CHEMOSPHERE
·
2023
·
IF 8.8
Shuheng Hu,Xin Jin,Xueyan Chen,Bin Lv,Wenhao Xi,Yan Lan,Wei Han,Zimu Xu,Cheng Cheng
Over the past few decades, the frequent and excessive usage of pesticides has had detrimental effects on the soil and other habitats. In terms of removing organic contaminants from soil, non-thermal plasma has become one of the most competitive advanced oxidation methods. The study used dielectric barrier discharge (DBD) plasma to repair soil contaminated by butachlor (BTR). BTR degradation was investigated in actual soil under various experimental parameters. According to the results, DBD plasma treatment at 34.8 W destroyed 96.10% of BTR within 50 min, and this degradation was consistent with the model of first order kinetics. Boosting the discharge power, lowering the initial BTR concentration, using appropriate soil moisture content and air flow rate, and using oxygen as the working gas for discharge are all beneficial to the degradation of BTR. The changes in soil dissolved organic matter (DOM) before and after plasma treatment were assessed using a total organic carbon (TOC) analyzer. A Fourier transform infrared (FTIR) spectroscopy and an Ultra Performance Liquid Chromatography Tandem Mass Spectrometry (UPLC-MS) were employed to investigate the degradation of BTR. A wheat growth test showed that the best growth was achieved at 20 min of plasma soil remediation , but too long treatment would lower soil pH and thus affect wheat growth.
研究领域:
环境科学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Yi Wu,Liyuan Zhang,Dongjie Zhang,Runzhong Yu
A microfluidic paper chip colorimetric detection system based on surface molecular imprinting of zinc ferrite nanoparticles was established, and the detection images were obtained by smartphone for gray value analysis and determination of butachlor. The best functional monomers and addition ratio were selected by quantum chemical simulation calculation, the properties of the prepared molecularly imprinted polymers were analyzed, and the detection conditions were optimized. The linear range, sensitivity, and selectivity of the method were evaluated. The results showed that under the optimum conditions, the concentration of 2–80 ng/g had a good linear relationship (R 2 is 0.9953), the detection limit was 1.43 ng/g, the specificity was good, and the whole detection process did not exceed 20 min. The microfluidic paper chip was applied to detect butachlor in mung bean samples. The results showed that the recovery was 93.4–106.4 %, and the relative standard deviation was less than 5.6 %.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Qianqian Geng,Jingchan Xie,Xin Wang,Mengling Cai,Hui Ma,Hanwen Ni
A water-soluble inclusion complex for butachlor was prepared by complexation with (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD). Phase solubility results indicated a 1:1 stoichiometric ratio with an apparent stability constant of 864.3 M–1 in the obtained solid complex. The formation of the complex was confirmed by 1H nuclear magnetic resonance, Fourier transform infrared, and differential scanning calorimetry spectra. Coupled with the molecular docking results, butachlor was considered to be completely included in HP-β-CD cavity. Butachlor complexation with HP-β-CD decreased its adsorption capacity and enhanced its mobility in soil. The inclusion complex displayed better herbicidal activities than free butachlor. The 96 h median lethal concentration values of the inclusion complex and free butachlor was 2.30 and 0.65 mg L–1, respectively, for zebrafish, indicating that the complexation could significantly reduce toxicity to fishes. The present study provides an approach to develop environment-friendly formulations using CDs for herbicides.
研究领域:
环境科学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Jing Yu,Hui Zhu,Heli Wang,Brian Shutes,Tingting Niu
The rapid development of agriculture increases the release of butachlor into aquatic environments. As a dominant species causing cyanobacterial blooms, Microcystis aeruginosa ( M. aeruginosa ) can produce microcystin and poses threats to aquatic ecosystems and human health. However, the impact of butachlor on M. aeruginosa remains unclarified. Therefore, the physiochemical responses of M. aeruginosa to butachlor were investigated, and the relevant underlying molecular mechanism was highlighted. There were no significant changes ( P > 0.05) in the growth and physiology of M. aeruginosa at the low concentrations of butachlor (0–0.1 mg/L), which evidenced a high level of butachlor tolerance in Microcystis aeruginosa . For the high concentrations of butachlor (4–30 mg/L), the inhibition of photosynthetic activity, disruption of cell ultrastructure , and oxidative stress were dominant toxic effects on M. aeruginosa . Additionally, the impaired cellular integrity and lipid peroxidation may be attributed to the substantial elevations of extracellular microcystin-LR concentration. Downregulation of genes associated with photosynthesis, energy metabolism, and oxidative stress was inferred to be responsible for the growth suppression of M. aeruginosa in 30 mg/L butachlor treatment. The upregulation of gene sets involved in nitrogen metabolism may illustrate the specific effort to sustain the steady concentration of intracellular microcystin-LR. These findings dissect the response mechanism of M. aeruginosa to butachlor toxicity and provide valuable reference for the evaluation of potential risk caused by butachlor in aquatic environments.
研究领域:
环境科学
·
毒理学
·
分子生物学
·
水生生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2017
·Vol. 100
·期 2
·208-215
·
IF 1.412
Xiang Qingqing,Xu Bofan,Ding Yilun,Liu Xiaoyi,Zhou Ying,Ahmad Farooq
The widespread contamination and persistence of the herbicide butachlor in the environment resulted in the exposure of non-target organisms. The present study investigated the toxicity effect of butachlor (1–15 µmol/L) and the protective effect of vitamin C (VC) against butachlor-induced toxicity in zebrafish. It was found that butachlor significantly increased the mortality and malformation rates in a dose-dependent manner, which caused elevation in reactive oxygen species (ROS) and malondialdehyde (MDA) after 72 h exposure. Compared with butachlor treatment group, the protective effect of VC against butachlor-induced toxicity were observed after adding 40, 80 mg/L VC respectively. VC significantly decreased the mortality, malformation rates, ROS, MDA, and normalized antioxidant enzymes activities of zebrafish after 72 h exposure. The result shows VC has mitigative effect on butachlor-induced toxicity and it can be used as an effective antioxidant in aquaculture.
研究领域:
环境毒理学
·
水产养殖科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Ru-Yang Zhao,Mu-Zi Li,Qi-Qian Wang,Fu-Wei Jiang,Yi Zhao,Jin-Long Li
Butachlor (BTR), a globally prevalent amide herbicide, poses significant environmental health risks due to its bioaccumulation potential and documented chronic toxicity in mammalian systems. Given the spleen's pivotal role in orchestrating systemic immune homeostasis, this study investigated BTR-induced immunopathological mechanisms in ICR mice following 28-day subacute exposure (10 mg/kg). Histopathological examination revealed significant disorganization of the splenic marginal zone architecture, concomitant with ultrastructural mitochondrial abnormalities and a reduction in mitochondrial membrane potential (ΔΨm). Mitochondrial permeability transition pore opening triggered cytoplasmic translocation of mitochondrial DNA (mtDNA), activating the stimulator of interferon genes (STING) pathway—a master regulator of innate immunity. STING activation initiated bifurcated downstream signaling, evidenced by TBK1 phosphorylation and subsequent IRF3 / NF-κB nuclear translocation. This dual signaling cascade simultaneously executed immunogenic cell death via Gasdermin D-dependent pyroptosis (cleaved GSDMD-NT) and drove macrophage polarization (IFN-α / β, IL-1β, TNF-α). Crucially, pharmacological STING inhibition with C-176 attenuated both pyroptosis (GSDMD cleavage reduced) and inflammatory cytokine surge. Our findings not only pioneer the identification of the mtDNA-STING axis as the central mechanism underlying BTR-induced splenic immunotoxicity but also validate STING inactivation as a targeted strategy to mitigate herbicide-associated immune dysfunction. This work provides critical insights for environmental toxicant risk assessment and immunomodulatory.
研究领域:
环境毒理学
·
免疫学
·
分子生物学
·
药理学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2022
·Vol. 46
·期 1
·2200725
·
IF 3.614
Xinyue Zheng,Xujian Duan,Di Lu,Qiuhong Jiang,Yajun Liu,Hongyu Xue,Jiansong You,Lei Yin,Meiyun Shi
Butachlor is an aromatic amide compound that plays a role as a herbicide, a xenobiotic, and an environmental contaminant. The aim of this work was to develop a highly selective and sensitive ultra-performance liquid chromatography-tandem mass spectrometry method based on the tandem mass spectrometry cubed technique to determine butachlor in a biological matrix. Butachlor and internal standard acetochlor were separated on a Waters Acquity ultra-performance liquid chromatography BEH C 18 column (2.1 × 50 mm, 1.7 μm) with gradient elution using 0.1% formic acid aqueous solution (A) and acetonitrile (B) as mobile phases. The transitions selected for tandem mass spectrometry cubed quantitative analysis in positive ion mode were: for butachlor, mass-to-charge ratio 312.2→238.1→162.1; for acetochlor, mass-to-charge ratio 270.1→224.0→148.1. The total running time for each sample was 5.5 min. The ultra-performance liquid chromatography-tandem mass spectrometry cubed method showed a linear relationship ( R 2 ≥ 0.995) in the concentration range of 0.5–100 ng/ml. The intra and interday accuracies are within the range of -10.6%–4.3% and precisions are between 4.48% and 13.14%. The novelty of the method is the use of tandem mass spectrometry cubed scanning mode, which improves selectivity and sensitivity. The results indicated that butachlor was cellular toxic. The safety of butachlor should be considered when it is used as a herbicide.
研究领域:
分析化学
·
毒理学
·
环境科学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
·
2016
·
IF 3.035
Xiaoyi Liu,Zhaoxing Ling,Xing Zhou,Farooq Ahmad,Ying Zhou
Butachlor is an effective herbicide to deal with undesired weeds selectively and is used at high levels in Asian countries. However, its interaction and impairment effect on BSA was still not clear. In this study, we investigated the interaction between butachlor and bovine serum albumin (BSA) by multi-spectroscopic methods including UV absorption , circular dichroism (CD) spectra, Fourier transform infrared (FTIR) spectra and fluorescence spectra under physiological conditions (pH = 7.4). The results revealed that there was a static quenching of BSA induced by butachlor stemmed from the formation of complex. Based on thermodynamic data, the interaction of butachlor with BSA was due to happen, and van der Waals force as well as hydrogen bond were the major forces contributed to the interaction. The binding constant K b and number of binding site of butachlor with BSA were 5.158 × 10 5 and 1.372 at 303 K, respectively. The distance r between donor (BSA) and acceptor (butachlor) was 0.113 nm, obtained according to the Förster theory. The results revealed that butachlor induced conformational changes in BSA but the secondary structure of BSA was still retained. In addition, the microenvironment around chromophore residues of BSA, for example, tryptophan , changed as well, resulting from the formation of more hydrogen bonds.
研究领域:
生物化学
·
分子药理学
·
药物代谢
·
环境毒理学
·
蛋白质科学
✓ 已验证
DOI
高影响因子
Agronomy-Basel
·
2022
·Vol. 12
·期 11
·2870
·
IF 3.949
Wei Tang,Jinqiu Sun,Xiaoyue Yu,Fengyan Zhou,Shengnan Liu,Mengjie Liu,Yongliang Lu,Yongjie Yang
Roegneria kamojiOhwi (Poaceae), a wild relative plant of wheat which is widely distributed across China, has become a dominant and problematic weed in wheat fields in some regions. We have previously confirmed thatR. kamojiis highly tolerant to foliar-applied acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibitors in wheat (Triticum aestivumL.). The sensitivity ofR. kamojito pre-emergence (PRE) herbicides and the basis of fenclorim increase selectivity to butachlor between wheat andR. kamojiwere evaluated in this study. Screenhouse bioassay showed thatR. kamojiexhibited similar sensitivity to wheat to PRE herbicides at their recommended field doses (RFD); it also showed that buatchlor provides the highest relative control forR. kamoji(53.4% emergence and 81.5% fresh weight reduction, respectively), while it had no impact on seedling emergence of wheat among the six PRE herbicides. When butachlor was applied at four-fold RFD, noR. kamojiseedlings emerged; however, it significantly reduced the above-ground biomass of wheat compared with the non-treated control. Pre-treatment with herbicide safener fenclorim by seed soaking increased the ED10value of butachlor to wheat from 221.8 to 1600.1 g a.i. ha−1, thus increasing the selectivity index from 9.6 to 68.9 between wheat andR. kamoji. The activities of α-amylase activity and protein content during germination, and glutathione-S-transferase (GST) and β-ketoacyl-CoA synthase (KCS) in the seedlings, could be induced by butachlor in both wheat seeds with or without fenclorim pre-soaking. These results suggested that butachlor provides the highest control forR. kamojiand did not affect germination and emergence in wheat. The basis of fenclorim-increased selectivity to butachlor was associated with the induced GST and KCS-mediated enhanced herbicide metabolism in wheat.Keywords:Roegneria kamoji;wheat;herbicide safener;fenclorim;selectivity
研究领域:
农业科学
·
杂草科学
·
植物生理学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2024
·
IF 2.6
Di Lu,Xinyue Zheng,Hongyu Xue,Jiansong You,Lei Yin,Meiyun Shi
The aim of this work was to develop a fast, simple, and reliable UPLC-MS 3 method for the sensitive detection of acetochlor in biological samples. In MS 3 mode, the ion transition m/z 270.1 → 224.1→148.1 was chosen for quantification with butachlor as the internal standard. In the UPLC system, separation was performed on a UPLC column (2.1 × 50 mm ID, 1.7 μm) with 0.1 % FA in water and acetonitrile as mobile phases . After simple protein precipitation via acetonitrile, the method was well validated with good linearity (0.5–20 ng/mL, r > 0.995), accuracy (−3.70 %-2.98 %), and precision (<15 %). The selectivity and sensitivity were improved obviously in MS 3 mode than that in MRM mode. The developed UPLC-MS 3 method was successfully applied to the cellular pharmacokinetics study of acetochlor in MCF-7 cells.
研究领域:
分析化学
·
药理学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Environmental Science
·
2024
·
IF 3.3
Ma Rong,Yu Shuangshuang,Li Yafang,Lin Yan,Ma Xiaodong
Background: With the growing use of nitrogen-containing pesticides in agriculture, their residues in the environment have raised significant public health concerns.Objective: This study aimed to develop a novel PPy-DBSA/IT-SPME coating to enhance the detection efficiency of polar nitrogen-containing pesticides in water matrices.Methods: The preparation conditions were optimized, including pyrrole concentration at 7.0 mol/L, DBSA concentration at 0.014 mol/L, oxidant concentration at 0.35 mol/L, and a coating cycle repeated 10 times. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed for detection.Results: The novel coating effectively adsorbed 19 different categories of polar nitrogen-containing pesticides, including sulfonylureas, triazolopyrimidines, diphenyl ether herbicides, benzoylurea insecticides, and phenylurea herbicides, with unadsorbed rates below 10%. The analytical method achieved an average recovery rate of 61.92% to 115.21%, with an RSD below 5.0%. Detection and quantification limits ranged from 0.012 to 0.524 μg/L and 0.127 to 5.243 μg/L, respectively.Conclusion: The developed method is green, efficient, simple, and cost-effective. It offers an environmentally friendly and user-friendly approach for the detection of polar nitrogen-containing pesticide residues, demonstrating good recovery and precision. This method holds potential for wide application in environmental monitoring and food safety.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 2
·121850
·
IF 7.5
Yong-mei Zhang,Ping-ping Fan,Ling-zhi Zhang,Wei Yu,Song Xia,Fa-li Liu,Xue-de Li
Herbicide is one of the most widely used pesticides in China, yet its spatiotemporal dynamics and associated risks in Chaohu Lake remain poorly understood. Here, we investigated the occurrence, partitioning behavior, and multi-tier risks of three amide herbicides (AHs) and two organophosphorus herbicides (OPHs) in Chaohu Lake, China, using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). All target herbicides were detected in surface water and sediments, showing significantly higher concentrations in summer than in winter and a pronounced spatial gradient, with higher levels in the western than in the eastern lake regions. The majority of the compound was butachlor (BUT), which was 14.950 µg/L in water and 21.909 µg/kg in sediment. The lowest concentration was gluconate-ammonium (GIU), which was 0.050 µg/L in water and 0.570 µg/g in sediment. Sediment-water partitioning coefficients ( K d ) correlated positively with octanol-water distribution coefficient (log k ow ) and total organic carbon (TOC), indicating strong sediment adsorption. Risk assessments revealed that AHs posed high acute ecological risks to aquatic organisms, whereas OPHs showed negligible effects. Human health risk models indicated no significant non-carcinogenic risks (NCR) from acetochlor (ACE), BUT, glyphosate (GLY), or GIU at current levels. This study reported the occurrence of HAs and OPHs in Chaohu Lake for the first time, which filled the data gap of typical herbicides in the ecosystem.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）