阿拉丁生产的Small RNA Ladder (10-50nt, 9 bands)，也称microRNA Marker、miRNA Marker、小RNA ladder、小RNA Marker，是一种小RNA分子量标准(Small RNA Molecular Weight Marker)，包括九条单链小RNA条带，长度分别为10、15、20、25、30、35、40、45和50nt，并且每种小RNA的5'和3'末端均为羟基，适合用作常规单链小RNA电泳分析检测时的分子量参照。
本产品小RNA条带分布均匀细致，便于对照小RNA的大小。
本产品是尿素(7M、7.5M或8M)变性聚丙烯酰胺凝胶(10%-20%)分析10-50nt小RNA时的理想选择。本产品中30nt条带亮度较高，使辨别条带更加容易。
本产品配制在含50%去离子甲酰胺的DEPC水(DNase、RNase free)中。每孔上样2μl，就可以观察到非常清晰的小RNA条带，这样一个包装的本产品可以使用50次。
注意事项：
1. 环境中存在较多的RNase，使用过程中容易出现RNase污染而导致本产品降解。操作过程中须严格注意避免RNase污染。宜戴口罩吸取本产品，并尽量减少Small RNA Ladder暴露于空气中的时间，取用后立即盖上盖子，尽量避免反复冻融，并建议第一次使用时对本产品适当进行分装。
2. 本产品仅用作单链RNA的分子量标准时，不建议用于精确确定小RNA长度，因为小RNA中不同核苷酸的组分也会对电泳迁移率产生一定的影响。
3. 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
4. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. 配制适当浓度的尿素(7M、7.5M或8M)变性聚丙烯酰胺凝胶。
2. 对于变性凝胶电泳，将2μl Small RNA Ladder与2μl 2× RNA Loading Buffer (R743508) 混合，在95ºC孵育1分钟，迅速放至冰水浴冷却，然后将4μl混合物全部加入至尿素变性聚丙烯酰胺凝胶的上样孔中，即可电泳。
3. 电泳结束后，将凝胶取出放至洁净容器内(例如适当大小的玻璃培养皿)，用DEPC水清洗1-2分钟，然后加入约30ml核酸染色液，室温摇床染色15min，25rpm，最后用DEPC水洗涤2-3次，每次2-5分钟，即可使用凝胶成像设备观察电泳后的染色结果。
The Small RNA Ladder (10-50nt, 9 bands) produced by Aladdin, also known as microRNA Marker, miRNA Marker, small RNA ladder, or small RNA Marker, is a type of Small RNA Molecular Weight Marker. It contains nine single-stranded small RNA bands with lengths of 10, 15, 20, 25, 30, 35, 40, 45, and 50nt respectively. Both the 5' and 3' ends of each small RNA are hydroxyl groups, making it suitable as a molecular weight reference for routine electrophoretic analysis and detection of single-stranded small RNAs.
The small RNA bands of this product are evenly and finely distributed, facilitating the comparison of small RNA sizes.
This product is an ideal choice for analyzing 10-50nt small RNAs in urea-denatured polyacrylamide gels (10%-20%) with urea concentrations of 7M, 7.5M, or 8M. The 30nt band in this product has higher brightness, making it easier to distinguish the bands.
This product is formulated in DEPC water (DNase and RNase free) containing 50% deionized formamide. Loading 2μl per well allows for clear observation of small RNA bands, and one package of this product can be used 50 times.
Precautions：
1. There is a large amount of RNase in the environment, and this product is prone to degradation due to RNase contamination during use. Strict precautions must be taken to avoid RNase contamination during operation. It is advisable to wear a mask when pipetting this product, minimize the time that the Small RNA Ladder is exposed to the air, cover the cap immediately after use, avoid repeated freeze-thaw cycles as much as possible, and it is recommended to appropriately aliquot this product when using it for the first time.
2. When this product is only used as a molecular weight marker for single-stranded RNA, it is not recommended for accurately determining the length of small RNAs. This is because the composition of different nucleotides in small RNAs can also have a certain impact on electrophoretic mobility.
3. This product is solely for scientific research use by professional personnel. It must not be used for clinical diagnosis or treatment, nor for food or pharmaceutical purposes, and must not be stored in ordinary residential premises.
4. For your safety and health, please wear a lab coat and disposable gloves during operation.
Instructions for Use：
1. Prepare a urea-denatured polyacrylamide gel with an appropriate concentration (7M, 7.5M, or 8M).
2. For denaturing gel electrophoresis: Mix 2 μl of Small RNA Ladder with 2 μl of 2× RNA Loading Buffer (R743508), incubate at 95°C for 1 minute, immediately place in an ice-water bath to cool, then load the entire 4 μl mixture into the well of the urea-denatured polyacrylamide gel to start electrophoresis.
3. After electrophoresis, take out the gel and place it in a clean container (e.g., a glass petri dish of appropriate size). Rinse with DEPC water for 1–2 minutes, then add approximately 30 ml of nucleic acid staining solution. Stain on a shaker at room temperature for 15 minutes at 25 rpm. Finally, wash with DEPC water 2–3 times, 2–5 minutes each time. The stained electrophoresis results can then be observed using gel imaging equipment.
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