JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Zengting Niu,Guotong Sun,Mingtao Tan,Shanchun Yan,Dun Jiang
Heavy metals are prevalent environmental contaminants in pest habitats. This study investigates the tolerance of Hyphantria cunea larvae to β-cypermethrin under cadmium (Cd) stress. Exposure to Cd markedly enhanced larval tolerance to β-cypermethrin. The P450 enzyme was found to be crucial in triggering this cross-tolerance. Notably, Cd exposure significantly upregulated the expression of HcCYP333A29 and HcCYP6AE184 both before and after β-cypermethrin treatment. Silencing these genes reduced the β-cypermethrin tolerance in Cd-exposed larvae. Furthermore, overexpression of HcCYP333A29 and HcCYP6AE184 significantly improved the tolerance of Sf9 cells and Drosophila to β-cypermethrin. Silencing of HcDicer-1, a key gene in the miRNA synthesis pathway, significantly diminished the β-cypermethrin tolerance in Cd-treated larvae. The miR-2739 controlled overexpression of HcCYP333A29, while miR-2854 controlled HcCYP6AE184 overexpression, both of which were essential for β-cypermethrin cross-tolerance in H. cunea larvae under Cd exposure. Collectively, Cd exposure induces cross-tolerance to β-cypermethrin in H. cunea larvae through miRNA-mediated post-transcriptional regulation.
研究领域:
环境毒理学
·
分子生物学
·
昆虫学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Jian Zhu,Yunyan Lai,Yinhua Wu,Jiali Wang,Jiaqi Wei,Hanhong Xu
We screened the contact activity of 32 commercial essential oils (EOs) and their synergistic effect with β-cypermethrin against Blattella germanica. Results showed that the most effective EOs against B. germanica were from Illicium verum, Syzygium aromaticum, and Cinnamomum camphora, with LD50 values of less than 500 μg/insect. The most potent synergistic effects of β-cypermethrin on B. germanica were from Dysphania ambrosioides and Mentha canadensis. Both oils have a co-toxic factor of 133.33. The results of the major compound testing of the EOs showed that trans-anisaldehyde and thymol have the best insecticidal activity against B. germanica, with LD50 values of 141.30 and 138.61 μg/insect, respectively. The compounds with the best synergistic effect on β-cypermethrin were γ-terpinene and linalool at a concentration of 0.5%. The co-toxic factors for γ-terpinene and linalool were 150 and 133.33, respectively, which were similar to the synergistic effect observed with 2% piperonyl butoxide.
研究领域:
昆虫学
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植物化学
·
害虫管理
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DOI
PubMed
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2022
·
IF 6.76
Jiexiang Liu,Ruili Bai,Xiaoguang Zhang
The SB (DTAB, TTAB, CTAB, OTAB)-AP complex was obtained by modifying attapulgite (AP) with surfactant of SB (DTAB, TTAB, CTAB, OTAB). The structures were characterized by XRD, FT-IR, TGA/DTA and SEM. A new lamellar structure is formed by introducing surfactant. The basal spacing increases with the increase of alkyl chain length of cationic surfactants . Then, the beta-cypermethrin (BCT) composites of BCT/SB-AP, BCT/DTAB (TTAB, CTAB, OTAB)-SB-AP were prepared and investigated. The basal spacings of BCT composites were larger than those of SB-AP and the corresponding DTAB (TTAB, CTAB, OTAB)-AP. The existence of BCT and surfactants molecules into BCT composites was further confirmed. The BCT/DTAB (TTAB, CTAB, OTAB)-SB-AP have the smaller specific surface areas than DTAB (TTAB, CTAB, OTAB)-AP due to the increase of AP exfoliation in the presence of SB. Fabrication mechanism of BCT composites was put forward. Release behaviors of BCT were investigated and analyzed in different buffer solutions. The existence of mixed-surfactants influenced distinctly the release rates. BCT composites had the slow release property. The release behaviors were well fitted by pseudo second-order and parabolic diffusion models. This study provided a new approach to improve the utilization efficiency of pesticide and could be applied as a pesticide delivery system.
研究领域:
材料科学
·
农业科学
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DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Wenqi Jiang,Jing Gao,Zheng Cheng,Wangjing Zhai,Donghui Liu,Zhiqiang Zhou,Peng Wang
Pesticide residues most likely coexist with antibiotics due to the application of animal-based fertilizers in agriculture. In this study, the degradation and enantioselectivity of beta-cypermethrin in soil and chicken manure-amended soil were investigated. The effects of oxytetracycline on the soil microbial community were also estimated. The results showed that the half-life of beta-cypermethrin in the soil was 16.9 days and that the (+)-enantiomer was degraded preferentially in both pairs of enantiomers. The metabolites cis/trans-DCCA(3-(2′,2′-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid) and 3-PBA (3-Phenoxybenzoic acid) were detected. The trans-DCCA concentrations ranged from 0.094 to 0.120 mg/kg, which were higher than the concentrations of cis-DCCA (0.091–0.120 mg/kg) and 3-PBA (0.022–0.061 mg/kg). In the presence of oxytetracycline, beta-cypermethrin degradation was inhibited slightly, while the enantioselectivity was not affected. Oxytetracycline increased the enrichment and persistence of the metabolites. Addition of chicken manure decreased the cis-DCCA residue levels in the soil and alleviated the effect of oxytetracycline; however, chicken manure increased the accumulation and persistence of 3-PBA. In addition, oxytetracycline perturbed the structure of the soil microbial community. The abundance of Proteobacteria increased, while the abundances of Firmicutes and Actinobacteria decreased. These changes might affect the biodegradation of beta-cypermethrin and its metabolites. Combined pollution with antibiotics should be considered for its potential impact on pesticide residues.
研究领域:
环境科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Chaojin Si,Hongjun Yang,Xiaoyan Wang,Qiaoxin Wang,Min Feng,Huayue Li,Yuqi Feng,Jiayuan Zhao,Ying Liao
Beta-cypermethrin (β-CYP) consists of four chiral isomers, acting as an environmental estrogen and causing reproductive toxicity, neurotoxicity, and dysfunctions in multiple organ systems. This study investigated the toxic effects of β-CYP, its isomers, metabolite 3-phenoxybenzoic acid (3-PBA), and 17β-estradiol (E2) on HTR-8/SVneo cells. We focused on the toxic mechanisms of β-CYP and its specific isomers. Our results showed that β-CYP and its isomers inhibit HTR-8/SVneo cell proliferation similarly to E2, with 100 μM 1S-trans-αR displaying significant toxicity after 48 h. Notably, 1S-trans-αR, 1R-trans-αS, and β-CYP were more potent in inducing apoptosis and cell cycle arrest than 1R-cis-αS and 1S-cis-αR at 48 h. AO/EB staining and flow cytometry indicated dose-dependent apoptosis in HTR-8/SVneo cells, particularly at 100 μM 1R-trans-αS. Scratch assays revealed that β-CYP and its isomers variably reduced cell migration. Receptor inhibition assays demonstrated that post-ICI 182780 treatment, which inhibits estrogen receptor α (ERα) or estrogen receptor β (ERβ), β-CYP, its isomers, and E2 reduced HTR-8/SVneo cell viability, whereas milrinone, a phosphodiesterase 3 A (PDE3A) inhibitor, increased viability. Molecular docking studies indicated a higher affinity of β-CYP, its isomers, and E2 for PDE3A than for ERα or ERβ. Consequently, β-CYP, its isomers, and E2 consistently led to decreased cell viability. Transcriptomics and RT-qPCR analyses showed differential expression in treated cells: up-regulation of Il24 and Ptgs2 , and down-regulation of Myo7a and Pdgfrb , suggesting the PI3K-AKT signaling pathway as a potential route for toxicity. This study aims to provide a comprehensive evaluation of the cytotoxicity of chiral pesticides and their mechanisms.
研究领域:
毒理学
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生殖生物学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Fang Liu,Jie Bai,Wenkang Huang,Fuhou Li,Wencan Ke,Yixin Zhang,Dongmei Xie,Bo Zhang,Xusheng Guo
In this study, a novel beta-cypermethrin (beta-cyp)-degrading strain Lactobacillus pentosus 3–27 (LP3–27) was screened from beta-cyp-contaminated silage. The strain could degrade 96% of beta-cyp (50 mg/L) in MSM medium after 4 d of culture, while the strain lost its degradation ability when the beta-cyp concentration reached 250 mg/L. The effects of LP 3–27 on fermentation, bacterial community, and bioremediation of contaminated alfalfa silage at two dry matter (DM) contents were studied. The results showed that inoculation with LP3–27 not only degraded beta-cyp, but also improved the fermentation quality of alfalfa silage after 60 d of ensiling. Meanwhile, L. pentosus dominated the bacterial community during ensiling in LP3–27 inoculated silages, whereas Pediococcus acidilactici was the dominant species in the control silage. LP3–27 inoculation also simplified the bacterial interaction networks of ensiled alfalfa. Beta-cyp degradation was positively correlated with L. pentosus in LP- inoculated silages, which confirmed the function of beta-cyp degradation by L. pentosus . In addition, higher beta-cyp degradation was observed in silage with 35% versus 43% DM. In summary, strain LP3–27 could be used as a candidate inoculum for bioremediation of beta-cyp-contaminated silage and to produce safe silage for animal production.
研究领域:
微生物学
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环境科学
·
动物营养学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yaqing Yin,Shanjia Qin,Shuai Deng,Zhili Li,Aixing Tang,Qunliang Li,Dankui Liao,Youyan Liu
Lignin-based nanoparticles hold tremendous potential for various applications. This study proposes an innovative and straightforward method for the synthesis of spherical hybrid lignin nanoparticles (hy-LNPs) with a tunable pore structure. The approach involves blending lignin with 20 wt% polyamide-epichlorohydrin, resulting in the formation of thermoresponsive lignin-based polyelectrolyte complexes. Upon heating to 80 °C, the complexes undergo self-assembly into uniform spherical nanoparticles, achieving a minimum polydispersity index (PDI) as low as 0.08. The study reveals that nanoparticle formation involves simultaneous collapse and growth. During collapse, hy-LNPs become more compact, increasing their elastic behavior and inhibiting particle coalescence, which is critical for the formation of stable, low-dispersibility nanoparticles. Contrary to the expectation that collapse would reduce pore size, the average pore size of the hy-LNPs increases from 24.9 nm to 35.8 nm, likely due to the coalescence of smaller pores into larger ones. Furthermore, this straightforward method was applied to encapsulation β-cypermethrin, achieving an encapsulation efficiency of up to 95 % and reducing the release rate in an ethanol-water solution from 90.6 % to 63.1 % over 5 h. The thermoresponsive lignin-based polyelectrolyte complexes provide a new pathway for the controlled preparation of lignin-based nanoparticles. These nanoparticles demonstrate promising potential for applications such as drug encapsulation.
研究领域:
材料科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2013
·Vol. 129
·期 4
·1952-1958
·
IF 1.395
Wei-Sheng Guan,Jun-Ru Lei,Xu Wang,Ya Zhou,Chen-Chen Lu,Shao-Fang Sun
Magnetic nanoparticles were attached to yeast by co-precipitation reaction of FeCl 3 ·6H 2 O and FeCl 2 ·4H 2 O. Then, based on magnetite yeast composites (M@Y), the magnetic molecularly imprinted polymers (MMIPs) were synthesized for the selective recognition of beta-cypermethrin (PP 321 ). MMIPs were characterized by scanning electron microscopy, X-ray diffraction, vibrating sample magnetometer, Fourier transform infrared analysis, thermogravimetric analysis, and elemental analysis. MMIPs exhibited uniform morphology and magnetic property (Ms = 17.87 emu/g) and thermal stability. Batch mode adsorption studies were carried out to investigate the specific adsorption equilibrium, kinetics, and selective recognition. The Langmuir isotherm model was fitted to the equilibrium data slightly better than the Freundlich model, and the adsorption capacity of MMIPs was 39.64 mg/g at 298 K. The kinetic properties of MMIPs were well described by the pseudo-second-order equation. Hydrogen bonds between methacrylic acid and PP 321 were mainly responsible for the adsorption mechanism. The MMIPs prepared were applied to the separation of PP 321 from experimental samples successfully. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
LIPIDS
·
2020
·Vol. 55
·期 3
·251-260
·
IF 1.919
Bingnan He,Xia Wang,Xini Jin,Zimeng Xue,Yinhua Ni,Jianbo Zhu,Caiyun Wang,Yuanxiang Jin,Zhengwei Fu
Studies have elucidated that pyrethroids induce adipogenesis. It is also known that macrophages can affect the homeostasis of adipose tissue. However, whether and how the β-cypermethrin (β-CYP)-mediated inhibition of the macrophages affects adipogenesis remain unknown. To explore the effects of β-CYP on adipogenesis through modulating the function of macrophages, 3T3-L1 cells, a preadipocyte cell line, were exposed to culture medium from either RAW 264.7 cells, a macrophage cell line (RM), or β-CYP-treated RAW 264.7 cells (CRM). CRM decreased the inhibitory effects of RM treatment on cell proliferation and adipogenesis, as lipid accumulation, the CEBPA content, and Fasn and Acaca expression in 3T3-L1 cells were higher following CRM treatment than following RM treatment through the higher levels of the demethylated CEBPA promoter in 3T3-L1 cells. However, the medium from β-CYP- and N-acetyl-L-cysteine-cotreated RAW 264.7 cells (CNRM) partially restored the inhibitory effects of RAW 264.7 cells on 3T3-L1 cells that had been reduced by CRM, indicating that β-CYP might reduce the cytotoxicity and inhibitory effects of RAW 264.7 cells on the adipogenesis of 3T3-L1 cells through elevating ROS levels in RAW 264.7 cells. Moreover, exposure to β-CYP downregulated the TNF-α secretion in RAW 264.7 cells. In conclusion, these data demonstrated that β-CYP affected the function of RAW 264.7 cells, alleviating their inhibitory effects on adipogenesis and CEBPA demethylation in 3T3-L1 cells. β-CYP might achieve these effects through downregulating the secretion of TNF-α via elevating ROS levels in RAW 264.7 cells. Our experiments provide a new perspective on the obesogenic effect of pyrethroids.
研究领域:
毒理学
·
细胞生物学
·
分子生物学
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Yingjian Ma,Rui Zhao,Hongyi Shang,Shuo Zhen,Linjie Li,Xinyu Guo,Meng Yu,Yong Xu,Jianguo Feng,Xuemin Wu
Pesticides are the primary defending strategy against major biological invaders and important for ensuring national food security. Intelligent nanopesticides with precise controlled release modes are currently an emerging area of interest in the field of green, safe, and sustainable pesticide formulation. In this study, β-cypermethrin (β-CYP) was loaded into ZIF-8 by the one-pot method, which successfully prepared a pH-responsive delivery nanoparticle (β-CYP/ZIF-8) to control termites. Results demonstrated that β-CYP/ZIF-8 had a mean diameter of 188 ± 2 nm and a loading capacity of approximately 21%. In vitro release experiments showed that β-CYP/ZIF-8 releases better in acidic than in neutral conditions due to the presence of intermediate acid-unstable imidazole organic compounds. The acidic microenvironment in which termites thrive can promote β-CYP release due to ZIF-8 decomposition, thus improving pesticide targeting and reducing toxic side effects. The bioactivity survey confirmed that β-CYP/ZIF-8 could control termites (Coptotermes formosanus Shiraki) for a longer duration compared to commercial wettable powder (WP) and emulsifiable concentrate (EC). Moreover, an acute toxicity test showed an over 2- and 10-fold reduction in the acute toxicity of β-CYP/ZIF-8 to earthworms and zebrafish, respectively, compared to that of commercial WP and EC. Finally, the cytotoxicity assay of β-CYP/ZIF-8 showed higher safety to human hepatocyte LO2 cells. Overall, these β-CYP/ZIF-8 nanoparticles, prepared using the one-pot method, have great application potential in sustainable pest management and protection of the ecological environment.
研究领域:
农业科学
·
环境科学
·
纳米技术
·
农药化学
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产品关联
DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2020
·Vol. 77
·期 4
·2129-2141
·
IF 3.75
Li-Sha Bai,Jing-Jing Xu,Cai-Xia Zhao,Ya-Li Chang,Yan-Ling Dong,Kai-Ge Zhang,Yong-Qiang Li,Yi-Ping Li,Zhi-Qing Ma,Xi-Li Liu
BACKGROUND Carboxylesterase (CarE) is a major class of enzyme involved in the detoxification of toxic xenobiotics in various insect species. Previous work has shown that the carboxylesterase gene CarE001G found in Helicoverpa armigera is more active and can metabolize synthesized pyrethroids, such as β -cypermethrin, one of the commonly used commercial insecticides for lepidopteran pest control. In addition, CarE001G is very special as it has a very specific glycine-rich region located adjacent to its C-terminal. But whether mutations in this unique sequence can change the biochemistry and function of CarE001G are unknown. RESULTS In this study, four variants of CarE001G with different deletions in the glycine-rich region were obtained and functionally expressed in Escherichia coli . The recombinant proteins were purified and confirmed by Western blot and mass spectrometry analyses. These mutant enzymes showed high catalytic efficiency toward the model substrate α -naphthyl acetate. Inhibition study showed that β -cypermethrin had relatively strong inhibition on CarE activities. In vitro metabolism assay showed that the mutant enzymes significantly enhanced their metabolic activities toward β -cypermethrin with specific activities between 4.0 and 5.6 nmol L -1 min −1 mg −1 protein. Molecular docking analyses consistently demonstrated that deletion mutations in the glycine-rich region may facilitate the anchoring of the β -cypermethrin molecule in the active binding pocket of the mutant enzymes. CONCLUSION The data show that deletion mutations can cause qualitative change in the capacity of CarEs in the detoxification of β -cypermethrin. This indicates that deletion mutations in the glycine-rich region may have the potential to cause synthesized pyrethroid (SP) resistance in H. armigera in the future. © 2020 Society of Chemical Industry
研究领域:
昆虫学
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分子生物学
·
农药毒理学
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产品关联
DOI
PubMed
高影响因子
POULTRY SCIENCE
·
2024
·
IF 3.8
Xuedi Zhang,Yue Zhang,Kai Xu,Jianhua Qin,Dehe Wang,Lijun Xu,Chuanwen Wang
ABSTRACT Dermanyssus gallinae is a major hematophagous ectoparasite in layer hens. Although the acaricide β-cypermethrin has been used to control mites worldwide, D. gallinae has developed resistance to this compound. Carboxylesterases ( CarEs ) are important detoxification enzymes that confer resistance to β-cypermethrin in arthropods. However, CarEs associated with β-cypermethrin resistance in D. gallinae have not yet been functionally characterized. Here, we isolated a CarE gene ( Deg-CarE ) from D. gallinae and assayed its activity. The results revealed significantly higher expression of Deg-CarE in the β-cypermethrin-resistant strain ( RS ) than in the susceptible strain ( SS ) toward α-naphthyl acetate ( α-NA ) and β-naphthyl acetate ( β-NA ). These findings suggest that enhanced esterase activities might have contributed to β-cypermethrin resistance in D. gallinae . Quantitative real-time PCR analysis revealed that Deg-CarE expression levels were significantly higher in adults than in other life stages. Although Deg-CarE was upregulated in the RS, significant differences in gene copy numbers were not observed. Additionally, Deg-CarE expression was significantly induced by β-cypermethrin in both the SS and RS. Moreover, silencing Deg-CarE via RNA interference decreased the enzyme activity and increased the susceptibility of the RS to β-cypermethrin, confirming that Deg-CarE is crucial for β-cypermethrin detoxification. Finally, recombinant Deg-CarE ( rDeg-CarE ) expressed in Escherichia coli displayed high enzymatic activity toward α/β-NA. However, metabolic analysis indicated that rDeg-CarE did not directly metabolize β-cypermethrin. The collective findings indicate that D. gallinae resistance to β-cypermethrin is associated with elevated CarEs protein activity and increased Deg-CarE expression levels. These findings provide insights into the metabolic resistance of D. gallinae and offer scientific guidance for the management and control of D. gallinae .
研究领域:
昆虫学
·
分子生物学
·
害虫管理
·
生物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）