ACTA MATERIALIA
·
2025
·
IF 9.3
Yanjun Liu,Yu Pan,Ranpeng Lu,Aihua Yu,Shuya Zhao,Jianzhuo Sun,Xin Lu
Improving the room-temperature ductility of TiAl alloys is crucial for their broader structural applications. However, excessive interstitial oxygen (O) contamination intensifies the strain incompatibility between α 2 and γ phases, severely impairing the overall mechanical properties. Here, we propose a novel calcium (Ca)-based O-scavenging strategy to enhance the ductility of powder metallurgy (PM) TiAl alloys by introducing trace CaH 2 . A duplex oxide layer composed of outer Al 2 O 3 layer and inner TiO 2 layer, was identified on the surface of TiAl powder and has the thickness of ∼9.13 ± 0.35 nm. Upon the dissolution of oxide layer (∼824°C), O is released and readily diffuses into the matrix as interstitials, where it becomes effectively irretrievable. CaH 2 can react with the oxide layer below its decomposition temperature (∼791°C), effectively immobilizing O as nanoscale CaO particles, thereby suppressing O dissolution into α 2 phase and mitigating the lattice distortion. The resulting purification of the α 2 phase decreases the critical resolved shear stress (CRSS) required for basal slip. During deformation, deformation twins in the adjacent γ grains facilitate the activation of -type dislocations within α 2 phase, thereby enhancing its plasticity and improving interphase strain compatibility. Additionally, the in-situ formed CaO particles contribute to the dispersion strengthening. As a result, this trace Ca-based deoxidation strategy nearly doubles the room-temperature total elongation of PM Ti4822 alloys while retaining an ultimate tensile strength of 580 MPa, even at a relatively high O content (∼1520 ppm).
研究领域:
材料科学
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金属学与冶金工程
·
结构材料
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粉末冶金
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力学性能
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位错理论
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相变与组织调控
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高影响因子
Applied Physiology Nutrition and Metabolism
·
2018
·Vol. 44
·期 1
·
IF 2.518
Yu Qiao,Guoqiang Fan,Jun Guo,Shixing Gao,Ruqian Zhao,Xiaojing Yang
Zinc-α2-glycoprotein (ZAG) has been demonstrated to play a role in stimulating lipid mobilization under normal conditions. However, further studies are required to determine whether ZAG overexpression can alleviate the reduction in plasma lipid levels under stress conditions. In the present study, we investigated the effects of ZAG on lipometabolism in white adipose tissue (WAT) after dexamethasone (DEX) stimulation using C57BL/6 male mice as the experimental models. Transcript and protein levels of genes associated with the β-adrenoreceptor (β-AR)/cyclic adenosine monophosphate/protein kinase a (PKA) pathway, lipid mobilization, and energy metabolism were determined by quantitative real-time polymerase chain reaction and Western blotting. Plasma levels of nonesterified fatty acid (NEFA) were measured using an automatic biochemical analyzer. Results indicated that plasma NEFA levels were decreased in the DEX group, but NEFA levels were rescued by ZAG overexpression. ZAG overexpression resulted in the upregulation of β3-AR and phosphorylated PKA protein relative to those of the DEX group. Analysis of lipometabolism showed that protein levels of phosphorylated hormone-sensitive lipase was reduced upon DEX treatment but were restored by ZAG overexpression. For energy metabolism, ZAG significantly upregulated the protein expression of carnitine palmitoyltransferase1a and cytochrome c oxidase subunit 1 relative to those of the DEX group. In conclusion, ZAG could alleviate DEX-induced decrease in plasma NEFA levels and this could be associated with the promoting lipid mobilization in WAT.
研究领域:
药理学
·
内分泌学
·
代谢
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DOI
PubMed
高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2024
·
IF 5.3
Aijun Qian,Gengmiao Xiao,Zhuang Li,Yunping Mu,Xiaohong Liu,Xue Tian,Jianqin Yang,Allan Z. Zhao,Fanghong Li
The escalating obesity epidemic poses serious public health challenges, requiring the development of effective therapeutic strategies. In this study, we aimed to determine if recombinant glycoprotein hormone β5 (GPHB5) protein, particularly in the hybrid form with glycoprotein hormone α2 (GPHA2) (recombinant corticotroph-derived glycoprotein hormone, rCGH), can alleviate obesity in the genetically obese mice, ob/ob . Six-week-old male ob/ob mice were intraperitoneally injected for four weeks with rCGH (10 mg/kg) treatment. Body weight, fat mass and lean mass as well as energy expenditure were evaluated. Blood samples were collected to assess circulating concentrations of triiodothyronine (T3) and thyroxine (T4). Glucose and insulin tolerance tests were also conducted. rCGH significantly decreased body weight and fat mass, stimulated energy expenditure without alterations in food consumption, induced lipolysis and browning of white adipose tissue (WAT) by activating cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) signaling pathway. The treatment with the recombinant protein also led to marked reduction in hepatic steatosis, improved glucose tolerance and insulin sensitivity, and reduced triglycerides (TG), and total cholesterol (T-CHO) levels in ob/ob mice. In conclusion, rCGH attenuated obesity and alleviated metabolic syndromes in ob/ob mice, and it may represent a promising polypeptide-based drug candidate in treating obesity and obesity-related complications without interfering energy intake.
研究领域:
内分泌学
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代谢研究
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肥胖研究
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药理学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Qihao Zhu,Weiran Mao,Chengli Zhang,Yuan Zhou,Zhiyong Tang,Chao Yu
Here, a novel electrochemiluminescence biosensor based on potential-resolved strategy was firstly prepared for the detection of dual targets α2,3-sialylated glycans and α2,6-sialylated glycans. This is the first time that Au@BSA microsphere was used to connect with luminol to enhance its ECL intensity, and it can generate ECL signals at positive potential. Zeolitic Imidazolate Framework-8 (ZIF-8) and Meso-tetra (4-carboxyphenyl) porphyrin (TCPP) were linked using a one-pot method to synthesize a novel luminescent ZIF (L-ZIF) named TZZ, which can emit ECL signals at negative potential. Moreover, magnetite microspheres were used to construct a sandwich-type biosensor to obtain higher sensitivity and reduce background signals. In addition, the biosensor manufactured directly in solution have a wider linear range than constructed on electrode because it has more available space than the electrode surface. Due to the above advantages, the prepared ECL biosensor exhibited high sensitivity, stability and broader linear range, even for practical analysis. Therefore, the prepared ECL biosensor will become a promising method for determination of α2,3-sialylated glycans and α2,6-sialylated glycans in clinical applications in the future. What is more, it provides a potential method for detection of other multi-targets.
研究领域:
分析化学
·
生物医学工程
·
传感技术
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DOI
PubMed
高影响因子
METABOLIC BRAIN DISEASE
·
2018
·Vol. 33
·期 5
·1721-1731
·
IF 2.441
Chen Zhao-Di,Chen Wen-Qiang,Wang Zhi-Yuan,Cao Dan-Ni,Wu Ning,Li Jin
Previous studies have shown that agmatine, a potential neuromodulator or co-transmitter, exhibited antidepressant-like action in animal models, yet its mechanism, especially the receptor mechanism, remains unclear. In the present study, using efaroxan, a preferential antagonist of I 1 imidazoline receptor (I 1 R) and yohimbine, an antagonist of α 2 adrenergic receptor (α 2 AR), we investigated the roles of I 1 R and α 2 AR in agmatine’s antidepressant-like effect in acute and sub-acute depression models in mice. We found that in the tail-suspension test (TST) and the forced swimming test (FST), acute administration of agmatine (20 and 40 mg/kg, p.o.) significantly shortened the immobility time. Concurrent administration of efaroxan (1 mg/kg, i.p.) completely abolished the antidepressant-like effects of agmatine (40 mg/kg, p.o.) whereas yohimbine (5 mg/kg, i.p.) failed to exert similar effects, suggesting that the acute antidepressant-like effects of agmatine was mainly mediated by I 1 R but not α 2 AR. Additionally, in the learned helplessness (LH) test, repeated administration of agmatine (20 mg/kg, p.o., q.d.) for 5 days significantly decreased the escape latency and the number of escape failure, and these effects were respectively abolished by concurrent administration of efaroxan (0.5 mg/kg,i.p., q.d.) and yohimbine (3 mg/kg, i.p., q.d.) for 5 days, suggesting that the antidepressant-like action of agmatine in the LH test was achieved via the activation of both I 1 R and α 2 AR. In summary, we found that the antidepressant-like effects of agmatine in the TST and the FST were mediated by activating I 1 R and in the sub-acute LH test were mediated by activating both I 1 R and α 2 AR.
研究领域:
神经科学
·
药理学
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DOI
PubMed
高影响因子
CNS Neuroscience & Therapeutics
·
2025
·Vol. 31
·期 1
·e70197
·
IF 5
Weiwei Rong,Xunjia Qian,Yujian Yin,Yipeng Gu,Weiyi Su,Jie‐Jia Li,Yue Xu,Hongyan Zhu,Junxu Li,Qing Zhu
NDSM demonstrates good analgesic efficacy in preclinical pain models with fewer side effects than sinomenine. The analgesic effects of NDSM are mediated primarily by GABRA2 and partially by GABRA3. NDSM shows promise as a novel analgesic candidate for chronic pain management.
研究领域:
药理学
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疼痛研究
·
神经科学
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DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 50
·44865-44872
·
IF 3.289
Yazhen Niu,Junlin He,Yuliang Li,Yilin Zhao,Chunyong Xia,Guolin Yuan,Lei Zhang,Yuchan Zhang,Chao Yu
Sialylated glycans are crucial molecular targets for cancer diagnosis and clinical research. α2,3-Sialylated glycans and α2,6-sialylated glycans are the predominant sialic acids found in nature. Different expression of the quantity of glycans can result in development of different disease. However, there are no ideal methods for discriminating α2,3-sialylated glycans and α2,6-sialylated glycans. In this work, a multi-purpose biosensor is fabricated for sensitive detection of α2,3-sialylated glycans and α2,6-sialylated glycans. To improve the sensitivity of the biosensor, p-MWCNTs were integrated with PAMAM, as PAMAM has highly branched and abundant amino groups, providing a large available surface area for linking with other substances. To achieve distinguishable recognition, Maackia amurensis lectin (MAL) and Sambucus nigra agglutinin (SNA) were included. To facilitate the lectin fixation, PDITC, a kind of green homobifunctional cross-linker, was selected. Under optimized detection conditions, the linear range of detection for α2,3-sialylated glycans is 10 fg mL−1 to 50 ng mL−1 with a lower detection limit of 3 fg mL−1, and the linear range of detection for α2,6-sialylated glycans is 10 fg mL−1 to 50 ng mL−1 with a detection limit of 3 fg mL−1. This work not only provides a method for distinguishing detection of α2,3-sialylated glycans and α2,6-sialylated glycans, but also provides a reference for future clinical testing.
研究领域:
生物化学
·
分析化学
·
生物医学工程
·
临床诊断
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DOI
高影响因子
ARCHIVES OF PHARMACAL RESEARCH
·
2026
·
IF 8.2
Fang Haihong,Wang Shuang,Ma Zhuo,Pei Youming,Chen Ying
This study investigated whether dexmedetomidine (Dex) attenuates sepsis-induced inflammation and organ injury in association with hepatic 4-hydroxyphenylpyruvate dioxygenase (HPD) regulation through α2-adrenergic receptor (α2-AR) signaling. Using an integrative approach combining in vivo and in vitro models, single-cell RNA sequencing, and 16S rRNA analysis, we found that Dex suppressed NET formation, reduced pro-inflammatory cytokines, and ameliorated liver, kidney, and lung injury in a murine cecal ligation and puncture (CLP) model. Dex decreased hepatic HPD expression, which was accompanied by reduced hepatic HIF-1α levels. Genetic experiments showed that HPD silencing phenocopied several protective effects of Dex, while HPD overexpression weakened Dex’s ability to inhibit NETs and provide organ protection. Conditioned-medium experiments indicated that hepatocyte-derived soluble factors convey HPD-associated signals to neutrophils; the inhibitory activity of sh-HPD conditioned medium was heat-sensitive, enriched in the > 3 kDa fraction, and linked to reduced p47phox membrane translocation. Supplementation with 4-hydroxyphenylpyruvate partially restored neutrophil ROS generation, suggesting a role for HPD-related metabolic imbalance. Multi-omics analyses showed that Dex treatment reduced intestinal Th17 cell differentiation, increased Treg proportions, and remodeled gut microbiota composition. These microbiome changes are interpreted as supporting gut-immune remodeling rather than an independent causal factor. In summary, our findings support a hepatocyte-centered immunometabolic model in which Dex treatment is associated with α2-AR-dependent HPD regulation, altered hepatic HIF-1α levels, reduced neutrophil oxidative burst and NET formation, and attenuation of Th17 polarization and organ injury in sepsis.
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高影响因子
Molecular Omics
·
2021
·Vol. 17
·期 4
·565-571
·
IF 3.743
Lujie Yang,Quanqing Zhang,Ling Lin,Ying Xu,Yuanyu Huang,Zuojian Hu,Ke Wang,Cuiping Zhang,Pengyuan Yang,Hongxiu Yu
Glycosylation is a widely occurring posttranslational modification. Here, we applied a quick, convenient and high-throughput strategy (lectin array) to investigate the variation in glycans on different macrophage subtypes derived from THP-1 and RAW264.7 cells. For THP-1 cells, there were more significant differences in the glycan on M2 macrophages compared to the other two subtypes. In contrast, M1 macrophages exhibited more significant glycan remodeling than the other subtypes for the RAW264.7 cell line. The response of the lectins which recogonize the N-glycan and α2,6 sialic acid was higher during polarization into anti-inflammatory phase (THP-1 derived M2 subtypes), and lower in pro-inflammatory phase (RAW264.7 M1 subtypes). The regulation of several α2,6 sialyltransferase genes was coincident with the regulation of the α2,6 sialic acid on the two cell lines. The lectin response and glycosyltranferase gene expression confirmed that α2,6 sialic acid showed higher expression in the anti-inflammatory phase. This indicated that α2,6 sialic acid was a potential indicator for the anti-inflammatory response.
研究领域:
免疫学
·
分子生物学
·
细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Wanjun Jin,Yu Lu,Cheng Li,Meiyi Zou,Qinghui Chen,Lijing Nan,Ming Wei,Chengjian Wang,Linjuan Huang,Zhongfu Wang
Sialylated human milk oligosaccharides (SHMOs) possess unique biological activities. Qualitative and quantitative analyses of SHMOs at different lactation stages are limited by interference from neutral oligosaccharides, glycan structural complexity, and low detection sensitivity. Herein, our previously developed glycoqueuing strategy was improved and applied to enable an isomer-specific quantitative comparison of SHMOs between colostrum milk (CM) and mature milk (MM). A total of 49 putative structures were determined, including 1 α2,6-linked and 13 α2,3-linked isomers separated from seven newly discovered SHMO compositions. The content of most oligosaccharides was more than 50% lower in MM than in CM, and α2,3-sialylation was observed in 43.74% of SHMOs from CM and 22.95% of SHMOs from MM. Finally, the fucosylation level of the SHMOs increased from 16.45 to 22.28% with prolonged lactation. These findings provide the basis for further studies on the structure–activity relationship of SHMOs and a blueprint to improve infant formula.
研究领域:
生物化学
·
营养学
·
儿科医学
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DOI
PubMed
高影响因子
Plant Stress
·
2024
·
IF 6.8
Haiyang Chi,Shasha Bai,Chenxiao Chen,Yuying Bao,Xiaoxin Qu,Shitao Sun,Jiangpeng Pan,Xiushi Yang,Chunsheng Hou,Yanchun Deng
Squalene plays a crucial role in plant growth, development, and stress tolerance. To select the best reference genes (RGs) for expression profile analysis of genes involved in squalene biosynthesis in olives under stress tolerance, the expression stability of 22 candidate RGs across four tissues (root, stem, leaf, and fruit) and five representative cultivars under drought stress was assessed using six methods. Our study showed that, ubiquitin-conjugating enzyme1 ( UBC1 ) and 60S ribosomal protein L18-2-like ( 60S ) were the most stable RGs across five different cultivars and various tissues. Additionally, elongation factor 1-alpha-like ( EF1-α2) and UBC1 were the most stable RG in olives under drought stress. UBC1 was the appropriate RG for further study, qPCR analysis showed that five certain genes involved in the squalene biosynthesis exhibited significant differential expression under varying conditions. Specially, the expression level of squalene synthase (SQS) in the leaves was highest, and HPLC analysis showed that squalene content was the highest in leaves. Likewise, the expression levels of SQS and farnesyl diphosphate synthase ( FPPS ) in the leaves of the cultivar Arbosana and Chelmsford Lal were significantly higher than those of the other cultivars, respectively, and HPLC analysis also showed that squalene content was highest in the two cultivars. Interestingly, the amount of squalene increased dramatically in olive under drought stress, as lupeol synthase ( LUPS ) was significantly up-regulated. To date, this study firstly provided the comprehensive analysis of RGs related to squalene biosynthesis in various cultivars and tissues of olives under drought stress.
研究领域:
植物科学
·
分子生物学
·
胁迫生理学
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高影响因子
HORMONE AND METABOLIC RESEARCH
·
2020
·Vol. 52
·期 10
·755-763
·
IF 2.562
Wenge Zhang,Yu Qiao,Fulei Qi,Qingyi Shen,Ruqian Zhao,Xiaojing Yang
Zinc-α2-glycoprotein (ZAG), as an adipokine, plays an important role inlipid metabolism. However, its influence on whole gene expression profile inadipose tissue is not known. Under stress condition, how ZAG affects the lipidmetabolism is also unclear. Therefore, in this study ZAG systemic knockout (KO)mice were used as a model to reveal the genes expression profile in visceral fattissues of ZAG KO mice and wild-type mice by genome-wide microarray screening.Then dexamethasone (DEX) was used to explore the effect of ZAG deletion on bodyfat metabolism under stress. Our results showed that 179 genes weredifferentially expressed more than 1.5 times between ZAG KO mice and wild typemice, of which 26 genes were upregulated dramatically and 153 genes weresignificantly downregulated. Under DEX simulated stress, ZAG systemic knockoutin vivo resulted in a markedly decrease of triglycerides (TG) and nonesterifiedfatty acid (NEFA) content in in plasma. Similarly, for lipid catabolism, ZAG KOled to a significant increase of phosphorylated HSL (p-HSL) protein and a risingtendency of adipose triglyceride lipase (ATGL) protein relative to those of theDEX group. For lipid anabolism, fatty acid synthase (FAS) and adiponectinprotein expression in visceral fat rose notably in ZAG KO mice after DEXtreatment. In conclusion, ZAG knockout can affect the gene expression profile ofadipose tissue, reduce elevated TG and NEFA levels in plasma, and alter lipidmetabolism under DEX treatment. These findings provide new insights into themechanism of lipid metabolic disorders in response to stress.
研究领域:
分子生物学
·
内分泌学
·
代谢学
·
基因组学
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DOI
PubMed
高影响因子
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