Materials Today Bio
·
2025
·
IF 10.2
Xiaobo Yu,Jingli Ding,Yingjie He,Shunbo Wei,Xing Chen,Qiujie Luo,Yuqing Zhang,Chen Qian,Jiahui Wang,Mengjie Hu,Xiang Zhang,Cuifen Lu,Jinping Liu,Jianliang Zhou
Valvular heart disease (VHD) poses a thorny problem in cardiovascular diseases. The most effective treatment for VHD is heart valve replacement. Biological heart valve (BHV) is more favored than mechanical heart valve due to the maturity of transcatheter heart valve replacement (THVR) and the absence of the need for lifelong anticoagulant use. However, traditional commercial BHV suffers degeneration within 10 to 15 years because of calcification caused by the cross-linking reagent, glutaraldehyde. Considering the remarkable properties of POSS, PEG, and the star-like eight-arm structure, we fabricated POSS-PEG-PP, which is a decellularized porcine pericardium (DPP) crosslinked by a star-like eight-arm cross-linker octafunctionalized POSS of benzaldehyde-terminated polyethylene glycol (POSS-PEG-CHO) based on the Schiff's base reaction. POSS-PEG-PP exhibits more intense fiber arrangement and better mechanical properties than GLUT-PP (glutaraldehyde crosslinked DPP). The results also show that the cytocompatibility, endothelialization, and hemocompatibility of POSS-PEG-PP are outstanding in vitro. Subsequently, in vivo assessments demonstrate that POSS-PEG-PP has anti-inflammatory and anti-calcification abilities. Furthermore, RNA sequencing analysis of subcutaneous implants suggests that the intervention of AMPK and IL-17 signaling pathways plays an important role in the inflammatory and immune responses regulation of POSS-PEG-PP. Therefore, POSS-PEG-PP is an excellent substitute material for BHVs and is expected to be clinically transformed.
研究领域:
心血管疾病
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生物材料科学
·
组织工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2019
·
IF 2.813
Bin Yang,Wenhua Li,Hui Zhao,Anling Wang,Yuanjun Lei,Qiuling Xie,Sheng Xiong
Monoclonal antibody (mAb) fragmentation is a widespread issue of protein stability that needs to be carefully monitored for critical mAb quality control during the production process development. This study describes here the discovery and characterization of CHO host cell protease-induced fragmentation of a therapeutic mAb-X in the formulation samples from an early production process. The fragmentation was observed in the sodium dodecyl sulfate capillary electrophoresis (CE-SDS) analysis of mAb-X formulation samples incubated at elevated temperature. Size exclusion liquid chromatography (SEC-HPLC) was used to analyze and collect these cleaved fragments derived from mAb-X. Reversed phase liquid chromatography mass spectrometry (RP-LC-MS) and tandem mass (MS/MS) analysis demonstrated that the fragment was generated mainly due to the hinge region cleavage of mAb-X. The fragmentation rate was characterized in the mAb-X formulation samples at pH from 4.0 to 6.0 using CE-SDS and SDS-PAGE analysis. The percentage of the main fragment increased dramatically from 2.8% to 31.2% as pH decreased from 6.0 to 4.0 at 40 °C for 28 days, which indicated the fragmentation was highly pH-dependent. The SDS-PAGE analysis further verified the pH-dependent property of the framentation of mAb-X. Moreover, the fragmentation was characterized in the presence and absence of pepstatin A, an inhibitor of acidic proteases. Significant inhibition of mAb-X fragmentation was observed with the addition of pepstatin A to mAb-X formulation samples. These results suggested residual acidic host cell protease(s) in the formulation samples from an early production process caused the fragmentation of mAb-X. To prove evidence, we developed an optimized protein A chromatography to enhance the residual host cell protease(s) removal capability of mAb-X purification process and consequently eliminate the above described cleaved fragment of mAb-X, which further supported the hypothesis that the fragmentation of mAb-X was catalyzed by the residual host cell protease(s) in the formulation samples from the early production process. This case study reiterated that residual host cell protease is a critical quality attribute (CQA) that should be carefully controlled and evaluated to guarantee successful manufacture processes for mAb products.
研究领域:
生物技术
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蛋白质工程
·
制药科学
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DOI
高影响因子
NORDIC PULP & PAPER RESEARCH JOURNAL
·
2020
·Vol. 35
·期 4
·641-648
·
IF 0.775
Xuemei Wang,Yingping Qi,Fan Wu,Yongfeng Shen,Duanhui Gao,Hua Li
Paper has a high value of culture, history and scientific research as the cultural carrier of historical inheritance. However, with the passage of time and the change of environment, many paper files are aging and damaged. Therefore, it is of great significance to carry out the protection of paper archives. Chitosan, a natural material, has a good compatibility with paper fibers. In this paper, chitosan (CS) was modified by epoxy cyclohexane (CHO) and methyl methacrylate (MMA) to prepare CS-CHO-g-PMMA emulsion. CS-CHO-g-PMMA was applied to paper protection, and the effects of CS-CHO-g-PMMA on heat aging resistance, reversibility and acid-base resistance were investigated. In order to improve the mechanical strength of paper, CS-CHO-g-PMMA and phosphate ester starch (HPDSP) were blended to determine the optimum ratio. The results showed that the degradation rate of paper sample was slowed down obviously and the coating had certain reversibility. Acid-base resistance experiment showed that CS-CHO-g-PMMA could effectively resisit corrosion of external acid-base to protect the paper. The best combination ratio between CS-CHO-g-PMMA and HPDSP was: m (CS-CHO-g-PMMA): m (HPDSP) = 5:2. Under this compound ratio, the performance indexes of the paper met the requirements, conforming to the “repair as old, keep the original” and other requirements.
研究领域:
材料科学
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文物保护科学
·
化学工程
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DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 3.7
Hongshan Li,Rongqian Mo,Jiayu Yue,Rongrong Cheng,Dianyu Li,Yusuf M. Idres,Yanmei Yang,Yanqiao Wen,Xiangrong Li,Ruofei Feng
Chinese hamster ovary cells are widely used in various biopharmaceutical industries as preferred host cells for the production of recombinant proteins. In an effort to increase yield and reduce production costs, the incorporation of small molecule additives into the culture medium has emerged as an effective approach to augment the expression of recombinant proteins, thus increasing overall efficiency. Here, we examined the impact of five small molecules on protein expression in CHO-engineered cell lines. These molecules included sodium valproate, alanyl-L-tyrosine, ascorbic acid, sodium citrate and ferrous sulphate. Their optimal concentrations were determined through univariate analysis aimed at enhancing cell growth and protein production. Our findings revealed that inoculating cells at a density of 1.0 × 10 6 cells/mL with the simultaneous addition of sodium valproate at a concentration of 2 mM for 0 h yielded the most favorable results. Notably, while valproate inhibited cell growth in a dose-dependent manner, it simultaneously extended cell culture longevity. Additionally, sodium valproate was observed to decrease glucose consumption, collectively increasing protein expression. In conclusion, our data suggest that sodium valproate is a potent chemical agent capable of increasing the production of recombinant protein in CHO cells. This enhancement is achieved by the modulation of metabolic pathways in CHO-engineered cell lines, thereby facilitating a state of low metabolism and high protein expression.
研究领域:
生物制药
·
细胞培养工程
·
分子生物学
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制药生物技术
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Jianfang Jiang,Kuaqian Deng,Fanglin Dao,Panyang Li,Jiali Huang
Trichlorfon (TCF) is an important chemical for pest and parasite control, but it poses risks to public and environmental health. Thus, the detection of TCF is of great value. In this study, we report a strategy to prepare an AChE-nanoprobe for detecting TCF. Benefiting from the unique and excellent structure of CHO-β-CD, this AChE-nanoprobe can wrap nano-Fe 3 O 4 and covalently bind AChE. As a proof of concept, we prepared an AChE-nanoprobe with a hydrated particle size of 717 nm and a saturation magnetic strength of 64.11 emu/g Fe. The properties were studied in detail. Its catalytic activity was 90 % of that of free AChE, and it had broadened pH, thermal, organic solvents and storage in addition to good reusability, with a residual catalytic activity of 42 % after the 10th cycle and a cumulative catalytic activity 6.9 times higher than that of the free enzyme. The AChE-nanoprobe exhibited good performance in TCF detection; the linear range of TCF was 40–4000 ng/L, and the limit of detection for TCF was 2.8 ng/L. Furthermore, our results suggest that the AChE-nanoprobe has several attractive features, such as easy preparation, outstanding stability and reusability, providing a novel and promising tool for the analysis of TCF.
研究领域:
环境科学
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分析化学
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纳米技术
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产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
·
IF 7.129
Liqun Huang,Shufang Li,Wenhua Zhou,Jie Gao,Jingjing Yin,Zhongwen Wang,Jianguo Li
Uranium is a radioactive heavy metal and a significant public health concern; however, its associated underlying toxicological mechanisms remain largely unknown. In this work, the uptake and efflux processes of uranium in CHO-k1 cells were studied and the cytotoxicity effects were explored. It was found that both the uptake and efflux processes took place rapidly and half of the internalized uranium was expelled within 8 h. The uranium exposure caused a decrease of cell viability and adhesion ability in a dose-dependent manner and blocked the cell cycle at the G1 stage. In addition, gene expression analysis revealed relative changes in the transcription of metabolism related genes. Further studies revealed that the cytotoxicity of uranium could be alleviated by exposing cells to a lower temperature or by the addition of amantadine-HCl, an endocytosis inhibitor. Interestingly, after uranium exposure, needle-like precipitates were observed in both intracellular and extracellular regions. These findings collectively suggest that the cellular transport of uranium is a rapid process that disturbs cell metabolism and induces cytotoxicity, and this impact could be reduced by slowing down endocytic processes.
研究领域:
毒理学
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细胞生物学
·
分子生物学
·
放射生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 13
·2971-2979
·
IF 3.286
Wang Jiaqi,Wang Chen,Fan Li,Zhao Liang,Tan Wen-Song
Chinese hamster ovary (CHO) cells are predominant in the production of therapeutic proteins to treat various diseases. Characterization and investigation of CHO cell metabolism in a quick and simple way could boost process and cell line development. Therefore, a method to simultaneously detect seven redox- and energy-related metabolites in CHO cells by capillary electrophoresis has been developed. An on-line focusing technique was applied to improve the peak shape and resolution by using a 50 μm × 44 cm uncoated fused silica capillary. Key parameters and their interactions were investigated by design of experiments (DoE) and optimized conditions were determined by desirability function as follows: 24 °C, 95 mM, and pH 9.4 of BGE. The method was validated to ensure sensitivity, linearity, and reproducibility. The limits of detection (LODs) ranged from 0.050 to 0.688 mg/L for seven metabolites, and correlation coefficients of linearity were all greater than 0.996. The relative standard deviations (RSD) of migration time and peak area were smaller than 0.872% and 5.5%, respectively, except for NADPH, and the recoveries were between 97.5 and 101.2%. The method was successfully applied to analyze the extracts from CHO cells under two different culture conditions.
Graphical abstract
研究领域:
生物技术
·
药学
·
细胞代谢
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产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2026
·
IF 18.1
Cheng Ke,Li Hao,Zhou Bing,Pei Haopeng,Hu Lufa,Zhao Long,Zou Xingyue,Wang Jiaxian,Zheng Qian,Zhao Rui,Zhang Yan,Liu Anping,Wu Zewen,Zhan Guangming,Guo Mingming,Zhang Lizhi
The electrochemical upgrading of methanol to formate is constrained by CO poisoning from *CHO intermediate dehydrogenation, hindering its industrial application. Herein, we report that engineering atomically polarized Pt δ+ -Pt δ– dipoles on Ti felt achieves a formate Faradaic efficiency of 99% at +0.9 V vs . RHE, which is better than that of commercial Pt/C (62%). Moreover, a formate production rate of 945 mmol g Pt –1 h –1 is achieved with stable performance for more than 5 days at 100 mA cm –2 . These dipoles comprise spatially adjacent electron-deficient Pt δ+ bonded to lattice O and electron-rich Pt δ– coordinated to unsaturated Ti atoms working synergistically. The Pt δ+ site dehydrogenates CH 3 OH to *CHO, which adsorbs across the dipole in a side-on Pt δ+ –OHC–Pt δ– bridging configuration. Within this configuration, Pt δ– donates electrons via d → π * backdonation to the π * antibonding orbital of *CHO, steering its direct hydroxylation and generating electrostatic repulsion for rapid HCOOH desorption. This strategy reduces environmental impact by 88% and carbon emissions by 11% relative to conventional thermal routes, demonstrating the potential of dipole engineering for C 1 electrochemistry.
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DOI
PubMed
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2020
·Vol. 67
·期 10
·1818-1826
·
IF 1.554
Yang Hu,Longchao Du,Cun Li,Jiale Shen,Weiju Zhu,Chenxi Shao
Carbon dioxide (CO 2 ) is an easily available renewable carbon source that can be used as a comonomer in the catalytic ring-opening polymerization of epoxides to form aliphatic polycarbonates. Herein, a series of new Salen-Co(III) bifunctional catalysts were synthesized for the first time, and they were studied to catalyze the copolymerization of CO 2 and propylene oxide (PO)/cyclohexene oxide (CHO). At the same time, the effects of reaction conditions (electronic effect, temperature, time) on catalytic activity and selectivity were investigated. The results show that the Salen-Co(III) complexes with electron-withdrawing groups have higher selectivity and activity for propylene carbonate (PPC)/cyclohexylene carbonate (PCHC). At the same time, the Salen-Co(III) complexes can better catalyze the copolymerization of CHO and CO 2 than that of PO and CO 2 . The catalytic efficiency of the four complexes increased with increasing temperature, and the best reaction condition is 80°C, 30 min and 2 MPa of CO 2 .
研究领域:
化学催化
·
材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Qianyi Lin,Xin Liu,Xiangji Li,Junlong Yu,Wangdu Luo,Yujie Rao,Lin Xie
Glaucoma is an irreversible blinding eye disease characterized by the loss of retinal ganglion cells (RGCs), posing a significant threat to human visual health. Currently available, clinical treatments for glaucoma cannot completely suppress the progression of RGC death in glaucoma. Eriodictyol (Eri) has been shown to possess various biological activities, including anti-inflammatory, antioxidant, and neuroprotective effects. However, its low water solubility and bioavailability hinder its clinical application. To address these challenges, we developed a ROS-responsive liposome (Lip@Eri) embedded within an F127-CHO/Gel-NH₂ hydrogel (FG/Lip@Eri) for intraocular delivery of Eri, and further evaluated its ability to suppress ferroptosis and mitigate RGC loss. This drug delivery system enhanced the bioavailability of Eri and demonstrated excellent biocompatibility. In vitro, Lip@Eri inhibited glutamate-induced ferroptosis in R28 cells by reducing iron accumulation and lipid peroxidation, associated with PI3K–AKT pathway activation. Further in vivo experiments demonstrated that FG/Lip@Eri effectively suppresses ferroptosis, protects RGCs, and preserves visual function. Therefore, FG/Lip@Eri may represent a novel strategy for neuroprotection in glaucoma.
研究领域:
眼科学
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神经科学
·
药物递送系统
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生物材料
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纳米医学
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2026
·Vol. 14
·期 27
·12026-12036
·
IF 7.6
Zhong Haojia,Liu Hongchen,Yang Fan,Sun Xinyang,Gao Fuming,Zhang Xiaona,Wei Kexin,Wang Kuobo,Li Shijia,Li Zhenxing,Wang Chunxia,Li Yongfeng
Copper-based electrocatalysts demonstrate significant potential for electrochemical CO 2 reduction toward value-added chemicals. However, achieving selective synthesis of multi-carbon (C 2+ ) products on copper-based catalysts remains a substantial challenge. In this study, a Cu 2 O electrocatalyst (Mg 0.1 -Cu 2 O-SC) doped with magnesium (Mg) and surface-modified by sodium citrate (SC) was successfully synthesized via a simple wet chemical reduction method. The Mg 0.1 -Cu 2 O-SC achieves a Faradaic efficiency (FE) of 77.8% and a partial current density of 357.9 mA cm −2 for C 2+ products, which is 1.35 times higher than the FE of unmodified Cu 2 O. Mg 0.1 -Cu 2 O achieves an enhancement in the FE for CO, demonstrating that the Lewis-acidic Mg weakens the bond strength of Cu−*CO and enhances the local concentration of *CO by attracting electrons from Cu. In situ spectroscopic analysis reveals that the surface modification of SC facilitates the *CO to *CHO hydrogenation, which shifts the C−C coupling to a more favorable *CO−*CHO route with a lower-energy barrier and consequently improves the FE for C 2+ products. Theoretical calculations indicate that the electron-withdrawing effect of Mg induces a downward shift in the copper d-band center. Energy barrier analyses reveal that SC effectively lowers the energy barriers for both the water dissociation reaction and the *CO−*CHO coupling reaction. This work confirms that Mg effectively modulates the adsorption behavior of key intermediates and synergistically optimizes the reaction pathway in concert with SC.
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DOI
高影响因子
Journal of Materials Chemistry A
·
2025
·
IF 9.5
Jie Meng,Ying Wang,Li-Yang Shao,Tian-Shu He,Jiao-Na Yue,Bo-Lin Sun,Quan-Guo Zhai
The rational control of heterostructure motifs and the corresponding electronic environment in heterocatalysts offers an effective strategy to regulate CO2RR selectivity, yet it remains challenging. The precise regulation of the reaction intermediates is therefore a critical determinant. In this work, the size-engineered cMOF/o-Cu2O was introduced to modulate heterostructure motifs, thereby modulating the *CHO intermediate selectivity switch and steering C2 or C1 reaction pathways. We elucidated the divergent CO2RR pathways governed by the differential growth degrees of the cMOF/o-Cu2O heterostructures. The minimized cMOF/o-Cu2O-s (∼361.8 nm), comprising a high proportion of –OH terminations, accelerated the continuous hydrogenation of *CHO and promoted their conversion into *CHxO intermediates. The moderate cMOF/o-Cu2O-m (∼870.1 nm) achieved an optimal balance between the intermediate concentrations and interfacial transfer, enabling a preferential *CHO–*CO coupling pathway through the rationally designed heterostructure motifs. In contrast, the enlarged cMOF/o-Cu2O-l (∼2.7 µm) exhibited an indistinguishable force toward the key intermediates of *CHO, thereby failing to differentiate between the subsequent reaction pathways. The intensified *CHxO and *CHO–*CO signals observed in the in situ ATR-FTIR further verified the bifurcated reaction pathways, featuring a C1-dominated pathway on cMOF/o-Cu2O-s and C2 pathway on cMOF/o-Cu2O-m. cMOF/o-Cu2O-m achieved a Faraday efficiency of 71.1% for C2 products at −1.3 V vs. RHE. This work offers crucial insights into the significance of size engineering in modulating the heterostructure motifs to regulate the selectivity switch of key intermediates.
研究领域:
电催化
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材料化学
·
二氧化碳还原反应（CO2RR）
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纳米材料工程
·
多相催化
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）