ENVIRONMENTAL POLLUTION
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2017
·
IF 5.099
Qian Liu,Wentao Shao,Chunlan Zhang,Cheng Xu,Qihan Wang,Hui Liu,Haidong Sun,Zhaoyan Jiang,Aihua Gu
Organochlorine pesticides (OCPs) can persistently accumulate in body and threaten human health. Bile acids and intestinal microbial metabolism have emerged as important signaling molecules in the host. However, knowledge on which intestinal microbiota and bile acids are modified by OCPs remains unclear. In this study, adult male C57BL/6 mice were exposed to p, p’-dichlorodiphenyldichloroethylene (p, p’-DDE) and β-hexachlorocyclohexane (β-HCH) for 8 weeks. The relative abundance and composition of various bacterial species were analyzed by 16S rRNA gene sequencing. Bile acid composition was analyzed by metabolomic analysis using UPLC-MS. The expression of genes involved in hepatic and enteric bile acids metabolism was measured by real-time PCR. Expression of genes in bile acids synthesis and transportation were measured in HepG2 cells incubated with p, p’-DDE and β-HCH. Our findings showed OCPs changed relative abundance and composition of intestinal microbiota, especially in enhanced Lactobacillus with bile salt hydrolase (BSH) activity. OCPs affected bile acid composition, enhanced hydrophobicity , decreased expression of genes on bile acid reabsorption in the terminal ileum and compensatory increased expression of genes on synthesis of bile acids in the liver. We demonstrated that chronic exposure of OCPs could impair intestinal microbiota; as a result, hepatic and enteric bile acid profiles and metabolism were influenced. The findings in this study draw our attention to the hazards of chronic OCPs exposure in modulating bile acid metabolism that might cause metabolic disorders and their potential to cause related diseases in human.
研究领域:
毒理学
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微生物学
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代谢组学
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环境健康
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DOI
PubMed
高影响因子
MOLECULES
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2026
·Vol. 31
·期 17
·2989
·
IF 5.1
Dongdong Shi,Guiqin Yan
Herein, a highly selective and sensitive fluorescent sensing system is developed for the accurate quantitative determination of γ-Hexachlorocyclohexane (γ-HCH, Lindane). With carboxyl-functionalized CdSe/ZnS core–shell quantum dots (QDs) as the substrate, 3-Aminophenylboronic acid (3-APBA) is covalently conjugated onto the quantum dot surface via an amidation reaction. Systematic characterizations are used to confirm the successful fabrication of the CdSe/ZnS-COOH@3-APBA fluorescent probe, which exhibits excellent luminescence properties and superior colloidal dispersion stability. Under optimal experimental conditions, an increased γ-HCH concentration induces gradual attenuation of the probe fluorescence intensity. A favorable linear correlation is achieved within 10–140 nM, and the limit of detection is 2.62 nM. Mechanistic studies reveal that γ-HCH binds to 3-APBA on the probe surface via halogen bonding to form non-fluorescent ground-state association complexes, thereby triggering static quenching of the probe. This method can effectively distinguish α-, β-, and δ-hexachlorocyclohexane isomers. With the merits of simple operation, high sensitivity, excellent stability, and strong anti-interference capability, it provides a new strategy for the rapid screening and accurate quantification of γ-HCH residues in environmental and food matrices.
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2025
·
IF 8
Xiang Shen,Zheshan Duan,Xike Tian,Hongtao Zheng,Dongsheng Wang,Xubo Gao
Inland freshwater is an essential resource for human survival and development; however, the occurrence of organochlorine pesticides (OCPs) in the largest freshwater lake in northern China remains largely unknown. Among the 24 OCPs detected in the lake sediments, hexachlorocyclohexanes (HCHs) and dichlorodiphenyltrichloroethanes (DDTs) were predominant compounds. The most abundant isomers were β-HCH and p,p'-DDT, reflecting their extensive use in agriculture and aquaculture. The Fuhe River, a critical channel for agricultural irrigation and domestic sewage, was identified as the primary pathway for transporting OCPs from upstream sources into Baiyang Lake. The residual OCPs in surface sediments were higher than those in deeper sediments, indicating the recent input of lindane, dicofol and dieldrin. Especially, the α-HCH/γ-HCH ratios (0–1.8) in the sediment cores, indicated a mixed historical input of both lindane and technical HCHs. The o,p'-DDT/p,p'-DDT > 0.5 in the bottom sediments were attributed to the earlier use of technical DDT. Notably, the DDTs in the upstream section of the Fuhe River, with high ERM values, posed a significant ecological risk, whereas the levels of DDD, DDE, and chlordane in the sediments were below the ERL values, indicating low potential ecological risks. The sediment assessment indicated potential ecological risks from HCHs and dieldrin in the residential areas. In contrast, the sediment-associated surface water posed no significant carcinogenic or non-carcinogenic risks to humans.
研究领域:
环境科学
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生态毒理学
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水文学
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污染研究
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沉积物化学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2019
·
IF 6.393
Xia Zhou,Qian Zhao,Guangqiang Liu,Weiping Cai
Active capture and ultrasensitive SERS-based detection of hexachlorocyclohexane (HCH) has been a challenging task. A novel strategy based on 4-mercaptophenylboronic acid (4-MPBA) surface modification on Au nanosheets-built hollow sub-microcubes (ANHCs) was proposed to capture HCH pesticides effectively via Suzuki cross coupling reaction. The ANHCs structure composed of numerous intertwined nanosheets was firstly prepared by Au nanocrystals heteroepitaxial growth on AgCl cubes. Then, the 4-MPBA molecules were selected to modify on the surface of ANHCs for SERS-based detection of HCH. The SERS results proved that active capture and ultrasensitive detection of HCH could be implemented, and the limit of detection is 0.3 ppb. Besides, 4-MPBA modified substrate show high reproducibility and sensitivity than unmodified one. Moreover, the characteristic peak intensity ratio I HCH /I 4-MPBA presents a good linear relationship with the logarithm of a certain concentrations, and achieve SERS-based quantitative detection of HCH. In addition, this strategy has been successfully used for the detection and differentiation of mixed HCH isomers in water samples. The surface modification strategy on high performance SERS substrate provides a good route for the highly ultrasensitive detection and active capture of HCH, and paves a way for the detection of organochlorine pesticide residues in water samples.
研究领域:
分析化学
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环境科学
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纳米技术
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DOI
高影响因子
ANALYST
·
2015
·Vol. 141
·期 1
·243-250
·
IF 4.107
Mingxue Wu,Gang Chen,Ping Liu,Weihong Zhou,Qiong Jia
A novel hybrid material incorporating porous aromatic frameworks and an ionic liquid, 1-(triethoxy silyl)propyl-3-aminopropyl imidazole hexafluorophosphate, was prepared as solid-phase microextraction coating and employed for the extraction of organochlorine pesticides. Combining the advantages of porous aromatic frameworks and an ionic liquid, the fiber exhibited a high adsorption capacity for organochlorine pesticides. Under optimized experimental conditions, enhancement factors of 247–1696 were obtained with good linearity in the range of 1–500 μg L−1. The detection limits and quantification limits were determined to be in the range of 0.11–0.29 μg L−1 and 0.35–0.93 μg L−1. The relative standard deviations for six replicates of organochlorine pesticides were in the range of 4.4%–7.2% and 5.7%–10.1% for one fiber and fiber-to-fiber, respectively. By coupling with a gas chromatography–electron capture detector, the novel fiber was successfully used for the determination of organochlorine pesticides in juice and milk samples with recoveries of 76.1%–121.3%.
研究领域:
分析化学
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环境科学
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DOI
PubMed
高影响因子
Scientific Reports
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2017
·Vol. 7
·期 1
·1-11
·
IF 4.259
Liu Qian,Wang Qihan,Xu Cheng,Shao Wentao,Zhang Chunlan,Liu Hui,Jiang Zhaoyan,Gu Aihua
p,p’-dichlorodiphenyldichloroethylene (p, p’-DDE) and β-hexachlorocyclohexane (β-HCH) were two predominant organochlorine pesticides (OCPs) metabolites in human body associated with disorders of fatty acid metabolism. However, the underlying mechanisms have not been fully clarified. In this study, adult male C57BL/6 mice were exposed to low dose of p, p’-DDE and β-HCH for 8 wk. OCPs accumulation in organs, hepatic fatty acid composition, tricarboxylic acid cycle (TCA) metabolites and other metabolite profiles were analyzed. Expression levels of genes involved in hepatic lipogenesis and β-oxidation were measured. Mitochondrial function was evaluated in HepG2 cells exposed to OCPs. High accumulation of p, p’-DDE and β-HCH was found in liver and damaged mitochondria was observed under electron microscopy. Expression of genes in fatty acid synthesis increased and that in mitochondrial fatty acid β-oxidation decreased in OCPs treatment groups. OCPs changed metabolite profiles in liver tissues, varied hepatic fatty acid compositions and levels of several TCA cycle metabolites. Furthermore, MitoTracker Green fluorescence, ATP levels, mitochondrial membrane potential and OCR decreased in HepG2 cells exposed to OCPs. In conclusion, chronic exposure to OCPs at doses equivalent to internal exposures in humans impaired mitochondrial function, decreased fatty acid β-oxidation and aggravated disorders of fatty acid metabolism.
研究领域:
毒理学
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药理学
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分子生物学
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细胞生物学
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DOI
PubMed
高影响因子
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