Metal-organic frameworks coupling simultaneous saccharication and fermentation for enhanced butyric acid production from rice straw under visible light by Clostridium tyrobutyricum CtΔack::cat1
Here, Metal-Organic Frameworks (MOFs) coupling simultaneous saccharification and fermentation for butyric acid production using rice straw was constructed. Clostridium tyrobutyricum Δ ack :: cat1 , with deleted ack gene and overexpressed cat1 gene, was used as the butyric-acid-fermentation strain. MOFs was employed as a photocatalyst to improve butyric acid production, as well as a cytoprotective exoskeleton with immobilized cellulase for the hydrolysis of rice straw. Thus, the survival of MOFs-coated strain, the thermostability and pH stability of cellulase both remarkably increased. As a result, 55% of rice straw was hydrolyzed in 24 h, and the final concentration of butyric acid in visible light was increased by 14.23% and 29.16% compared to uncoated and coated strain without visible light, respectively. Finally, 26.25 g/L of butyric acid with a productivity of 0.41 g/L·h in fed-batch fermentation was obtained. This novel process inspires green approach of abundant low-cost feedstocks utilization for chemical production.
危险品化学品经营许可证（带存储）