ACS Biomaterials Science & Engineering
·
2025
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IF 5.5
Xinyun Pu,Xu Peng,Shubin Shi,Shaoxiong Feng,Xu Wei,Xi Gao,Xixun Yu
Valvular heart disease (VHD) poses a significant threat to human health, and the transcatheter heart valve replacement (THVR) is the best treatment for severe VHD. Currently, the glutaraldehyde cross-linked commercial bioprosthetic heart valves (BHVs) remain the first choice for THVR. However, the cross-linking by glutaraldehyde exhibits several drawbacks, including calcification, inflammatory reactions, and difficult endothelialization, which limits the longevity and applicability of BHVs. In this study, λ-carrageenan with anticoagulant function was modified by carboxymethylation into carboxymethyl λ-carrageenan (CM-λC); subsequently, CM-λC was used as a cross-linking agent to stabilize decellularized bovine pericardial tissue through amide bonds formed by a 1-(3-(Dimethylamino)propyl)-3-ethylcarbodiimide/N-Hydroxysuccinimide (EDC/NHS)-catalyzed reaction between the amino functional groups within pericardium and the carboxyl group located on CM-λC. Lastly, the inclusion complex (CD/Rutin) (formed by encapsulating the rutin molecule through the hydrophobic cavity of the mono-(6-ethylenediamine-6-deoxy)-β-cyclodextrin) was immobilized onto above BHVs materials (λCar-BP) through the amidation reaction. The treated sample exhibited mechanical properties and collagen stability similar to those of GA-BP, except for improved flexibility. Because of the presence of sulfonic acid groups and absence of aldehyde group as well as the Rutin release from CD/Rutin immobilized onto BHVs, the hemocompatibility, anti-inflammatory, HUVEC-cytocompatibility, and anticalcification properties, of the CM-λC-fixed BP modified with CD/Rutin was significantly better than that of GA-BP. In summary, this nonaldehyde-based natural polysaccharide cross-linking strategy utilizing the combination of CM-λC and CD/Rutin provides a novel solution to obtain BHVs with durable and stable anticoagulant, anticalcification, and anti-inflammatory properties, and has a wide range of potential applications in improving the various properties of BHVs.
研究领域:
医学材料学
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心血管病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Dongling Qiao,Yuyan Zhang,Farong Sun,Michelle Yoo,Guohua Zhao,Binjia Zhang
Enhancement mechanism of ι-carrageenan on the network structure and gel-related properties of soy protein isolate (SPI)/λ-carrageenan system was investigated. Adding ι-carrageenan decreased SPI particles' size in nano-scale, reduced hydrophobic interactions by suppressing exposure of hydrophobic groups, and increased the disulfide bonds in SPI. With rising ι-carrageenan content (< 0.55 %), the interaction between ι-carrageenan and SPI was enhanced mainly through electrostatic interaction and hydrogen bond. Meanwhile, SPI particles were reduced and their stacking compactness was increased as well. These contributed to the improvement of network structure and thus increased weakly bonded water from 97.7 to 98.5 %, water holding capacity from 55.29 % to 61.70 % and gel hardness from 0.53 to 1.30 N. Higher ι-carrageenan content (> 0.55 %) induced micro-phase separation as shown by CLSM images, and led to reduction of gel hardness. These results favor the rational design and application of SPI-based gel systems with desired practical properties.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Jianmiao Guo,Yanghui Liu,Yingtao Li,Fangzhou Li,Feng Huang
With reference to the organization of the human brain nervous system, a hardware-based approach that builds massively parallel neuromorphic circuits is of great significance to neuromorphic computing. The Bienenstock–Cooper–Munro (BCM) learning rule, which describes that the synaptic weight modulation exhibits frequency-dependent and tunable frequency threshold characteristics, is more compatible with the working principle of neuromorphic computing systems than spike-timing-dependent plasticity. Therefore, it is interesting to simulate the BCM learning rule on solid-state synaptic devices. Here, we have prepared λ-carrageenan (λ-car) electrolyte-gated oxide synaptic transistors, which exhibit good transistor performances, including a low subthreshold swing of 125 mV/dec, an on/off ratio larger than 106, and a mobility of 9.5 cm2 V–1 s–1. By modulating the initial channel current and spike frequency, the simulation of the BCM rule was successfully realized. The competitive relationship between the drift of protons under an electric field and the spontaneous diffusion of protons can explain this mechanism. The proposed λ-car-gated synaptic transistor has a great significance to neuromorphic computing.
研究领域:
神经科学
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材料科学
·
电子工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Hui Wang,Hongtong Wang,Xiaoting Zhang,Mingyan Dong,Fang Huang,Ping Li,Bo Tang
Arthritis is an inflammatory and autoimmune disease seriously affecting the quality of a patient’s life, and its pathogenesis remains obscure. Mitochondrial oxidative stress is involved in the patho-physiology of arthritis. Cystein (Cys) plays a crucial role as a free radical scavenger in oxidative stress, and thus decreased mitochondrial Cys might be observed in mice with arthritis. To verify this inference, we report a two-photon imaging probe for fluorescence measurement of mitochondrial Cys with high selectivity and good sensitivity. InCYS can track Cys in situ through one-photon and two-photon fluorescence imaging in vivo . By using InCYS, we successfully revealed decreases in the levels of mitochondrial Cys in the ankles of mice with arthritis. This work provides an effective bio-target for the development of novel therapeutic approaches for arthritis, and a practical tool for revealing the connections between mitochondrial oxidative stress and severe diseases.
研究领域:
生物医学工程
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免疫学
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药理学
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
INFLAMMATION
·
2024
·
IF 4.5
Liu Qi,Zhao Yubo,Dong Shuai,Bai Xingyuan,Chen Bin,Liu Xijuan,Shen Jing,Zhu Dan
Deciphering the complex and redundant process of acute inflammation remains challenging. The failure of numerous clinical trials assessing anti-inflammation agents which had promising preclinical effects inevitably questions the validity of current animal models of inflammation. This study aimed to better understand the process of immune inflammatory response and to select more suitable models to evaluate the effect of potential anti-inflammatory drugs. Zymosan and λ-carrageenan are the most used representatives of particulate and soluble irritants that trigger acute inflammation in the air pouch inflammation model. When zymosan was used, the number of exudate cells first increased at 4 h-8 h, followed by a drop at 12 h-24 h. While, the changes in number of leukocytes in peripheral blood and proportion of neutrophils in bone marrow have the opposite trend. Meanwhile, neutrophils released neutrophil extracellular traps (NETs) to clean zymosan particles. In contrast, the cell migration response to carrageenan increased during 4 h to 24 h, no obvious NETs were observed, and the number of leukocytes in peripheral blood increased and the proportion of neutrophils in bone marrow decreased slightly. This study indicated that although both zymosan and carrageenan are sterile irritants, the characteristics of the inflammatory response induced by each other were different. In the acute phase of inflammation, zymosan-stimulated neutrophils were mobilized, recruited, and engulfed, and then died by NETs. Carrageenan stimulated the production of cytokines/chemokines by neutrophils or macrophages, but did not lead to an obvious death by releasing NETs. Graphical Abstract
研究领域:
免疫学
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药理学
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炎症研究
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动物模型
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）