Life-Basel
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2022
·Vol. 12
·期 8
·1142
·
IF 3.251
Wei Wang,Jiandan Chen,Zhenzeng Zhou,Shanshan Zhan,Zhiyuan Xing,Hongying Liu,Linan Zhang
Determining the concentration of glutathione is crucial for developing workable medical diagnostic strategies. In this paper, we developed an electrochemical sensor by electrodepositing amino-based reactive groups and gold–platinum nanomaterials on the surface of glassy carbon electrode successively. The sensor was characterized by cyclic voltammetry (CV), field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDX), and electrochemical impedance spectra (EIS). Results showed that[email protected]nanoparticles with the size of 20–40 nm were presented on the surface of electrode. The sensor exhibits excellent electrocatalytic oxidation towards glutathione. Based on this, we devised an electrochemical biosensor for rapid and sensitive detection of glutathione. After optimizing experimental and operational conditions, a linear response for the concentration of GSH, in the range of 0.1–11 μmol/L, with low detection and quantification limits of 0.051 μM (S/N = 3), were obtained. The sensor also exhibits superior selectivity, reproducibility, low cost, as well as simple preparation and can be applied in human serum sample detection.Keywords:glutathione;electrochemical;sensor;[email protected]nanoparticle;ammonium carbamate modified electrode
研究领域:
分析化学
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生物医学工程
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材料科学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2021
·
IF 4.821
Lili Xiao,SiYan Zheng,Kaixiu Yang,Jiaxing Duan,Jin Jiang
Design of catalysts with unique structures in enhancing electrochemical activity and stability has still been pursued. An eco-friendly method for preparing CoP with improved electrochemical performances was proposed in this work. A novel P-doped bimetallic [email protected] ( [email protected] ) was prepared as the precursor for constructing of [email protected] /NCS, in which CoP nanospheres coated with carbon layers ( [email protected] for short) were well-dispersed on N-doped carbon spheres (NCS for short). P was strategically pre-introduced into [email protected] for preparing of [email protected] through the selective phosphorylation on ZIF-8 shell. DA was selected to investigate the electrochemical properties of [email protected] /NCS. Glassy carbon electrode (GCE) modified by [email protected] /NCS exhibited excellent properties in detection of DA by square wave stripping voltammetry (SWV), that a high sensitivity of 9.4 μA μM −1 , and low limit of detection of 0.03 μM. In addition, [email protected] /NCS showed enhanced performances on electrocatalytic oxidation of DA than that of CoP/C prepared based on ZIF-67 by the common phosphorization method reported in literatures. Moreover, a wide linear range from 5.0 μM to 400.0 μM was achieved at [email protected] /NCS/GCE by amperometric i-t. The practical application of CoP/NCS/GCE was evaluated by detection of DA in human serum. The satisfactory recoveries in diluted serum demonstrated the promising application of [email protected] /NCS.
研究领域:
材料科学
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电化学
·
纳米技术
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分析化学
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产品关联
DOI
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 12
·3082
·
IF 3.098
Wei Liu,Fan Wang,Yongchao Zhu,Xue Li,Xiaojing Liu,Jingjing Pang,Weisan Pan
Targeted drug delivery to colon cancer cells can significantly improve the efficiency of treatment. We firstly synthesized carboxyl-modified mesoporous silica nanoparticles (MSN–COOH) via two-step synthesis, and then developed calcium leucovorin (LV)-loaded carboxyl-modified mesoporous silica nanoparticles based on galactosylated chitosan (GC), which are galectin receptor-mediated materials for colon-specific drug delivery systems. Both unmodified and functionalized nanoparticles were characterized by scanning electron microscopy (SEM), transmission electron microscope (TEM), X-ray diffraction (XRD), Fourier transform infrared (FT-IR), nitrogen sorption, and dynamic light scattering (DLS). Drug release properties and drug loading capacity were determined by ultraviolet spectrophotometry (UV).[email protected]–COOH/GC had a high LV loading and a drug loading of 18.07%. In vitro, its release, mainly by diffusion, was sustained release. Cell experiments showed that in SW620 cells with the galectin receptor, the[email protected]–COOH/GC metabolized into methyl tetrahydrofolic acid (MTHF) and 5-fluorouracil (5-FU)@MSN–NH2/GC metabolized into FdUMP in vivo. MTHF and 5-fluoro-2′-deoxyuridine 5′-monophosphate (FdUMP) had combined inhibition and significantly downregulated the expression of thymidylate synthase (TS). Fluorescence microscopy and flow cytometry experiments show that MSN–COOH/GC has tumor cell targeting, which specifically recognizes and binds to the galectin receptor in tumor cells. The results show that the nano-dosing system based on GC can increase the concentrations of LV and 5-FU tumor cells and enhance their combined effect against colon cancer.Keywords:calcium leucovorin;MSN–COOH;galactosylated chitosan (GC);[email protected]–COOH/GC;galectin receptor;SW620 cells
研究领域:
药学
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纳米技术
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癌症研究
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药物递送系统
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DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2016
·
IF 2.243
Rong Tian,Xiaojun Chen,Qingwen Li,Cheng Yao
This work reports a novel signal amplification strategy based on three-dimensional ordered macroporous C 60 -poly(3,4-ethylenedioxythiophene)-1-butyl-3-methylimidazolium hexafluorophosphate (3DOM C 60 -PEDOT-[BMIm][BF 6 ]) for ultrasensitive detection of thrombin by cascade catalysis of [email protected] 2 microspheres and alcohol dehydrogenase (ADH). [email protected] 2 microspheres were constructed not only as nanocarriers to anchor the large amounts of secondary thrombin aptamers but also as nanocatalysts to catalyze the oxidation of ethanol efficiently. Significantly, the electrochemical signal was greatly enhanced based on cascade catalysis: First, ADH catalyzed the oxidation of ethanol to acetaldehyde with the concomitant generation of NADH in the presence of β-nicotinamide adenine dinucleotide hydrate (NAD + ). Then, gold nanoparticles (AuNPs) as nanocatalysts could effectively catalyze NADH to produce NAD + with the help of PEDOT as redox probe. Under the optimal conditions, the proposed aptasensor exhibits a linear range of 2 × 10 −13 to 2 × 10 −8 M with a low detection limit of 2 × 10 −14 M for thrombin detection and shows high sensitivity and good specificity.
研究领域:
分析化学
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生物化学
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纳米技术
·
电化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Zeqi Zheng,Weitao Liu,Qixing Zhou,Jiantao Li,Aurang Zeb,Qi Wang,Yuhang Lian,Ruiying Shi,Jianlin Wang
Considering the stability and economy of immobilized enzymes, this study prepared co-modified biochar immobilized laccase product named Fe 3 O 4 @ [email protected] @LC via orthogonal experimental design and explored its possibility of remediating polycyclic aromatic hydrocarbons (PAHs) contaminated soil in steel plants. Compared with the free laccase treatment, the relative activity of Fe 3 O 4 @ [email protected] @LC remained 60 % after 50 days of incubation at room temperature. The relative activity of Fe 3 O 4 @ [email protected] @LC could still retain nearly 80 % after five reuses. In the process of simulating the PAHs-contaminated site treatment experiment in Hangzhou Iron and steel plant, immobilized laccase exhibited efficient adsorption and degradation performances and even the removal rate of 5-ring PAHs reached more than 90 % in 40 days, resulting in improving urease activity and dehydrogenase in the soil and promoted the growth of a PAH degrading bacteria ( Massilia ). Our results further explained the efficient degradation effects of Fe 3 O 4 @ [email protected] @LC on PAHs, which make it a promising candidate for PAHs-contaminated soil remediation.
研究领域:
环境科学
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生物技术
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土壤学
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微生物学
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DOI
PubMed
高影响因子
TALANTA
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2020
·
IF 5.339
Qingmei Chen,Ran Gao,Li Jia
A colorimetric aptasensor was developed for selective and sensitive detection of Salmonella typhimurium ( S. typhimurium ) based on the enhancement of bacteria for the peroxidase-like activity of dual aptamers modified bovine serum albumin stabilized-gold nanoclusters ( [email protected] ). Micro-sized bacteria was found to be able to capture [email protected] and 3,3′,5,5′-tetramethylbenzidine (TMB), thus promoting the proximity of [email protected] to TMB and greatly increasing the local concentrations of the enzyme-mimetic nanoparticles and their substrate. As a result, addition of bacteria promoted the formation of blue products in the catalytic system, which was utilized to achieve bacteria detection by colorimetry . The parameters influencing the colorimetric aptasensor were optimized by an orthogonal test. Under the selected conditions, the aptasensor exhibited a wide linear response to S. typhimurium in the concentration range of 10 1 –10 6 cfu mL −1 with a detection limit as low as 1 cfu mL −1 . The feasibility of the aptasensor was verified by successful detection of S. typhimurium in egg samples with recoveries in the range of 92.4%–110%.
研究领域:
生物化学
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纳米技术
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分析化学
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微生物学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Lei Jin,Shufang Wang,Qing Shao,Yiyu Cheng
Salmonella should be absence in pharmaceutical preparations and foods according to regulations in many countries. Up to now, rapidly detecting Salmonella at 1 CFU·[10 g (mL) ] −1 in pharmaceutical preparation or 1 CFU·[25 g (mL) ] −1 in food samples is still a challenge. Herein, we present an aptamer-based surface-enhanced Raman spectroscopy (SERS) method for rapidly detecting Salmonella Enteritidis by using a handheld Raman instrument. The aptamer could specifically recognize S. Enteritidis, and 4-MBA self-assembled on the surface of [email protected] NPs was used as a Raman reporter molecule. The method was validated to be high specific with no interference from other five pathogenic bacteria. It could identify S. Enteritidis contaminant at ∼ 1 CFU·(10 g) −1 spiked level in a real sample (Wenxin granule, a botanical drug) after 6 h of enrichment. The detection time was much shorter than that of the methods (more than 54 ∼ 96 h) in the standards of pharmaceutical preparations and foods. In addition, the method could quantitatively determinate S. Enteritidis with satisfactory results. The SERS peak intensities of 4-MBA at 1072 cm −1 showed a good linear correlation (R 2 = 0.9873) with the logarithms of S. Enteritidis concentrations ranging from 4.17 × 10 2 to 1.39 × 10 7 CFU·mL −1 . T-test result (P = 0.425) revealed that there was no significant difference between the determination results obtained by the SERS method and the plate counting method. Therefore, the study indicated that the method was practical and reliable, and it could be a promising alternative for the on-site detection of S. Enteritidis.
研究领域:
分析化学
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食品科学
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药学
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微生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Wuzhen Qi,Lingyan Zheng,Yu Hou,Hong Duan,Lei Wang,Siyuan Wang,Yuanjie Liu,Yanbin Li,Ming Liao,Jianhan Lin
A microfluidic biosensor was developed for rapid detection of Salmonella using a finger-actuated micropump, a finger-actuated micromixer, [email protected] nanocatalysts ( [email protected] ) and a smartphone App. First, immune magnetic nanobeads (MNBs), bacterial sample and immune [email protected] were successively finger-actuated pumped into microfluidic chip. Then, they were fully mixed using finger-actuated micromixer to form MNB- Salmonella [email protected] complexes. After hydrogen peroxide-tetramethylbenzidine was pumped into chip and catalyzed by nanocatalysts on complexes, resulting in color change from colorless to blue, the image of catalysate was collected and finally analyzed by self-developed smartphone App or directly compared with Pantone color card in chip to determine bacterial concentration. Experimental results showed this biosensor could quantitatively detect Salmonella from 3.5 × 10 2 to 3.5 × 10 5 CFU/mL in 1 h with lower detection limit of 350 CFU/mL. This biosensor has successfully integrated loading, mixing, incubation, washing, separation and detection onto a chip and might pave a promising way for bacterial detection.
研究领域:
微流控技术
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生物传感器
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食品安全
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病原体检测
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纳米技术
·
即时诊断
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产品关联
DOI
PubMed
高影响因子
Energy Storage Materials
·
2019
·
IF 0
Biao Liu,Minhao Yang,Wen-Ying Zhou,Hui-Wu Cai,Shao-Long Zhong,Ming-Sheng Zheng,Zhi-Min Dang
Film capacitor, one typical type of electrostatic capacitors, exhibits its unique advantages in the high-power energy storage devices operating at a high electric field due to the high electrical breakdown strength ( E b ) of the polymeric films . However, the development of film capacitor towards high energy storage density is severely hindered by the low dielectric constant ( ε ) and low charge-discharge efficiency ( η ) of the polymeric films. The film of polypropylene (PP), the most used polymeric film with a market share of 50%, owns a high η due to its low inherent hysteresis loss . Yet the low ε (2.2 at 10 3 Hz) impedes the increase of its energy storage density (1–2 J/cm 3 ). Here we demonstrate that the discharged energy density ( U e ) of PP film could be largely increased from 1.40 J/cm 3 of pure PP film to 3.86 J/cm 3 of PP nanocomposite film by incorporating a small loading of core-shell structured [email protected] 3 ( [email protected] ) nanoparticles (2.27 vol%) into PP matrix. The obtained U e of 3.86 J/cm 3 , to the best of our knowledge, is the highest reported value for the PP based films using commercial PP resin. Meanwhile, the η merely undergoes a slight decrease from 99.5% to 94.1%. Similarly, the obtained η of 94.1%, to the best of our knowledge, is also the highest value for the polymeric films reported in the previous works. The significant increase of U e (175.7%) and negligible decrease of η (5.4%) are mainly attributed to the increases of ε from 2.2 to 3.7 and E b from 361 MV/m to 448 MV/m. Compared with the films of raw BT/PP nanocomposites, the U e exhibits a significant increase of 365.1% from 0.83 J/cm 3 to 3.86 J/cm 3 and the η also displays an increase of 7.4% from 87.6% to 94.1%. The significant improvement of energy storage performance achieved in the film of [email protected] /PP nanocomposite suggests that the organic PMMA shell plays an important role in the amelioration of compatibility of BT nanoparticles and PP matrix and the alleviation of local electric field concentration. Moreover, the PMMA shell could also provide a robust scaffold to hinder the early breakdown failure of nanocomposites due to its high E b (425 MV/m). Thus, high ε , low loss and high E b are simultaneously achieved. Specifically, the high hot stretch ratio (1:4) of nanocomposite film indicates the strong feasibility of industrialized film processing. Apart from the experimental analysis, theoretical analysis using the simulation of finite element is also carried out to figure out the influence of organic PMMA shell on the dielectric performance of PP nanocomposites films. These findings enable the development of film capacitor using non-polar PP based film towards high energy storage density.
研究领域:
材料科学
·
电气工程
·
纳米技术
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产品关联
DOI
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Weichao Xu,Yuxuan Yi,Kunhao Guo,Anming Li,Xuehan Zhang,Feng Guo
The combination of photocatalysis and enzyme catalysis makes many previously difficult or slow chemical reactions easy to realize. A novel [email protected] −C 3 N 4 biohybrid catalyst was successfully synthesized using a simple in situ synthesis method to remove 4- tert -butylphenol (4- t -BP). A series of characterization methods were used to investigate the structure, morphology, chemistry, and optical properties of biohybrid catalysts. [email protected] −C 3 N 4 has improved photogenerated electron-hole pair separation efficiency and charge carrier mobility , as well as photocatalytic activity, with a removal rate of 4- t -BP nearly 100% within 180 min. The catalyst recycling test revealed that the composite could maintain its high removal efficiency after 10 recyclings. Meanwhile, the photocatalytic mechanism investigation reveals that ∙O 2 − play critical roles in the removal of 4- t- BP by g−C 3 N 4 . A detailed examination of the mechanism and pathway of 4- t -BP removal by photoenzyme oxidation was also carried out. This biohybrid catalyst opened up a new avenue for the advancement of environmental protection technology .
研究领域:
环境科学
·
化学工程
·
材料科学
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催化
·
环境技术
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yingxia Zhou,Lai Wei,Lihua Chen
Herein, a small molecule, hemin/G-quadruplex (G4-hemin), was physically embedded into NH 2 -MIL-53 (Al) to construct a novel metal-organic framework (MOF)-based enzyme ( [email protected] ). Its catalytic activity and stability were investigated in detail by catalyzing a reaction of 3,3′,5,5′-Tetramethylbenzidine (TMB) in the presence of H 2 O 2 . The results show that the activity and stability of this new enzyme is better than that of traditional hemin or G4-hemin, and even stronger than that of [email protected] ( [email protected] > G4-hemin > [email protected] > hemin). No significant loss in catalytic activity was observed for at least one day. Even after eight days, only a 13.8 % decrease in activity was recorded. An electrochemical biosensor was successfully developed based on a signal amplification strategy utilizing the catalysis of [email protected] The sensor was able to sensitively detect TB in buffer, even in a diluted solution of many proteins. The proposed method offers high selectivity against mismatch sequences of TB, a good linear response range of 10 −18 moL L -1 to 10 -14 moL L -1 and a low minimum detection limit of 0.330 aM.
研究领域:
生物化学
·
分析化学
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材料科学
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生物医学工程
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Lei Wang,Ai Xu,Jing Yuan,Fan Jiang,Meixuan Li,Wuzhen Qi,Yanbin Li,Jianhan Lin
An hourglass-mimicking biosensor was developed to detect target bacteria in 15 mL centrifugal tube using immune magnetic nanobeads to isolate the target from large-volume sample, [email protected] nanozymes to label the target for amplification of biological signals, and a microplate reader/colorimetric card for determination of the target. First, a centrifugal tube with an iron ball framework was first coaxially assembled in the center of a Halbach ring magnet. After immune magnetic nanobeads, [email protected] nanozymes and bacterial sample were mixed by repeated bottom-up of the tube using a stepper motor , nanobead-bacteria-nanozyme complexes were formed. Then, colorless H 2 O 2 -TMB was catalyzed by the nanozymes to produce blue TMBox. The color change was finally analyzed using the microplate reader or colorimetric card to determine bacterial concentration . This hourglass-mimicking biosensor could separate ∼95% targets from 10 mL bacteria sample and detect targets from 1.6 × 10 1 to 1.6 × 10 6 CFU/mL in 1.0 h with low detection limit of 16 CFU/mL.
研究领域:
生物技术
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生物传感器
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微生物学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）