ELECTROANALYSIS
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2021
·Vol. 34
·期 2
·263-274
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IF 3.223
Minling Ding,Qi Shu,Nan Zhang,Chaoren Yan,Huizhe Niu,Xiaoqian Li,Ping Guan,Xiaoling Hu
The amyloid-β peptide (1–42) (Aβ 42 ) can provide high diagnostic accuracy for the early diagnosis of Alzheimer's disease (AD). In this study, an immunosensor based on the peroxidase-like activity of heme-Aβ 42 was designed for Aβ 42 detection. The glassy carbon electrode (GCE) was electrodeposited Au particles (GCE/Au). The GCE/Au was modified using polythionine-methylene blue (PTH−MB), AuNPs, monoclonal antibody (anti-Aβ 42 ), and bovine serum albumin (BSA), successively. The GCE/Au/PTH−MB/AuNPs/anti-Aβ 42 /BSA could competitively recognize Aβ 42 and heme-Aβ 42 . The anchored heme-Aβ 42 showed strong electrocatalytic activity between H 2 O 2 and ferrocenemethanol. Under optimum conditions, we determined detection limits of 17.3 pM in PBS pH 7, 25.2 pM in serum, and 23.8 pM in saliva. The recovery ranged from 71.0 % to 117.8 %.
研究领域:
分析化学
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生物化学
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神经科学
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生物医学工程
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2015
·
IF 4.074
Cong Chen,Yan Meng,Sheng Li,Wenhua Wu,Chuanjun Liu,Xiaojuan Xu,Lina Zhang
A polysaccharide coded as PR-CA was extracted from Polyporus rhinoceros and determined to be a β-(1→3)- d -glucan with multiple branches. The weight-average molecular weights ( M w ) of PR-CA in dimethylsulfoxide (DMSO) and in water were determined with static light scattering (SLS) to be 3.57 × 10 5 and 1.79 × 10 7 , indicating existence of the single chains in DMSO and co-existence of single chains and aggregates in water. Moreover, the stiffness of single chains of PR-CA in water was directly visualized by atomic force microscopy (AFM) and transmission electron microscopy (TEM). The hollow structure of PR-CA nanofibers with width of 30–40 nm and length of ∼350 nm formed in the water/DMSO (9:1, v:v) was demonstrated by a fluorescent probe tetraphenylethylene (TPE) via aggregation-induced emission (AIE). The formation of PR-CA nanofibers was ascribed to the parallel aggregation of the extended PR-CA chains due to the hydrogen bonding and hydrophobic interaction. This work offered valuable results for promising applications of natural branched β-glucans in the biological fields of drug inclusion, delivery and disease diagnosis.
研究领域:
生物化学
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材料科学
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药学
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产品关联
DOI
PubMed
高影响因子
SYNTHESIS-STUTTGART
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2021
·Vol. 53
·期 14
·2435-2448
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IF 3.157
Xin Zhou,Qing Long,Dongwei Li,Jingru Gao,Qikai Sun,Shaozi Sun,Yong Su,Peng Wang,Wenjie Peng,Ming Li
Structurally defined and pure oligosaccharides corresponding to β-glucans have attracted great attention because of their potential properties as immunostimulating agents and as antigens of vaccine candidates. We herein describe a convergent synthesis of ready-to-conjugate tridecasaccharides composed of a β-1,3-glucan nonasaccharide backbone and a β-1,6-glucan tetrasaccharide branch. The assembly was achieved by employing trichloroacetimidate glycosylations and features the gram-scale preparation of the nonasaccharide backbone and installation of the tetrasaccharide branch involving orthoester rearrangement to the glycoside.
研究领域:
有机化学
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生物化学
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疫苗研究
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DOI
高影响因子
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
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2018
·
IF 3.679
Yu Zhang,Yudan Zhao,Zhuoyi Wang,Hao Gong,Liang Ma,Dongsheng Sun,Chen Yang,Yang Li,Biao Cheng,Robert B. Petersen,Fengchao Jiang,Gongping Liu,Kun Huang
Protein misfolding and aggregation are associated with amyloidosis. The toxic aggregation of amyloid-β 1–42 (Aβ42) may disrupt cell membranes and lead to cell death and is thus regarded as a contributing factor in Alzheimer's disease (AD). 1,4-naphthoquinone (NQ) has been shown to exhibit strong anti-aggregation effects on amyloidogenic proteins such as insulin and α-synuclein; however, its high toxicity and poor solubility limit its clinical application. Menadione sodium bisulfite (MSB, also known as vitamin K3), is used clinically in China to treat hemorrhagic diseases caused by vitamin K deficiency and globally as a vitamin K supplement. We hypothesized that MSB could inhibit amyloid formation since its backbone structure is similar to NQ. To test our hypothesis, we first investigated the effects of MSB on Aβ42 amyloid formation in vitro . We found that MSB inhibited Aβ42 amyloid formation in a dose dependent manner, delayed the secondary structural conversion of Aβ42 from random coil to ordered β-sheet, and attenuated the ability of Aβ42 aggregates to disrupt membranes; moreover, the quinone backbone rather than lipophilicity is esstial for the inhibitory effects of MSB. Next, in cells expressing a pathogenic APP mutation (Osaka mutation) that results in the formation of intraneuronal Aβ oligomers, MSB inhibited the intracellular aggregation of Aβ. Moreover, MSB treatment significantly extended the life span of Caenorhabditis elegans CL2120, a strain that expresses human Aβ42. Together, these results suggest that MSB and its derivatives may be further explored as potential therapeutic agents for the prevention or treatment of AD.
研究领域:
神经科学
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药理学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2025
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IF 8.2
Xuecheng Yang,Xiaoyu Zhang,Ge Yu,Yubin Ma,Xin Li,Yiyang Guo,Shuping Wang,Dan Ge,Changying Xue,Kun Jin,Bingbing Sun
Amyloid-β (Aβ) fibrillation is a spontaneous, thermodynamic process governed by nucleation and elongation. While many studies have explored the ability of engineered nanomaterials (ENMs) to modulate Aβ fibrillation, such as inhibitors, promoters, and dual-modulators, the key physicochemical property of ENMs that determines this behavior remains unclear. In this study, we developed a comprehensive library of ENMs with well-controlled physicochemical properties, including surface charges, morphologies, and hydrophilicity, to systematically investigate their effects on Aβ40 fibrillation. We identified hydrophilicity as the primary determinant of ENM-mediated modulation, rather than surface charge or morphology. Thioflavin T (ThT) kinetics assays indicated that hydrophilic ENMs exhibited bidirectional modulation, both promoting and inhibiting fibrillation depending on concentration. This bidirectional effect results from a competition between accelerated nucleation and decelerated elongation. While hydrophobic ENMs exhibited only unidirectional inhibition from the initial nucleation phase, two-dimensional-NMR (2D-NMR) mechanism studies indicated that this difference resulted from specific interactions with Aβ40 residues. Hydrophilic ENMs targeted hydrophilic residues involved in elongation, including Arginine R5, Glycine G9, G25, G33, G37, and G38, Lysine K28, and Alanine A30, while hydrophobic ENMs bound to hydrophobic residues critical for nucleation, such as I31. These findings provide mechanistic insight into NP–peptide interactions and lay a foundation for the rational design of nanomaterials to modulate amyloid fibrillation.
研究领域:
纳米技术
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生物化学
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神经科学
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材料科学
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生物物理学
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产品关联
DOI
PubMed
高影响因子
BMC PLANT BIOLOGY
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2022
·Vol. 22
·期 1
·1-15
·
IF 5.26
Wang Haitang,Zhou Xuesong,Liu Chuchu,Li Weixi,Guo Wangzhen
Background In eudicots, germination begins with water uptake by the quiescent dry seed and is greatly related to the permeability of micropyle enriched callose layers. Once imbibition starts, seeds undergo a cascade of physiological, biochemical, and molecular events to initiate cellular activities. However, the effects of callose on water uptake and following seed metabolic events during germination are largely unknown. Cotton ( Gossypium hirsutum ) is a eudicot plant with natural fiber and edible oil production for humans. Here, we addressed this question by examining the role of GhGLU19 , a gene encoding β-1,3-glucanase, in cotton seed germination. Results GhGLU19 belongs to subfamily B and was expressed predominately in imbibed seeds and early seedlings. Compared to wild type, GhGLU19 -suppressing and GhGLU19 -overexpressing transgenic cotton lines showed the higher and lower seed germination percentage, respectively. Callose was enriched more at inner integument (ii) than that in embryo and seed coat in cotton seeds. In GhGLU19 -suppressing lines, callose at ii of cotton seeds was greatly increased and brought about a prolonged water uptake process during imbibition. Both proteomic and transcriptomic analysis revealed that contrary to GhGLU19 -overexpressing lines, the glycolysis and pyruvate metabolism was decreased, and abscisic acid (ABA) biosynthesis related genes were downregulated in imbibed seeds of GhGLU19 -suppressing lines. Also, endogenous ABA was significantly decreased in GhGLU19 -suppressing line while increased in GhGLU19 -overexpressing line. Conclusions Our results demonstrate that suppression of GhGLU19 improves cotton seed germination via accumulating callose of inner integument, modulating glycolysis and pyruvate metabolism, and decreasing ABA biosynthesis. This study provides a potential way for improving germination percentage in cotton seed production, and other eudicot crops.
研究领域:
植物学
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遗传学
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分子生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Liang Wei,Keyu Qu,Jun Huang,Zhe Li,Xianyi Ning,Xiande Yang,Meng Li,Hongxi Zhang,Jing Yang
Lignin, one of the most prevalent organic polymers on Earth, is predominantly produced as a byproduct in the pulp and paper industry and is frequently disposed of as waste, posing substantial environmental challenges. The carbon‑carbon (C C) bonds in lignin are notably stable, complicating catalytic activation and cleavage. This study presents a significant advancement in the visible-light-driven cleavage of lignin's recalcitrant C C bonds (specifically the β-1 type) utilizing mixed-phase TiO 2 (P25) under ambient conditions. In contrast to conventional approaches that emphasize C O bond scission, the P25 catalyst achieves a 93 % conversion rate and 87 % selectivity towards benzaldehyde from 1,2-diphenylethanol without the use of noble metals or complex modifications. Density Functional Theory (DFT) calculations reveal that the rutile {110}/anatase {101} interface lowers the energy barrier for ·O₂ − generation, corroborating experimental selectivity trends. The synergy between these facets facilitates interfacial charge transfer, generating superoxide radicals (·O₂ − ) as the dominant reactive species for C C activation. When applied to native lignin, β-1 and β-β bonds have been completely broken, with over 70 % of β-5 bonds also cleaved. In contrast, only 3 % of β-O-4 bonds were broken, validating scalability beyond model compounds. This work provides a solar-driven, metal-free route to valorize lignin waste, addressing both environmental and energy challenges.
研究领域:
环境科学
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材料科学
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有机化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Zhen Qin,Si Lin,Yongjun Qiu,Qiming Chen,Yin Zhang,Jiachun Zhou,Liming Zhao
Immobilization of enzymes is an essential strategy with outstanding prospects in biocatalytic processes. Nontoxic, inexpensive immobilized enzyme approach is especially important for food enzymes. We here demonstrate that a carbohydrate-binding module family 56 domain (CBM56-Tag) mediates the immobilization of fusion enzymes with the curdlan (β-1,3-glucan) particle support, thereby enabling the one-step immobilization-purification of target enzymes. CBM56-Tag exhibits an immunoglobulin-like β-sandwich fold, which can be adsorbed by curdlan via hydrogen bond-mediated binding. The maximum adsorption capacity of a fusion chitosanase (CBM56-GsCsn46A) on curdlan is 50.72 mg/g. The immobilized enzyme could be directly used in the packed-bed reactor. This immobilization strategy utilizes a natural polysaccharide without any treatment, avoiding the negative environmental effects. Moreover, the one step immobilization-purification simplifies the purification step, which reduces the use of chemicals. Our study provides a nontoxic and inexpensive immobilization strategy for the biocatalytic reaction in food industry.
研究领域:
生物化学
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食品科学
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酶工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Keyu Lin,Zhongyu Shi,Zheyi Zhang,Yunfan Wei,Sibao Wan,Haiyan Gao,Zhen Qin
β-1,3-1,4-Glucanases have attracted significant interest because of their potential applications in various food industries. Glycoside hydrolase (GH) family 148 β-1,3-1,4-glucanases exhibit excellent thermal stability and a unique catalytic mechanism, making them particularly promising for certain food processing applications. This study investigated the module architecture enzymatic properties, catalytic mechanisms, and potential applications of a multi-domain β-1,3-1,4-glucanase (EngU) from GH family 148. The overall structure of EngU comprises three independently folding domains, namely the GH148 catalytic domain, CBM4, and the C-terminal domain. The insertion of CBM4 causes the catalytic domain of EngU to be non-contiguous in sequence. The optimum reaction conditions for EngU have been found to be pH 6.0 and 90 °C, demonstrating relatively high thermostability. EngU is an atypical β-1,3-1,4-glucanase, predominantly cleaves the β-1,3 glycosidic bonds in β-1,3-1,4-glucan. Malt saccharification experiments revealed that adding EngU (80 U/g malt) can decreased the viscosity of mash by 6.85 % and the filtration time by 28.83 %. Furthermore, EngU was found to effectively hydrolyze oat bran, producing β-glucooligosaccharides, with the main hydrolysis products being trisaccharide and disaccharide. These results demonstrate the unique module architecture and the application potential of GH family 148 β-1,3-1,4-glucanases, providing valuable insights and resources regarding this category of glycoside hydrolases.
研究领域:
生物化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2025
·
IF 12.5
Bingqian Nie,Jianle Chen,Wenkang Wang,Tong Cao,Xingqian Ye,Shiguo Chen,Zhiqiang Hou
Ganoderma lucidum samples from space-mutated strains transported by China's “Shenzhou” recoverable satellites, along with traditionally cultivated counterparts, were selected for this study. Polysaccharides from Ganoderma lucidum were extracted using a two-step sequential method integrating hot water and alkaline extractions. Liquid chromatography-mass spectrometry (LC-MS) was employed to characterize polysaccharide structures (monosaccharide composition and glycosidic linkages) and profile terpenoids, assessing the impact of space mutation. Results showed a significant increase ( p < 0.05) in polysaccharide and terpenoid contents, with polysaccharide yield rising from 178.59 % to 265.38 %. Space-mutated polysaccharides exhibited higher glucose content, altered monosaccharide composition, lower molecular weight, and an enriched β-1,3-glucan structure. They also demonstrated enhanced anti-inflammatory activity, underscoring the functional benefits of space-induced modifications. This study presents the first comprehensive analysis of structural alterations in Ganoderma lucidum polysaccharides induced by space mutation. It provides valuable scientific insights into the space breeding of medicinal plants and suggests that space mutation technology could serve as a promising strategy for developing functional foods from Ganoderma lucidum .
研究领域:
植物学
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药理学
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食品科学
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产品关联
DOI
PubMed
高影响因子
ACS Catalysis
·
2017
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IF 10.614
Tingting Hou,Nengchao Luo,Hongji Li,Marc Heggen,Jianmin Lu,Yehong Wang,Feng Wang
Selective cleavage of C–C bonds is pursued as a useful chemical transformation method in biomass utilization. Herein, we report a hybrid CuOx/ceria/anatase nanotube catalyst in the selective oxidation of C–C bonds under visible light irradiation. Using the lignin β-1 model as a substrate offers 96% yields of benzaldehydes. Characterization results by high angle annular dark field scanning transmission electron microscopy (HAADF-STEM) and energy-dispersive X-ray spectroscopy element (EDX) mapping reveal that CuOx clusters are highly dispersed on the exposed anatase surface as well as on the nanosized ceria domains. In-depth investigations by Raman and ultraviolet visible diffuse reflectance spectra (UV−vis DRS), together with density functional theory (DFT) calculations, further verify that the CuOx clusters present on the ceria domains increase the concentration of surface defects (Ce3+ ions and oxygen vacancies) and accordingly improve the photocatalytic activity (Yang character); the CuOx clusters decorating on anatase suppress the side reaction (oxy-dehydrogenation without C–C bond cleavage) because of an upward shift in the valence band (VB) edge of anatase (Yin character). Mechanism investigation indicates hydrogen abstraction from β-carbon by photogenerated holes is a vital step in the conversion.
研究领域:
化学
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材料科学
·
催化反应
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Tianxiang Zhu,Songlin Liu,Yu Gao,Xiongjie Sun,Haiming Hu,Zhigang Zhang,Wenwen Zhang,Lei Xiong,Hongtao Liu
Endoglucanases may not only act on β-1,4-linkages in β-glucan but also target d-glucose in glucomannan, which can expand their utility in the preparation of prebiotics. In this study, a highly efficient endoglucanase (BlGH5) from Bacillus licheniformis BJ2022 was expressed and characterized. BlGH5 exhibited the highest activity at pH 7.0 and 60 °C. It maintained over 80% activity at pH 4.0–7.0 and 30–60 °C. BlGH5 specifically cleaved β-1,4-glycosidic bonds linked to d-glucose. Site-directed mutagenesis results suggested that Arg91, Asn167, Glu168, Trp206, His228, Tyr230, Ser263, and Trp290 are key residues for its binding and catalytic activity. Moreover, BlGH5 displayed high activity against konjac glucomannan (KGM), indicating that BlGH5 could be used to prepare low-molecular-weight konjac glucomannan (KGM-L). Based on the physicochemical properties of KGM and KGM-L, KGM-L was characterized by reduced molecular weight and viscosity. Fecal fermentation experiments demonstrated that KGM and KGM-L could promote the production of short-chain fatty acids (SCFAs). Still, KGM-L was more conducive to the growth of the gut probiotics. In conclusion, we identified an endoglucanase for the degradation of KGM. Results indicated that KGM-L would have superior prebiotic effects. Thus, our study provides a basis for the future development and application of KGM-L as a prebiotic.
研究领域:
酶学
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食品科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）