INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Shujie Ma,Ran Jia,Menglei Guo,Kaitao Qin,Lihui Zhang
Plant essential oils (EOs) could be used as new alternatives of synthetic insecticides due to their environmental friendliness, safety to non-target organisms, and low-level resistance. In this study, the essential oil of Cephalotaxus sinensis (CSEO), an important Chinese medicinal species, showed potent insecticidal activity against Megoura japonica, Plutella xylostella and Sitophilus zeamais in laboratory bioassays; CSEO 20 % emulsifiable concentrate exhibited the control efficacy of 85.67 % against M . japonica after 7 days treatment at concentration of 0.25 % (w/v) in the greenhouse experiment. The GC-MS results showed that the main components of CSEO were α-pinene (37.89 %), β-caryophyllene (16.78 %) and germacrene D (10.79 %). Five active compounds, β-elemene (1) , β-caryophyllene (2) , β-phellandrene (3) caryophyllene oxide (4) and α-pinene (5) were isolated from CSEO. β-Caryophyllene (2) exhibited highest contact toxicity against M. japonica and P. xylostella , with the median lethal dose (LD 50 ) values of 0.072 μg/adult and 0.32 μg/larva, respectively. α-Pinene (5) showed the highest fumigant toxicity against M. japonica and P. xylostella , with the median lethal concentration (LC 50 ) values of 6.74 and 7.35 mg/L, respectively. Furthermore, β-phellandrene (3) had the potent contact and fumigant toxicity against S. zeamais with the LD 50 and LC 50 value of 2.19 μg/adult and 3.57 mg/L, respectively. Thus, CSEO and their major components have the potential to be developed as novel botanical insecticides for the control of agricultural pests.
研究领域:
植物学
·
农业科学
·
害虫防治
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DOI
高影响因子
Environmental Science & Technology Letters
·
2023
·
IF 10.9
Lin Du,Li Xu,Kun Li,Christian George,Maofa Ge
Anthropogenic air pollutants can be involved in biogenic secondary organic aerosol (SOA) formation. However, such interactions are currently one of the least understood aspects of atmospheric chemistry. Herein, SOA formation via chemical interactions between anthropogenic SO2, NH3, and O3 and biogenic β-caryophyllene was investigated. It is shown that although SO2 considerably enhanced SOA formation, this enhancing effect was weakened by NH3 when SO2 and NH3 coexisted. NH3-induced neutralization of particle acidity generated by SO2 oxidation may be the primary driving factor of this weakening effect. Molecular-level characterization using high-resolution quadrupole time-of-flight mass spectrometry revealed additional connections between NH3-induced changes in SOA composition and aerosol acidity. Specifically, the lower relative abundances of several main products generated in the presence of SO2 and NH3 than those formed in the presence of only SO2 were consistent with their suppressed formation by lower seed acidity. The suppression of oligomer formation by NH3 provided more evidence for the weakening of acid-catalyzed processes caused by acidity neutralization. Accordingly, the current study demonstrates that NH3 as a less effectively regulated alkaline gas resulting from an unbalanced reduction of different pollutants must be considered with caution when evaluating effects of SO2 on SOA formation via anthropogenic–biogenic interactions.
研究领域:
大气化学
·
环境科学
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DOI
高影响因子
FITOTERAPIA
·
2025
·
IF 2.6
Li Zhang,Hui Wu,Xiaoyuan Li,Xiu Zhang,Hua Li,Huaqiao Tang,Daxuan Tang,Shijing Du,Ya’’ou Liu,Yangfan Tang,Xiaoming Bao,Guoqiang Cheng
Qianghuo (the rhizomes and radixes of Notopterygium incisum Ting ex H.T. chang) is a traditional Chinese medicine, and widely used as an anti-inflammatory, anodyne, antifebrile agent. Qianghuo volatile oil also exhibits the same properties, and the effects are probably related to the inhibition of cyclooxygenase (COX), but its bioactive components are not clear yet. To figure out the bioactive components of Qianghuo volatile oil target to cyclooxygenase-2 (COX-2), a ligand fishing method using immobilized COX-2 magnetic beads (COX-2-MB S ) and gas chromatography–mass spectrometry (GC–MS) were developed to identify the compounds that act on COX-2. Nine ligands, including 3-carene, β-caryophyllene, eucalyptol, α-terpinene, Limonene, δ-terpinene, α-pinene, β-pinene and γ-terpinene, were specifically captured by COX-2-MB S . Among them, 3-carene, β-caryophyllene, and eucalyptol in Qianghuo volatile oil were isolated and confirmed by in vitro inhibition and in vivo anti-inflammatory activity assays as selective inhibitor against COX-2. The IC 50 values were 13.5, 10.1, and 7.6 μM, respectively. Compared with the model, 3-carene, β-caryophyllene, and eucalyptol considerably reduced ear swelling and paw edema. Moreover, 3-carene and eucalyptol significantly decreased the changes of COX-2 expression in RAW264.7 cells activated by LPS. Our study validated that the developed ligand-fishing method using COX-2-MBs coupled with GC–MS can be applied to recognize COX-2 inhibitors in Qianghuo volatile oil. In vitro/vivo assay showed 3-carene and eucalyptol in Qianghuo volatile oil had strong inhibition against COX-2. This study may provide novel methods for discovering active components for volatile oil–based drug development or aromatherapy.
研究领域:
中药学
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生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ECONOMIC ENTOMOLOGY
·
2015
·Vol. 108
·期 3
·957-961
·
IF 1.506
Tian Bao-liang,Liu Qi-Zhi,Liu Zhi-Long,Li Peng,Wang Jie-Wen
Cacopsylla chinensis (Yang and Li) (Hemiptera: Psyllidae) is an important pest of pear in China. As an alternative to conventional chemical pesticides, botanicals including essential oils and their constituents could provide an eco-friendly and nonhazardous control method. In this study, the essential oil of clove buds ( Syzygium aromaticum ) was obtained by hydrodistillation. Five constituents, accounting for 99.89% of the oil, were identified by gas chromatography-mass spectrometry, and the major constituents were eugenol (88.61%) and eugenol acetate (8.89%), followed by β -caryophyllene (1.89%). In a laboratory bioassay, clove essential oil, commercial eugenol (99.00%) and β -caryophyllene (98.00%) exhibited strong contact toxicity against the summerform adults of C. chinensis with LD 50 values of 0.730, 0.673, and 0.708 µg/adult, and against the nymphs with LD 50 values of 1.795, 1.668, and 1.770 µg/nymph, respectively. In contrast, commercial eugenol acetate (98%) had LD 50 values of 9.266 µg/adult and 9.942 µg/nymph. In a field trial, clove essential oil caused significant population reductions of 73.01% (4.80 mg/ml), 66.18% (2.40 mg/ml) and 46.56% (1.20 mg/ml), respectively. Our results demonstrated that clove essential oil and its constituents have potential as a source of natural insecticides.
研究领域:
植物保护
·
昆虫学
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产品关联
DOI
PubMed
高影响因子
ACS Synthetic Biology
·
2023
·
IF 4.7
Surui Lu,Hong Deng,Chenyao Zhou,Zhengda Du,Xuena Guo,Yanfei Cheng,Xiuping He
β-Caryophyllene is a plant-derived bicyclic sesquiterpene with multiple biological functions. β-Caryophyllene production by engineered Saccharomyces cerevisiae represents a promising technological route. However, the low catalytic activity of β-caryophyllene synthase (CPS) is one of the main restrictive factors for β-caryophyllene production. Here, directed evolution of the Artemisia annua CPS was performed, and variants of CPS enhancing the β-caryophyllene biosynthesis in S. cerevisiae were obtained, in which an E353D mutant enzyme presented large improvements in Vmax and Kcat. The Kcat/Km of the E353D mutant was 35.5% higher than that of wild-type CPS. Moreover, the E353D variant exhibited higher catalytic activity in much wider pH and temperature ranges. Thus, both the higher catalytic activity and the robustness of the E353D variant contribute to the 73.3% increase in β-caryophyllene production. Furthermore, the S. cerevisiae chassis was engineered by overexpressing genes related to β-alanine metabolism and MVA pathway to enhance the synthesis of the precursor, and ATP-binding cassette transporter gene variant STE6T1025N to improve the transmembrane transport of β-caryophyllene. The combined engineering of CPS and chassis resulted in 70.45 mg/L of β-caryophyllene after 48 h of cultivation in a test tube, which was 2.93-fold of that of the original strain. Finally, a β-caryophyllene yield of 594.05 mg/L was obtained by fed-batch fermentation, indicating the potential of β-caryophyllene production by yeast.
研究领域:
生物技术
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代谢工程
·
合成生物学
·
酶工程
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2020
·Vol. 76
·期 7
·2453-2464
·
IF 3.75
Zhengbing Wang,Chuanhe Gao,Jiali Liu,Wenwu Zhou,Xinnian Zeng
BACKGROUND The Asian citrus psyllid (ACP), Diaphorina citri , is one of the major pests in citrus production because it transmits huanglongbing, a devastating disease of citrus plants. Odourant-binding proteins (OBPs) play an important role in the olfactory perception of insects. Revealing the function of DcitOBPs is beneficial to the development of new ACP management strategies. RESULTS An analysis of the components of volatiles from the new shoots of six host plant species showed that β-caryophyllene was the characteristic volatile compound in flushing shoots and the most abundant volatile compound in three of the six tested ACP host plant species. The tissue expression profiles of nine known DcitOBPs were analyzed based on a transcriptome database, and DcitOBP3 and DcitOBP6 exhibited high expression in the antennae of both sexes and the ovipositor of females. The binding ability of two recombinant proteins with eight ligands was studied through competitive binding analysis; the results showed that DcitOBP6 exhibited stronger binding to β-caryophyllene. Behavioural trials indicated that sexually mature female adults of D. citri were significantly attracted to β-caryophyllene at concentrations of 0.1 μL mL −1 and 10 μL mL −1 . RNAi analysis in female D. citri showed that the reduction of DcitOBP6 transcript abundance led to a decrease in antennae EAG activity and behavioural responses to β-caryophyllene. CONCLUSION The results demonstrate that DcitOBP6 is involved in the perception of an important host plant volatile, β-caryophyllene, in the ACP, and provide a theoretical foundation for behavioural interference in ACP management.
研究领域:
昆虫学
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分子生物学
·
害虫管理
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 24
·16055
·
IF 6.208
Yan-Ting Wu,Li-Shan Zhong,Chen Huang,Yu-Ying Guo,Fu-Jun Jin,Yu-Ze Hu,Zi-Bo Zhao,Zhe Ren,Yi-Fei Wang
Macrophage infiltration is one of the main pathological features of ulcerative colitis (UC) and ferroptosis is a type of nonapoptotic cell death, connecting oxidative stress and inflammation. However, whether ferroptosis occurs in the colon macrophages of UC mice and whether targeting macrophage ferroptosis is an effective approach for UC treatment remain unclear. The present study revealed that macrophage lipid peroxidation was observed in the colon of UC mice. Subsequently, we screened several main components of essential oil from Artemisia argyi and found that β-caryophyllene (BCP) had a good inhibitory effect on macrophage lipid peroxidation. Additionally, ferroptotic macrophages were found to increase the mRNA expression of tumor necrosis factor alpha (Tnf-α) and prostaglandin-endoperoxide synthase 2 (Ptgs2), while BCP can reverse the effects of inflammation activated by ferroptosis. Further molecular mechanism studies revealed that BCP activated the type 2 cannabinoid receptor (CB2R) to inhibit macrophage ferroptosis and its induced inflammatory response both in vivo and in vitro. Taken together, BCP potentially ameliorated experimental colitis inflammation by inhibiting macrophage ferroptosis. These results revealed that macrophage ferroptosis is a potential therapeutic target for UC and identified a novel mechanism of BCP in ameliorating experimental colitis.Keywords:ulcerative colitis;ferroptosis;β-caryophyllene;type 2 cannabinoid receptor;inflammation;macrophage
研究领域:
免疫学
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药理学
·
胃肠病学
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产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2022
·
IF 5.307
Chunmei Bai,Qianqian Ma,Qi Li,Lijun Yu,Dong Zhen,Mingjie Liu,Chengxi Wei
Combination drug therapy has become an effective strategy for chronic metabolic disease, especially cardiovascular disease. In the present study, possible drug combinations were screened and the mechanism of the combinations against cardiac hypertrophy was examined within 1,8-cineole, β -caryophyllene, linalool, and β -pinene.H9c2 cells were treatment with 1,8-cineole, β -caryophyllene, linalool, and β -pinene individually or in combination for 24 h after isoprenaline stimulation. Cell viability was detected by the MTT assay. Subsequently, bioinformatic analysis and network pharmacology were used to reveal the multi-targeted synergistic therapeutic effect of the combination treatment compounds on cardiac hypertrophy. Ultimately, western blot and elisa was performed to analyses the protein expression in vivo . MTT results found that 1,8-cineole and β -caryophyllene synergistically increased cell viability with CalcuSyn software analyses. Specifically, bioinformatic and network pharmacology analysis showed PTGS2, TNF, IL-6, AKT1, NOS2, and CAT were identified as the key targets. P13K-AKT signaling pathway was involved in the reversal of cardiac hypertrophy by the combination of 1,8-cineole and β -caryophyllene. The in vitro results indicated that the combination synergistically treated the isoprenaline-induced mice against structural and functional myocardial damage via the P13K-AKT signaling pathway. Collectively, the combined application of 1,8-cineole and β -caryophyllene synergistically reverses cardiac hypertrophy in isoprenaline-induced H9c2 cells and mice.
研究领域:
药理学
·
心血管疾病
·
细胞生物学
·
网络药理学
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产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
·
2023
·
IF 3.4
Nan Zhang,Junwei Zhang,Weiqi Cui,Deqiao Wu,Jingxian Zhang,Bo Wei,Lingbo Qu,Xia Xu
Artemisia argyi H.Lév. & Vaniot, a traditional Chinese medicine with a history spanning over two millennia, has been extensively used in folk medicine to treat dysmenorrhea, uterine bleeding and inflammation. Recent studies have demonstrated that the essential oil extracted from Artemisia argyi H.Lév. & Vaniot, known as AAEO, exhibits significant anti-tumor properties against liver and lung cancers. There is a scarcity of research on the potential impact of AAEO on pancreatic cancer (PC) cells. In this study, UPLC-MS/MS-based metabolomics method was established to evaluate the effect of AAEO on the proliferation of PC cells. The differential compounds included 5-oxoproline, glutamate, γ-glutamylcysteine, glutathione, arachidonic acid, adrenal acid and linoleic acid were detected by metabolomics, enriching in the γ-glutamyl cycle and polyunsaturated fatty acid metabolism, which were closely related to ferroptosis. Meanwhile, AAEO dramatically increased the levels of intracellular iron ion via up-regulation of TFR1, augmented reactive oxygen species and malondialdehyde in a dose-dependent manner by down-regulation of γ-glutamyl cycle through decreasing expressions of SLC7A11. Additionally, β-caryophyllene oxide, one of the main components of AAEO, could covalently bind to Cys in SW1990 cells to form a conjugate Cpo-Cys, resulting in the inhibition of glutathione synthesis. Importantly, the ferroptosis inhibitor deferoxamine significantly blocked the inhibitory effect of AAEO on SW1990 cells. Meanwhile, β-caryophyllene oxide, dihydro-β-ionone and α-bisabolol had strong binding force with GPX4, SLC7A11 and TFR1, respectively. These findings showed that AAEO induced ferroptosis via regulation of γ-glutamyl cycle by SLC7A11 and iron disorders by TFR1. Our study discovered AAEO as a potential therapeutic approach to induce ferroptosis to prevent or treat PC.
研究领域:
肿瘤学
·
药理学
·
代谢组学
·
中医药
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产品关联
DOI
PubMed
高影响因子
Journal of Analytical Methods in Chemistry
·
2021
·
IF 2.193
Guanjun Nan,Lina Zhang,Zhengzheng Liu,Yu Liu,Yan Du,Hongwen Zhao,Hongxia Zheng,Rong Lin,Guangde Yang,Shaohua Zheng
Eupatorium fortunei Turcz. is a widely used Chinese herbal medicine in China. In this study, a gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) method was developed and validated to simultaneously determine the contents of p-cymene, thymol, neryl acetate, and β-caryophyllene in roots, stems, and leaves of Eupatorium fortunei Turcz. harvested at different growth periods. All four constituents could be detected in leaves, three could be detected in stems except β-caryophyllene, and only thymol could be detected in roots. The order of the total contents of four constituents in different parts was leaves > stems > roots. It indicated that the leaves could be the proper medicinal parts of Eupatorium fortunei Turcz. The content of four constituents in leaves varied a lot among different growth periods and showed an M-shaped change trend with the growth of Eupatorium fortunei Turcz. The four constituents accumulated to the highest values in early July followed by mid-September. Accordingly, the best harvest time of Eupatorium fortunei Turcz. is early July and mid-September.
研究领域:
药用植物学
·
分析化学
·
植物科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2022
·
IF 2.267
Zuojian Zhang,Haijie Wang,Bo Yang,Jinian Shu,Zhangqi Yu,Zhiyang Wei,Jingyun Huang,Kui Jiang,Yedong Guo,Zhen Li
Recently, photoinduced associative ionization (PAI) has been demonstrated to be extremely efficient for the determination of oxygenated volatile organic compounds. However, its application for the determination hydrocarbons has not yet been reported. In this study, associative ionization of nine typical monoterpenes induced by dichloromethane (CH2Cl2) under vacuum ultraviolet (VUV) light was investigated using a laboratory-constructed PAI time-of-flight mass spectrometer (PAI-TOFMS). Without doping CH2Cl2, VUV light generated ordinary photoionization mass spectra of monoterpenes that were dominated by fragment ions, while the molecular ions were inconspicuous. In contrast, doping with CH2Cl2 resulted in the appearance of mass peaks of the characteristic protonated molecular ions of the monoterpenes. The PAI method significantly enhanced the signals for the monoterpenes. The intensities of protonated molecular ions derived from PAI were amplified by 52 to 108 fold compared with those of molecular ions produced by VUV photoionization (VUV PI). With the high ionization efficiency of PAI toward monoterpenes, the sensitivities of PAI-TOFMS toward limonene, β-ocimene, β-myrcene, β-pinene, and α-pinene were from 291 ± 6 to 1338 ± 20 counts pptv−1 using an acquisition time of 30 s. The limits of detection were from 0.32 to 1.45 pptv, equivalent to between 1.92 and 8.83 ng m−3. The applicability of this ionization mode was further characterized by the determination of monoterpenes from potted Sabina chinensis. This study provides a new and highly efficient ionization method for the determination of plant monoterpenes using mass spectrometry.
研究领域:
分析化学
·
环境科学
·
质谱学
·
植物科学
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产品关联
DOI
高影响因子
Microbial Cell Factories
·
2016
·Vol. 15
·期 1
·1-9
·
IF 3.744
Yang Jianming,Nie Qingjuan
Background Under aerobic conditions, acetic acid is the major byproduct produced by E. coli during the fermentation. And acetic acid is detrimental to cell growth as it destroys transmembrane pH gradients. Hence, how to reduce the production of acetic acid and how to utilize it as a feedstock are of intriguing interest. In this study, we provided an evidence to produce β-caryophyllene by the engineered E. coli using acetic acid as the only carbon source. Results Firstly, to construct the robust acetate-utilizing strain, acetyl-CoA synthases from three different sources were introduced and screened in the E. coli . Secondly, to establish the engineered strains converting acetic acid to β-caryophyllene, acetyl-CoA synthase (ACS), β-caryophyllene synthase (QHS1) and geranyl diphosphate synthase (GPPS2) were co-expressed in the E. coli cells. Thirdly, to further enhance β-caryophyllene production from acetic acid, the heterologous MVA pathway was introduced into the cells. What’s more, acetoacetyl-CoA synthase (AACS) was also expressed in the cells to increase the precursor acetoacetyl-CoA and accordingly resulted in the increase of β-caryophyllene. The final genetically modified strain, YJM67, could accumulate the production of biomass and β-caryophyllene up to 12.6 and 1.05 g/L during 72 h, respectively, with a specific productivity of 1.15 mg h −1 g −1 dry cells, and the conversion efficiency of acetic acid to β-caryophyllene (gram to gram) reached 2.1 %. The yield of β-caryophyllene on acetic acid of this strain also reached approximately 5.6 % of the theoretical yield. Conclusions In the present study, a novel biosynthetic pathway for β-caryophyllene has been investigated by means of conversion of acetic acid to β-caryophyllene using an engineered Escherichia coli . This was the first successful attempt in β-caryophyllene production by E. coli using acetic acid as the only carbon source. Therefore, we have provided a new metabolic engineering tool for β-caryophyllene synthesis.
研究领域:
生物技术
·
代谢工程
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）