SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Changxin Huangfu,Yu Zhang,Minseok Jang,Liang Feng
Cu 2+ , Fe 2+ and free chlorine are three important analytes in drinking water. Firstly, the μPAD for single analyte was easily fabricated. Detection limits for Cu 2+ , Fe 2+ and free chlorine were 0.1 mg L −1 , 0.1 mg L −1 and 0.2 mg L −1 , respectively, less or equal to each Chinese Drinking Water Standard concentration and lower than those of most commercially available testing strips. Another μPAD with two layers was then developed to monitor and report the safety level of these three analytes in drinking water simultaneously. Moreover, the μPADs for Fe 2+ and free chlorine provided more distinguishable colors to users with naked eyes. The as-fabricated μPAD not only demonstrated excellent anti-interference capability, production reproducibility, but also exhibited good applicability for real use in different kinds of drinking water.
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD ENGINEERING
·
2023
·
IF 5.5
Ya Liu,Yueying Zhang,Feiwu Long,Jinrong Bai,Yina Huang,Hong Gao
The accumulation of lipid oxidation and biogenic amines is the primary cause of pork sausage spoilage during storage. Therefore, this study aimed to develop a colorimetric microfluidic paper analytical device (μPAD) that specifically responded to these two factors to evaluate sausage spoilage during storage. A dataset consisting of 4096 images of μPADs was collected, and a convolutional neural networks (CNN)-based classification model (Resnet50) was trained and tested with an accuracy, F1 score, and recall rate of 97.10%, 97.14%, and 99.17%, respectively. Additionally, the CNN-based model was integrated into a smartphone application with user-friendly interfaces catering to both professional and non-professional users. Furthermore, the classification method was validated by predicting the shelf life of sausages stored at different temperatures. Compared to conventional methods, this approach provided a cost-effective, rapid, and portable detection method for assessing pork sausage freshness while broadening the application of smartphone-based colorimetric μPADs in food safety and quality control.
研究领域:
食品科学
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人工智能
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Haorui Cao,Kang Mao,Jiajia Yang,Qingqing Wu,Jiming Hu,Hua Zhang
The arsM gene is a critical biomarker for the potential risk of arsenic exposure in paddy soil. However, on-site screening of arsM is limited by the lack of high-throughput point-of-use (POU) methods. Here, a multiplex CRISPR/Cas12a microfluidic paper-based analytical device (μPAD) was constructed for the high-throughput POU analysis of arsM, with cascade amplification driven by coupling crRNA-enhanced Cas12a and horseradish peroxidase (HRP)-modified probes. First, seven crRNAs were designed to recognize arsM, and their LODs and background signal intensities were evaluated. Next, a step-by-step iterative approach was utilized to develop and optimize coupling systems, which improved the sensitivity 32 times and eliminated background signal interference. Then, ssDNA reporters modified with HRP were introduced to further lower the LOD to 16 fM, and the assay results were visible to the naked eye. A multiplex channel microfluidic paper-based chip was developed for the reaction integration and simultaneous detection of 32 samples and generated a recovery rate between 87.70 and 114.05%, simplifying the pretreatment procedures and achieving high-throughput POU analysis. Finally, arsM in Wanshan paddy soil was screened on site, and the arsM abundance ranged from 1.05 × 106 to 6.49 × 107 copies/g; this result was not affected by the environmental indicators detected in the study. Thus, a coupling crRNA-based cascade amplification method for analyzing arsM was constructed, and a microfluidic device was developed that contains many more channels than previous paper chips, greatly improving the analytical performance in paddy soil samples and providing a promising tool for the on-site screening of arsM at large scales.
研究领域:
环境科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 36
·5795-5801
·
IF 4.776
Xuemei Yin,Linlin Liang,Peini Zhao,Feifei Lan,Lina Zhang,Shenguang Ge,Jinghua Yu
MicroRNAs (miRNAs) are a class of significant biomarkers; however, it is still a huge challenge to express them accurately. Herein, a fluorescent/colorimetric dual-model biosensor based upon the quenching effect of graphitic carbon nitride on palladium nanoclusters (Pd NCs) on the platform of a microfluidic paper-based analytical device was built for the detection of miRNAs. On the one hand, Pd NCs could catalyze a chromogenic reaction so that preliminary detection was achieved by the naked eye. On the other hand, the fluorescence analysis combined with nucleic acid cycle signal amplification was required to get precise result and the detection limit is 3 fM, which was superior to the previous method. What's more, this biosensor could be designed to detect other miRNAs via changing the corresponding aptamer sequences. Therefore, the as-constructed biosensor supplies a versatile platform to conduct point-of-care detection of miRNAs with outstanding performance.
研究领域:
生物化学
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分子诊断
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纳米技术
·
医学检测
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2016
·Vol. 141
·期 19
·5511-5519
·
IF 4.033
Miao Chen,Hua Yang,Liya Rong,Xiaoqing Chen
A novel facile method for on-site determination of sulphite in wine using a gas-diffusion microfluidic paper-based analytical device (μPAD) combined with surface-enhanced Raman spectroscopy (SERS) was explored. The inexpensive and disposable μPAD was realized by sandwiching the ZnO-paper disc and integrating gas-diffusion separation and gas preconcentration on a paper-based platform. Through extensive condition optimization, the SERS signal at a shift of 620 cm−1 and the concentration of SO2 showed good linearity in the range of 5–300 μg mL−1. The linear correlation coefficient was 0.995 and the detection limit for sulphite was found to be 2 μg mL−1. Ultimately, this proposed method was applied to the determination of sulphite in wine, which can be essentially paralleled with the official method. Endowed with portability, minimal reagent consumption, and operational simplicity, this developed device would find broad utilization for on-site monitoring of sulphite.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
WanYun Li,ShiYu Lu,ShuJuan Bao,ZhuanZhuan Shi,Zhisong Lu,ChangMing Li,Ling Yu
A visual colorimetric microfluidic paper-based analytical device (μPAD) was constructed following the direct synthesis of enzyme-inorganic hybrid nanomaterials on the paper matrix. An inorganic solution of MnSO 4 and KH 2 PO 4 containing a diluted enzyme (glucose oxidase, GOx) was subsequently pipetted onto cellulose paper for the in situ growth of GOx@Mn 3 (PO 4 ) 2 hybrid functional materials. The characterization of the morphology and chemical composition validated the presence of hybrid materials roots in the paper fiber, while the Mn 3 (PO 4 ) 2 of the hybrid provided both a surface for enzyme anchoring and a higher peroxidase-like catalytic activity as compared to the Mn 3 (PO 4 ) 2 crystal that was synthesized without enzyme modulation. This new approach for the in situ growth of an enzyme-inorganic hybrid on a paper matrix eliminates centrifugation and the dry process by casting the solution on paper. The sensing material loading was highly reproducible because of the accuracy and stability of pipetting, which eventually contributed to the reliability of the μPAD. The self-assembled natural and artificial enzyme hybrid on the μPADs specifically detected glucose from a group of interferences, which shows great specificity using this method. Moreover, the colorimetric signal exhibited detection limitation for glucose is 0.01 mM, which lies in the physiological range of glucose in biological samples.
研究领域:
分析化学
·
生物传感器
·
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Yunlong Nie,Fang Zhou,Chenye Wang
A simple paper-based microfluidic device was fabricated to simultaneously detect multiple targets. Microfluidic paper-based analytical devices (μPAD) comprise a single-layer moving sliding PAD (SPAD) to control the flow channel switch together with a folding origami PAD (OPAD) to test the target analytes. The facile assembly without any splicing materials avoids cross-contamination and non-specific adsorption of joining materials that may be caused by multi-target detection. The concentration of Fe(III), Ni(II), Cr(VI), and nitrite in standard solutions and actual aqueous solutions was successfully determined using the designed μPAD. The μPAD was able to achieve LOD of 3.3 mg/L, 1.3 mg/L, 0.35 mg/L, 0.28 mg/L for Fe (III), Ni (II), Cr (VI), and nitrite, respectively. The designed SOPAD exhibits improved stability, with a deviation of less than 7% compared to conventional analytical methods (ICP-OES and UV). Our work demonstrates that this 3D PAD holds great promise and a wide scope in environmental monitoring, biochemical analysis, food testing and other testing industries.
研究领域:
微流控技术
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分析化学
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环境科学
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生物化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 10
·818
·
IF 5.743
Jiali Huang,Xu Li,Mingzhen Xiu,Kang Huang,Kang Cui,Jing Zhang,Shenguang Ge,Shiji Hao,Jinghua Yu,Yizhong Huang
Nowadays, developing a cost-effective, easy-to-operate, and efficient signal amplification platform is of important to microfluidic paper-based analytical devices (μPAD) for end-use markets of point-of-care (POC) assay applications. Herein, an ultrasensitive, paper-based photoelectrochemical (PEC) bioassay platform is constructed by in situ grown ZnO/ZnIn2S4heterojunctions onto paper fibers, which acted as photoactive signal amplification probes for enhancing the sensitivity of antibodies-based diagnostic assays, for the sensitive detection of alpha-fetoprotein (AFP) targets. The crystalline flake-like ZnIn2S4composited with hexagonal nanorods (NRs) morphology of ZnO is an in situ grown, at the first time, onto cellulose fibers surface supported with Au nanoparticle (Au NP) modification to improve conductivity of the device working zone. The obtained composites on paper fibers are implemented as a flexible paper-based photoelectrode to realize remarkable performance of the fabricatedμPAD, resulting from the enhanced PEC activity of heterojunctions with effective electron-hole pair separation for accelerating photoelectric conversion efficiency of the sensing process under light irradiation. Once the target AFP was introduced into the biosensing interface assistant, with a specific recognition interaction of AFP antibody, a drastically photocurrent response was generated, in view of the apparent steric effects. With the concentration increase of AFP targets, more immune conjugates could be confined onto the biosensing interface, eventually leading to the quantitative decrease of photocurrent intensity. Combined with an ingenious origami design and permitting the hydrophobic/hydrophilic conversion procedure in the bioassay process, the ultrasensitive PEC detection of AFP targets was realized. Under the optimized conditions, the level of AFP could be sensitively tracked by the preparedμPAD with a liner range from 0.1 to 100 ng mL−1and limit of detection of 0.03 ng mL−1. This work provides a great potential application for highly selective and sensitive POC testing of AFP, and finally, developments for clinical disease diagnosis.Keywords:μPAD;photoelectrochemical;alpha-fetoprotein;point-of-care assay;immunosensing
研究领域:
生物医学工程
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分析化学
·
即时诊断
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）