NEUROCHEMICAL RESEARCH
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2016
·Vol. 41
·期 11
·2890-2903
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IF 2.472
Han Jun,Zhang Hai,Wang Sheng,Zhou Jun,Luo Yi,Long Li-Hong,Hu Zhuang-Li,Wang Fang,Chen Jian-Guo,Wu Peng-Fei
Sulfhydryl compounds such as dithiothreitol (DTT) and β-mercaptoethanol (β-ME) are widely used as redox agents. Previous studies in our group and other laboratory have reported the effect of sulfhydryl compounds on the function of glutamate receptor, including plasticity. Most of these findings have focused on the N -methyl- d -aspartic acid receptor, in contrast, very little is known about the effect of sulfhydryl compounds on α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor (AMPAR). Here, we observed that DTT (100 μM), β-ME (200 μM) and l -cysteine (200 μM) significantly elevated the surface expression of AMPARs via reducing their palmitoylation in rat hippocampal slices in vitro . Increased surface stability of AMPARs was not be correlated with the altered redox status, because the chemical entities containing mercapto group such as penicillamine (200 μM) and 2-mercapto-1-methylimidazole (200 μM) exhibited little effects on the surface expression of AMPARs. Computing results of Asp-His-His-Cys (DHHC) 3, the main enzyme for palmitoylation of AMPARs, indicated that only the alkyl mercaptans with chain-like configuration, such as DTT and β-ME, can enter the pocket of DHHC3 and disrupt the catalytic activity via inhibiting DHHC3 auto-palmitoylation. Collectively, our findings indicate a novel redox-independent mechanism underlay the multiple effects of thiol reductants on synaptic function.
研究领域:
神经科学
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药理学
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细胞生物学
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Advanced Healthcare Materials
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2025
·
IF 9.6
Jiamei Fu,Mengqiu Quan,Haiquan Sun,Junyi Zhou,Qingyi Xie,Minghui Cui,Xiaolin Wang,Fenglong Li,Jin Zhu,Jing Chen
In the field of sports medicine, especially in high-intensity sports such as football, basketball, and skiing, ligament injuries are extremely common. However, current artificial ligaments face multiple critical limitations, including suboptimal biocompatibility, limited host tissue integration, insufficient mechanical durability to withstand physiological stresses, and inadequate durability during long-term cyclic loading. To address these challenges, 1-(2-Hydroxyphenyl)-3-phenyl-2-propenone (HCC), which shared structural similarity with naturally occurring chalcones, is employed as the raw material for the chain extender, combined with HMDI and polycaprolactone diol (PCL diol), to synthesize a novel polyurethane (HCMPU). The results showed that the maximum stress of HCMPU-3 reached 42.1 MPa, with an elongation at break of up to 710%. It also demonstrated outstanding durability, maintaining structural integrity without significant degradation after 5000 loading cycles. Additionally, during the initial implantation period, its stability in an enzymatic environment can provide strong support to the human body before new tissue regeneration, making it a promising candidate for an ideal artificial ligament. Moreover, the favorable biocompatibility of HCMPUs is demonstrated through cytotoxicity testing and rat pathological staining sections. Therefore, this work demonstrated the potential of HCMPU to overcome key limitations in current artificial ligaments and advance the development of next-generation materials for ligament repair.
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FOOD RESEARCH INTERNATIONAL
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2025
·
IF 8
Yuan Tang,Xiaojuan Lei,Yan Liu,Mengcong Liu,Lin Lei,Kaihong Zeng,Jian Ming
This study investigated interactions between capsaicin (CAP), piperine (PIP), allicin (ALL) and grass carp myofibrillar protein (MP) regarding protein conformational changes. While the bioactivities of these pungent compounds are recognized, a systematic understanding of their diverse binding patterns and consequent conformational remodeling of MP remains limited. The binding patterns and regulatory mechanisms were systematically evaluated through multispectral analysis, physicochemical characterization, and molecular docking simulations. Results demonstrated that CAP binding coincided with MP aggregation, increased surface hydrophobicity, and elevated random coil content, alongside the weakest binding energy (−6.9 kcal/mol). These effects were consistent with a binding pattern dominated by hydrophobic interactions and dynamic quenching. PIP binding correlated with conformational loosening and maintained colloidal dispersibility but limited stabilization, associated primarily with hydrogen bonding and static quenching (K q > 9.0 × 10 12 L/(mol·s)). ALL binding at higher concentrations corresponded to conformational compaction, increased α-helix/β-sheet content, and enhanced structural stability, with the strongest binding affinity (−19.82 kcal/mol). These changes aligned with a mechanism involving covalent bonding synergized by hydrogen/hydrophobic interactions. These findings provide molecular-scale insights into pungent spice-MP interactions, offering strategies for optimizing spicy food systems.
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Current Protocols
·
2025
·Vol. 5
·期 10
·e70241
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IF 2.2
Yiming Zhao,Jingjun Hong
Chromatin remodeling is a process of regulating gene expression levels by altering chromatin structure, facilitated by chromatin remodeling factors. ISWI (Imitation SWItch), a type of chromatin remodeling factor, plays a crucial role in epigenetic regulatory processes, such as cell differentiation and development. It primarily achieves remodeling effects by promoting the direct sliding of nucleosomes and their relocation on DNA. To study the function and structural characteristics of ISWI, this basic protocol describes a simple and rapid procedure for the large-scale expression and purification of soluble Ct ISWI 77-1038 and Ct ATPase 77-722 proteins in Escherichia coli ( E. coli ). The procedure provides high yields (∼20 mg) and is followed by in vitro measurement of ATP hydrolysis activity. © 2025 Wiley Periodicals LLC. Basic Protocol : Expression, purification, and ATP hydrolysis activity measurement of Ct ISWI and Ct ATPase
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FOOD CHEMISTRY
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2025
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IF 9.8
Amel Thanina Amrouche,Minghao Xu,Shengyang Ji,Chen Qi,Nesma S. Abdelaty,Baiyi Lu
Edible flowers are valued for their bioactive, nutritional, and aesthetic properties. This study explored the 3D printability of five edible flowers and identified the essential role of their native biopolymers through integrated analyses of composition, functionality, viscoelasticity, and structure. Heat treatment significantly enhanced printing precision in pectin- and protein-rich gels. The most substantial improvement occurred in pectin-rich roselle (59.96 % to 99.81 %) and protein-rich lotus (90.63 % to 96.25 %). Higher printability was linked to biopolymer-network rearrangement via hydrogen and disulfide bonds and electrostatic interactions, which reduced free water and lowered cellulose crystallinity (up to 11.00 %). These changes improved gel network flexibility, increased storage modulus, and improved microstructural regularity, resulting in smoother layer deposition. In contrast, cellulose-rich gels lacking sufficient pectin and protein underwent recrystallization (4.84 %) and densification after heating, reducing viscoelasticity and printability. These findings provide mechanistic insights for optimizing edible-flower gels for 3D food printing.
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Horticulturae
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2025
·Vol. 11
·期 11
·1292
·
IF 3
Hu Zhao,Xiaopu Shi,Zilu Zhao,Juan Wang,Baul Ko,Jong Hyang Bae,Yang Gyu Ku,Ho Cheol Kim,Chul Min Kim
Young leaves and sprouts ofToona sinensisare widely consumed throughout Asia. However, they are highly perishable, resulting in a short shelf life and limited marketability. Low-temperature storage can extend shelf life but is energy-intensive, restricting large-scale use. Salicylic acid (SA) exhibits preservative properties, representing a promising alternative. In this study, we investigated the effects of 250 μmol SA treatment on postharvestT. sinensisbuds and leaves stored at 20 °C for 7 days and compared it to low-temperature storage (4 °C). SA treatment delayed wilting, reduced water loss, suppressed total soluble solid accumulation, and inhibited active oxygen free radical and malondialdehyde production compared to the untreated controls at room temperature while outperforming low-temperature storage. The treated buds also maintained higher antioxidant enzyme activity and preserved non-enzymatic antioxidant compounds, including ascorbic acid, polyphenols, and flavonoids. Although total amino acid content steadily increased under SA treatment, individual amino acids greatly fluctuated. SA treatment reduced nitrate reductase and glutamate dehydrogenase activities, as well as NO2•−levels, indicating its effects on nitrogen metabolism. Our findings indicate that SA improves the postharvest quality ofT. sinensisbuds during room-temperature storage, providing a theoretical basis for applying SA as a storage agent forT. sinensisbuds.
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CARBOHYDRATE POLYMERS
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2025
·
IF 12.5
Xindong Xu,Yongxin Teng,Kaiyang Shi,Xin-An Zeng,Zhong Han,Ji Ma,Wen Chen
Efficient, eco-friendly removal of starch granule-channel proteins (SGCP) to enhance modifier penetration has been challenging. Current methods using chemical/biological reagents (denaturants, alkalis, proteases) suffer from toxicity, long processing times, and non-reusability. Inspired by capillary electrophoresis for protein separation, this study successfully removed SGCP from maize starch within 30 min using an 8 kV/cm pulsed electric field (PEF). This method only requires direct treatment of starch aqueous suspension to remove SGCP, and it achieved 29.9 % protein removal efficiency (primarily SGCP, along with minor amounts of intrinsic and storage proteins), comparable to the protease method but significantly faster. The mechanism of PEF-induced SGCP migration was explained using an electrokinetic migration velocity model based on Debye-Hückel-Henry theory. Using oxidized starch as a case study, we demonstrated that SGCP removal improved modification by increasing starch channel pore volume and accessibility. This work introduces an efficient, green SGCP removal technique and provides new insights into how PEF enhances starch modification, offering significant implications for improving modified starch quality and meeting sustainability criteria.
研究领域:
食品科学与技术
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电动学
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绿色化学
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淀粉化学
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生物高分子改性
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PHYTOMEDICINE
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2025
·
IF 8.3
Anna Gan,Jinyu Wang,Dehong Zhang,Min Xie,Bo Wu,Tingxu Yan,Ying Jia
Background Acute lung injury (ALI) is a severe pulmonary condition characterized by inflammation and damage to the epithelial barrier of the lungs. Sanzi Yangqin Decoction (SZYQ) is a traditional therapeutic formula for lung diseases and has been shown to play a role in the regulation of ALI; however, the specific mechanisms by which it modulates the epithelial barrier remain unclear. Purpose This study aims to elucidate the specific mechanisms by which SZYQ regulates epithelial barrier injury in ALI. Methods We first identified the constituents for SZYQ in plasma through UHPLC-QTOF-MS. Subsequently, we utilized the GEO database in conjunction with network pharmacology to predict the mechanism by which SZYQ regulates epithelial barrier damage in ALI. An in vitro ALI epithelial barrier injury model was established using LPS-induced MLE-12 cells to evaluate the efficacy and mechanism of SZYQ. Additionally, an in vivo ALI model was created through intranasal LPS administration to validate the in vitro findings. To explore the key target, PCR array analysis was conducted on lung tissues, revealing that SZYQ functions by regulating CD40 in alveolar macrophages (AMs). Following this, clodronate liposomes were employed for AMs depletion, and CD40 ligand (CD40L) stimulation of MH-S and MLE-12 cells co-culture was utilized to verify the pharmacological effects of SZYQ both in vivo and in vitro. Results This study identified a total of 18 constituents for SZYQ in plasma. GEO database analysis results suggest that SZYQ may alleviate epithelial barrier damage in ALI by exerting anti-necroptotic effects. Both in vitro and in vivo results indicated that SZYQ inhibits the expression of key necroptosis proteins RIP1, RIP3, and MLKL, alleviates DRP1-mediated mitochondrial fission, regulates ROS and Ca 2+ release, promotes the proliferation and differentiation of type II alveolar epithelial cells (AT2), and thereby mitigates epithelial barrier damage in ALI. Following the depletion of AMs in vivo , the necroptosis of AT2, mitochondrial fission, and lung epithelial barrier injury in ALI mice were exacerbated; while the regulatory capacity of SZYQ on these factors was diminished. The co-culture experiments involving MH-S cells and MLE-12 cells demonstrated that SZYQ exerts an anti-necroptotic effect on AT2 by inhibiting the expression of CD40 on AMs. Additionally, SZYQ improved epithelial barrier function by promoting the proliferation and differentiation of AT2. Conclusions SZYQ improved epithelial barrier function in ALI by mediating the CD40 of AMs. Its mechanism of action involved inhibiting necroptosis in AT2, alleviating mitochondrial fission, and promoting the proliferation and differentiation of AT2.
研究领域:
中医中药
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呼吸病学
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药理学
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分子生物学
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炎症研究
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细胞死亡机制
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高影响因子
MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Sai Zhang,Zeyang Liu,Qinqin Gao,Yaping Wang,Jiayi Zhou,Qinglin Sheng
Malathion, a broad-spectrum organophosphorus insecticide widely used in pest control for medicinal herbs, poses risks to human health and ecological balance due to its potential residual toxicity. In this study, we report a green method for the synthesis of silver-nanoparticles modified covalent organic frameworks (AgNPs@COFs) based on static in-situ reduction at room temperature. The material was combined with a malathion aptamer to develop a highly sensitive electrochemical aptasensor. The ferrocene-labeled hairpin aptamer was used as a molecular recognition element, and the sensing performance was evaluated using a differential pulse voltammetry (DPV) system. Under optimized conditions, the sensor exhibited a linear detection range of 10–800 ng/L, a low detection limit of 8.2 ng/L (S/N = 3, Δ I = 0.01255–0.00774 C Mal , R 2 = 0.995), and excellent repeatability ( RSD < 2.0 %, n = 3). The proposed platform was successfully applied to quantify malathion in real herbal samples (e.g., panax ginseng , astragalus membranaceus, lycium barbarum , and lonicera japonica ), achieving recoveries of 98.32 %–101.92 % with corresponding RSDs of 1.25 %–3.28 % ( n = 3). The sensor is expected to be applied to the rapid screening of malathion residues in medicinal herbs.
研究领域:
环境分析化学
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纳米材料
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食品药品安全
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电化学传感器
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适配体技术
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Mengyuan Liu,Yunxiang Sun,Yiqun Huang,Junjian Miao,Keqiang Lai
This study investigated the effects and potential mechanism of soybean dietary fiber (SDF, 0–1.2 %, w /w) on the gel properties and fishy volatile compounds of low-salt silver carp surimi (1.25 % NaCl). The results showed that at an appropriate level (0.3 % or 0.6 %), SDF in surimi promoted the formation of hydrophobic interactions and disulfide bonds, facilitated the β-sheet transition, and served as a filler within the gel network, leading to a more compact and denser network of a surimi gel. Excessive SDF competed with proteins for water, leading to SDF aggregation and volume exclusion, thereby generating a denser but incomplete gel network. Compared with the control group, the strength of surimi gels with 0.3 % and 0.6 % SDF was increased by 11.92 % to 20.02 %, while the corresponding water-holding capacity increased to 85.83–86.49 % and the free water content decreased by 30.10–41.75 %. Moreover, the total content of fishy volatile compounds decreased by 7.29 % to 13.80 %. Correlation analysis revealed that the seven identified fishy volatile compounds (hexanal, heptanal, octanal, nonanal, decanal, 1-octen-3-ol, and 1-hexanol) were negatively correlated with gel strength, water-holding capacity, immobile water, and hydrophobicity of the protein surface.
研究领域:
食品科学
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食品化学
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蛋白质工程
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功能食品
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jihao Zuo,Ruopeng Lan,Jinting Cai,Long Chen,Dongyan Yang,Huayao Chen,Li Hao,Ximing Zhong,Xinhua Zhou,Hongjun Zhou
Soil salinization poses a global agricultural challenge with significant economic implications for salt-sensitive cucumber ( Cucumis sativus L. ) cultivation. To address this issue, we developed a proline-functionalized zein nano delivery system (BR@AM-Zein-Pro) through amide coupling of proline to aminated zein (AM-Zein), followed by brassinolide (BR) encapsulation to form self-assembled architectures. BR@AM-Zein-Pro exhibits sustained release for BR, thereby extending the phytohormone's duration efficacy. Furthermore, proline functionalization enhances BR@AM-Zein-Pro penetration through cucumber seed coats, thereby improving osmotic regulation capacity. Crucially, cucumber seeds treated with BR@AM-Zein-Pro exhibited a 90–116.67 % higher germination potential than untreated salt-stressed seeds. Following seven days of growth, cucumber seedlings from seeds treated with BR@AM-Zein-Pro exhibited significant increases in root and stem length of 39.91 % and 43.81 %, respectively, compared to untreated salt-stressed seedlings. At the mature plant stage, foliar application of BR@AM-Zein-Pro to salt-stressed plants significantly enhanced superoxide dismutase (SOD) and peroxidase (POD) activities by 98.64 % and 56.42 %, respectively, relative to the untreated control group. Additionally, malondialdehyde (MDA) content decreased by 53.14 %, proline content reduced by 72.95 %, electrolyte leakage rate dropped by 22.48 %, and chlorophyll content increased by 50.33 %. Mechanistically, BR@AM-Zein-Pro enhances cucumber salt tolerance through synergistic coordination of osmotic adjustment, antioxidant defense activation, and photosynthetic efficiency maintenance. It is important that BR@AM-Zein-Pro significantly alleviated the phytotoxicity from high BR concentrations (>0.25 mg/mL) by maintaining optimal therapeutic levels, resolving the trade-off between efficacy and safety. This nano delivery system provides an innovative strategy for enhancing crop salt tolerance, offering transformative potential for agricultural sustainability and global food security.
研究领域:
植物科学
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农业纳米技术
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胁迫生理学
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作物改良
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农业生物技术
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高影响因子
FOOD CONTROL
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2025
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IF 6.3
Jinyu Xu,Xue Hei,Mumin Zheng,Yunna Wang,Ning Xie,Xu Li,Xiaodan Wang,Jiaping Lv,Gang Lu,Xiaoyang Pang,Dongwei Yu,Shuwen Zhang
The deterioration of ultra-high-temperature (UHT) milk quality during shelf life such as protein hydrolysis, is closely related to the residual activity of heat-resistant protease from psychrotrophic bacteria. This study aimed to evaluate the effect of heat-resistant protease activity derived from psychrotrophic bacteria on the quality of whole milk. Different concentrations of heat-resistant protease from Aeromonas sp .were added to UHT milk, and the effect on milk quality was investigated during storage time at different temperatures. The results indicate that as the shelf life extended, the pH, stability, and fluorescence intensity of all samples gradually decreased, while the degree of hydrolysis, average particle size, precipitation, and zeta potential significantly increased. The results of SDS-PAGE indicated that κ -casein was preferentially hydrolyzed by proteases. Moreover, the degree of deterioration in UHT milk was correlated with the concentration of proteases. As the protease concentration in milk reached 0.08 U/mL, the samples reached the end of the shelf life when stored at 37°C within 28 d, while no significant deterioration was observed after 98 d at 4°C. The results of this study provide valuable evidence for controlling quality of UHT milk during the storage of UHT milk and extending its shelf life.
研究领域:
食品科学
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微生物学
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乳品技术
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高影响因子
危险品化学品经营许可证（带存储）