LANGMUIR
·
2024
·
IF 3.7
Xiaonan Li,Jing Li,Xiyu Jia,Nian Liu,Xinrui Gao,Zuoqin Zhou,Wei Yang,Bing Wei,Zheng Yang,Suhua Fan,Hai Wu,Hui Zhang
The magnetic composite gel bead (Fe3O4–C@SA GB) adsorbent was prepared by sodium alginate (SA) crosslinking with pitaya peel-derived porous carbons (PPDPCs) and magnetic iron oxide nanoparticles (Fe3O4 NPs). The adsorption effects of Fe3O4–C@SA GBs on heavy metal ions (HMIs) and 17 β-estradiol (E2) in water are evaluated by classical kinetic models and isotherm models. The pseudo-second-order kinetic model shows that Fe3O4–C@SA GBs have maximum adsorption capacities of 9.62, 7.50, and 13.61 mg/g for Cu (II), Cd (II), and Pb (II), respectively. Meanwhile, the highest adsorption performance of the synthesized gel beads to E2 is of ca. 276.3 mg/g. In addition, the Fe3O4–C@SA GBs can still maintain a high level of adsorption efficiency after five adsorption cycles, displaying economic efficiency and reusability. Hence, this work provides useful insights into the efficient adsorption elimination of pollutants in sewage and the corresponding adsorption mechanisms.
研究领域:
环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Minghui Cai,Haixu Yao,Mengnan Li,Yan Yan,Yang Cao,Jiaxin Li,Huiyu Su,Wenwen Zhang,Hui Zhu,Feiyun Cui
β-estradiol is one of the most active hormones in steroids, and it plays a vital role in the human reproductive and non-reproductive systems. Compared with other methods for detecting β-estradiol, electrochemical biosensors are economical, simple, sensitive, and rapid. Herein, spinel copper ferrite nanoparticles and laser-induced graphene (LIG) were employed to functionalize screen-printed carbon electrodes (SPCE) for the first time, and it demonstrated that they have a synergistic effect on the catalysis of β-estradiol. Differential pulse voltammetry (DPV) was used to detect the changes in the peak current generated by the oxidation of β-estradiol. The nanoparticles were characterized by Fourier infrared spectroscopy, transmission electron microscope, scanning electron microscopy, X-ray photoelectron spectroscopy (XPS), and X-ray Diffraction (XRD), and the composition elements and morphology of the nanoparticles were explained. The linear range of β-estradiol detected by this method is 0.1 ∼ 100 μM, R 2 = 0.9992, and the detection limit is 3 nM. The sensor has good selectivity, reproducibility, repeatability, stability, and anti-interference performance. In rat serum, the recovery of the sensor at 100, 10, and 1 μM β-estradiol was 105 % ∼ 110 %, with relative standard deviations of 2.3 % ∼ 4.0 % (N = 3). This study shows that the sensor can be applied to detect actual samples and has potential application value for female reproductive-related diseases.
研究领域:
生物医学工程
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分析化学
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材料科学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Weilu Liu,Haifeng Li,Shangmin Yu,Jiaxing Zhang,Weihua Zheng,Liting Niu,Gengen Li
In this work, we reported the synthesis of 3, 6-diamino-9-ethylcarbazole and its application as a new monomer for preparation of molecularly imprinted polymer (MIP) electrochemical sensor . The as prepared MIP sensor exhibited ultrahigh sensitivity and selectivity for the detection of 17-β-estradiol in attomolar levels (1 × 10 –18 mol L −1 ). The sensor works by detecting the change of the interfacial impedance that is derived from recognition of 17-β-estradiol on the MIP layer. The MIP sensor based on 3, 6-diamino-9-ethylcarbazole monomer revealed better performance than that of unmodified carbazole monomer. The monomer/template ratio, electropolymerization scanning cycles, and the incubation pH values were optimised in order to obtain the best detection efficiency. Under the optimised condition, the MIP sensor exhibits a wide linear range from 1 aM to 10 μM (1 × 10 –18 ̶ 1 × 10 −5 mol L −1 ). A low detection limit of 0.36 aM (3.6 × 10 –19 mol L −1 ) and a good selectivity towards structurally similar compounds were obtained. The proposed MIP sensor also exhibits long-term stability and applicability in human serum samples. These advantages enabled this MIP sensor to be a promising alternative of electrochemical sensor and may be extended to detection of other endogenous compounds .
研究领域:
分析化学
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生物医学工程
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环境科学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 130
·107670-107679
·
IF 3.84
Shoulian Wei,Jianwen Li,Yong Liu
Magnetic mesoporous nanoparticles of Fe3O4@mSiO2 were prepared through co-precipitation and tetraethoxysilane hydrolysis at 60 °C and pH 10 with hexadecyl trimethyl ammonium bromide as the template. Hydroxypropyl β-cyclodextrin was modified onto Fe3O4@mSiO2 under sodium citrate and N2 protection via an ultrasound process to increase the catalytic efficiency. The prepared nanoparticles were characterized by Fourier transform infrared spectrometry, X-ray diffraction, scanning electron microscopy and a peroxidase-like activity assay. The results showed that the prepared nanoparticles have magnetic properties, peroxidase-like activity and a loose spherical cluster structure. Compared with Fe3O4@mSiO2 nanoparticles, Fe3O4@mSiO2@HP-β-CD nanoparticles exhibit higher catalytic ability toward both H2O2 and 3,3′,5,5′-tetramethylbenzidine. Fe3O4@mSiO2@HP-β-CD nanoparticles can catalyse β-estradiol (β-E2) oxidation in the presence of H2O2 and be used as a colourimetric sensor for indirect detection of β-E2. A good linear relationship was obtained from 0.8 μM to 16 μM. The limit of detection of the proposed method was 0.2 μM. The visual method was successfully used in the analysis of β-E2 in commercial tablets and animal feeds, with recovery ranging from 92.6% to 110%.
研究领域:
生物化学
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分析化学
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材料科学
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产品关联
DOI
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Leyuan Chen,Aijiao Yuan,Dapeng Zhang,Wenjing Xie,Hanyong Peng
Detecting β-estradiol (E2) in environmental monitoring is a complex task due to its status as a significant environmental contaminant. The detection methods require precision, sensitivity, and the ability to be conducted on-site without expensive instrumentation. Herein, we developed a novel approach using E2 aptamer assembled spherical nucleic acids (SNAs), which combines the sensitivity of fluorescence and the simplicity of colorimetry. Initially, a fluorescein (FAM)-labeled DNA aptamer is attached to the surface of gold nanoparticles (AuNPs) through hybridization with thiol-labeled DNA, resulting in fluorescence quenching. However, when E2 is present, the aptamer specifically binds to it, displacing from the thiol-DNA and releasing from the AuNP's surface. This leads to the recovery of fluorescence, allowing for quantitative detection of E2 by measuring the increase in fluorescence signal. Additionally, E2 detection can also be achieved visually using ultraviolet light. For colorimetric analysis, we introduce another set of AuNPs modified with thiol-DNA complementary to the DNA remaining on the surface of the previous AuNPs. When E2 triggers the release of the aptamer, the DNA on both AuNPs hybridized to each other, causing the aggregation of AuNPs and resulting in a distinct color change from red to purple. The detection limits for fluorescence and colorimetric analyses are 1 nM and 5 nM, respectively. We successfully applied this biosensing strategy to determine E2 concentrations in tap water and serum samples. Furthermore, our assay exhibits high selectivity towards E2 over other estrogens. Overall, this innovative approach provides an effective and versatile method for convenient on-site monitoring of E2.
研究领域:
分析化学
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环境科学
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纳米技术
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生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
·
2025
·
IF 2.5
Xiaoli Liu,Shan Wang,Chuangfu Kuang,Yuwen Deng,Shuaicai Yuan,Juying Zou
We aim to explore the key metabolic components and underlying mechanisms of the Bushen Huoxue Formula (BH) in treating Osteoarthritis (OA). The mouse knee OA model was constructed using the destabilization of the medial meniscus method. OA mice were orally administered the BH. Mouse cartilage damage was assessed. High performance liquid chromatography-tandem mass spectrometry (HPLC-MS), network pharmacology analysis and molecular docking were employed to analyze the serum metabolite components and target protein of BH. After lipopolysaccharide (LPS) treatment, different concentrations of β-Estradiol 3-acetate were added to primary chondrocytes. Flow cytometry was utilized for detecting cell apoptosis. The Ubiquitin-Specific Protease 13 (USP13)/Toll-like Receptor 4 (TLR4)/Myeloid Differentiation Primary Response Protein 88 (MYD88)/NF-κB pathway and the TLR4 ubiquitination levels were assessed using immunological quantification and biochemical methods. Relative to normal mice, OA mice exhibited decreased knee joint cartilage thickness and increased inflammatory damage. BH treatment reversed these effects. Furthermore, BH enhanced TLR4 ubiquitination. Estradiol acetate was identified as the metabolic component of BH that alleviates OA. Estradiol acetate and its subtype molecule β-Estradiol 3-acetate could bind to the USP13 protein. The β-Estradiol 3-acetate concentration-dependently decreased the elevated levels of USP13, TLR4, MYD88, p-p65/p65 in chondrocytes induced by LPS, while increasing the TLR4 ubiquitination. β-Estradiol 3-acetate reversed LPS-induced chondrocyte apoptosis and elevation of inflammatory factors. Moreover, USP13 overexpression abolished the protective effects of BH and β-Estradiol 3-acetate against LPS-induced chondrocytes. In Conclusion, the BH metabolite β-Estradiol 3-acetate promotes TLR4 ubiquitination to relieve inflammation and apoptosis in OA chondrocytes by inhibiting USP13.
研究领域:
药理学
·
中医中药
·
分子生物学
·
风湿病学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Yan Huang,Maowen Chen,Yiding Shen,Xinkun Shen,Menghuan Li,Yanan Li,Yuan Liu,Kaiyong Cai,Zhong Luo,Yan Hu
Osteoporosis is a common bone disease characterized by the systemic disruption of hone homeostasis, where the imbalance between bone resorption and formation causes deterioration of bone structure and bone mass loss. Targeted restoration of bone hemostasis has thus become a promising strategy for osteoporosis treatment. For this purpose, we report a bone-targeted nanoformulation based on CaCO 3 nanomaterials, which could be activated in the acidic osteoporotic microenvironment and cooperatively regulate the activity of osteoclasts and osteoblasts to reverse bone deterioration. Herein, a clinical antiosteoporosis drug β-estradiol (E2) is first biomineralized using CaCO 3 to form physiologically stable nanocores. Meanwhile, azido groups are introduced onto MC3T3-E1 cells through incubation with AC 4 ManNAz. The azido-tagged MC3T3-E1 cell membranes are extracted for the camouflaging of the CaCO 3 @E2 nanocores to enhance their in vivo stability and availability, while also enabling the surface modification of bone-targeting dibenzocyclooctyne-tagged alendronate moieties via copper-free click reaction. After the selective deposition into acidic osteoporotic bone tissues, the CaCO 3 @E2 cores would be spontaneously degraded to release E2 and Ca 2+ ions, which would systematically inhibit the activity of osteoclasts while promoting osteoblast-mediated new bone formation, eventually ameliorating the osteoporosis symptoms. This study offers an effective approach for osteoporosis treatment in the clinics.
研究领域:
药理学
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纳米医学
·
骨骼生物学
·
药物递送系统
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产品关联
DOI
高影响因子
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
·
2022
·
IF 5.011
Liangliang Zhu,Hui Lv,Ling Xiao,Yanyao Hou,Wenjuan Li,Guangbo Ge,Chunzhi Ai
β-estradiol (β-E2) and α-estradiol (α-E2) act as an endo- and an exon-estrogen in humans, respectively. There is a structural variation in C17-OH configuration of the two estrogens. UDP-glucuronosyltransferases (UGT) are responsible for termination of activities of a variety of endogenous hormones, clinical drugs, and environmental toxicants. The current study was conducted to investigate the effects of the two estrogens towards catalytic activities of UGTs. It was found that β-E2 could decrease activities of UGT1A9 , − 2B4 and − 2B7, with K i values of a few micro-molars. β-E2 could additionally accelerate the activity of UGT2B17 via promoting enzyme-substrate binding and increasing the turn over number . Comparatively, α-E2 displayed much stronger inhibitory potentials towards UGT2B7 and − 2B4, but showed little influence to UGT1A9 and − 2B17. The K i values for inhibition of UGT2B7 in glucuronidation of different substrates by α-E2 were in a nanomolar range that is only about 1/100–1/50 of β-E2. UGT2B7 structural model was fatherly constructed to explore the mechanism underlying dramatically different inhibition selectivity of the two estrogens. Compared to β-E2, α-E2 formed more hydrophobic and hydrogen-bonded interactions with the residues in the active pocket. It is concluded that the configuration of E2–17-OH determines the inhibitory potentials towards UGTs. The results are useful in better understanding ligand selectivity of UGTs, as well as in further development of α-E2 in health protection.
研究领域:
药理学
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生物化学
·
分子生物学
·
内分泌学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Yulu Jia,Chengyang Zhang,Hongcen Zheng,Guoyang Zhang,Shujuan Zhang
Indirect photodegradation is an important pathway for the reduction of steroid estrogens in sunlit surface waters. Nevertheless, the kinetics and mechanisms governing the interaction between coexisting carbonyl compounds and estrogens under visible light (Vis) remain unexplored. This study systematically investigates the Vis-induced photodegradation of 17 β -estradiol (E2) in the presence of five specific carbonyl compounds—biacetyl (BD), acetone, glyoxal, pyruvic acid, and benzoquinone . The results demonstrate that, among these compounds, only BD significantly enhanced the photodegradation of E2 under Vis irradiation (λ > 400 nm). The pseudo-first order photodegradation rate constants ( k 1 ) of E2 in the Vis/BD system were 0.025 min −1 and 0.076 min −1 in ultrapure water and river water, respectively. The enhancing effect of BD was found to be pH-dependent, increasing the pH from 3.0 to 11.0 resulted in a 76% reduction in the k 1 value of E2 in the Vis/BD system. Furthermore, the presence of humic acid , NO 3 − , or HCO 3 − led to an increase of more than 35% in the k 1 value of E2, while NO 2 − exerted a pronounced inhibitory effect, resulting in a 92% decrease. Peroxyacetyl and peroxymethyl radicals, derived from BD in a yield ratio of 9, played a crucial role in the degradation of E2. These peroxyl radicals primarily targeted electron-rich hydroxyl sites of E2, initiating hydroxylation and ring-opening reactions that culminated in the formation of acidic byproducts. Notably, toxicity evaluation indicates that these hydroxylated and acidic products exhibited lower toxicity than the parent compound E2. This study highlights the important role of peroxyl radicals in estrogen degradation within aquatic environment, and also helps to design efficient visible light-responsive photo-activators for the treatment of estrogen-contaminated waters.
研究领域:
环境科学
·
化学
·
水处理
·
毒理学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Abdul Hakeem Deshmukh,Sili Huang,Hongli Wen,Abdur Raheem Aleem,Yaser E. Greish,Mutamed Ayyash,Saleh T. Mahmoud
Consistent exposure to 17β-estradiol (E 2 ) through water and food sources presents considerable health hazards, necessitating the development of sensitive E 2 detection methods. Herein, an E 2 nanosensor based on Förster resonance energy transfer (FRET) was developed by immobilizing amino-SiO₂-modified upconversion nanoparticle (UCNP) core–shell structures functionalized with E 2 -specific aptamers on graphene oxide (GO) nanosheets. The aptamers adsorbed onto GO via π–π stacking, facilitating the FRET-induced upconversion luminescence quenching. Upon E 2 addition, the aptamers detached from GO, restoring the UCL intensity. The as-developed nanosensor exhibited a strong linear response to E 2 concentrations in the range of 10–200 ng/mL and a detection limit of 6.39 ng/mL. Furthermore, the high recovery rates (88–98 %) of the sensor validated its reliability. The fabricated sensor demonstrated excellent potential for the accurate detection of E 2 in aqueous environments.
研究领域:
环境科学
·
分析化学
·
纳米技术
·
生物传感器
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2025
·
IF 5.6
Xiutao Yang,Zhijie Luo,Yuying Wang,Shaoqin Zhang,Guopeng Zhu,Chunhui Wang,Quanying Wang,Hongwen Yu
The sensitive and accurate detection of 17β-estradiol (E2) is crucial for assessing environmental and food safety risks. To this end, we developed a novel electrochemical hybrid sensor by integrating an aptamer (Apt) with molecularly imprinted polymers (MIP) on a glassy carbon electrode (GCE) modified with AgNPs/NiMOF-Ni composites. This unique design leverages the large conductive surface of AgNPs/NiMOF-Ni to immobilize the recognition elements, while the Apt-MIP dual-recognition system ensures exceptional target specificity. The fabricated sensor demonstrated high sensitivity, excellent selectivity, and satisfactory stability for E2 detection. It achieved a broad linear range from 1.0 × 10⁻¹⁵ M to 1.0 × 10⁻⁶ M and an ultra-low detection limit of 0.44 fM (S/N = 3). When applied to spiked water and milk samples, the sensor delivered reliable recoveries of 95.2% - 109% with a relative standard deviation (RSD) ≤ 4.8%. These results affirm the strong potential of our hybrid sensor for the practical monitoring of trace E2 contamination.
研究领域:
环境科学
·
分析化学
·
传感器与生物传感器
·
食品安全
·
纳米材料
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Lihong Su,Luliang Wang,Lei Zhao,Yushen Liu,Chengke Wang,Chen Shao,Lu Liu,Daohong Zhang,Ibrahim A. Darwish
The development of advanced adsorbents enabling rapid and sensitive detection of endocrine-disrupting bisphenols (BPs) is essential for safeguarding ecosystems and human health. Covalent organic frameworks (COFs) hold great promise for the adsorption of BPs, but conventional rigid aromatic architectures suffer from limited molecular recognition capability. Herein, we design a hydroxyl-functionalized flexible aliphatic COF (TFT-TAH COF) via the polymerization of 2,3-dihydroxydicarbohydrazid (TAH) and 1,3,5-tris(4-formyl-phenyl) triazine (TFT) for efficient extraction and sensitive detection of BPs (BPA, BPF, BPAF, and BPF). The flexible aliphatic linker exposes more active sites, thereby displaying excellent BPs adsorption capacity (654–1111 mg g −1 ) and rapid adsorption kinetics (equilibrium within 30 s). TFT-TAH COF gave good affinity for BPs through the synergistic effects of size matching, hydrogen bonding, and π-π interactions. In addition, a novel analytical method was developed for BPs determination by coupling TFT-TAH COF-based dispersive solid-phase extraction with high-performance liquid chromatography, achieving low detection limits of 0.03–0.1 ng mL −1 and satisfactory precision with relative standard deviations≤5.7 %. Moreover, recoveries for the spiked lake, river, drinking water, bottled water, and milk samples ranged from 89.8 to 101.6 %. This work pioneers an innovative approach to fabricating flexible aliphatic-linked COFs for environmental monitoring and food safety.
研究领域:
环境化学
·
材料科学
·
分析化学
·
食品安全
·
可持续技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）