CHEMICAL ENGINEERING SCIENCE
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2026
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IF 5.1
Hongji Li,Mingqian Sun,Fariza Hanim Suhailin,Dan Zhang,Tingting Sun,Zimo Yuan,Dandan Wang,Wei Sun,Changming Li
This work presents a novel flexible imprinted membrane-made surface-enhanced Raman scattering (SERS) for selective detection of microcystins-LR (MC-LR) in water. Silver nanocubes decorated with titanium dioxide (Ag NCs@TiO 2 ) nanoparticles (NPs) are dispersed on a flexible polydimethylsiloxane (PDMS) membrane as the SERS substrate. The PDMS exhibits excellent optical properties, ensuring the detection accuracy. A photo-initiated polymerization process is employed to maintain the molecular integrity of MC-LR while improving the resistance of the SERS substrates to air-induced oxidation. By optimizing the reaction condition, optimal SERS imprinted membrane is applied to selective detection of practical samples. The characterization results indicate that the Ag NCs@TiO 2 molecular imprinted membranes (ATP-MIMs) present accurate detection property to the MC-LR molecules from complex solution. Rapid detection within around 1 min is achieved by the as-prepared flexible molecularly imprinted SERS sensor. The limit of detection of MC-LR is 0.0313 μg·L −1 and the ATP-MIMs exhibit a high stability of more than 86.6 % after 28 days. It clearly exhibits from results that ATP-MIMs hold significant potential applications to trace MC-LR detection and provide a novel approach to the water quality monitoring.
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WATER AIR AND SOIL POLLUTION
·
2026
·Vol. 237
·期 9
·553
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IF 3.5
Wang Bingyuan,Wang Fengwei,Lu Hongsheng
Microbial degradation has attracted more and more attention to be an effective way to effectively treat pesticide in the environment and save treatment costs. However, the specific degradation mechanism and the influence of microorganisms on the environment of some pesticides are still unclear. In this study, the Serratia marcescens BCD1 (PV133529) which isolated from the blueberry soil showed a 20 days degradation rate of 93.14% and 91.09% for 50 mg/L and 250 mg/L λ-cyhalothrin, respectively. Product analysis revealed the pathway of λ-cyhalothrin degradation by Serratia marcescens BCD1, including the removal of cyano groups and the cleavage of ester bonds. The morphological changes of BCD1 in the presence of different concentrations of λ-cyhalothrin were observed by SEM electron microscopy, and the cell wall of BCD1 was wrinkled and deformed, and the cell length was shortened under the stress of λ-cyhalothrin. This study indicates that BCD1 as a promising microorganism for λ-cyhalothrin residue and improve blueberry quality in a treatment setting. Graphical
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NEOTROPICAL ENTOMOLOGY
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2026
·Vol. 55
·期 1
·9
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IF 1.8
Zheng Kang-Wu,Wei Zi-Han,Xia Zi-Nan,Wang Bo-Kun,Gao Ping,Li Yu-Ting,Yang Xue-Qing
Rising ambient temperatures driven by global warming are subjecting insect populations to thermal conditions exceeding their historical environmental ranges. Understanding how temperature alters key life-history traits is essential for optimizing integrated pest management strategies, particularly for invasive species. This study investigated the effects of short-term thermal stress (31 °C for the parental generation, with offspring returned to 26℃) on life history traits and insecticide sensitivity in the codling moth Cydia pomonella (L.), a major agricultural invasive pest. Results demonstrated that elevated temperature significantly shortened larval developmental duration and reduced pupal weight and overall fitness, with these phenotypic effects persisting across generations. Additionally, increased temperature attenuated the sensitivity of C. pomonella to lambda -cyhalothrin, especially in parental generation. In contrast, reproductive output and sensitivity to chlorantraniliprole remained largely unaffected by thermal elevation. Activities of detoxification enzymes including cytochrome P450 monooxygenases (P450s), carboxylesterases (CarEs), and the antioxidant enzyme catalase (CAT), were significantly upregulated at elevated temperatures, with transgenerational inheritance observed in progeny, potentially contributing to the observed decrease in insecticide sensitivity. These findings advance our understanding of the transgenerational physiological and biochemical adaptions of C. pomonella to thermal stress, with implications for resistance management under climate change scenarios.
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JOURNAL OF CHROMATOGRAPHY A
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2026
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IF 4
Huiru Xu,Yuqing Zong,Kehan Yin,Rimao Hua,Xiangwei Wu,Jiaying Xue
Water resources are increasingly contaminated by industrial and agricultural pollutants, with pesticide residues posing a persistent challenge. Most existing methods were designed for a single purpose of either measuring or eliminating water pollutants, while integrated strategies containing both functions remain scarce. Here we reported a novel semi-automatic silicone rubber (SR) based multi-vial microextraction technique for the simultaneous determination and removal of fifteen pesticides from aqueous matrices. An integrated adsorption-desorption platform enabled effective in-situ extraction of the target pesticides via hydrophobic effects. Key parameters including ultrasonication extraction for 45 min and two sequential 5-min desorption with 5 mL of hexane/ethyl acetate (1:1, v/v) were employed. The quantitative analysis was validated in real waters of the Nanfei River, Chao Lake, and tap water with good recoveries (72.8–112.6 %) and relative standard deviations (0.1–7.5 %), superior limits of detection (0.0020–0.20 μg/L) and quantification (0.006 to 0.50 µg/L), and free matrix effects (87.5–102.1 %) by gas chromatography equipped with electron capture detector. Focusing on its selectivity and recyclability, the proposed approach was further applied to purifying green tea and chrysanthmum tea infusions, achieving over 90 % removal efficiency for most pesticides. These results demonstrated that the semi-automatic SR-based multi-vial microextraction is a robust and effective method for both quantifying and eliminating target pesticides from waters and tea infusions. The high throughput and low cost encourage its potential for customized commercial application in monitoring and remediating emerging pesticides in environment and food fields.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2026
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IF 4.8
Yu-Ze Li,Chao Hu,Yu-Xi Liu,Yun-Tong Lv,Xue-Qing Yang
Extensive studies have demonstrated that cytochrome P450 enzymes play a critical role in the metabolic detoxification and resistance development of pests against insecticides; however, relative to P450 genes in Clan 3, knowledge on mitochondrial Clan members remains limited. Here, we report the involvement of a CYP333 family member, CYP333B119v2 , in conferring lambda -cyhalothrin resistance in Cydia pomonella . Phylogenetic analysis shows that CYP333B119v2 clusters with CYP333 family members of Lepidoptera, it containing three highly conserved P450 domains: the Heme-binding region, C-Helix, and K-helix. Spatiotemporal expression profiling indicates that CYP333B119v2 transcription levels increase progressively during larval development, peaking at the fourth instar, with significant expression in the midgut, the primary detoxification tissue. Relative to susceptible population (SS), CYP333B119v2 expression is 2.73-fold higher in lambda -cyhalothrin moderate resistant population (LCR) and is significantly induced by sublethal doses of lambda -cyhalothrin . Knockdown of CYP333B119v2 enhances larval susceptibility to lambda -cyhalothrin. Molecular docking based on AlphaFold models revealed strong binding interactions between CYP333B119v2 and lambda -cyhalothrin, with diverse non-covalent interactions involved in the binding process. These findings suggest that CYP333B119v2 may contribute to lambda -cyhalothrin resistance in C. pomonella . This study is the first report implicating a mitochondrial CYP333B subfamily member in the resistance mechanisms of Lepidoptera, advancing our understanding of P450-mediated metabolic resistance and providing new insights into pest management strategies.
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NEW JOURNAL OF CHEMISTRY
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2026
·
IF 2.5
Weina Zhang,Hongji Li,Yuming Liu,Mengyang Xu,Dandan Wang
In this study, array structure SERS substrate was designed by using the polydimethylsiloxane (PDMS) as the support membrane and loaded Au@Ag@Au nanoparticles on its surface. By the three-phase self-assembly procedure, the Au@Ag@Au nanoparticles could smoothly transferred on the PDMS membrane and presented array structure, which could significantly promote the SERS sensitivity. Furthermore, molecularly imprinted polymers (MIPs) were coated on the surface of membrane to promote the selective detection property. In order to protect the chemical structure of MC-LR molecules, the Au@Ag@Au/PDMS molecularly imprinted membranes (AAAP-MIMs) were synthesized by the photo-initiated polymerization. It was demonstrated from the results that the AAAP-MIMs exhibited high SERS sensitivity, stability, and uniformity. This investigation presented a novel analysis procedure applied into the toxic microcystin selective detection.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 2
·97
·
IF 5.3
Liu Bo,Han Qianxi,Wang Wenxuan,He Lijing,Wang Wenjie,Cheng Yanfen,Yun Shaojun,Cheng Feier,Feng Cuiping,Cao Jinling
Pyrethroid pesticide residues pose a significant global public health challenge, particularly in complex edible fungus matrices where trace, structurally similar pesticides are difficult to distinguish and detect. To address this critical gap, a novel surface-enhanced Raman spectroscopy (SERS) platform is presented integrating “magnetic enrichment, covalent organic framework (COF)-mediated capture, silver shell enhancement, and machine learning (ML)-driven intelligent recognition”. The core innovation lies in the tailored Fe₃O₄@COF@Ag nanocomposite architecture where the magnetic Fe₃O₄ core enables rapid sample separation, the COF intermediate layer provides specific molecular capture, and the Ag shell generates dense SERS “hotspots”, synergistically enhanced by ML algorithms to enable precise discrimination of structurally analogous pesticides. The developed Fe₃O₄@COF@Ag substrate demonstrated exceptional performance, achieving detection limits as low as 5.21 µg/kg for deltamethrin (CF), 6.08 µg/kg for fenvalerate (FV), and 11.6 µg/kg for lambda-cyhalothrin (LCF). The method exhibited outstanding linearity (R² > 0.99) for quantitative analysis and anti-interference capability, with recoveries of 94.36-106.89% in real samples. By integrating ML algorithms, the platform achieved 100% classification accuracy for distinguishing structurally similar pyrethroids. Principal component analysis (PCA) and support vector machine (SVM) models effectively extracted discriminative spectral features, enabling precise identification even at trace concentrations. This work provides a rapid, sensitive, and field-deployable solution for pesticide monitoring, offering significant potential to enhance food safety and public health protection. Graphical
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PEST MANAGEMENT SCIENCE
·
2023
·
IF 4.1
Yifan Li,Shujun Ni,Yunping Wang,Ruichi Li,Hong Sun,Xuan Ye,Zhen Tian,Yalin Zhang,Jiyuan Liu
BACKGROUND Insecticide resistance continuously poses a threat to agricultural production. Chemosensory protein-mediated resistance is a new mechanism of insecticide resistance discovered in recent years. In-depth research on resistance mediated by chemosensory proteins (CSPs) provides new insight into aid insecticide resistance management. RESULTS Chemosensory protein 1 in Plutella xylostella ( PxCSP1 ) was overexpressed in the two indoxacarb-resistant field populations and PxCSP1 has a high affinity with indoxacarb. PxCSP1 was upregulated when exposed to indoxacarb and the knockdown of this gene elevated sensitivity to indoxacarb, which demonstrate that PxCSP1 is involved in the indoxacarb resistance. Considering that CSPs may confer resistance in insects via binding or sequestering, we explored the binding mechanism of indoxacarb in PxCSP1 -mediated resistance. Using molecular dynamics simulations and site-directed mutation, we found that indoxacarb forms a solid complex with PxCSP1 mainly through van der Waals interactions and electrostatic interactions. Between these, the electrostatic interaction provided by the Lys100 side chain in PxCSP1 , and especially the hydrogen bonding between the NZ atom and the O of the carbamoyl carbonyl group of indoxacarb, are the key factors for the high affinity of PxCSP1 to indoxacarb. CONCLUSIONS The overexpression of PxCPS1 and its high affinity to indoxacarb is partially responsible for indoxacarb resistance in P. xylostella . Modification of indoxacarb's carbamoyl group has the potential to alleviate indoxacarb resistance in P. xylostella . These findings will contribute to solving chemosensory protein-mediated indoxacarb resistance and provide a better understanding of the insecticide resistance mechanism. © 2023 Society of Chemical Industry.
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JOURNAL OF HAZARDOUS MATERIALS
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2023
·
IF 13.6
Yan Liu,Shukun Tang,Xu Wang,Xiaoliang Wang,Xianghua Tang,Qian Wu,Zunxi Huang,Junmei Ding
The long-term and excessive use of pyrethroid pesticides poses substantial health risks and ecosystem concerns. Several bacteria and fungi have been reported that could degrade pyrethroids . The ester-bond hydrolysis using hydrolases is the initial regulatory metabolic reaction of pyrethroids. However, the thoroughly biochemical characterization of hydrolases involved in this process is limited. Here, a novel carboxylesterase, designated as EstGS1 that could hydrolyze pyrethroid pesticides was characterized. EstGS1 showed low sequence identity (<27.03%) compared to other reported pyrethroid hydrolases and belonged to the hydroxynitrile lyase family that preferred short short-chain acyl esters (C2 to C8). EstGS1 displayed the maximal activity of 213.38 U/mg at 60 °C and pH 8.5 using p NPC2 as substrate, with K m and V max were 2.21 ± 0.72 mM and 212.90 ± 41.78 µM/min, respectively. EstGS1 is a halotolerant esterase and remains stable in 5.1 M NaCl. Based on molecular docking and mutational analysis, the catalytic triad of S 74 -D 181 -H 212 and three other substrate-binding residues I 108 , S 159 , and G 75 are critical for the enzymatic activity of EstGS1. Additionally, 61 and 40 mg/L of deltamethrin and λ-cyhalothrin were hydrolyzed by 20 U of EstGS1 in 4 h. This work presents the first report on a pyrethroid pesticide hydrolase characterized from a halophilic actinobacteria.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Zhenyu Li,Mengling Chen,Wenjie Bai,Shuxia Zhang,Liwei Meng,Wei Dou,Jinjun Wang,Guorui Yuan
Carboxylesterases (CarEs) are a multifunctional superfamily of enzymes and play an important role in detoxification of various insecticides in insects. The oriental fruit fly, Bactrocera dorsalis , is one of the most destructive agricultural pests and has developed different degrees of resistance to organophosphates in field. However, the involvement of BdCarEs in tolerance or resistance to other alternative insecticides are still unclear. In the present study, 33 BdCarEs genes were identified based on the genome database of B. dorsalis . Phylogenetic analysis demonstrated that they were classified into nine clades, with abundance of α -esterases. Meanwhile, the sequence characterization and the chromosome distribution were also analyzed. The spatiotemporal expression analysis of BdCarEs genes suggested that the diversity of potential function in different physiological processes . With the exception of BdCarE21 , all BdCarEs genes responded to at least one insecticide exposure, and BdCarE20 was found to be up-regulated after exposure to all five tested insecticides individually. Eight BdCarEs genes were overexpressed in MR strain when compared to that in SS strain. Subsequently, knockdown the expression of representative BdCarEs genes significantly increased the susceptibility of the oriental fruit fly to corresponding insecticides, which indicated that the tested BdCarEs genes contributed to one or multiple insecticide detoxification. These findings provide valuable insights into the potential role in respond to tolerance or resistance to insecticides with different mode of action, and will facilitate development of efficiency management strategy for B. dorsalis .
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CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Ying Zhang,Yunhai Chen,Dianwei Zhang,Huilin Liu,Baoguo Sun
Within this study, a peptide nanodots-bridged metal–organic framework (PNMOF) has been constructed by predictably assembling highly efficient fluorescent dipeptide nanodots into the three-dimensional framework upon coordination of Zn(II) and dihydroxamate linkers. The established system was on behalf of a new class of tripartite hybrid material, i.e., metal ion, organic linker, and peptide nanodot, which integrated the chemical and structural versatility in a modular fashion. Owing to its unique open framework with tripartite construction, PNMOF exhibited a rapid and sensitive response, therefore, the target (e.g., lambda-cyhalothrin, LCY) driven into the tripartite hybrid structure stimulated a cascade amplification effect. LCY can cause significant fluorescence quenching in the PNMOF-based cascade amplification platform due to the charge transfer mechanism, which was confirmed by density functional theory calculations. PNMOF-based cascade amplification platform was employed for fluorescence imaging detection of pyrethroids in solution and solid state assisted by the smartphone. The limit of detection was as low as 0.34 μg/L and the recoveries percentage in real agricultural products was calculated as 88.26–108.17%. This work not only underlies the structural diversity of conventional metal–organic framework but also offers a useful tool in environmental protection and food safety.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
·
IF 6.1
Chao Hu,Yu-Xi Liu,Shi-Pang Zhang,Ya-Qi Wang,Ping Gao,Yu-Ting Li,Xue-Qing Yang
Aryl hydrocarbon receptor (AhR) enhances insect resistance to insecticides by regulating the detoxification network. Our previous studies have confirmed that overexpressions of cytochrome P450 monooxygenases (P450s) and glutathione S-transferases (GSTs) are involved in lambda-cyhalothrin resistance in Cydia pomonella. Here, we report that CpAhR regulates the expression of GST and P450 genes, thus conferring resistance. Expression patterns indicated that the expression of CpAhR was highly induced by lambda-cyhalothrin exposure and upregulated in a lambda-cyhalothrin-resistant population. RNA interference (RNAi) of CpAhR decreases the expression of key resistance-related genes (CpGSTe3, CpCYP9A121, and CpCYP9A122) and the activity of the GST enzyme, reducing the tolerance to lambda-cyhalothrin. Furthermore, β-naphthoflavone, a novel agonist of AhR, was first proven to be effective in increasing CpAhR expression and larval tolerance to lambda-cyhalothrin. These results demonstrate that CpAhR regulates the expression of key detoxifying genes and GST activity, resulting in the development of resistance to lambda-cyhalothrin in C. pomonella.
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