Translational Oncology
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2022
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IF 4.803
Yongxiu Du,Yanlan Yang,Wenbo Zhang,Chenxi Yang,Pu Xu
Oral squamous cell carcinoma (OSCC) is a usual oral cancer. Therefore, it's essential to identify targets for its early diagnosis and therapy. This research aimed to explore the roles of human β-defensin-3 (hBD-3) and nuclear factor-kappa B (NF-κB) p65 in the pathogenesis and progression of OSCC. The connection between NF-κB p65 and the carcinogenesis of oral cancer was analyzed by immunohistochemical staining. The relative expressions of hBD-3 and NF-κB p65 in OSCC cells were evaluated by qRT-PCR and Western blot. Afterward, hBD-3 was knocked down, and NF-κB p65 was overexpressed. The cell viability and invasion were tested via CCK-8 and Transwell experiment, and the expression of hBD-3, NF-κB p65, and its downstream molecules was evaluated by Western blot. The expression of NF-κB p65 was increased with the aggravation of the oral submucosal fibrosis. HBD-3 and NF-κB p65 were high-expressed in OSCC cells. The viability and invasion abilities of OSCC cells that knocked down hBD-3 were markedly decreased, while they were restored by the overexpression of NF-κB p65. The expressions of NF-κB p65 and c-myc were diminished while IκB and p21 were raised with the knockdown of hBD-3. After overexpression of NF-κB p65, the expression of hBD-3 and IκB did not change markedly, while c-myc was increased and p21 was decreased dramatically. HBD-3 and NF-κB p65 facilitate the proliferation and invasion of OSCC cells, and hBD-3 may promote this process by governing the expression of NF-κB p65 and its downstream c-myc and p21.
研究领域:
肿瘤学
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分子生物学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
POULTRY SCIENCE
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2023
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IF 4.4
Lili Zhang,Yutong Hou,Zhanbang Ma,Jinjin Xie,Jiahui Fan,Yaru Jiao,Fangfang Wang,Zongxi Han,Shengwang Liu,Deying Ma
ABSTRACT Vitamin A is a fat-soluble vitamin that is a crucial mediator of the immune system. In this study, we evaluated the effect of oral vitamin A supplementation on host immune responses to infectious bronchitis virus ( IBV ) infection in chickens. Forty 1-day-old specific pathogen-free ( SPF ) chickens were fed a basal diet and randomly divided into 2 groups ( n = 20 birds per group). Chickens in the experimental group were treated orally with vitamin A (dissolved in 0.1 mL soybean oil, at a dose of 8,000 IU per kg diet) daily. Birds in the control group were orally administered 0.1 mL soybean oil without vitamin A until 21 d of age. On d 21 after birth, all chickens were infected with 0.1 mL of 10 6.5 50% median embryo infectious dose of a pathogenic IBV strain (CK/CH/LDL/091022) by intraocular and intranasal routes. The results demonstrated that oral vitamin A supplementation did not affect the clinical course of disease and growth performance of SPF chickens. However, vitamin A supplementation increased the IBV-specific IgG serum levels and decreased the viral load in some tissues of IBV-infected chickens. In addition, the results demonstrated that vitamin A supplementation decreased the expression levels of most immune-related molecules in some tissues of IBV-infected chickens. Vitamin A supplementation decreased the mRNA expression levels of some avian β-defensins ( AvBD2, 3, 6, 7, 11 , and 13 ) and increased the expression levels of AvBD9 and AvBD12 in some tissues of IBV-infected chickens. Similarly, vitamin A supplementation decreased the mRNA expression levels of some cytokines (interferon-γ, interleukin-1β [ IL-1β ], and IL-6 ) and increased the mRNA expression levels of IL-2 in some tissues of IBV-infected chickens. Furthermore, vitamin A supplementation decreased the mRNA expression levels of myeloid differentiation primary response protein 88, nuclear factor-κB p65, toll-like receptor 3, toll-like receptor 7 , and CD4. In summary, the present study suggests that vitamin A supplementation enhances the immune function of SPF chickens against IBV infection by inhibiting viral replication, increasing the IBV-specific antibody titer, and suppressing the excessive inflammatory responses to IBV infection.
研究领域:
免疫学
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兽医学
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微生物学
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家禽科学
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产品关联
DOI
PubMed
高影响因子
Food Science and Human Wellness
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2022
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IF 8.022
Yu Ma,Ming Liu,Donghui Li,Jie Li,Zixin Guo,Yunjun Liu,Shengnan Wan,Yixiang Liu
The numerous health benefits of olive oil are widely known, however, it also provides anti-allergic properties that have not yet been fully defined. In this study, the anti-allergic activity of olive oil was evaluated by analyzing the clinical symptoms and immune-related factors in BALB/c mice that had ingested 600 mg/(kg∙day) olive oil for two weeks prior to the evaluation. An allergy model was subsequently constructed for analysis, the results of which showed that the olive oil reduced the scores of allergic symptoms in the mice, and up-regulated the hypothermia and the decline in the immune organ index. Moreover, fewer allergy-related cytokines and reduced intestinal inflammation was discovered in the olive oil-treated group. In addition, analysis of intestinal mucosal immune-related factors revealed that the olive oil promoted the expression of intestinal tight junction proteins (Claudin-1, Occludin, and ZO-1) and IL-22, and helped maintain the integrity of the intestinal epithelial physical barrier. Increased levels of mucin 2 and β -defensin were also found in the intestinal mucus of the olive oil-treated mice. These findings suggest that the oral administration of olive oil effectively attenuated the ovalbumin-induced allergic immune response in the mice, and had a positive effect on intestinal epithelial mucosal immunity.
研究领域:
免疫学
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胃肠病学
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营养学
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产品关联
DOI
高影响因子
JOURNAL OF INVESTIGATIVE DERMATOLOGY
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2023
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IF 6.5
Hong Zhou,Xi Tan,Guozhong Chen,Xinxin Liu,Aiping Feng,Zhi Liu,Wei Liu
Atopic dermatitis (AD) is a chronic inflammatory cutaneous disorder in which the skin is affected by microbial dysbiosis . The role of commensal skin microbiota in AD is of great interest. Extracellular vesicles (EVs) are important regulators of skin homeostasis and pathology. The mechanism of preventing AD pathogenesis through commensal skin microbiota–derived EVs remains poorly understood. In this study, we investigated the role of commensal skin bacterium Staphylococcus epidermidis– derived EVs (SE-EVs). We showed that SE-EVs significantly decreased the expression of proinflammatory genes ( TNFα , IL1β , IL6 , IL8 , and iNOS ) through lipoteichoic acid and increased the proliferation and migration of calcipotriene (MC903)-treated HaCaT cells. Furthermore, SE-EVs increased the expression of human β-defensins 2 and 3 in MC903-treated HaCaT cells through toll-like receptor 2, enhancing resistance to S. aureus growth. In addition, topical SE-EV application remarkably attenuated inflammatory cell infiltration (CD4 + T cells and Gr1 + cells), T helper 2 cytokine gene expression ( Il4 , Il13 , and Tlsp ), and IgE levels in MC903-induced AD-like dermatitis mice. Intriguingly, SE-EVs induced IL-17A + CD8 + T-cell accumulation in the epidermis, which may represent heterologous protection. Taken together, our findings showed that SE-EVs reduced AD-like skin inflammation in mice and may potentially be a bioactive nanocarrier for the treatment of AD.
研究领域:
皮肤科
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免疫学
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微生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
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