CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Hao Wu,Xiaoxiao Li,Hangyan Ji,Birte Svensson,Yuxiang Bai
High substrate viscosity during the production process suppressed the formation of gamma-cyclodextrin (γ-CD) from starch . Although liquefaction can be carried out by enzymes with high temperature optimum, some CGTases of interest are not suitable due to loss of activity at the gelatinization temperature. In order to apply a gamma-cyclodextrin glucosyltransferase (γ-CGTase) with an optimum temperature of 50 °C to the liquefaction of 10% w/v cassava starch, an improved process combining moderate heat treatment (30 °C increased to 70 °C) with enzymatic treatment was established. The substrate viscosity dropped from 2456 to 23 cP after the pretreatment, and the rate of γ-CD production increased from 10.43 to 26.34%. This process was further applied to the liquefaction of 30% w/v cassava starch, and the pretreated substrate was finally converted into 21.81% γ-CD. In general, evaluation indicators and basic principles of starch pretreatment were established, providing a reference for the production of γ-CD.
研究领域:
生物化学
·
食品科学
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Yanli Zhao,Ziqi Cao,Yanxiang Liang,Zhichen Liang,Keyin Ling,Feiyan Zhou,Changsheng Guo,Yao Qian,Pengbi Liu,Xi Liu,Chunguang Wang,Mengchen Zhang,Qinglei Zhang
Membrane consisting of high-throughput aligned ion channels has been pursued for precise Li + /Mg 2+ separation. Herein, an aligned amino-functionalized γ-cyclodextrin nanofiltration membrane was fabricated via a customized interfacial polymerization technique, where the orientation of cyclodextrin ion channels was elaborately tailored by controlling the reaction conditions. The as-fabricated membrane featured ultra-thin selective layer of ∼ 26 nm, average pore size of ∼ 0.72 nm, and ultra-stable positive surface charges. In particular, the aligned intrinsic cyclodextrin cavities in the membrane offered vertical shortcuts for preferential Li + transport. Taking advantage of these, superior membrane permselectivity was achieved with the water permeance of 6.5 L·m −2 ·h −1 ·bar −1 and the Li + /Mg 2+ separation factor up to 22.5, highlighting the great potentials in lithium extraction and other ion separations. Given its simplicity and generality, this customized interfacial polymerization strategy holds great promise for manipulating aligned transport channels in thin film composite membranes, thereby enhancing their performance in a wide range of applications including, but not limited to, desalination, water purification, resource extraction, and energy conversion.
研究领域:
材料科学
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化学工程
·
环境科学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Yan Chen,Li Liao,Jin Liu,Hongxia Gao,Xing Chen,Lexiang Zhang,Liqiang Zou
Vanillin is widely used as a food flavoring agent for its pleasant aroma and biological properties, but its volatility during high-temperature processing limits its effectiveness. In this study, ternary complex microparticles consisting of vanillin/γ-cyclodextrin/dextran (V/C/D) were prepared and applied in bread manufacturing. The retention rate of vanillin during bread baking increased from 44.25% to 69.92% as the proportion of dextran in the complex microparticles (V/C/D 1:9:0-V/C/D 1:5:4) was enlarged. Furthermore, enhanced vanillin retention within the bread improved its resistance to oxidation and spoilage during storage. Incorporating vanillin-complexed microparticles into bread delayed increases in acid value, peroxide value, and total colony count during storage at 37 °C, extending the bread's shelf life from 4 to 6 days. Vanillin retained in the bread inhibited microbial growth, significantly reducing the relative abundance of Bacillus (from 81.98% in blank bread to 7.54% in V/C/D 1:5:4) and Aspergillus flavus (from 99.94% in blank bread to 83.00% in V/C/D 1:5:4) during storage. The results from this research could offer valuable insights into enhancing the quality of natural flavorings and baked goods.
研究领域:
食品科学
·
微生物学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2024
·
IF 5.4
Xiaowei Ding,Xuerong Luo,Imran Mahmood Khan,Lin Yue,Yin Zhang,Zhouping Wang
Geraniol (Ger) is an essential oil molecule with excellent biological activity. High hydrophobicity and volatility limit its practical application. Cyclodextrins (CDs) are water-soluble cyclic oligosaccharides with hydrophobic cavities. Physical encapsulation of CDs to improve the solubility and stability of essential oil molecules is not satisfactory. Therefore, this study synthesized the γ-CD derivative (γ-CD-Ger) by grafting Ger onto γ-CD using a bromide-mediated method. Compared to the inclusion complexes (γ-CD/Ger) formed by both, the derivatives exhibit better solubility and thermal stability. The derivative has better antibacterial activity when the ratio of γ-CD to Ger was 1:2. In addition, the derivatives did not exhibit cytotoxic and hemolytic properties. These results indicate that this research provides a water-soluble antibacterial agent with a wide range of promising applications and offers new ideas for the application of alcohol hydrophobic molecules in aqueous systems.
研究领域:
食品科学
·
药物化学
·
微生物学
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Zou Ying,Zhang Manli,Zhou Dongdong,Chi Chaoxian,Wu Fangling,Ding Chuan-Fan
Vitamin C (L-( +)-ascorbic acid, L-AA) is an essential micronutrient. Its diastereoisomer, D-(-)-isoascorbic acid (D-IAA), is only 5% as active as L-AA. Therefore, it is crucial to identify and characterize the diastereoisomers of ascorbic acid. In this work, a straightforward and direct method using ion mobility-mass spectrometry (IM-MS) was proposed to identify ascorbic acid isomers. Ternary complexes [L-AA + γ-CD + 2Cs-H] + and [D-IAA + γ-CD + 2Cs-H] + , formed by non-covalent interaction of the isomer with the selective agent γ-CD and the metal ion Cs + , were separated in ion drift tubes with a resolution of R p-p as high as 1.398. Meanwhile, comparisons with different CDs and metal ions revealed varying separation efficiencies. Theoretical calculations were conducted to determine the optimal conformations of [L-AA + γ-CD + 2Cs-H] + and [D-IAA + γ-CD + 2Cs-H] + . Conformational analysis highlighted distinct structural differences at the molecular level, providing insight into the mobility separation mechanism of AA via the formation of ternary complexes with γ-CD and metal ions. Additionally, a quantitative analysis for the determination of chiral isomers was established with effective linearity and acceptable sensitivity. The method was successfully applied to assess L-AA/D-IAA content in pharmaceuticals and fruit samples. Graphical
研究领域:
分析化学
·
营养学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2016
·Vol. 34
·期 4
·425-431
·
IF 1.872
Yafei Guo,Yushun Zhang,Jiuling Li,Fen Zhao,Yang Liu,Min Su,Yubo Jiang,Yuqi Liu,Jihong Zhang,Bo Yang,Rui Yang
An inclusion complex of podophyllotoxin (PPT) with γ -cyclodextrin ( γ -CD) was prepared. The behavior, characterization, and water solubility of the inclusion complex were carefully investigated via fluorescence spectroscopy, thermogravimetry, differential scanning calorimetry, X-ray diffraction analysis, and 1 H and 2D nuclear magnetic resonance spectroscopy. Furthermore, antitumor activity to human cancer lines and toxicity in mice were studied. Results showed that the inclusion complex formed in a 1:1 ratio with a considerable apparent stability constant K s (4245.5 L·mol −1 ). Water solubility was considerably improved. In addition, the anticancer activity of the inclusion complex was better than that of cis -platinum (DDP, positive control). Most importantly, the toxicity of podophyllotoxin inclusion complex reduced and became more safety to mice which will be great valuable to research its applications as a kind of antitumor drug to human in the further.
研究领域:
药学
·
肿瘤学
·
药物化学
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DOI
高影响因子
MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2022
·Vol. 223
·期 9
·2200022
·
IF 2.996
Lili Cai,Jiawei Lin,Mingyu Qiao,Jianwei Guo,Huatang Zhang,Sa Liu,Yong-Guang Jia
The multiregulation of aggregation-induced emission (AIE) that responds to external stimuli to finish multifunctions has become a focus of interest in biodiagnosis. Host–guest- recognition is an efficient strategy to introduce switchable, reversible, and other functional properties into AIE molecules. Herein, an amphiphilic tetraphenylethylene (TPE) derivative TPE-PEG is prepared via the simple amidation reaction and chosen as an AIE-based guest polymer. The aggregated state of TPE-PEG is altered and the fluorescence of TPE-PEG can be turned off in the presence of γ -cyclodextrins ( γ -CDs) due to the formation of host–guest complex between TPE units and γ -CDs. TPE units in the cavities of γ -CDs are released free and an aggregated state of TPE-PEG is restored via the guest competition epistasis of bile acid. Meanwhile, α -amylase can hydrolyze the host molecules of γ -CDs, resulting in an aggregated state of TPE-PEG again and turning the fluorescence on subtly. This reversible multiregulation of fluorescence may meet the multirequirements for the diagnosis in vivo due to the biological nature of α -amylase and bile acid.
研究领域:
生物化学
·
分子生物学
·
材料科学
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DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Mengmeng Zhang,Jingbo Liu,Yiding Yu,Wenshuo Ren,Rui Yuan,Xuanting Liu,Xiaomin Shang,Zhiyang Du,Meng-Lei Xu,Ting Zhang
Genistein (GEN) is the most abundant aglycone in soy isoflavones and is a key factor distinguishing soybeans from other food sources. It holds significant nutritional value and exhibits notable biological activity. However, the low water solubility of genistein limits its widespread application. To improve its water solubility, this study prepared γ-cyclodextrin (γ-CD)/GEN inclusion complexes and investigated their mechanism of action in alleviating hydrogen peroxide-induced oxidative stress damage in PC12 cells. The results showed that γ-CD/GEN could be uniformly and stably dispersed in the system. Cell experiments indicated that γ-CD/GEN significantly increased cell survival after treatment with organic hydrogen peroxide, and promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and the expression of glutathione peroxidase 4 (GPx4). It also improved mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) levels, thereby alleviating cell damage caused by oxidative stress. Further Nrf2 knockdown experiments revealed that γ-CD/GEN failed to provide protection, and no protective effect was observed under RSL3 intervention. In conclusion, γ-CD/GEN exerts antioxidant effects by activating the Nrf2/GPx4 pathway, thereby reducing oxidative stress damage induced by organic hydrogen peroxide, providing a theoretical basis for the development of novel antioxidants.
研究领域:
药理学
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分子生物学
·
神经科学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yun-Dong Yin,Lei Yang,Xi-Tong Song,Jun Hu,Fang-Fang Chen,Ming Xu,Zhi-Yuan Gu
The high expression of Spermidine/spermine N1-acetyltransferase (SSAT-1) is an important indicator in early cancer diagnosis. Here, we developed a nanopore-based methodology with γ-cyclodextrin as an adaptor to detect and quantify acetylamantadine, the specific SSAT-1-catalyzed product from amantadine, to accordingly reflect the activity of SSAT-1. We employ γ-cyclodextrin and report that amantadine cannot cause any secondary signals in γ-cyclodextrin-assisted α-HL nanopore, while its acetylation product, acetylamantadine, does. This allows γ-cyclodextrin to practically detect acetylamantadine in the interference of excessive amantadine, superior to the previously reported β-cyclodextrin. The quantification of acetylamantadine was not interfered with even a 50-fold amantadine and displayed no interference in artificial urine sample analysis, which indicates the good feasibility of this nanopore-based methodology in painless cancer prediagnosis. In addition, the discrimination mechanism is also explored by 2-D nuclear magnetic resonance (NMR) and nanopore experiments with a series of adamantane derivatives with different hydrophilic and hydrophobic groups. We found that both the hydrophobic region matching effect and hydrophilic interactions play a synergistic effect in forming a host–guest complex to further generate the characteristic signals, which may provide insights for the subsequent design and study of drug-cyclodextrin complexes.
研究领域:
生物化学
·
医学诊断
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Dehua Wang,Biqian Wei,Yi Zhang,Tongxin Xie,Jing Yu,Yong Xue,Biao Kong,Changhu Xue,Qingjuan Tang
Excessive salt consumption is strongly linked to the chronic diseases development, highlighting the urgent need for effective salt-reduction strategies in food products. However, reducing salt content often compromises both food preservation and flavor. In this study, we hypothesized that curcumin could enhance the flavor perception of sodium chloride while compensating for its antimicrobial effect in reduced-salt applications. To test this, we developed a curcumin/γ-cyclodextrin/sodium chloride complex (IC), using γ-cyclodextrin as a carrier, and evaluated its antimicrobial and flavor-enhancing properties. The complex's formation was confirmed through various analytical techniques. Results from electronic tongue and sensory evaluations demonstrated that the IC enhanced the perception of saltiness and imparted unique aromatic characteristics compared to standard sodium chloride. GC-IMS analysis identified key aroma contributors, such as aldehydes and alcohols. Additionally, antimicrobial tests revealed that the IC efficiently mediated the sono/photodynamic inactivation of Shewanella putrefaciens and Vibrio parahaemolyticus . In summary, the IC offers a multifunctional approach, enhancing flavor, reducing salt, and enabling antimicrobial activity, while promoting practical uses of sono/photodynamic technology in low-sodium foods.
研究领域:
食品科学
·
微生物学
·
营养学
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产品关联
DOI
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 7
·1456
·
IF 4.6
Shuang Gao,Honglei Yan,Yue Xiu,Fengrui Li,Yu Zhang,Ruichi Wang,Lixia Zhao,Fei Ye,Ying Fu
The discovery of efficient and stable nanopesticides with improved water solubility and sustained release effects has become particularly important. Pyrimethanil (Pyr) as a low toxicity fungicide of an aniline pyrimidine group is widely used for the prevention and control of gray mold in crops and ornamental plants, however, poor water solubility hinders its further development. Herein, we use a supramolecular self-assembly process to encapsulate a pyrimethanil in a hydroxypropyl-gamma-cyclodextrin (HPγCD) via electrostatic interactions, thereby constructing the inclusion complex nanofibers. The HPγCD as an environmentally friendly carrier material for pesticide delivery is favorable for facilitating the control efficacy, water solubility, and thermostability with Pyr. The diameter of the prepared inclusion nanofiber is 426.6 ± 82.1 nm. Pyr/HPγCD inclusion complex nanofibers could be completely dissolved in water within 3 s. As predicted, the fungicidal activity of Pyr/HPγCD inclusion complex nanofibers is much higher than that of either Pyr, and the EC 50 value of Pyr/HPγCD inclusion nanofibers is 0.437 μg/mL, which is about half of that of Pyr (0.840 μg/mL). The inclusion strategy achieved by Pyr and HPγCD is important for improving the safety of nanopesticides. This work provides a versatile insight to promote the development of water-based pesticide dosage forms and reduce pesticide losses in agricultural production.
研究领域:
农业科学
·
材料科学
·
植物保护
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Nan Li,Li Qi,Juan Qiao,Yi Chen
A ratiometric, versatile, and selective fluorescent pattern to sense and distinguish proteins on the basis of dissociation of aqueous polymer-pyrene/γ-cyclodextrin (γ-CD) inclusion complexes was developed. First, two kinds of aqueous polymer-pyrene were prepared via atom transfer radical polymerization using pyrene functionalized initiator. Then, the pyrene molecules could be accumulated into γ-CD cavity and form polymer-pyrene/γ-CD complexes, resulting in appearance of excimer emissions. The resultant complexes responded to proteins in two ways: nonmetalloproteins binding to polymer component triggered dissociation of the inclusion complexes, accompanied by alteration of pyrene excimer/monomer emission and ratiometric fluorescent intensity changes; the presence of metalloproteins could quench pyrene excimer/monomer emission because of energy transfer. Moreover, the fluorescent responses of the inclusion complexes to different proteins could be modulated by changing polymer type and chain length, resulting in a tunable selectivity and sensitivity. The proposed fluorescent inclusion complexes could provide a promising platform for sensing proteins.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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