Agronomy-Basel
·
2026
·Vol. 16
·期 4
·
IF 4.1
Haibin Chen,Lixin Lin,Huang Li,Bangyu Huang,Peng Cui,Ruqin Fan,Jianjun Du
Oxalic acid is a key root exudate released by plants under phosphorus (P) deficiency and plays a direct role in solubilizing fixed soil P. However, its specific effects on soil microbial community assembly and ecological functions remain less clear. In this study, based on anex plantasoil microcosm incubation experiment, the impacts of oxalic acid input on soil bacterial and fungal community assemblage and functional profiles involved in P mobilization were explored. The results showed that oxalic acid input significantly changed soil bacterial and fungal community composition, decreased their diversity, and enriched bacterial taxa involved in P mobilization and fungal taxa associated with plants, showing the selective effects of oxalic acid on soil microorganisms. Further community assembly analyses (βNTI and NST) showed that oxalic acid input promoted a shift in bacterial community from a stochastic-process-dominated community to a deterministic-process-dominated community, while the fungal community exhibited a converse pattern. These findings reveal the important role of oxalic acid in shaping soil microbial community assembly and ecological functions under P deficiency, broadening our understanding of the role of oxalic acid in plant responses to low-P stress.
✓ 已验证
产品关联
DOI
高影响因子
Advanced Science
·
2026
·
IF 14.1
Xiang Gao,Zhijun Yu,Yu Yan,Jiamin Ding,Wangsiyuan Teng,Chenhe Zhou,Minjun Yao,Wenkan Zhang,Shenzhi Zhao,Fangqian Wang,Zhenxuan Shao,Jinfeng Zhou,Xiaoyong Wu,Chengcheng Yu,Liang Chen,Xiaoqiang Jin
3D cell infiltration into porous scaffolds constitutes a fundamental prerequisite for bone tissue engineering. Though pore size and curvature are known to dictate this process, their mathematical coupling remains elusive. Herein, we identified a size-dependent bone marrow-derived mesenchymal stem cells 3D in-growth pattern in which small pores promoted horizontal bridging, while large pores favored vertical cellular migration into the scaffold core. An analytical framework of Porous-Fisher model was developed using a superposition approach tailored to boundary-specific solutions. This approach not only enabled quantitative prediction of coverage rates through examination of grid dimensions and diffusion coefficients but also mathematically elucidated curvature and strategic geometric design. Furthermore, the prediction of cellular diffusion patterns on porous scaffolds was achieved through the alteration of boundary conditions and diffusion coefficients. Convex topological configurations were shown to accelerate cellular infiltration, whereas concave geometries permitted spatiotemporal modulation of tissue growth. Additionally, lower diffusion environments delayed coverage, suggesting scaffold designs with reduced pore sizes might benefit elderly patients. Consequently, the accuracy of model was in vivo validated by a rat cranial defect model. Overall, the mathematical model provided an effective way for ideal pore structure prediction in advance and propel the application of porous scaffolds in tissue engineering.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2026
·
IF 4.7
Lei Kang,Chunquan Ma,Xiajie Hu,Xudong Wu,Rong Shan,Fuxiang Song
Bone tissue engineering scaffolds are used to repair dimensional alveolar crest defects resulting from etiologies such as trauma, infection, or tumor resection, thereby enabling a more accurate, efficient, and predictable dental diagnosis and treatment. In this study, cone beam computed tomography (CBCT) data from patients with alveolar crest defects were utilized to design a personalized scaffold. Subsequently, the 3D PCL/β-TCP scaffold was successfully fabricated based on this design using 3D printing technology. In parallel, a comprehensive series of assays were conducted to evaluate the properties of the 3D PCL/β-TCP scaffold, including its ability to promote MC3T3-E1 cell adhesion and proliferation in vitro. Furthermore, its capacity to induce bone formation was assessed in an in vivo animal model. The results demonstrated that the scaffold exhibited excellent biocompatibility, as evidenced by CCK-8 and live/dead (AM/PI) fluorescence staining assays, and significantly facilitated the adhesion and proliferation of MC3T3-E1 cells. Additionally, ALP and Alizarin Red staining experiments indicated that the 3D PCL/β-TCP scaffold significantly enhanced ALP expression and calcified nodule formation. Finally, the in vivo experiments in rats with skull defects confirmed that the 3D PCL/β-TCP scaffold contributed to accelerated bone formation. In summary, owing to numerous favorable properties such as personalized customization, biomimetic porous architecture, and excellent osteogenic ability, the 3D PCL/β-TCP scaffold was confirmed to be an advantageous candidate, offering ideas and effective methods for the precise repair of the alveolar crest.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Zijian Yan,Yongan Lin,Jiayi Chen,Ruibin Lin,Zhuhao Tan,Yunqi Fu,Feifan Luan,Chenxiao Zheng,Li Ren
Thrombin-triggered in situ anchoring enables stable underwater scaffold fixation for 48 h. • Bilayer biomimetic scaffold with strong water absorption captures and retains BMSCs. • CPTT@PRP synergistically upregulates CCL2 and CXCL8 to recruit BMSCs. • CPTT@PRP restores hyaline cartilage anisotropy. Utilising endogenous stem cells with bioactive biomimetic scaffolds to achieve in situ osteochondral regeneration represents a promising strategy in contemporary regenerative medicine. However, existing scaffold fixation methods (e.g., sutures, screws, or photocrosslinking) often cause secondary damage, involve complex procedures, or exhibit poor biocompatibility, and struggle to achieve efficient capture and long-term recruitment of stem cells from bone marrow blood. Herein, we propose a novel concept of “thrombin-triggered in situ anchoring” and integrate it into a type I collagen-based biomimetic bilayer osteochondral scaffold. Upon implantation, the thrombin loaded within the scaffold rapidly catalyzes the conversion of fibrinogen (released after microfracture) into a fibrin network, thereby achieving immediate and stable in situ fixation at the defect site. This adhesive property can be maintained underwater for up to 48 h, avoiding the drawbacks of conventional fixation methods. Concurrently, the scaffold exhibits a “sponge-like” strong water-absorption effect. After implantation into the microfractured defect, it rapidly absorbs bone marrow blood, maximizing the capture and retention of bone marrow mesenchymal stem cells (BMSCs). The sustained release of PRP further establishes a chemotactic gradient that actively guides the directional migration of BMSCs and modulates the microenvironment to induce their bidirectional differentiation. RNA sequencing results indicate that the scaffold synergistically upregulates CCL2 and CXCL8, promoting BMSC chemotaxis. In a rabbit critical-sized osteochondral defect model, CPTT@PRP achieved hyaline-like cartilage regeneration and subchondral bone repair within 12 weeks, improved collagen organization. This work applies the concept of enzymatic in situ anchoring to the design of osteochondral regeneration scaffolds, providing a novel strategy for microfracture-based osteochondral repair, and holds promise for extension to other fields of tissue engineering.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 16
·3713-3726
·
IF 7
Qiao Bian,Chao Guo,Shuquan Cui,Jia Liu,Guohua Xu,Wei Feng
Bone tissue engineering, as an important and attractive multidisciplinary field, affords a feasible strategy for large bone defects which are difficult to heal without clinical intervention. However, the complicated requirements of bone regeneration result in the imperfect performance of many current materials. Inspired by the composite nature of bone tissues, we proposed an organic–inorganic composite strategy. Specifically, we loaded a bone regeneration drug (simvastatin, SIM) and an inorganic component (strontium hydrogen phosphate (SrHPO4)/beta-tricalcium phosphate (β-TCP)) to a thermogel, constituted by poly(ε-caprolactone-co-D,L-lactide)–poly(ethylene glycol)–poly(ε-caprolactone-co-D,L-lactide) (PCLA–PEG–PCLA), with a thermo-induced sol–gel transition, to prepare an injectable composite for bone regeneration in cranial defects. The SIM/(Sr/β-TCP)/PCLA–PEG–PCLA composite was able to be conveniently injected and it spontaneously filled the defect. The appropriate mechanical strength obtained by thermogelation under the stimuli of the body temperature provided the necessary support while the capacity of loading drugs and bioceramics offered bioactivity and osteoinduction. In vitro drug release experiments demonstrated a gentle and sustained release of SIM for as long as 2 months, benefitting bone regeneration. The excellent capacity of promoting the formation of osteocytes was proved by cell differentiation assays. Furthermore, the enhanced bone regeneration capacity was verified by the micro-CT results of rat cranial defects with implantation time. Based on our results, the organic–inorganic composite integrates the advantages of each component and is far beyond, thus it might serve as a promising material candidate for bone regeneration.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2022
·
IF 7.968
Lizheng Jin,Min Chen,Yufeng Wang,Yaqi Peng,Qi Yao,Jiamin Ding,Binbin Ma,Shengyong Lu
With the rapid development of municipal solid waste (MSW) incineration, the effective disposal and recycling utilization of fly ash is attracting increasing attention. The mechanochemical (MC) treatment as a non-thermal method shows good stabilization of heavy metals and degradation of dioxins in fly ash. The inhibition effect and long-term safety of CaSO 4 , Ca 3 (PO 4 ) 2 , NaH 2 PO 4 , and composite additives (50 %NaH 2 PO 4 +50 %CaO) as additives for heavy metals stabilization were investigated. The composite additive showed the best inhibitory effect on heavy metals, and the leaching concentration of Cd, Cr, Cu, Ni, Pb, and Zn were decreased by 95.21 %, 46.84 %, 99.42 %, 49.91 %, 99.83 %, and 99.55 %, respectively. In addition, the modification by MC treatment reduced the particle size and increased the specific surface area of fly ash and improved the activity for resource utilization. Therefore, MC treated fly ash is used as supplementary cementitious material (SCM), which will produce a filling effect and pozzolanic effect improving the performance of fly ash concrete blocks. The compressive strength of the MC-treated fly ash concrete blocks curing for 28 days increased by 35.5 % and exceeded 40 MPa, close to OPC’s strength. The concrete block also had a secondary inhibition on potentially toxic elements, making the resource utilization of fly ash more stable and safer. In general, the MC treatment and the utilization of MC-treated fly ash as SCM effectively inhibit the leaching of heavy metals in fly ash, delivering a promising method for green fly ash disposal.
✓ 已验证
产品关联
DOI
高影响因子
Pharmaceutics
·
2022
·Vol. 15
·期 1
·88
·
IF 6.525
Xialin Li,Xingyu Huang,Long Li,Jiayi Wu,Weihong Yi,Yuxiao Lai,Lei Qin
Increased multiantibiotic-resistant bacteria means that infected bone defects remain a significant challenge to clinics. Great interest has emerged in the use of non-antibiotic antimicrobials to reduce the rate of multiantibiotic-resistant bacterial infection and facilitate bone regeneration. The cationic antimicrobial peptide LL-37 is the sole human cathelicidin and has shown nonspecific activity against a broad spectrum of microorganisms. In this study, we fabricated the poly(lactic-co-glycolic acid)/β-calcium phosphate/peptide LL-37 (PLGA/TCP/LL-37, PTL) scaffold with low-temperature 3D-printing technology for the treatment of infected segmental bone defects. The prepared scaffolds were divided into three groups: a high LL-37 concentration group (PTHL), low LL-37 concentration group (PTLL) and blank control group (PT). The cytocompatibility and antimicrobial activity of the engineered scaffolds were tested in vitro, and their osteogenesis properties were assessed in vivo in a rat infected bone defect model. We found the fabricated PTL scaffold had a well-designed porous structure that could support a steady and prolonged LL-37 release. Furthermore, the PTHL group showed strong antibacterial activity againstStaphylococcus aureus(S. aureus) andEscherichia coli(E. coli) without any inhibition of the proliferation or alkaline phosphatase activity of rat bone marrow mesenchymal stem cells (BMSCs) in vitro. In addition, the infected femoral defects implanted with PTHL group displayed new bone formation in four weeks without any evidence of residual bacteria, which showed similar antibacterial outcomes to the vancomycin and cancellous bone mixture group. In conclusion, the PTHL composite scaffold is a promising non-antibiotic antimicrobial graft with good biodegradability, biocompatibility, and osteogenic capability for infected bone defects.Keywords:peptide LL-37;non-antibiotic antimicrobial graft;PLGA/TCP/LL-37 scaffolds;low-temperature 3D-printing technology;rat infected femoral defect model
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2023
·
IF 8.4
Hao Wang,Xinyu Xu,Xiaohan Mei,Dapeng Zeng,Boda Ying,Zehao Yu,Shibo Liu,Ruiyan Li,Yanguo Qin
Bone implants that promote osseointegration and have antimicrobial capabilities are necessary to ensure optimal long-term functionality of implants. In this study, a porous composite osteogenic scaffold was prepared using a twin-screw extruder and a fused deposition modeling three-dimensional (3D) printer. A polydopamine (PDA) coating was used to load graphdiyne oxide (GDYO) on the surface of the 3D printed polyetherimide-tricalcium phosphate (PEI-TCP) osteogenic scaffold through π–π double-bond interactions. The resulting P-T-GDYO composite scaffold was characterized, and its mechanical properties, ability to promote osteogenic differentiation, and light-responsive antibacterial activity were evaluated. When the scaffold was doped with 10 wt% β-TCP, its mechanical properties remained in the range of those of cancellous bone. In terms of osteogenic differentiation, the P-T-GDYO composite scaffold promoted adhesion and osteogenic differentiation of MC3T3-E1 cells, as well as effectively promoting endogenous bone regeneration of rabbit femoral condyle bone defects in osteogenesis experiments in vivo . In a mouse subcutaneous infection model, after near-infrared light irradiation, P-T-GDYO showed an antibacterial rate of 90.18 % against Staphylococcus aureus , while maintaining good tissue–organ compatibility. This study represents an important development in GDYO-based photothermal antibacterial materials in the field of orthopedics and expands its application in the biomedical field.
✓ 已验证
产品关联
DOI
高影响因子
GREEN CHEMISTRY
·
2023
·Vol. 25
·期 16
·6297-6305
·
IF 9.8
Zhaohui Guo,Christian Marcus Pedersen,Honghong Chang,Yingxiong Wang,Yan Qiao
As a renewable source, fructose is of great significance in biomass conversion and food industries. Currently, most of the chemo-catalysts studied, with glucose as a substrate, are limited by the thermodynamic equilibrium of the reaction, resulting in the maximum yield of fructose of approximately 30–40%. The similar chemical structures and physical properties of monosaccharides bring another challenge for the separation of fructose from the reaction mixture. In this study, the combination of CaCl2 and Et3N was found to alter the thermodynamic equilibrium and promote fructose separation. The reaction parameters were optimized and up to 78% fructose yield could be obtained. The pathways of the isomerization reaction were found to involve both intramolecular hydride shift and an enediol intermediate mechanism, and the former was major. It could be speculated reasonably that Ca2+ played a key role in glucose conversion and interacted with glucose at its C1 and C2 positions, and Et3N interacted with Cl− while providing an alkaline environment. The formation of the α-fructofuranose’ complex, from Et3N, CaCl2 and α-fructofuranose, could shift the equilibrium of glucose isomerization towards fructose formation. According to the diffusion-ordered spectroscopy (DOSY) NMR results, the strong complexation slowed down the diffusion rate of the α-fructofuranose’ complex, allowing it to gradually precipitate, and hence drove the reaction equilibrium towards the desired fructose. The precipitate was filtered from the reaction mixture and processed further to obtain high-purity fructose in 45% isolated yield. Moreover, the filtrate containing solvent and reagents obtained in the separation process could be recycled.
✓ 已验证
产品关联
DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2023
·
IF 5.2
Xijun Li,Luna Ruan,Lihua Zhu,Jun Liu,Huan Zhang
Herein, we synthesized PtCo/CNTs, Pt/CNTs, and Co/CNTs nanocatalysts at room temperature by chemical reduction method. The synergistic effect in PtCo/CNTs for crotonaldehyde (CAL) hydrogenation is investigated. The nanostructures , morphologies, electronic property, Pt–Co interaction, surface characteristic and composition of the catalysts are examined by XRD , BET, SEM, XPS, HRTEM , STEM, STEM-EDX elemental maps and line profiles and H 2 -TPD. It is found that Pt and Co forms PtCo bimetallic nanoparticles in the PtCo/CNTs catalyst and the PtCo bimetallic nanoparticles are coated on multi-walled carbon nanotubes. And PtCo/CNTs achieves 83.5% CAL conversion, 91.3% crotyl alcohol (COL) selectivity, and 647.4 h −1 turnover frequency (TOF) at 60 °C within 2 h under 3 MPa hydrogen, and its catalytic performance remains basically unchanged after five recycles. According to the analysis, the presence of PtCo bimetallic particles lead to electron transferring from Co to Pt (synergy effect), which improves their activation ability of hydrogen and desorption of C C in COL and thus enhances its selectivity to COL.
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Jie Yang,Yandi Tang,Huimei Pan,Wei Ma,Wang Luo,Baiyang Chen,Yinan Bu
Total organic halogen (TOX) analysis is essential to monitor the formation of all halogenated organics in water. Currently, TOX needs to be separated by tailored activated carbon and then converted by combustion before determination, which have some inherent drawbacks and multiple complicated phase transition processes that prevent its widespread application. To overcome these issues, this study proposed a facile alternative TOX analytical method based on a fundamental water chemistry principle. That is, silver carbonate was used to remove halides from water firstly by forming silver halides precipitates, and then TOX remaining in water was dehalogenated by vacuum ultraviolet photolysis before determination of TOX by ion chromatography. Since the keys to success for the proposed approach are to maximize the removal of halides and to minimize the loss of TOX in water, a series of operation variables were evaluated and optimized. Under optimal conditions, the residual Cl – and Br – levels in treated water were less than 57.5 μg-Cl/L and 42.7 μg-Br/L, respectively. Meanwhile, the method achieved satisfactory recoveries of model organic halogen compounds (30.0–1000.0 μg/L) in both synthetic water (92.0–101.0 %) and tap water (98.0–103.6 %). Because this method uses only inexpensive reagents and handy operations without any phrase-changing for target analyte, it may serve as a routine means for detecting TOX in the future.
✓ 已验证
产品关联
DOI
高影响因子
CONSTRUCTION AND BUILDING MATERIALS
·
2022
·
IF 7.693
Hongwei Li,Rui Wang,Wei Zhao,Hongwei Guo,Bingji Yan,Peng Li
Lightweight glass-ceramic foams as novel building and decoration materials exhibit great advantages. Herein, fluorite tailings and waste glass were recycled to sinter glass-ceramic foams by introducing calcined kaolin and calcium phosphate. The effects of calcium phosphate addition and sintering temperature on the crystalline phase, microstructure evolution, fluoride leaching performance and physical properties of the glass-ceramic foams were systematically investigated using X-ray diffraction, scanning electron microscopy, energy dispersive spectroscopy, Fourier transform infrared spectra, computed tomography, and differential scanning calorimetry. The obtained glass-ceramic foams sintered at 1110 °C exhibited relatively homogeneous pore sizes and distributions, low densities and thermal conductivities, and high compressive strengths. Moreover, the addition of calcium phosphate promoted the formation of fluorapatite, which benefited the toxic fluorine stabilization in the glass-ceramic foams. Thermodynamic data were calculated using Factsage 8.1 and verified through experiments to establish the physical structure transition during sintering. This study provides an efficient utilization route to convert fluorite tailings and waste glass into stable and valuable lightweight glass-ceramic foams.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）