COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2019
·
IF 3.131
Xing-An Luo,Pei Zhao,Hai Zhang,Shu-Yi Feng,Ke-Xian Chen,Zhong-Xiu Chen
Dietary macromolecules are often used for food formulation. Most of the published literature usually focuses on a certain polysaccharide reducing lipid digestion, but the non-specific physical effects such as the excluded volume effect of the surrounding macromolecules, which will possibly enhance the hydrolysis of lipids, is rarely discussed. As the influence of polysaccharides on the gastrointestinal fate of α-tocopherol acetate (VE-A) encapsulated in emulsion have not been conducted so far, in this research, we study the effect of crowded medium created by polysaccharides (Ficoll400) on the hydrolysis of VE-A and its bioaccessibility. The results show that the presence of Ficoll400 increased the bioaccessibility and conversion of VE-A 1.6 times that of a dilute buffer. An enzyme-catalyzed kinetic reaction rate of VE-A hydrolysis is increased up to 3.5 times. Both the improved thermodynamic conversion yields and kinetic rates of the hydrolysis of the VE-A emulsion was further observed in a synthetic macromolecule PEG2000 medium. This research improves our understanding of the crowding effect of macromolecules on VE-A/lipid emulsion digestion.
研究领域:
食品科学
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营养学
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生物化学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Hailu Liao,Chenlinrui Yue,Zhenni Zhai,Xiaoni Lian,Hongkai Xie,Yong Fang,Fengjiao Fan
In this study, three-layer films based on gelatin/gelatin-dextran/gelatin with different concentrations of functional fillers (cinnamaldehyde and α-tocopherol) were fabricated by using the layer-by-layer casting method to preserve refrigerated scallop adductor muscle. The films demonstrated excellent bonding, with functional fillers creating a porous cross-section, a slightly rough surface, and increased hydrophobicity while maintaining thermal stability. The crystallinity, water contact angle, thickness, opacity, antioxidant activity, and antibacterial activity of the films were increased, while the water vapor permeability and mechanical properties were decreased with increasing concentrations of the functional fillers. The film with 1.2% active fillers showed over 82% free radical scavenging activity and significant inhibition zones (24.7 ± 1.2 to 43.9 ± 1.8 mm) against five foodborne pathogens and spoilage bacteria. Both cinnamaldehyde and α-tocopherol demonstrated controlled release properties in four food simulants, with the maximum release and minimum release times observed in 95% ethanol (9.0 h) and distilled water (2.0 h), respectively. Overall, the introduction of 1.2% of active fillers into the films significantly enhanced the chemical and microbiological stability of refrigerated scallops and prolonged their shelf life by 6 days. Furthermore, the developed films showed excellent controlled release, antibacterial, and antioxidant properties, making them potential candidates for preserving seafood.
CAS / MDL:
研究领域:
食品科学
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材料科学
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海洋生物学
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产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2017
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IF 4.3
Yuling Liu,Sai Fu,Longfei Lin,Yuhong Cao,Xi Xie,Hua Yu,Meiwan Chen,Hui Li
Pluronic F127 (F127), an amphiphilic triblock copolymer, has been shown to have significant potential for drug delivery, as it is able to incorporate hydrophobic drugs and self-assemble into nanosize micelles. However, it suffers from dissociation upon dilution owing to the relatively high critical micelle concentration and lack of stimuli-responsive behavior. Here, we synthesized the α-tocopherol (TOC) modified F127 polymer (F127-SS-TOC) via a redox-sensitive disulfide bond between F127 and TOC, which formed stable micelles at relatively low critical micelle concentration and was sensitive to the intracellular redox environment. The particle size and zeta potential of the F127-SS-TOC micelles were 51.87±6.39 nm and -8.43±2.27 mV, respectively, and little changes in both particle size and zeta potential were observed within 7 days at room temperature. With 10 mM dithiothreitol stimulation, the F127-SS-TOC micelles rapidly dissociated followed by a significant change in size, which demonstrated a high reduction sensitivity of the micelles. In addition, the micelles showed a high hemocompatibility even at a high micelle concentration (1,000 μg/mL). Low cytotoxicity of the F127-SS-TOC micelles at concentrations ranging from 12.5 μg/mL to 200 μg/mL was also found on both Bel 7402 and L02 cells. Overall, our results demonstrated F127-SS-TOC micelles as a stable and safe aqueous formulation with a considerable potential for drug delivery.
研究领域:
药剂学
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生物材料学
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产品关联
DOI
PubMed
高影响因子
ACTA PHYSIOLOGIAE PLANTARUM
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2023
·Vol. 46
·期 2
·1-17
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IF 2.6
Kumari Monika,Singh Hidam Bishworjit,Khan Mohammad Imtiyaj
Betalains are health-promoting plant pigments accumulated in non-anthocyanic plants. Owing to its limited distribution in nature, metabolic trade-offs of inhibiting them are poorly understood. The aim of this study was to investigate the effects of inhibition of betalains in Amaranthus tricolor seedlings to gain insights into the relationship of betalain biosynthesis with other biosynthetic pathways in betalain-accumulating plants. Phenylalanine (Phe; 12.5, 25, and 50 mmol L −1 ) and 3-methyl-2-benzothiazolinone hydrazone (MBTH) (25 mmol L −1 ) were treated to inhibit betalain biosynthesis in ten-day-old A. tricolor (red) seedlings. After two and six days of treatment, target (betalain-related metabolites) and non-target metabolites were analyzed. In two days, Phe content increased by 2.6, 8.5, and 17.4-fold in Phe (12.5, 25, and 50 mmol L −1 )-treated seedlings, respectively, compared to control, indicating the uptake of Phe by the seedlings. Phe treatment led to a 10.1–18% decrease in betacyanins, while MBTH caused an 18.3% decrease in two days. In both treatments, cyclo -DOPA formation, which is essential for betacyanin biosynthesis, seems to be inhibited, albeit through different mechanisms. Betalain biosynthetic precursors and intermediates, viz., tyrosine, L-DOPA, and dopamine decreased differentially. Ascorbic acid, α -tocopherol, and retinol contents increased in both treatments concomitant with the reduction in betalains, total phenols, and antioxidant enzymes. Therefore, Phe treatment is beneficial in enhancing antioxidant metabolites in betalain-accumulating plants. However, the mechanism of increasing ascorbic acid on inhibiting betalains needs further investigation in other betalain-producing plants also to understand if ascorbic acid is involved in regulating betalain biosynthesis.
研究领域:
植物学
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代谢生物学
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产品关联
DOI
高影响因子
Antioxidants
·
2023
·Vol. 12
·期 3
·564
·
IF 7
Lingling Ma,Tiecheng Gao,Hao Cheng,Ning Li,Weining Huang,Li Liang
Protein particles have been reported as the potential carriers for the co-encapsulation of bioactive components. In this study, lysozyme, a basic protein, was used to simultaneously encapsulate folic acid andα-tocopherol at pH 4.0. The encapsulation efficiency and loading capacity of folic acid orα-tocopherol increased with its respective concentration. Folic acid had no influence on the encapsulation ofα-tocopherol. However, the encapsulation of folic acid was improved byα-tocopherol below 40 μg/mL but reduced byα-tocopherol at higher concentrations. The encapsulation by lysozyme shielded folic acid,α-tocopherol, or both partially from the attack of 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical cation. No masking effect of lysozyme encapsulation onα-tocopherol was found in DPPH antioxidant activity assay. Furthermore, the DNA coating was used to improve the dispersion of lysozyme with folic acid andα-tocopherol. The lysozyme/DNA particles with folic acid andα-tocopherol showed a homogenous size distribution of 180–220 nm with ζ-potential values between −33 and −36 mV. The release and bioaccessibility of folic acid in lysozyme/DNA withα-tocopherol were similar to that of folic acid alone, while the release ofα-tocopherol was delayed and its bioaccessibility was improved by encapsulation in lysozyme/DNA with folic acid. The data gathered here would provide guidance for the use of lysozyme-based co-encapsulating carriers in the development of functional foods.Keywords:lysozyme;folic acid;α-tocopherol;DNA coating;encapsulation;bioaccessibility
研究领域:
食品科学
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营养学
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生物技术
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Lei He,Yongze Liu,Haomiao Zhu,Feng Chen,Jiong Shi,Panpan Song,Fuqi Feng,Juqing Cui,Xiaofeng Ma,Jun Zhang
Nasal diseases are pervasive infections that can impair the quality of life of sufferers. In this study, a flexible modified triplochitin scleroxylon (MTS)-based Janus hydrogel (MTS@P/DLT) was obtained by disrupting the homogeneity of the system using DL-α-tocopherol (DLT) with flexible wood as the framework and poly(vinyl alcohol) (PVA) as the outer matrix. At a PVA concentration of 10 wt %, DLT formed a homogeneous layer, as revealed by electron microscopy. Mechanical property analysis showed that chemical and hydrogel modifications significantly increased the flexibility and toughness of the hydrogel. Microscopic and macroscopic assessment of the characteristics of the two sides of the MTS@P/DLT hydrogel revealed that one side of the DLT film had antiadhesion effects. Molecular dynamics simulation was used to determine the movement law of DLT in a PVA solution. The stronger intermolecular force between DLT and PVA than that between DLT and water, as well as between DLT molecules, enabled the formation of a homogeneous DLT film by controlling the concentration of PVA. In vitro and in vivo models demonstrated the potential of MTS@P/DLT to resist infection and facilitate recovery. Thus, MTS@P/DLT is promising for medical use.
研究领域:
材料科学
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医学
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生物工程
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2018
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IF 3.129
Kaiqiang Wang,Da-Wen Sun,Qingyi Wei,Hongbin Pu
Emulsion is a commonly investigated bioactive loaded delivery system. The bioactive content and its location in oil phase primarily determine the quality and chemical stability of emulsion. In this study, Raman microspectroscopy was used to quantify α-tocopherol and to visualize its distribution in oil-in-water emulsion stabilized by whey protein isolates. Results suggested that α-tocopherol contents (25–300 g/kg) in corn oil with the integrating Raman intensity at 481.9 and 588.6 cm −1 showed determination coefficient ( R 2 ) of 0.98 and 0.99, and limit of detection of 5.1 and 21.2 g/kg, respectively. For detecting α-tocopherol in emulsions, the relative standard deviation values from Raman method using intensities at 481.9 cm −1 and 588.6 cm −1 were in the ranges of 4%–16% and 2%–6%, respectively. The developed Raman method provided correlative results with those of HPLC method ( R 2 = 0.99). Moreover, Raman chemical imaging depicted the non-homogeneous TOC distribution within oil droplets, where TOC had the trend of migrating to the interface of oil and water. This study provided a novel approach for functional emulsion analysis, which may serve the basis for designing stable and controllable release of emulsion systems in future.
研究领域:
食品科学
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分析化学
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产品关联
DOI
高影响因子
JOURNAL OF COORDINATION CHEMISTRY
·
2025
·
IF 2.1
Mingzhe Li,Yuyang Zhou,Hua Zhou,Dingqiang Lu
Vitamin E is an important fat soluble antioxidant, its acetate is the main derivative product of vitamin E. Using metal organic framework material ZIF-8 as a catalyst, vitamin E acetate (VEA) was prepared by catalyzing the esterification reaction of D-α-tocopherol and acetic anhydride in a solvent-free system. Firstly, the preparation conditions of metal organic framework materials were studied: different imidazole ligands, different zinc ion ligands, molar concentration ratio of Zn2+ to 2-methylimidazole(2-mIM), and preparation time. The results showed that the catalytic performance of ZIF-8 was optimal when Zn(CH3COO)2·2H2O was used as the zinc source, 2-mIM was used as the imidazole ligand, the molar concentration ratio of Zn2+ / 2-mIM was 1:20, and the preparation time was 1 hour. The yield of vitamin E acetate was 86.85%. The synthesis process of VEA was optimized by single factor experiments such as substrate molar ratio, reaction time, dosage of nano enzyme, reaction temperature and so on. The results showed that the yield of vitamin E acetate reached 96.31% under the optimal conditions of n (D-α-tocopherol) =1mmol, [n (D-α-tocopherol) : n (Acetic anhydride)] =1:15, reaction time 24 h, ZIF-8 dosage 0.05 g, reaction temperature 50 °C.
研究领域:
化学
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生物学
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产品关联
DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 13
·2483
·
IF 5.2
Qian Zou,Weifei Wang,Qingqing Xu,Menglei Yan,Dongming Lan,Yonghua Wang
α-Tocopherol has been widely used in medicine, cosmetics, and food industry as a nutritional supplement and antioxidant. However,α-tocopherol showed low bioaccessibility, and there is a widespreadα-tocopherol deficiency in society today. The preparation of oil-in-water emulsions with high safety and low-calorie property is necessary. The aim of this research was to investigate the effects of different protein emulsifiers (whey protein isolate (WPI), soy protein isolate (SPI), and sodium casein (SC)) on the properties of emulsions delivery system, and diacylglycerol (DAG) was picked as a low-accumulated lipid. The interfacial changes, microstructural alterations, and possible interactions of the protein-stabilized DAG emulsions were investigated during the in vitro digestion. The results show that different proteins affect the degree of digestibility andα-tocopherol bioaccessibility of the emulsions. Both WPI- and SPI-coated emulsions showed good digestibility andα-tocopherol bioaccessibility (77.64 ± 2.93%). This might be due to the strong hydrolysis resistance of WPI (β-lactoglobulin) and the good emulsification ability of SPI. The SC-coated emulsion showed the lowest digestibility andα-tocopherol bioaccessibility, this might be due to the emulsification property of hydrolysis products of SC and the potential interaction with calcium ions. This study provides new possibilities for the application of DAG emulsions in delivery systems.Keywords:diacylglycerol;in vitro digestion;bioaccessibility;α-tocopherol;delivery systems
研究领域:
食品科学
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营养学
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药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2020
·Vol. 100
·期 11
·4319-4328
·
IF 2.614
Tengfei Zhan,Yunsheng Han,Chaohua Tang,Qingyu Zhao,Dandan Sun,Ying Li,Xueting Jia,Lingyun Zhou,Junmin Zhang
BACKGROUND The aim of this study was to investigate the metabolism of α -tocopherol derived from vitamin E-enriched transgenic maize (VER) and its effects on antioxidant and immune functions in broilers aged 1–42 days. A total of 360 1-day-old male broilers were randomly divided into three groups containing six replicates with 20 broilers per replicate. The negative control (NC) group and the positive control (PC) group were given non-GM maize and non-GM maize plus exogenous vitamin E (VE), respectively, and the VER group was given VER, replacing the non-GM maize given to the NC group. Between days 1 and 21 and days 22 and 42, VE levels were 4.38 and 4.63 mg kg −1 in the NC group, and 14.11 and 14.91 mg kg −1 in the PC and VER group, respectively. RESULTS The results showed that α -tocopherol from both VER and additives increased α -tocopherol transfer protein and cytochrome P450 concentrations. Serum α -tocopherol and α -tocopherylquinone levels of broilers in the PC and VER groups were also significantly higher than those in the NC group ( P < 0.05). Compared with the NC group, broilers in both groups that received α -tocopherol had reduced NF- κ B p65 concentrations, significantly decreased serum prostaglandin E 2 , interleukin-6, malondialdehyde, and hydrogen peroxide levels ( P < 0.05), and significantly increased glutathione, glutathione peroxidase, and total antioxidant capacity ( P < 0.05). CONCLUSION In summary, both VER and non-GM maize fortified with exogenous VE showed similar effects on broilers, indicating that the α -tocopherol in VER has sufficient biological activity. © 2020 Society of Chemical Industry
研究领域:
动物营养
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基因工程
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2015
·Vol. 18
·期 5
·1240-1248
·
IF 8.02
Yifeng Tao,Ruijuan Dong,Ioannis V. Pavlidis,Biqiang Chen,Tianwei Tan
Vitamin E (VE) has significant biological activities and thus its acylation to increase its stability is of extreme interest. We developed an efficient and sustainable approach using imidazolium-based ionic liquids as dual solvent-catalysts for the esterification between α-tocopherol (the most active form of VE) and succinic anhydride. Although in literature it is reported that lipase can catalyze this reaction, hereby we demonstrate that the reaction observed in DMSO and DMF is catalyzed by the histidyl residues of the protein. Histidine and its analogue containing an imidazole ring were tested as organocatalysts for the production of α-tocopherol succinate. In light of the imidazole organocatalysis, commercially-available 3-alkyl-1-methyl imidazolium ILs [CnC1Im][X−] were investigated as dual solvent-catalysts for the esterification of α-tocopherol with succinic anhydride, and provided satisfactory yields and reaction rates. [C5C1Im][NO3−] can be recycled by water extraction, instead of organic solvent extraction to separate α-tocopherol succinate from [C5C1Im][NO3−], with an average yield of 94.1% for 4 subsequent batches, while the catalytic activity of the recycled ILs showed almost no loss after 4 batches. The developed protocol for the synthesis of α-tocopherol esters and IL recycling bears industrial potential due to the ease of use and the efficient recycling.
研究领域:
有机化学
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生物化学
·
绿色化学
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产品关联
DOI
高影响因子
JOURNAL OF POROUS MATERIALS
·
2020
·Vol. 27
·期 6
·1639-1648
·
IF 2.183
Zhang Tiantian,Li Bin,Zhang Xiaoli,Wang Jiao,Wei Longhui,Zhao Binxia,Li Binglin
N,N′-dimethyl-N-(4-pyridyl)-1,2-ethanediamine (DMPE) as a special N-alkylation precursor of 4-dimethylaminopyridine (DMAP) was grafted on the surface of γ-chloropropylated functionalized mesoporous silica MCM-41 to prepare immobilized DMAP. Characterization results of small angle X-ray diffraction, thermogravimetry and derivative thermogravimetry, Fourier transform infrared, scanning electron microscopy, energy-dispersive X-ray and transmission electron microscopy indicated that DMAP was successfully covalently bound to the surface of MCM-41. The highest loading of DMAP reached 0.9 mmol/g, which was 2.5 times higher than that of DMAP immobilized on the normal silica gel. The prepared immobilized DMAP as nucleophilic heterogeneous catalysts was successfully used for the acetylation reaction to produce α-tocopherol acetate. The highest yield reached 90.1% under the optimal condition which was summarized as follows: immobilized DMAP 100 mg, substrates mole ratio (acetic anhydride/α-tocopherol) 3:1, reaction temperature 50 ℃, stirring intensity 500 rpm, reaction in toluene for 12 h. The recyclability and stability of the DMAP grafted MCM-41 were excellent, as demonstrated by its use 20 times during batch operation without any loss of productivity.
研究领域:
化学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）