Journal of Environmental Chemical Engineering
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2025
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IF 7.2
Mingrui Geng,Zhizhen Wang,Denghui Wang
Formaldehyde (HCHO), a ubiquitous indoor organic pollutant, severely threatens human health. Developing efficient and low-energy catalytic materials for room-temperature HCHO purification remains an urgent priority. In this work, Fe y MnO x catalysts were synthesized via a facile coprecipitation method. The Fe doping effectively modulated the morphology and crystallinity of δ-MnO 2 while inducing the formation of surface oxygen vacancies, thereby enhancing the capability to generate surface reactive oxygen species. Under a gas hourly space velocity (GHSV) of 150 L·g cat –1 ·h –1 , the catalyst achieved > 99 % removal efficiency for 100 ppm HCHO, along with exceptional stability. In situ DRIFTS reveals the influence of Fe doping on the catalytic reaction pathway for HCHO. This work demonstrates that the Fe doping strategy successfully optimizes the microstructure and surface chemical properties of δ-MnO 2 , resulting in outstanding room-temperature HCHO removal performance, providing valuable insights into defect engineering for optimizing transition metal oxide catalysts.
研究领域:
环境催化
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材料科学
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室内空气净化
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过渡金属氧化物
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缺陷工程
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高影响因子
MICROCHEMICAL JOURNAL
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2026
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IF 5.2
Yiyang Fan,Xiaoyi Yang,Xin Xu,Tong Shen,Ze Zhang,Fan Wang,Hongzhi Pan,Dong Chang
A CRISPR-responsive DNA hydrogel gates δ-MnO 2 nanozyme release. • RPA–Cas12a recognition enables detection of five common uropathogens. • The assay achieved LODs of 2.44–45.47 copies/μL across targets. • Parallel channels showed specificity, reproducibility, and 20-day stability. • KernelRidge-RBF provided the lowest prediction error among nine evaluated regression models. Urinary tract infection (UTI) requires rapid and accurate pathogen identification, yet conventional culture-based methods are time-consuming and many molecular assays remain dependent on sophisticated instrumentation. Here, we developed a multiplex visual biosensing platform for uropathogen detection by integrating recombinase polymerase amplification (RPA), CRISPR/Cas12a recognition, a CRISPR-responsive DNA hydrogel gate, and a δ-MnO 2 nanozyme-mediated colorimetric module. In this system, target-derived amplicons activate pathogen-specific Cas12a, which degrades the DNA hydrogel and releases encapsulated δ-MnO 2 , thereby catalyzing the TMB/H 2 O 2 reaction to produce a visible and spectrophotometrically measurable signal. The platform was applied to five representative uropathogens, including Escherichia coli , Klebsiella pneumoniae , Pseudomonas aeruginosa , Enterococcus faecium , and Enterococcus faecalis . Under optimized conditions, the assay showed good linearity for all five targets, with R 2 values of 0.9955, 0.9958, 0.9948, 0.9974, and 0.9956, respectively, and limits of detection of 2.60, 3.15, 2.44, 20.40, and 45.47 copies/μL, respectively. The platform also exhibited satisfactory specificity, multiplexing capability, reproducibility, and storage stability. To improve quantitative interpretation, an image-recognition- and machine-learning-assisted workflow was further introduced, in which KernelRidge-RBF showed the best overall performance among the tested models. Clinical urine sample testing further supported the applicability of the platform, with AUC values of 1.000, 0.976, 0.994, 0.947, and 0.939 for the five target pathogens, respectively. Collectively, the RPA-Cas12a-DNA hydrogel-δ-MnO 2 platform enables visual and parallel multi-target detection of uropathogens within approximately 3 h and provides a proof-of-concept framework for the further development of portable nucleic acid biosensors. Download: Download high-res image (194KB) Download: Download full-size image
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高影响因子
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