INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yiming Jiang,Shiqi Zhang,Linfan Pan,Juncai Leng,Tingyi Zhou,Mingxuan Liu,Li Li,Wei Zhao
Fat and its hydrolysis products, fatty acids, are indispensable nutritional components; however, prolonged excessive fat consumption, particularly in western diets, contributes to the onset of obesity and multiple metabolic disorders . In this study, we propose a daily-ingestible hydrogel (denoted as β C-MA hydrogel) composed of natural β -glucan and sodium carboxymethylcellulose crosslinked by malic acid at 120 °C. This hydrogel exhibits rapid swelling performance, up to 24-fold within 1 min and 176-fold after 1 h in deionized water . It also lengthens gastric retention and increases endogenous satiety signal levels, potentially controlling appetite and reducing food intake. Furthermore, β C-MA hydrogels that enter the small intestine can effectively inhibit fat hydrolysis and decrease triglyceride synthesis and transport. Specifically, the hydrogels inhibit the release of free fatty acids (FFAs) by approximately 50 % during digestion, influence the translocation of triglycerides and FFAs across the intestinal epithelium, and reduce the serum triglyceride levels by 22.2 %. These findings suggest that β C-MA hydrogels could serve as a noninvasive gastrointestinal device for weight control, with the advantage of reducing food intake and restoring lipid metabolism homeostasis.
研究领域:
营养学
·
生物医学工程
·
代谢研究
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Zhen Wu,Chenyang Zhao,Yongxia Huang,Fayin Ye,Guohua Zhao
Environmental temperature and pH induced significant changes in the size and surface charge ( ζ ) of octenylsuccinated oat β -glucan aggregates. The underlying mechanisms were explored by using 1 H-NMR, fluorescence spectra, thermodynamic analysis, and SAXS. At pH 6.5, the size decreased with temperature while ζ continuously increased. With increasing pH at 293 K, parabolic and U-shaped trends were observed in the size and ζ , peaking at pH 8.5 and 6.5, respectively. At any tested pH, the size decreased with temperature. Overall, ζ significantly increased with temperature at each pH. As temperature increased, the compactness of hydrophobic-domains increased while the compactness of hydrophilic-domains decreased. In an acidic environment, both the compactness increased with decreasing pH, but in an alkaline environment, they decreased with pH. The compactness changes were co-driven by enthalpy and entropy and corresponded to changes in the hydrophobic interactions in hydrophobic-domains, hydrogen bonds in hydrophilic-domains and electrostatic repulsions among octenylsuccinate molecules.
研究领域:
食品科学
·
分子生物学
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DOI
高影响因子
BIOMACROMOLECULES
·
2019
·
IF 5.667
Bingchao Duan,Siwei Zou,Ying Sun,Xiaojuan Xu
A nanoplatform carrying doxorubicin (Dox) for cancer therapy and a dye for imaging was developed based on a natural triple helix β-glucan (t-LNT) and polydeoxyadenylic acid (poly(dA)). The t-LNT-Dox conjugates were prepared through Schiff-base reaction between the aldehyde group in the oxidized t-LNT and the amino group of Dox, the single chains (s-LNT-Dox) of which interacted with the poly(dA)-dye to form a composite s-LNT-Dox/poly(dA)-dye through hydrogen bonding between s-LNT and poly(dA). t-LNT-Dox was confirmed to acid-responsively release Dox in vitro, showing enhanced cytotoxicity against HeLa cancer cells with time. It was confirmed that Dox and the dye could be simultaneously delivered into HeLa cells or the tumors with a prolonged duration time. Furthermore, LNT-Dox conjugates effectively inhibited tumor growth and decreased adverse effects of the free Dox in vivo. Hence, this work develops a new strategy to fabricate the nanoplatform for therapy and imaging using a natural polysaccharide.
研究领域:
生物材料学
·
肿瘤学
·
药剂学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yonggan Sun,Shanshan Zhang,Huijun He,Haihong Chen,Qixing Nie,Song Li,Jiaobo Cheng,Baojie Zhang,Zhitian Zheng,Shijie Pan,Ping Huang,Lu Lian,Jielun Hu,Shaoping Nie
Dietary fiber is crucial for human health mainly due to its impact on gut microbiota structure and metabolites. This study aimed to investigate the impact of Dendrobium officinale polysaccharides (DOP) and two common fibers (β-glucan and inulin) on the gut microbiome structure and metabolic profile in vitro . Fecal samples were obtained from 30 healthy volunteers, which were then individually subjected to fermentation with each type of fiber. The results revealed that all fibers were efficiently degraded by gut microbiota, with DOP exhibiting a slower fermentation rate compared to β-glucan and inulin . The fermentation of all fibers led to a significant increase in the production of short-chain fatty acids (SCFAs) and a reduction in branched-chain fatty acids (BCFAs), sulfides, phenols, and indole. Moreover, the abundance of unclassified Enterobacteriaceae , which was positively correlated with sulfide, phenols, and indole levels, was significantly reduced by all fibers. Additionally, DOP specifically promoted the growth of Parabacteroides , while β-glucan and inulin promoted the growth of Bifidobacterium and Faecalibacterium . Taken together, these findings enhance our understanding of the role of DOP, β-glucan, and inulin in modulating gut microbiota and metabolites, where the fermentation with fecal bacteria from different volunteers could provide valuable insights for personalized therapeutic approaches.
研究领域:
食品科学
·
微生物学
·
营养学
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DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Yu-Xiao Wang,Lin-Yan Li,Ting Zhang,Jun-Qiao Wang,Xiao-Jun Huang,Jie-Lun Hu,Jun-Yi Yin,Shao-Ping Nie
Glucans of multiple architecture and bioactivities are widely distributed in cereals. In our previous study, the barley water-soluble fiber (BSF) has been proved to possess hypoglycaemic and hypolipidaemic effects. A further attempt in the present study is to comprehensively demonstrate the structures and solution properties of main constituents in BSF. Two mainly homogeneous fractions (BSF-20 and BSF-80r) were purified from BSF, and their physicochemical and structural characterization were investigated. Methylation and 1D/2D NMR experiments indicated that BSF-20 was a linear β-glucan, composed of β-D-Glc p residues via (1 → 4)-linkages, and occasionally single (1 → 3)-linkage. The high performance size-exclusion chromatography coupled with multiangle laser light scattering, X-ray diffraction, rheological measurements and scanning electron microscopy were further conducted to analyze its solution property and morphology. BSF-20 adopted a linear flexible chain in aqueous solution, and exhibited high viscosity and strong gelling properties. Intertwined fibrous filaments were visualized by SEM observation. Besides, BSF-80r was a limit dextrin, which was an artefact caused by the incomplete hydrolysis of starch during the isolation of barley soluble fiber. Therefore, the highly viscous β-glucan is the main biomacromolecule occurring in BSF. This work lays the groundwork for the potential application of barley soluble fiber in beverages and other liquid products as new thickeners and stabilizers.
研究领域:
食品科学
·
生物化学
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DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Jinpeng Li,Liang Li
A novel nanoparticle was prepared with soy protein isolate (SPI)-oat β-glucan (OG) conjugates obtained by extrusion (extruded SPI-OG) to stabilize the emulsion. Extruded SPI-OG nanoparticles exhibited a spherical and stable structure. The 90 °C and 110 °C extruded SPI-OG nanoparticles had higher absolute value of zeta potential (13.35 mV and 12.24 mV, respectively) and an average particle size of less than 200 nm. Moreover, interfacial tension of nanoparticles increased with increasing extrusion temperature, but diffusion rate and surface hydrophobicity were decreased, while penetration rate ( K p ) and rearrangement rate ( K r ) increased and then decreased. The 90 °C and 110 °C extruded SPI-OG nanoparticles had the highest K p and K r , and increased by 38.14% and 40.11%, 175.16% and 168.15%, respectively, compared with K p and K r of SPI nanoparticles. And emulsion stabilized by these two nanoparticles exhibited the extraordinary emulsion stability of 81.64% and 80.07%, respectively (p > 0.05). These results demonstrated that adsorption rate of extruded SPI-OG nanoparticles to the oil-water interface decreased, but could rapid unfold and rearrange at the interface to form interfacial layer for stabilizing emulsion. And adsorption capacity and microstructure of emulsion also proved that more extruded SPI-OG nanoparticles could adsorb onto the interface to form a dense interfacial layer. This may provide a theoretical basis for novel nanoparticles as interfacial stabilizer in emulsion.
研究领域:
食品科学
·
生物化学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Jiahua Liu,Zengjun Yang,Khinkhin Phyu,Yu’ang Cao,Han Wang,Xiaoyu Xu,Junfeng Liang,Keqiang Zhang,Chein-Chi Chang,Suli Zhi
Microalgal biofilm can remove nutrients and refractory pollutants from livestock wastewater, transforming them into biomass or fuel. However, these applications are hampered by biofilm shedding. Coculture enhances effects, and its influence on the adhesion of attached microalgal biofilms requires exploration. In this study, two unicellular algae, Euglena (E) and Chlorella pyrenoidosa (C). and two filamentous algae, Klebsodium sp. (K) and Phormidium sp. (P) , were paired two by two, aiming to investigate the effect of mixing on the adhesion of microalgal biofilm. The results showed that suitable microalgal combinations significantly enhanced the performance of the co-cultured microalgal biofilm. Biomass enhancement of 35 % and 21.9 % was observed in the PC group compared to the individual P and C groups, respectively. The content of tightly bound extracellular polymeric substances (TB-EPS) and its protein and polysaccharide content significantly correlating with the net biomass increase in all treatments, p < 0.05 . Furthermore, measurement of zeta potentials and water contact angles indicated that alterations in protein secondary structures contributed to a lower intermolecular repulsion and greater hydrophobicity of the TB-EPS, which together with the stability provided by the higher β-glucan content supported the adhesion of the co-cultured microalgal biofilm.
研究领域:
环境科学
·
微生物学
·
生物技术
·
废水处理
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DOI
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Qian Li,Xuanran Lin,Zhengguang Wang,Dongxu Xing,Fan Liu,Qingyi Wei,Yuxiao Zou
The copigmentation effects and underlying mechanisms of β-cyclodextrin (β-CD), chitosan (CS), guar gum (GG), xanthan gum (XG), and β-glucan (β-GC) on the stability of mulberry anthocyanins (MAS) were systematically investigated. β-CD exhibited the strongest protective effect, followed by CS and β-GC. At 60 °C, β-CD extended the half-life of MAS from 7.42 h to 11.54 h, and significantly reduced the total color difference (ΔE*) value. Molecular docking indicated that β-CD and CS formed stable complexes with cyanidin-3-glucoside (C3G, main monomer in MAS) through the synergistic action of hydrogen bonding, electrostatic interactions, and hydrophobic interaction with high binding free energy. Whereas β-GC, GG, and XG mainly interacted via weak hydrogen bonding. FTIR spectroscopy revealed that β-CD and CS interacted with the C3G hydroxyl and amino vibration groups. Particle size distribution and zeta potential analysis further confirmed that β-CD protected C3G primarily through structural complementarity and inclusion complexation, while CS mainly via electrostatic stabilization.
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2026
·
IF 7.1
Muguo Tang,Yan Zhou,Fa Li,Ruiping Li,Yifan Wang,Siyi Li,Jun Sheng,Liang Tao,Yang Tian
In this study, walnut protein isolate (WNPI) was glycosylated with maltodextrin (M) to prepare a glycosylated protein conjugate (WNPI-M) and its conformational and functional properties were comprehensively analyzed. Subsequently, WNPI-M was employed as a wall material to fabricate walnut oil microcapsules, and the impact of WNPI-M on microcapsule stability was systematically investigated. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and multiple spectroscopic techniques confirmed that glycosylation with M induced significant modifications in WNPI's molecular structure and morphology. The glycosylation profoundly enhanced WNPI's functional properties: solubility at neutral pH increased to 76.94%, emulsifying activity reached 18.16 m 2 /g, and emulsion stability was extended to 244.74 min. Concurrently, the antioxidant capacity of the conjugate was markedly improved. When applied to microencapsulation, WNPI-M demonstrated superior performance in solubility, moisture content, and storage stability of walnut oil microcapsules. These findings substantiate the potential of WNPI-M as a novel, clean-label, and “same-source” wall material for microencapsulation, providing a viable strategy for the high-value utilization of walnut processing by-products.
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产品关联
DOI
高影响因子
Agronomy-Basel
·
2026
·Vol. 16
·期 5
·
IF 4.1
Meng-Ting He,Meng-Yan Li,Jian-Bin Chen,Jiao Du,Xiao-Hua Du,Yue Chen,Xiao-Bin Shi,De-Yong Zhang
Rice (Oryza sativaL.) is a highly valued staple food that is widely consumed worldwide. As the global population continues to grow rapidly, enhancing rice yields becomes essential to meet the increasing demand. β-Glucan has been recognized as a novel immunomodulatory agent. In the present study, we observed that β-glucan stimulates the growth of rice plants and activates the photosynthetic pathway. To explore the underlying molecular mechanisms, we conducted transcriptome sequencing and molecular validation on rice plants treated with either water or β-glucan. Our findings revealed that, compared to plants treated with water, those exposed to β-glucan exhibited a significant increase in the relative expression of genes linked to chloroplast development and photosynthesis. Additionally, we identified a total of 821 differentially expressed genes (DEGs) between rice plants treated with water and those treated with 50 mg/L of β-glucan, with 373 genes showing upregulation and 448 showing downregulation. Evaluations from the volcano plot, Gene Ontology (GO) functional annotation, and enrichment analysis indicated that these DEGs were mainly enriched in biological processes associated with carbohydrate metabolism and biomass regulation. This study concludes that β-glucan effectively enhances rice growth by improving photosynthetic efficiency. The results provide important insights into the application of exogenous β-glucan for boosting rice productivity.
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产品关联
DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2026
·
IF 3.4
Wen Lei,Li Yuan,Wu Ting,Yu Yue,Wu Yong,Wang Yue,Wang Deguo
Macrophage polarization into M 1 and M 2 phenotypes plays critical roles in homeostasis and disease pathogenesis. M 2 macrophages are characterized by high expression of the mannose receptor (CD206), which specifically binds and internalizes mannosylated ligands. In this study, we developed a novel fluorescent probe by conjugating Rhodamine B (RhB) to mannan isolated from Tremella fuciformis (RhB-mannan). The probe was thoroughly characterized by FTIR, NMR, and fluorescence spectroscopy. Using Raw 264.7 macrophages polarized to M 1 (LPS) or M 2 (IL-4) phenotypes, we demonstrated that the RhB-mannan probe selectively labels M 2 cells, correlating with CD206 expression confirmed by immunofluorescence. The specificity of the probe was further validated by competitive inhibition with free mannose, which abolished the fluorescent labeling. This probe provides a valuable tool for live-cell imaging of M 2 macrophages and studying their role in disease progression.
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Meijun Liu,Fengzhi Qiao,Shaolei Wang,Wenhao Ding,Shichao Xuan,Cristabelle De Souza,Muhammad Asif Javaid,Zhe Zhang,Huaxi Yi,Lanwei Zhang,Kai Lin
This study presents a novel oral delivery system for food-derived angiotensin I-converting enzyme (ACE) inhibitory peptides by integrating β-glucan-functionalized liposomes with microfluidic encapsulation and receptor-mediated targeting. Two peptides, RADHPFL and YAEERYPIL derived from egg white proteins, were encapsulated into liposomes (composed of lecithin, cholesterol, and DSPE-β-glucan) via a controlled microfluidic self-assembly process. Encapsulation significantly improved the gastrointestinal stability of the peptides and preserved their ACE inhibitory activity compared to free peptides. β-Glucan was grafted onto the liposomal surface to enable specific targeting of intestinal microfold (M) cells through interaction with the Dectin-1 receptor, thereby facilitating enhanced uptake in Peyer’s patches. In spontaneously hypertensive rats, both single-dose and continuous administration of β-glucan-functionalized liposomes resulted in superior antihypertensive effects compared to free peptides and unmodified liposomes. Interestingly, the antihypertensive efficacy was primarily attributed to modulation of the renin-angiotensin system, improved renal and cardiac function, and amelioration of endothelial dysfunction. These findings underscore the potential of the natural polysaccharide β-glucan as a targeting ligand in liposomal systems and highlight the utility of carbohydrate-based materials for enhancing the oral bioavailability and physiological efficacy of bioactive peptides. The proposed strategy offers a promising platform for the development of functional foods or nutraceuticals for hypertension management.
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）