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基本描述
| 别名 | 小RNA梯状图 |
|---|---|
| 规格或纯度 | 10-50nt, 9 bands |
| 稳定性与储存 | -80℃保存，至少一年有效。 |
| 英文名称 | Small RNA Ladder |
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 产品介绍 |
阿拉丁生产的Small RNA Ladder (10-50nt, 9 bands)，也称microRNA Marker、miRNA Marker、小RNA ladder、小RNA Marker，是一种小RNA分子量标准，包括九条单链小RNA条带，长度分别为10、15、20、25、30', "、35、40、45和50nt，并且每种小RNA的5'和3'末端均为羟基，适合用作常规单链小RNA电泳分析检测时的分子量参照。本产品小RNA条带分布均匀细致，便于对照小RNA的大小。本产品是尿素(7M、7.5M或8M)变性聚丙烯酰胺凝胶(10%-20%)分析10-50nt小RNA时的理想选择。用NA-Red (EB升级换代产品，2000X)染色可获得非常理想的染色效果(如图1所示)。本产品中30nt条带亮度较高(参见图1)，使辨别条带更加容易。图1.用尿素(7M)变性聚丙烯酰胺凝胶(15%)电泳检测阿拉丁生产的Small RNA Ladder (10-50nt, 9 bands) 的效果图。取本产品2μl，与2μl 2X RNA Loading Buffer 混合，95℃孵育1分钟，随后迅速冰水浴冷却，然后将4μl混合物全部加入至尿素变性聚丙烯酰胺凝胶的上样孔中，180V电泳50min，之后用NA-Red (EB升级换代产品，2000X)对凝胶进行染色，在紫外灯下拍照观察结果。注意：图中30nt条带的亮度最高。上图仅供参考，实际电泳效果会因实验条件不同而有所不同。本产品配制在含50%去离子甲酰胺的DEPC水(DNase、RNase free)中。每孔上样2μl，就可以观察到非常清晰的小RNA条带(参见图1)，这样一个包装的本产品可以使用50次。 注意事项： 环境中存在较多的RNase，使用过程中容易出现RNase污染而导致本产品降解。操作过程中须严格注意避免RNase污染。宜戴口罩吸取本产品，并尽量减少Small RNA Ladder暴露于空气中的时间，取用后立即盖上盖子，尽量避免反复冻融，并建议第一次使用时对本产品适当进行分装。本产品仅用作单链RNA的分子量标准时，不建议用于精确确定小RNA长度，因为小RNA中不同核苷酸的组分也会对电泳迁移率产生一定的影响。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。 使用说明： 1.配制适当浓度的尿素(7M、7.5M或8M)变性聚丙烯酰胺凝胶，推荐使用R0218S Urea-PAGE凝胶配制试剂盒(RNA专用)。2.对于变性凝胶电泳，将2μl Small RNA Ladder与2μl 2X RNA Loading Buffer混合，在95℃孵育1分钟，迅速放至冰水浴冷却，然后将4μl混合物全部加入至尿素变性聚丙烯酰胺凝胶的上样孔中，即可电泳。3.电泳结束后，将凝胶取出放至洁净容器内(例如适当大小的玻璃培养皿)，用DEPC水清洗1-2分钟，然后加入约30ml核酸染色液，室温摇床染色15min，25rpm，最后用DEPC水洗涤2-3次，每次2-5分钟，即可使用凝胶成像设备观察电泳后的染色结果。推荐使用阿拉丁NA-Red (EB升级换代产品，2000X)进行RNA凝胶的染色，稀释时须使用DEPC水。 Aladdin's Small RNA Ladder (10-50nt, 9 bands) consists of nine single-stranded RNA oligonucleotides ranging from 10 to 50nt in length. Both the 5' and the 3' ends of each oligonucleotide are hydroxyl groups, enabling it to be used as a small RNA size marker during the conventional single-stranded small RNA electrophoresis analysis and detection.The size of these nine RNA oligonucleotides in the ladder is evenly distributed to facilitate small RNA size approximation.The ladder is an ideal choice for using 10%-20% polyacrylamide gel containing urea (7M, 7.5M, or 8M) or in 3% agarose gel to analyze small RNA molecules ranging from 10-50nt. A good staining result can be obtained by Na-red (an EB upgraded product, 2000X) (Figure 1). The 30nt band is brighter, making the ladder bands easier to be differentiated.The ladder is prepared in DEPC water containing 50% deionized formamide. This product is sufficient for 50 loadings when 2μl of ladder is loaded per well.Figure 1 Precautions： RNAase is ubiquitous in surroundings. Please take extreme care to avoid RNase contamination during the use of Small RNA Ladder. Wear a mask always during the operation. After melting, keep it on ice and make aliquots to avoid freeze-thaw cycles. Close the lid immediately after use to minimize the exposure of Small RNA Ladder to air.This product is recommended for small RNA size approximation, not for accurate size determination, because small RNA molecules may contain some compositions that affect their mobility during electrophoresis.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation. Instructions for Use： 1. Prepare urea-PAGE gel with appropriate concentrations of polyacrylamide and urea, or 3% agarose gel for electrophoresis. We recommend the Urea-PAGE Gel Preparation Kit (, R0218S) for the preparation of urea-PAGE gels.2. For denatured urea-PAGE, mix 2μl ladder and 2μl of 2X RNA Loading Buffer (, R0215) well, boil at 95℃ for 1 minute, and immediately put it on ice. Load 4μl of mixture onto a urea-PAGE gel for electrophoresis.3. For agarose gel electrophoresis, mix 2μl of ladder and 2μl of 2X RNA Loading Buffer (, R0215) well, and load the 4μl mixture onto agarose gel directly for electrophoresis.4. After electrophoresis, transfer the gel into a clean container with an appropriate size, wash the gel with DEPC water for 1-2 minutes, stain the gel with 30ml of nucleic acid staining solution for 15 minutes at room temperature on a shaker at 25rpm, wash with DEPC water 2-3 times (2-5 minutes each time), and then observe the staining result by a gel imaging system. We recommend using the NA-Red (EB upgraded product, 2000X) for RNA gel staining and using DEPC water for the dilution. |
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