omega，-kappa 和 Limitin/IFN-zeta，对调节病毒感染的免疫应答具有重要意义。Ⅱ型干扰素组仅包括干扰素γ。IFN-γ是一种多功能细胞因子，具有抗增殖、免疫调节和促炎症作用，对免疫应答的许多方面都很重要。Ⅲ型干扰素家族包括IL-29/IFN-lambda 1， IL-28A/IFN-lambda 2， IL-28B/IFN-lambda 3， and IFN-lambda 4。与Ⅰ型干扰素类似，Ⅲ型干扰素具有抗病毒、抗增殖和免疫调节作用。 IFN-αs are proteins secreted by leukocyte. They are mainly involved in innate immune response against viral infection. The IFN-α family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.
生物活性	Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.6 × 10^8 IU/mg.
表达系统	Yeast
种属	Human
氨基酸	24-188 aa
序列	CDLPQTHSLG SRRTLMLLAQ MRRISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE
纯度	＞98% ( SDS-PAGE and HPLC)
蛋白标签	No tag
蛋白长度	Full length protein
无载体
无动物源
Accession #	P01563
来源	Recombinant
预测分子量	19.3 kDa

产品规格参数

物理外观	Lyophilized
储存缓冲液	Lyophilized from a 0.2 µm filtered solution in 1×PBS, pH 7.4, with 0.02 % Tween-20.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Upon delivery aliquot, Store at -20°C, Avoid freeze / thaw cycles. Lyophilized with no additives. This product is an active protein and may elicit a biological response in vivo, handle with caution.

质检证书(COA)

质检报告(COA)

输入批号以搜索COA:

图片

Protein Bioactivity: Recombinant Human IFN-alpha-2B Protein (rp156087)

Recombinant Human IFN-alpha-2B Protein (rp156087)-Protein Bioactivity
Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.6×10⁸ IU/mg.

SDS-PAGE: Recombinant Human IFN-alpha-2B Protein (rp156087)

Recombinant Human IFN-alpha-2B Protein (rp156087)-SDS-PAGE
Recombinant Human IFN-alpha-2B Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. Showing a single band at 19.3 kDa under reducing conditions and 16.2 kDa under non-reducing conditions.

产品问答

登录提交问题

查看全部

您提交该产品问题后,我们会在1-2个工作日内给您答复,您可以登录"我的账号",然后点击"我的产品问答"查看答案

参考文献

1. Adolf, G R GR, Kalsner, I I, Ahorn, H H, Maurer-Fogy, I I and Cantell, K K.. (1991) Natural human interferon-alpha 2 is O-glycosylated.. The Biochemical journal, (1): (). [PMID:2049076]
2. Oliver, G G, Balbás, P P, Valle, F F, Soberón, X X and Bolívar, F F.. () [Cloning of human leukocyte interferon cDNA and a strategy for its production in E. coli].. Revista latinoamericana de microbiologia, (): (). [PMID:3906813]
3. Streuli, M M, Nagata, S S and Weissmann, C C.. (1980) At least three human type alpha interferons: structure of alpha 2.. Science (New York, N.Y.), (19): (). [PMID:6158094]
4. Allen, G G and Fantes, K H KH.. (1980) A family of structural genes for human lymphoblastoid (leukocyte-type) interferon.. Nature, (2): (). [PMID:6159537]
5. Goeddel, D V DV and 17 more authors.. (1980) Human leukocyte interferon produced by E. coli is biologically active.. Nature, (2): (). [PMID:6159538]
6. and Wetzel, R R.. (1981) Assignment of the disulphide bonds of leukocyte interferon.. Nature, (12): (). [PMID:6162107]
7. Goeddel, D V DV and 9 more authors.. (1981) The structure of eight distinct cloned human leukocyte interferon cDNAs.. Nature, (5): (). [PMID:6163083]
8. Lawn, R M RM and 5 more authors.. (1981) DNA sequence of a major human leukocyte interferon gene.. Proceedings of the National Academy of Sciences of the United States of America, (): (). [PMID:6170983]
9. Weber, H H and Weissmann, C C.. (1983) Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli.. Nucleic acids research, (25): (). [PMID:6310510]
10. Lee, N N and 7 more authors.. (1995) Interferon-alpha 2 variants in the human genome.. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, (): (). [PMID:7627809]
11. Murgolo, N J NJ and 9 more authors.. (1993) A homology model of human interferon alpha-2.. Proteins, (): (). [PMID:8234245]
12. Radhakrishnan, R R and 6 more authors.. (1996) Zinc mediated dimer of human interferon-alpha 2b revealed by X-ray crystallography.. Structure (London, England : 1993), (15): (). [PMID:8994971]
13. Klaus, W W, Gsell, B B, Labhardt, A M AM, Wipf, B B and Senn, H H.. (1997) The three-dimensional high resolution structure of human interferon alpha-2a determined by heteronuclear NMR spectroscopy in solution.. Journal of molecular biology, (12): (). [PMID:9417943]
14. Nyman, T A TA, Tölö, H H, Parkkinen, J J and Kalkkinen, N N.. (1998) Identification of nine interferon-alpha subtypes produced by Sendai virus-induced human peripheral blood leucocytes.. The Biochemical journal, (15): (). [PMID:9425112]
15. Austruy, E E and 7 more authors.. () A defective retroviral vector encoding human interferon-alpha2 can transduce human leukemic cell lines.. Cancer gene therapy, (): (). [PMID:9694076]
16. Humphray, S J SJ and 147 more authors.. (2004) DNA sequence and analysis of human chromosome 9.. Nature, (27): (). [PMID:15164053]
17. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): (). [PMID:16959974]
18. Quadt-Akabayov, Sabine R SR, Chill, Jordan H JH, Levy, Rina R, Kessler, Naama N and Anglister, Jacob J.. (2006) Determination of the human type I interferon receptor binding site on human interferon-alpha2 by cross saturation and an NMR-based model of the complex.. Protein science : a publication of the Protein Society, (): (). [PMID:17001036]
19. Gull, Iram I, Samra, Zahoor Qadir ZQ, Aslam, Muhammad Shahbaz MS and Athar, Muhammad Amin MA.. (2013) Heterologous expression, immunochemical and computational analysis of recombinant human interferon alpha 2b.. SpringerPlus, (): (). [PMID:23875128]

Recombinant Human IFN-alpha-2B Protein

库存信息

库存信息

库存信息

基本描述

产品规格参数

质检证书(COA)

质检报告(COA)

图片

相关文档

质检报告COA

相关技术文章

产品问答

产品问答

参考文献