FOOD CHEMISTRY
·
2024
·
IF 8.5
Fuyuan Huang,Yayun Jiang,Qiaoli Wu,Chaochuan Zheng,Shen Huang,Huan Yang,Guangxin Xiang,Laibao Zheng
Loop-mediated isothermal amplification (LAMP) is a rapid and sensitive nucleic acid testing method for pathogen detection, yet the absence of a straightforward readout strategy remains challenging. We've successfully designed polyethyleneimine-stabilized gold nanoclusters (PEI-AuNCs) as a cationic AuNCs indicator tailored for distinguishing LAMP results, enabling direct visual inspection under UV light. Positive LAMP reactions with PEI-AuNCs, in combination with magnesium pyrophosphate crystals, yield red-fluorescent bulk precipitates visible to the naked eye. To address contamination concerns, we introduced a one-pot reaction by incorporating AuNCs into the lid recess. This one-pot LAMP assay demonstrates exceptional detection capability, identifying Salmonella enterica at concentrations as low as 10 1 CFU/mL within approximately 50 min, excluding nucleic acid extraction. The platform's versatility, achieved through customizable primers, positions it as a promising molecular diagnostic tool for rapid and visual pathogen detection across scientific disciplines.
研究领域:
分子诊断
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纳米技术
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病原体检测
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Qing Han,Xinlan Bian,Yue Chen,Bingzhi Li
Flap endonuclease I (FEN1) is a structure-specific nuclease that participates in DNA repair, and its overexpression is related to an increased risk of cancer. Currently, few FEN1 assays can meet the requirements for diagnostic purposes due to their high complexity, low specificity, and limited sensitivity. Herein, we developed a biosensor adopting synthetic biology strategy, called CLAIM (cleavage-ligation-activation-involved monitoring) for FEN1 quantification. CLAIMintegrates ligation-based T7 promoter repair, RNA generation, and CRISPR/Cas13a. FEN1 cleavages the DNA probe with a 5′-flap to generate a nick site that can be ligated to form a T7 promoter. Subsequent transcription produces abundant RNA that can activate Cas13a, generating a fluorescence signal due to the trans -cleavage of Cas13a to a fluorogenic reporter. CLAIM was able to detect FEN1 with low femtomolar sensitivity, and its accuracy was validated using samples of cancer and non-tumorigenic cell lines. Interestingly, we found that the utilization of thermostable T4 DNA ligase is critical to make the cascade of FEN1 cleavage, ligation, and transcription take place in one-pot. CLAIM was also repurposed for validating FEN1 inhibitors, indicating its potential in drug screening. This work provides a diagnostic tool for FEN1 analysis and opens up opportunities for FEN1 biosensing and related drug discovery.
研究领域:
分子生物学
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合成生物学
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癌症诊断
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生物技术
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Ting Xie,Li Zhou.,Laichuang Han,Cuiping You,Zhongmei Liu,Wenjing Cui,Zhongyi Cheng,Junling Guo,Zhemin Zhou
Pullulanase is a starch-debranching enzyme that hydrolyzes side chain of starch, oligosaccharides and pullulan. Nevertheless, the limited activities of pullulanases constrain their practical application. Herein, the hyperthermophilic type II pullulanase from Pyrococcus yayanosii CH1 (Pul PY2 ) was evolved by synergistically engineering the substrate-binding pocket and active-site lids. The resulting mutant Pul PY2 -M2 exhibited 5-fold improvement in catalytic efficiency ( k cat / K m ) compared to that of Pul PY2 . Pul PY2 -M2 was utilized to develop a one-pot reaction system for efficient production of maltooligosaccharides. The maltooligosaccharides conversion rate of Pul PY2 -M2 reached 96.1%, which was increased by 5.4% compared to that of Pul PY2 . Furthermore, when employed for glucose production, the glucose productivity of Pul PY2 -M2 was 25.4% and 43.5% higher than that of Pul PY2 and the traditional method, respectively. These significant improvements in maltooligosaccharides and glucose production and the efficient utilization of corn starch demonstrated the potential of the engineered Pul PY2 -M2 in starch sugar industry.
研究领域:
生物化学
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生物技术
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酶学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2023
·
IF 4.2
Rongrong Li,Wenqi Liu,Huibin Liu,Hongyu Niu,Quan Zhou,Changhai Liang,Xiao Chen
One-pot hydrogenation of biomass-derived furfural with multiple reduction sites requires suitable metal sites to achieve a high value-added chemical in high yield. In this work, nonprecious Ni–Fe bimetallic catalysts with high efficiency derived from FexNiy/MgAl-LDH hydrotalcite precursors are prepared for one-pot sequential hydrogenation of furfural to tetrahydrofurfuryl alcohol at mild conditions. Through fine adjustment of metallic Fe/Ni molar ratios in hydrotalcite precursors, bimetallic FexNiy/MgAlO catalysts can be constructed. The topological transformation of hydrotalcite precursors provides a mesoporous structure for bimetallic supported catalysts. Due to the formation of the Ni–Fe alloy, there is an electron transfer from Fe to Ni in the catalyst due to the higher electronegativity of Ni than that of Fe, which greatly influences the adsorption of furfural on the surface of catalysts. Comparing the structures and catalytic performances of monometallic Ni/MgAlO and Fe/MgAlO catalysts, the Ni-rich Fe1Ni4/MgAlO catalyst with the dual active sites of metallic Ni and FeNi3 alloy presents high efficiency in sequential hydrogenation of the C═O bonds and C═C bonds of furfural to tetrahydrofurfuryl alcohol with the yield of 90.3% under 3 MPa H2 and 80 °C for 3 h, which can be an alternative for noble metal catalysts. Additionally, the Ni-rich Fe1Ni4/MgAlO catalyst presents excellent cycling stability. This design strategy for nonprecious NiFe bimetallic catalysts not only provide promising candidates for effective utilization of biobased furfural but also extend the application of bimetallic catalysts derived from hydrotalcite precursors for one-pot hydrogenation reactions.
研究领域:
催化
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化学工程
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材料科学
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绿色化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Thi Le Cham-Bich,Nguyen Trinh Hao,Thi Le Mai Ngoc,Liem The Ho,Phuong Hoang Tran
The selective oxidation of biomass-derived carbohydrates into high-value platform chemicals represents a promising avenue for sustainable chemical production. In our work, we explored the catalytic performance of a bimetallic Fe/Cu loaded in carbon decorated with nitrogen-sulfur (Fe/Cu@NSC) in the conversion of fructose/glucose into 2,5-diformylfuran (DFF). The catalyst exhibited remarkable efficiency, particularly with fructose, achieving a peak DFF yield of 61.1% under optimized conditions. In contrast, glucose conversion proved less effective, with a maximum DFF yield of 29.1% at 160 °C for 15 h. Methodical examinations of reaction parameters, such as solvent composition, and reaction time, yielded crucial insights into the determinants of product dispersion and catalytic efficacy. Moreover, reusability evaluations demonstrated a slow reduction in catalytic activity throughout repeated cycles; yet, this negligible fall still affirms the promise of catalysts in green chemistry. In addition, the catalyst structure was evidenced by FTIR, XPS, HRTEM, SEM, BET, ICP-MS, NH 3 -TPD, EDX, elemental mapping analyses, and the thermal stability of the catalyst was evaluated by TGA. These findings underscore the potential of Fe/Cu@NSC as an effective and recyclable catalyst for DFF synthesis while highlighting the necessity for further optimization to enhance its long-term stability and performance.
研究领域:
催化化学
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绿色化学
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生物质利用
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可持续化学品生产
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
ChemCatChem
·
2024
·
IF 3.8
Cong Luo,Haoru Tang,Houfang Lu,Kejing Wu,Yingying Liu,Yingming Zhu,Binshen Wang,Bin Liang
Graphical A high glycerol carbonate yield of 90 % is achieved by the one–pot reaction of CO 2 , propylene oxide, and glycerol catalysed by DBU–based PIL, which can be facilely recovered and steadily reused without deactivation. The development of efficient heterogeneous catalysts for upgrading CO 2 , a greenhouse gas, and glycerol (Gly), an untapped biomass resource, into glycerol carbonate (GC) remains a challenge. Herein, 1,8-diazabicyclo[5.4.0] undec–7–ene (DBU)–based poly (ionic liquid)s (PILs) were constructed for the one–pot synthesis of GC from CO 2 , Gly, and propylene oxide. Among them, P−DVB−DBUVBI–1 exhibited the best catalytic performance, achieving a GC yield of 90 % under optimal reaction conditions (100 °C, 6 h). The PIL catalyst was easily recovered and reused, as demonstrated by stable activity over five cycles. Density functional theory calculations showed that owing the strong positive charge delocalization ability of DBU onium, DBU–based PILs exhibit high nucleophilicity, which is the key to their efficient catalytic performance in the one–pot reaction.
研究领域:
化学
·
材料科学
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环境科学
·
催化
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2019
·Vol. 10
·期 1
·1-11
·
IF 11.878
Zong Yu,Fang Fang,Meyer Kirsten J.,Wang Liguo,Ni Zhihao,Gao Hongying,Lewis Kim,Zhang Jingren,Rao Yu
Teixobactin represents a new class of antibiotics with novel structure and excellent activity against Gram-positive pathogens and Mycobacterium tuberculosis . Herein, we report a one-pot reaction to conveniently construct the key building block l -allo-Enduracidine in 30-gram scale in just one hour and a convergent strategy (3 + 2 + 6) to accomplish a gram-scale total synthesis of teixobactin. Several analogs are described, with 20 and 26 identified as the most efficacious analogs with 3~8-fold and 2~4-fold greater potency against vancomycin resistant Enterococcus faecalis and methicillin-resistant Staphylococcus aureus respectively in comparison with teixobactin. In addition, they show high efficiency in Streptococcus pneumoniae septicemia mouse model and neutropenic mouse thigh infection model using methicillin-resistant Staphylococcus aureus . We also propose that the antiparallel β-sheet of teixobactin is important for its bioactivity and an antiparallel dimer of teixobactin is the minimal binding unit for lipid II via key amino acids variations and molecular docking.
研究领域:
药物化学
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微生物学
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有机合成
·
分子生物学
✓ 已验证
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Cong Zheng,Pan Wei,Wei Dai,Linlin Wang,Botao Song,Pengxiang Jia,Yongkuan Gong
In this paper, a series of random copolymers poly(methacrylic acid - co -2-methacryloyloxyethyl phosphorylcholine) P(MAA- co -MPC) were synthesized firstly via RAFT living polymerization. The P(MAA- co -MPC) copolymer side chains bear cell membrane phosphorylcholine zwitterions to endow biocompatibility and carboxylic groups to confer coordination with metal ions. Thus, the copolymer was adopted to modify Fe 3 O 4 nanoparticle by a one-pot coprecipitation approach. The effects of the copolymer composition as well as the ratio between the copolymers and iron ions on the performances of the magnetite nanoparticles were researched. The diameters of the nanoparticles could be easily tuned by changing the initial copolymer amount. Moreover, a long-term colloidal stability of magnetite particles was obtained after P(MAA- co -MPC) modification. Biocompatibility of the P(MAA- co -MPC) copolymer coated magnetite nanoparticles was investigated by protein adsorption, in vitro cytotoxicity and cell uptake studies. It was found that the copolymer content of magnetite nanoparticles correlates with its biocompatibility. Excellent biocompatibility could be obtained when the content of the copolymer in the composite nanoparticles reached to 54%.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
·
高分子化学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xiaoxiao Liu,Kai Li,Luona Ye,Xinghong Cao,Pengbo Wang,Xiaoman Xie,Min Yang,Li Xu,Yunjun Yan,Jinyong Yan
We propose a co-immobilized chemo-enzyme cascade system to mitigate random intermediate diffusion from the mixture of individual immobilized catalysts and achieve a one-pot reaction of multi-enzyme and reductant. Catalyzed by lipase and lipoxygenase, unsaturated lipid hydroperoxides (HPOs) were synthesized. 13( S )-hydroperoxy-9Z, 11E-octadecadienoic acid (13-HPODE), one compound of HPOs, was subsequently reduced to 13( S )-hydroxy-9Z, 11E-octadecadienoic acid (13-HODE) by cysteine. Upon the optimized conditions, 75.28 mg of 13-HPODE and 4.01 mg of 13-HODE were produced from per milliliter of oil. The co-immobilized catalysts exhibited improved yield compared to the mixture of individually immobilized catalysts. Moreover, it demonstrated satisfactory durability and recyclability, maintaining a relative HPOs yield of 78.5% after 5 cycles. This work has achieved the co-immobilization of lipase, lipoxygenase and the reductant cysteine for the first time, successfully applying it to the conversion of soybean oil into 13-HODE. It offers a technological platform for transforming various oils into high-value products.
研究领域:
生物催化
·
酶工程
·
有机化学
·
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-14
·
IF 14.7
Wei Xinlei,Yang Xue,Hu Congcong,Li Qiangzi,Liu Qianqian,Wu Yue,Xie Leipeng,Ning Xiao,Li Fei,Cai Tao,Zhu Zhiguang,Zhang Yi-Heng P. Job,Zhang Yanfei,Chen Xuejun,You Chun
In vitro biotransformation (ivBT) facilitated by in vitro synthetic enzymatic biosystems (ivSEBs) has emerged as a highly promising biosynthetic platform. Several ivSEBs have been constructed to produce poly-3-hydroxybutyrate (PHB) via acetyl-coenzyme A (acetyl-CoA). However, some systems are hindered by their reliance on costly ATP, limiting their practicality. This study presents the design of an ATP-free ivSEB for one-pot PHB biosynthesis via acetyl-CoA utilizing starch-derived maltodextrin as the sole substrate. Stoichiometric analysis indicates this ivSEB can self-maintain NADP + /NADPH balance and achieve a theoretical molar yield of 133.3%. Leveraging simple one-pot reactions, our ivSEBs achieved a near-theoretical molar yield of 125.5%, the highest PHB titer (208.3 mM, approximately 17.9 g/L) and the fastest PHB production rate (9.4 mM/h, approximately 0.8 g/L/h) among all the reported ivSEBs to date, and demonstrated easy scalability. This study unveils the promising potential of ivBT for the industrial-scale production of PHB and other acetyl-CoA-derived chemicals from starch.
研究领域:
生物技术
·
生物化学
·
合成生物学
·
代谢工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）