FOOD CHEMISTRY
·
2025
·
IF 9.8
Shufang Yang,Xiaoxue Jiang,Ruoxuan Xu,Yanyan Zhang,Rongxia Liu
Syzygium aromaticum is an aromatic plant that has been widely introduced to tropical regions worldwide. S. aromaticum bud (SAB), as a valuable spice, has been used for centuries, owing to its exceptional health benefits and economic value. The fruit of S. aromaticum (SAF) is not a commonly used component and its phytochemical properties and bioactivities have seldom been analyzed. Herein, ultra-performance liquid chromatography-high-resolution mass spectrometry combined with feature-based molecular networking analysis was applied to characterize the phytochemical constituents of these two parts individually. The bioactivities of SAB and SAF were innovatively evaluated, including their hypolipidemic activity on human hepatocellular carcinoma cells, anti-inflammatory activity on RAW264.7 cells, and anti-proliferative activity on vascular smooth muscle cells. Initially, 75 components from SAB and SAF extracts were identified. Subsequently, activity experiments indicated that both SAB and SAF extracts showed significant activities in these cell types. Intriguingly, they showed similar hypolipidemic effects but different anti-inflammatory and anti-proliferative effects. Then, 14 differential markers were screened for further quantitative analysis to explore the material basis for the difference in bioactivity between SAB and SAF. Finally, using correlation analysis, 10 compounds were identified as biomarkers that contributed to the difference in activity between SAB and SAF. These results suggest that SAF is rich in natural metabolites, showing potential as a natural plant component with hypolipidemic, anti-inflammatory, and anti-proliferative effects. This study could contribute to the targeted selection of different parts of S. aromaticum and serve as a foundation for the development and utilization of SAF.
研究领域:
植物化学
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药理学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2022
·
IF 3.549
Jiaze Yan,Shaomin Ma,Mingrui Feng,Jiqi Zheng,Ming Guo
The use of deep eutectic solvents (DESs) has great prospects because of the green and efficient characteristics, which can be used for developing analytical methods for foods. In this research, assisted by ultrasonic waves, a liquid-liquid microextraction detection method combined with gas chromatography was established for three anaesthetics (eugenol, isoeugenol, and methyl isoeugenol) in aquatic food. The processing conditions including the components, ratio of hydrogen bond acceptor and hydrogen bond donor, DES volume, ultrasonic time, and pH were evaluated and optimised to improve the extraction efficiency, which was based on the DES structures and properties. In-house method validation was carried out by applying to real samples. A Thymol: levulinic acid DES (with a molar ratio of 1:2) was used as the extractant and the recoveries were as high as 93–101% for eugenol, 90–100% for methyl isoeugenol, and 86–94% for isoeugenol with RSDs <5% under optimum conditions. The limit of detection and quantification of the eugenol compounds were 0.08–0.10 μg/mL and 0.26–0.33 μg/mL, respectively. The method has green credentials and comparable LOD to homologous apparatus, which can be used for the determination of eugenol components in aquatic food.
研究领域:
食品科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Huilu Sun,Ruxue Wei,Genqiang Chen,Zhiping Che,Xiaobo Huang,Yingwu Chen,Yuee Tian
Eugenol is the main component of clove basil oil extracted from plants such as Syzygium aromaticum and Ocimum gratissimum . To discover biorational natural product-based pesticides, a series of eugenol/dihydroeugenol derivatives containing carbamate moiety were prepared by introducing an active aminooyloxy group at its C1 hydroxy position, and their structures were well characterized by proton nuclear magnetic resonance ( 1 H NMR), carbon-13 NMR ( 13 C NMR), high-resolution mass spectrometry, and melting point. Bioactivities of these target compounds as anti-oomycete and anti-fungal agents against two serious agricultural pests, Phytophthora capsici and Fusarium graminearum were evaluated. Among assessed compounds: (1) Title compounds 1b and 2b showed significant anti-fungal against F. graminearum , with half maximal effective concentration values of 29.13 and 35.04 mg/L, respectively. (2) The title compounds have obvious selectivity, and their anti-fungal activity is significantly better than their anti-oomycete activity. (3) The anti-oomycete and anti-fungal activities of introducing the aminoalkyloyloxy group at the C1 hydroxyl position of eugenol or dihydroeugenol are significantly better than that of introducing the aminoaryloyloxy group. (4) The presence of a double bond is beneficial for increasing anti-fungal activity, and the reduction of a double bond is beneficial for increasing anti-oomycete activity. Graphical
研究领域:
植物化学
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农药科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Cheng Zhang,Qinghai Zhang,Ming Luo,Qiuping Wang,Xiaomao Wu
Butylated hydroxytoluene (BHT), as an emerging contaminant in ecosystems, has potential influences on animals, aquatic organisms, and public health, and has been proven to be a major allelochemical of Pinellia ternata . In this study, Bacillus cereus WL08 was used to rapidly degrade BHT in liquid culture. Strain WL08 immobilized on tobacco stem charcoal (TSC) particles notably accelerated BHT removal in contract to its free cells, and exhibited excellent reutilization and storage capacities. The optimal removal parameters of TSC WL08 were ascertained to be pH 7.0, 30 °C, 50 mg L −1 BHT and 0.14 mg L −1 TSC WL08. Moreover, TSC WL08 significantly accelerated the degradation of 50 mg L −1 BHT in sterile and non-sterile soils compared to that of free WL08 or natural dissipation, and notably shortened their half-lives by 2.47- or 362.14- fold, and 2.20- or 14.99- fold, respectively. Simultaneously, TSC WL08 was introduced into the continuous cropping soils of P. ternata , which accelerated the elimination of allelochemical BHT, and notably enhanced the photosynthesis, growth, yield, and quality of P. ternata . This study provides new insights and strategies for the rapid in situ remediation of BHT-polluted soils and effective alleviation of P. ternata cropping obstacles.
研究领域:
环境科学
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植物学
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微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Xiuhai Gan,Zhengxing Wang,Deyu Hu
To develop novel antiviral agents, some novel conjugates between ferulic acid and eugenol or isoeugenol were designed and synthesized by the link reaction. The antiviral activities of compounds were evaluated using the half leaf dead spot method. Bioassay results showed acceptable antiviral activities of some conjugates against the tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV). Compounds A9, A10, E1, and E4 showed remarkable curative, protective, and inactivating effects on TMV and CMV at 500 μg mL–1. Notably, these compounds exhibited excellent protective effects on TMV and CMV. The EC50 values of compounds A9, A10, E1, and E4 against TMV were 180.5, 169.5, 211.4, and 135.5 μg mL–1, respectively, and those against CMV were 210.5, 239.1, 218.4, and 178.6 μg mL–1, respectively, which were superior to those of ferulic acid (471.5 and 489.2 μg mL–1), eugenol (456.3 and 463.2 μg mL–1), isoeugenol (478.4 and 487.5 μg mL–1), and ningnanmycin (246.5 and 286.6 μg mL–1). Then, the antiviral mechanisms of compound E4 were investigated by determining defensive enzyme activities and multi-omics analysis. The results indicated that compound E4 resisted the virus infection by enhancing defensive responses via inducing the accumulation of secondary metabolites from the phenylpropanoid biosynthesis pathway in tobacco.
研究领域:
植物化学
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病毒学
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药物化学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Mengyao Gao,Chen Yan,Na Li,Yun Wei
Eugenol has many biological and pharmacological activities such as antioxidant, anticancer, anti-inflammatory, antibacterial and anesthetic. In view of its wide range of beneficial activities, it is crucial to establish a method for the separation and purification of active components from clove. In this study, an electroosmotic coupled with particle-assisted deep eutectic solvent flotation method was established for the enrichment of eugenol from clove. Ten deep eutectic solvent (DES) extraction systems based on menthol as the hydrogen bond acceptor (HBA) were screened, and butanol was determined as the hydrogen bond donor (HBD) to prepare the optimal DES as extractant for extracting eugenol. Under the optimum operating conditions including solid–liquid ratio of 1:30, flotation time of 50 min, nitrogen flow rate of 55 mL/min, salt content of 20 g, pH of the clove suspension of 5.2, applied voltage of 15 V, copper electrode at the bottom, the yield of eugenol was 45.89 ± 1.61 mg/g. Compared with other extraction methods, the extraction rate was greatly improved. The electroosmotic coupled with particle-assisted deep eutectic solvent flotation method provides a green and efficient enrichment strategy for natural product, which has broad application prospects.
研究领域:
化学工程
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药学
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分析化学
·
天然产物研究
✓ 已验证
产品关联
DOI
高影响因子
Toxins
·
2020
·Vol. 12
·期 8
·476
·
IF 3.531
Zhimin Zhou,Renkuan Li,Tzi Bun Ng,Yunyun Lai,Jie Yang,Xiuyun Ye
Aflatoxin B1(AFB1) is a known toxic human carcinogen and can be detoxified by laccases, which are multicopper oxidases that convert several environmental pollutants and toxins. In this study, a new laccase that could catalyze AFB1degradation was purified and identified from the white-rot fungusCerrena unicolor6884. The laccase was purified using (NH4)2SO4precipitation and anion exchange chromatography, and then identified as Lac 2 through zymogram and UHPLC-MS/MS based on the Illumina transcriptome analysis ofC. unicolor6884. Six putative laccase protein sequences were obtained via functional annotation. Thelac 2cDNA encoding a full-length protein of 512 amino acids was cloned and sequenced to expand the fungus laccase gene library for AFB1detoxification. AFB1degradation by Lac 2 was conducted in vitro at pH 7.0 and 45 °C for 24 h. The half-life of AFB1degradation catalyzed by Lac 2 was 5.16 h. Acetosyringone (AS), Syrinagaldehyde (SA) and [2,2′ -azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)] (ABTS) at 1 mM concentration seemed to be similar mediators for strongly enhancing AFB1degradation by Lac 2. The product of AFB1degradation catalyzed by Lac 2 was traced and identified to be Aflatoxin Q1(AFQ1) based on mass spectrometry data. These findings are promising for a possible application of Lac 2 as a new aflatoxin oxidase in degrading AFB1present in food and feeds.Keywords:laccase;Cerrena unicolor;aflatoxin B1;biodegradation;detoxification;product;transcriptomeKey Contribution:A product identified to be AFQ1was found from Lac 2-mediated AFB1degradation, indicating that AFB1would be transformed during Lac 2-mediated AFB1degradation.
研究领域:
微生物学
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生物技术
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环境科学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
Coatings
·
2018
·Vol. 8
·期 3
·88
·
IF 2.35
Jinyue Dai,Shimin Yang,Na Teng,Yuan Liu,Xiaoqing Liu,Jin Zhu,Jun Zhao
In this work, several bio-based main-chain type benzoxazine oligomers (MCBO) were synthesized from eugenol derivatives via polycondensation reaction with paraformaldehyde and different diamine. Afterwards, their chemical structures were confirmed by Fourier Transform Infrared Spectroscopy (FT-IR) and Nuclear Magnetic Resonance Spectroscopy (1H-NMR). The curing reaction was monitored by Differential Scanning Calorimetry (DSC) and FT-IR. The polybenzoxazine films were prepared via thermal ring-opening reaction of benzoxazine groups without solvent, and their thermodynamic properties, thermal stability, and coating properties were investigated in detail. Results indicated that the cured films exhibited good thermal stability and mechanical properties, showing 10% thermal weight loss (Td10%) temperature as high as 408 °C and modulus at a room temperature of 2100 MPa as well as the glass transition temperature of 123 °C. In addition, the related coatings exhibited high hardness, excellent adhesion, good flexibility, low moisture absorption, and outstanding solvent resistance.Keywords:bio-based;benzoxazine;eugenol;coatings
研究领域:
材料科学
·
化学工程
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DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 7
·1-14
·
IF 5.833
Shi Zhaoxia,Xia Ling,Li Gongke,Hu Yufei
Based on platinum nanoparticle–embedded raspberry-liked SiO 2 , a sensitive and selective electrochemical sensor was developed for simultaneous determination of eugenol (EU) and methyleugenol (MEU). Raspberry-liked SiO 2 (RL-SiO 2 ) was characterized with open pores on the surface, which can be used as a path for utilizing the inner space fully. So, platinum nanoparticles (Pt NPs) could be embedded in the inner and outer surface of RL-SiO 2 . As a carrier, RL-SiO 2 not only avoided the agglomeration of the Pt NPs but also improved the catalytic performance. Therefore, the prepared Pt NPs@RL-SiO 2 /GCE exhibited excellent electrocatalytic activity for simultaneous determination of EU and MEU; the linearity ranges were 0.50 ~ 60 μmol/L for EU at a working potential of 0.65 V (vs. saturated calomel electrode) and 0.50 ~ 50 μmol/L for MEU at a working potential of 1.10 V; the detection limits were 0.12 μmol/L and 0.16 μmol/L ( S/N =3); and the relative standard deviations (RSDs) were 3.2% and 4.5%, respectively. In addition, Pt NPs@RL-SiO 2 /GCE was successfully applied to the analysis of fish samples; the obtained recoveries were between 92.0 and 107%. Notably, the results conducted on samples were highly consistent with those obtained from high-performance liquid chromatography. It can be concluded that the study provided a simple method for simultaneous electrochemical determination of EU and MEU in fish samples. Graphical abstract
Schematic illustration of the preparation of RL-SiO2@Pt NPs/GCE for simultaneous determination of eugenol and methyleugenol in fish samples.
研究领域:
分析化学
·
纳米技术
·
电化学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
COMPOSITES PART B-ENGINEERING
·
2020
·
IF 7.635
Jingkai Liu,Jinyue Dai,Shuaipeng Wang,Yunyan Peng,Lijun Cao,Xiaoqing Liu
With the purpose to obtain an eco-friendly flame retardant for epoxy resin, a facile method for the synthesis of a bio-based epoxy monomer containing DOPO units (DGEBDB) from lignin-derived vanillin and guaiacol, was presented in this work. During the synthesis process, guaiacol acted as both reactant and solvent. And it could be recycled in situ and re-used as the fresh one. In order to evaluate the flame retardancy of DGEBDB, it was mixed with diglycidyl ether of bisphenol A (DGEBA) and cured with 4,4-diaminodiphenylmethane (DDM). Results showed that, DGEBDB demonstrated a good compatibility with DGEBA, and the mechanical properties of cured resins were obviously improved with the addition of DGEBDB. As expected, the limiting oxygen index (LOI) of cured resin reached 30.2% with the content of phosphorus only 0.67 wt%, in which the weight ratio between DGEBDB and DGEBA was 1:9 (B1D9/DDM). When the weight ratio of DGEBDB to DGEBA was further increased to 2:8 for the B2D8/DDM system, the UL-94 V-0 grade was achieved. Meanwhile, the mechanism of DGEBDB in improving the flame retardancy of cured resin was discussed and revealed. This easily available and eco-friendly DGEBDB was proved to be a highly efficient reactive flame retardant for epoxy resin.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
Horticulturae
·
2023
·Vol. 9
·期 9
·1024
·
IF 3.1
Xian Zhou,Hui-Hui Ma,Shi-Jie Xiong,Ling-Ling Zhang,Xue-Dong Zhu,Yong-Xing Zhu,Li-Rong Zhou
Fusariumwilt, mainly caused byFusarium oxysporum, affects ginger yield and quality worldwide. To explore a safe and sustainable method of preventing and controllingFusariumwilt, the inhibitory effects of eugenol onF. oxysporum FOX-1were analysed in this study. The results showed that eugenol inhibited the reproductive and vegetative growth ofF. oxysporum FOX-1in vitro. Microscopic observations revealed that eugenol disrupted the hyphal morphology ofF. oxysporum. In addition, eugenol destroyed the cell membrane integrity of the pathogenic fungi, resulting in the leakage ofF. oxysporum FOX-1intercellular contents, including electrolytes, soluble proteins, nucleic acids, and malonydialdehyde. Results of an in vivo inoculation test showed that eugenol exerted a strong inhibitory effect onFusariumwilt in ginger seedlings. In summary, eugenol had an inhibitory effect on the growth ofF. oxysporum FOX-1and controlledFusariumwilt in ginger seedlings. These findings provide a foundation for future development of botanical antifungal agents to manageFusariumwilt.Keywords:Fusariumwilt;eugenol;essential oil;antifungal activity;Zingiber officinale
研究领域:
植物病理学
·
农学
·
植物化学
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DOI
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2015
·Vol. 15
·期 1
·600-605
·
IF 1.556
Jiang Ping,Li Duxin,Xiao Ya,Yang Xingxing,Liu Yuejun
The biodegradable microcapsules based on chitosan for a controlled delivery of clove oil were prepared by the single coagulation process. The effect of chitosan concentration, core to shell ratio, types of emulsifier, flocculating agent and hardening agent on the microcapsule diameter
and the particle size distribution of microcapsule were investigated. The optimized conditions for the preparation of microcapsules with well-defined structure and narrow dispersibility were under that (1) the concentration of chitosan was 1.0 wt%, (2) clove oil to chitosan ratio was 75:25,
(3) OP-10 and 10 wt% sodium sulfate were used as emulsifier and flocculating agent respectively, and (4) the concentration hardening agent glyoxal was 1 wt% based on the weight of chitosan. The uniform spherical structures with smooth surfaces with a particle size distribution of 1–15
μm were evidenced by SEM images of microcapsules. Core–shell, hetero-structures were confirmed by optical micrograph. The chemical component of the microcapsules was determined by FTIR. Thermal analysis showed the microcapsules were thermally stable below 150°C. It was
found that the pH value and temperature play important roles on the release rate of clove oil from the microcapsules. The release volume of clove oil from microcapsules at pH = 7, and pH = 10 were smaller than that at pH = 2. And the release volume of Clove oil from microcapsules at 60°C
was smaller than that at 20°C and 40°C, which showed a sustained and prolonged release.
研究领域:
食品科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）