iScience
·
2022
·
IF 6.107
Wei Shi,Chunhui Li,Haiqing Wang,Zhiquan Wang,Lei Yang
Owing to the characteristics of THz wave, the terahertz time-domain spectral (THz-TDS) system has potentials in the field of biological macromolecule detection. However, water with strong absorption effect on THz wave exists in most biological detection, so the research focus in this field is to study aqueous samples. In view of these, THz spectroscopy system has research value for qualitative and quantitative detection of α-lactose and its water-containing samples. This research used a THz-TDS system with LiNbO 3 crystal to generate strong THz wave that was used to test 0.29 mmol α-lactose samples with water content of 15 μL–930 μL by using attenuating total reflection (ATR) prism. The absorption peak at 0.53 THz is detected, and with the increase of water content, the curve of absorption spectrum is observed to move up on the whole. This research has a guiding role for the test and improvement of water content limit in this field.
研究领域:
生物物理学
·
分析化学
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DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Yongmei Wang,Zongshan Zhao,Jianyuan Qin,Huan Liu,Aifeng Liu,Mengmeng Xu
A simple, green and nondestructive method based on terahertz fingerprint peaks has been developed for rapid in situ analysis of l -histidine and α-lactose in dietary supplements. Fingerprint absorption peaks of l -histidine and α-lactose located at 0.77 and 0.53 THz could be directly used for identification and quantitation of these analytes in commercial dietary supplements. Compared with the partial least squares regression model (PLSR), the linear least squares regression (LLSR) method based on peak areas presented better performance, with the linear correlation coefficients of 0.9899 and 0.9910 for l -histidine and α-lactose, respectively. Furthermore, analysis time per sample can be shortened to less than 1 min due to the narrower spectral acquisition region. The accuracies were 94.8–110% and 98.9–110%, comparable to those of ion chromatography for l -histidine and high-performance liquid chromatography for α-lactose. The results presented great potential of the developed method for rapid in situ analysis of nutrients in dietary supplements.
研究领域:
分析化学
·
营养学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING RESEARCH & DESIGN
·
2026
·
IF 3.9
Liang Zhang,Haifeng Lu,Xiaolei Guo,Haifeng Liu
This study addresses the challenges of micro-dosing and mixing in the continuous manufacturing of solid pharmaceutical formulations by developing a novel continuous feeding-mixing system based on external vibration excitation. The innovative design of a hopper structure with adjustable outlet area distribution demonstrated the potential for achieving precise mixing control of an active pharmaceutical ingredient (API) and an excipient across varying ratios. Experiments first validated the promotion of viscous particle flow by vertical vibration, achieving stable feeding within the range of 0.6–15 mg/s. Building on this, precise control of mixing ratios was enabled by designing different outlet area ratios. In mixing performance evaluation, online X-ray fluorescence spectroscopy confirmed the system achieves high-uniformity mixing with a coefficient of variation below 10 %. The study further revealed that optimal mixing stability occurs when Γ < 5, whereas excessive vibration degrades mixing quality.
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高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Mengyue Gao,Zhiheng Li,Mengjie Liu,Ting He,Xiaolu Su,Yueting Zhao,Zhiqiao Zou,Yanxing Yao,Fengmei Jin,Zhihao Li,Lihua Wang
A dual-metal-site MOF nanozyme exhibits enhanced oxidoreductases-like activity. • DMS MOF nanozyme-based sensor shows high sensitivity in Cr(VI) detection. • The sensor shows excellent performance in dairy product samples. A dual-metal-site metal-organic framework (DMS MOF) nanozyme was developed for highly sensitive colorimetric detection of Cr(VI) in dairy products. DMS MOF nanozyme integrates trivalent iron nodes and iron-porphyrin ligands to mimic the multi-center natural oxidoreductases, with adjacent dual-metal centers synergistically enhancing catalytic activity. Steady-state kinetic analyses and Zeta potential investigations demonstrate that the DMS MOF exhibits significantly lower Michaelis-Menten constants and enhanced substrate affinity than single-metal-site MOFs. The proposed method based on DMS MOF nanozyme enables highly sensitive colorimetric sensing of Cr(VI) within merely 5 min, achieving a broad linear range from 0.50 ppb to 5.0 ppm and a limit of detection (LOD) of 0.36 ppb. Furthermore, the developed strategy has been validated in complex dairy products including pure milk, yogurt, and milk powder, yielding recoveries of 84.0–110% with RSD ≤2.10%. Thus, engineered DMS-MOF nanozymes with enhanced activity provide a cost-effective, reliable approach for heavy-metal detection in foods. Download: Download high-res image (173KB) Download: Download full-size image
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DOI
PubMed
高影响因子
Separation Science Plus
·
2026
·Vol. 9
·期 3
·e70209
·
IF 2.7
Lianfeng Liu,Turghun Muhammad,Jia Zhao,Feiyu Zhan,Yan Zhang
To address the poor reproducibility associated with manual acceptor phase collection in two-phase hollow-fiber liquid-phase microextraction, we introduce a novel centrifugal collection technique for the first time. Thiamine, selected as a model analyte, was oxidized into fluorescent thiochrome and extracted via hollow-fiber liquid-phase microextraction. Following extraction, centrifugation at 7000 rpm quantitatively transferred 15.7 ± 0.4 µL (relative standard deviation [RSD] 2.55%) of the acceptor phase, accounting for over 80% recovery, from the hollow fiber into a glass capillary for direct fluorescence detection. This procedure enhanced collection efficiency nearly 7-fold and reduced RSD to 2.55%, compared with 2.3 ± 0.5 µL and 21.74% for the conventional syringe-based method. Optimal extraction conditions were established as follows: n -heptanol as the acceptor phase, a 20 min extraction time, 400 rpm orbital shaking, 15% NaCl, and 8 min for acceptor phase loading. Under these optimal conditions, a good linear relationship was established for thiamine across the concentration range of 0.05–3.0 µg/mL ( R 2 = 0.9996), with recoveries exceeding 91.0% and a limit of detection (LOD) of 1.112 × 10 −2 µg/mL. The intra-day and inter-day RSD ( n = 7) were below 1.7%. The proposed centrifugal collection method is simple, highly reproducible, and efficient, offering a generic strategy to enhance reliability and throughput for the hollow-fiber liquid-phase microextraction technique.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
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IF 4.8
Junnan Wang,Lei Hou,Yusong Zhang,Lei Yang,Wei Shi
In recent years, although quantitative, nondestructive terahertz (THz) testing has developed rapidly, a broadband and fast approach for material quantification is still lacking. In this paper, a Rydberg-atom THz detector is applied to measure the concentration of α -lactose monohydrate ( α -LM). Specifically, when cesium atoms are excited to the 40D 5 / 2 state by two lasers, the probe transmission exhibits an electromagnetically induced transparency (EIT) signal, and then splits into an Autler–Townes doublet in the presence of a THz field, with the splitting interval proportional to the THz field strength. Meanwhile, α -LM attenuates the THz field incident on the cesium atom vapor, which reduces the AT splitting, therefore, the sample concentration can be inferred by the AT splitting interval and concentration relationship. Moreover, the same samples were measured by a conventional THz time-domain spectroscopy (THz-TDS) system for comparison. While the THz-TDS system failed to establish a reliable calibration at non-resonant frequencies, the Rydberg-atom detector achieved excellent agreement with the Beer–Lambert law across a wide range of concentrations, even at a non-resonant frequency of 108.9 GHz. Consequently, this approach has no requirement for the sample ′ s characteristic absorption peaks and the system ′ s bandwidth. The proposed method proves Rydberg-atom sensor is a powerful and versatile tool for quantitative THz spectroscopy, and pave a way for non-destructive testing and material analysis.
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高影响因子
OPTICS AND LASERS IN ENGINEERING
·
2025
·
IF 3.7
Binggang Xiao,Yichun Wang,Xiuran Zuo,Luqi Liu,Jianyuan Qin,Wanshun Jiang,Yumei Zhang,Lihua Xiao
This study presents a dual-band terahertz metasurface biosensor based on the electromagnetically induced transparency (EIT) effect. The sensor employs copper resonant rings and a quartz dielectric substrate, enabling switching between single and dual-EIT (D-EIT) modes by altering the polarization direction of incident terahertz waves. The physical mechanism of the dual-EIT effect was elucidated through electric field analysis, revealing bright-dark mode coupling (0.53 THz) and bright-bright mode coupling (0.76 THz). Simulations demonstrated a refractive index sensitivity of up to 220 GHz/RIU. Experimentally, the metasurfacel fabricated via laser engraving exhibited dual-frequency sensing capabilities for lactose and chlortetracycline hydrochloride, with a minimum detectable concentration of 0.1 mg/L in both aqueous and milk solutions. Despite limitations in low-concentration detection within complex media due to increased optical loss, this sensor shows significant potential for applications in food safety and antibiotic residue monitoring.
研究领域:
传感器
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光学
·
生物物理学
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纳米技术
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分析化学
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食品安全
·
生物医学工程
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DOI
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Xiaokang Tian,Rongrong Fan,Wenyi Wei,Dan Wang,Yuan Le,Jian-Feng Chen
Low efficacy and frequent use of conventional pesticide formulations have led to various food safety and ecological concerns. Green and effective nanoformulation offers promising solutions to water-insoluble pesticides. Herein, we prepared a water-based nanoparticulate formulation of lambda-cyhalothrin (LC WBNF) using high-gravity rotating packed bed (RPB) combined with lyophilization. The prepared formulation could be dispersed in water with an average particle size of 54.5 ± 0.7 nm and maintained long-term stability. LC WBNF demonstrated significant improvement in foliar wettability, adhesion, and resistance to rain-induced washout. LC WBNF enhanced the uptake and translocation of LC in broad bean plants due to the presence of nanoparticles. Furthermore, the insecticidal activity of LC WBNF was 2.8 and 2.3 times higher than that of commercial LC WP and LC WDG, respectively. This study presents a novel strategy for the development of nanoinsecticides for practical application in agricultural production.
研究领域:
农业化学
·
纳米技术
·
农药科学
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环境毒理学
·
植物保护
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Fang Wang,Yi-Mei Li,Jing Zhao,Jing Ye,Yuan-Ting Li
This study presents a dual-mode colorimetric and surface enhanced Raman scattering (SERS) platform for the highly sensitive and selective assay of N-nitrosodiethylamine (NDEA) in antihypertensive drugs. A polydopamine embedded molecularly-imprinted agarose hydrogel (MIAG) chip was developed, serving as a multifunctional sensing substrate. Upon exposure to NDEA, the chip facilitates specific target adsorption and enrichment, simultaneously triggering a Griess reaction that yields a deep-red azo compound. This reaction provides the basis for both colorimetric readout and generates a strong Raman signal through azo-enhanced scattering for quantification. This design allows the MIAG chip to function not only as a naked-eye indicator but also as a platform for smartphone-based colorimetric analysis by directly translating color responses into digital barcodes. Furthermore, the in-situ synthesized gold nanoparticles (AuNPs) within the hydrogel matrix generate enhanced SERS activity, leveraging the porous network to create abundant hotspots for ultra-sensitive detection, achieving a limit of detection as low as 2.3 × 10 −14 M. The synergistic combination of dual-mode output offers robust and cross-verification results, significantly improving detection reliability. This integrated smartphone-compatible platform holds great promise for point-of-care testing of drug safety, making accurate screening accessible from regulatory laboratories to the public
研究领域:
分析化学
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生物传感器
·
纳米技术
·
药物分析
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材料科学
·
生物医学工程
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DOI
高影响因子
APPLIED CLAY SCIENCE
·
2024
·
IF 5.3
Ping Chen,Hui Zhong,Xiao-rong Li,Tingting Zhang,Xiang Wang,Fang Hu,Yubo Yan,Jiaxing Xu,Meisheng Li
A novel nanozyme, based on copper-ion-complexed palygorskite (Pal Cu 2+ ), was synthesized using a facile method. The introduction of Cu 2+ demonstrates a synergistic effect with Pal, enhancing the peroxidase-like activity for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 . Steady-state kinetic analysis revealed that Pal Cu 2+ exhibits a higher affinity for substrates compared to native horseradish peroxidase, presenting a practical alternative to natural peroxidase. The efficacy of Pal Cu 2+ as a peroxidase mimic for H 2 O 2 sensing was investigated through colorimetric methods. Additionally, when combined with enzymatic glucose oxidation, a sensitive and selective colorimetric strategy for glucose detection was established. The developed method was effectively applied to detect H 2 O 2 and glucose in real samples. The proposed strategy offers an approach for the rational design and development of metal ion composites, opening new way for future applications of Pal-based materials.
研究领域:
生物化学
·
分析化学
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DOI
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Changmao Yang,Yue Xiao,Yunjun Yan,Houjin Zhang
The development of straightforward and cost-efficient methods for bacterial identification is very important. In this study, we utilized xylenol orange, metal ions, and diverse bacterial carbon sources to construct a sensor array, achieving precise bacterial identification. Initially, we examined the absorbance variations of xylenol orange with five metal ions (Mn2+, Co2+, Ni2+, Cu2+, and Zn2+) at pH levels ranging from 4 to 7, observing significant changes at 570 nm or 580 nm. Given that bacteria can generate varying amounts of acid in different carbon sources, we employed a blend of xylenol orange and the five metal ions as pH-sensitive probes to characterize bacterial metabolism in three carbon sources, resulting in the development of a five-sensor array that effectively differentiated seven bacteria. Additionally, by utilizing xylenol orange with Co2+, we successfully identified six different bacterial mixtures and five types of probiotic drink products. These findings highlight the potential of our methods for broader practical application in bacterial identification in practical use.
研究领域:
微生物学
·
生物传感技术
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Wei Cheng,Huilin Wen,Haixia Shen,Chenwei Fu,Erkuang Miao,Dongbao Li,Xiaoqiang Chen,Chi Hu,Ziyi Yu
Polymer hydrogels are instrumental in cell immobilization for biomanufacturing, yet traditional hydrogel bioreactors often compromise cell viability, function, and mass transfer. This results in uncontrolled microbial proliferation and significant cell leakage, thereby impeding the continuous production of high-value products. To address these challenges, we introduce an innovative liquid microcavity technique employing a blend of Gelatin-methacryloyl (Gel-MA) and Pluronic F127diacrylate (F127-DA) for precision bioprinting, followed by selective enzymatic digestion of gelatin to create microbial habitats. Through tailoring of the hydrogel’s pore structure and integration of diverse networks, our approach facilitates controlled nutrient transfer, efficient waste removal, and stable mechanical support, all while maintaining a leak-free suspension culture of microorganisms. We validated the effectiveness of our method by fabricating yeast-infused Three-dimensional (3D) lattice constructs, successfully achieving continuous fermentation of glucose into ethanol in a flow reactor with minimal efficiency reduction (≥90 % yield) over five cycles. This research not only confronts the spatial limitations inherent in hydrogel-based systems but also demonstrates a foundational potential for revolutionary change in biomanufacturing practices.
研究领域:
生物技术
·
材料科学
·
生物工程
·
微生物学
·
化学工程
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DOI
高影响因子
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