ANALYTICAL CHEMISTRY
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2024
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IF 6.7
Chen Luo,Youjia Chen,Jinxuan Gu,Huihui Cai,Huijin Lin,Zhigang Jin,Chaobiao Huang
Epinephrine (EP) is an essential catecholamine in the human body. Currently, most EP detection methods are not suitable for in vivo detection due to material limitations. An organic small molecule fluorescent probe based on a chemical cascade reaction for the detection of EP was designed. Anionic heptamethine cyanine dye was selected as a fluorescent dye because of its NIR fluorescence emission with excellent biocompatibility. The secondary amine of EP nucleophilically attacks the carbonate of the probe with its stronger nucleophilicity and further undergoes intramolecular nucleophilic cyclization to release the fluorophore. Other substances containing only primary amines or no β-OH lack reaction competitiveness due to their weaker nucleophilicity or inability to undergo further cyclization. The fluorescence recovery of the probe was linearly related to the EP concentration of 2–75 μmol/L. The detection limit was 0.4 μmol/L. The recovery rate was 94.78–111.32%. Finally, we successfully achieved bioimaging of EP in living cells and EP analogue in nematodes.
研究领域:
分析化学
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生物医学工程
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分子生物学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RESEARCH ON CHEMICAL INTERMEDIATES
·
2016
·Vol. 43
·期 5
·2945-2957
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IF 1.833
Jiang Yichen,Jin Di,Li Yang,Yan Xilong,Chen Ligong
A heptamethine cyanine-based near-infrared (NIR) fluorescent probe Cy-1 for hydrosulfide detection was developed, with the response mechanism based on nucleophilic substitution of acryloyl moiety by HS − . Upon addition of NaHS, Cy-1 displayed a rapid fluorescence quenching (excited at 700 nm) and a quick fluorescence enhancement (excited at 544 nm) as well as obvious changes in absorption spectra and color. The response was fast within tens of seconds and could be observed by naked eyes. By contrasting Cy-1 with the reported probe CyAC , the superiority of Cy-1 in selectivity over Cys was demonstrated. Besides, it also exhibited excellent selectivity and sensitivity to HS − over various anions (0.33-μM detection limit). Additionally, this probe was successfully applied in fetal bovine serum samples and imaging of hydrosulfide in living cells.
研究领域:
化学
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生物化学
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生物医学工程
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细胞生物学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
·
IF 4.831
Jing Liu,Lingfeng Wang,Ronghua Shen,Jie Zhao,Ying Qian
In this study, a new cyanine-based photosensitizer Cy-N-Rh was developed for photodynamic therapy. Based on fluorescence resonance energy transfer (FRET) mechanism, utilizing the absorption of the donor rhodamine (Rh), the acceptor heptamethine cyanine unit (Cy) was indirectly excited to produce singlet oxygen ( 1 O 2 ). The efficiency of energy transfer from the donor Rh to the acceptor Cy was 78.5%. Meanwhile, the singlet oxygen yield of Cy-N-Rh (Φ Δ = 12.00%) was much higher than that of the acceptor Cy (Φ Δ = 4.35%) without FRET. Moreover, the dual cation gave Cy-N-Rh with excellent mitochondria-targeting ability with Pearson’s correlation coefficients of 0.90 and 0.91, respectively. In the MTT test, Cy-N-Rh had low dark cytotoxicity with cell survival rate above 90% and high photo cytotoxicity with cell survival rate below 40%. The cell apoptosis assay also demonstrated the role of the photosensitizer Cy-N-R visually. More importantly, Cy-N-Rh fulfilled two-photon excitation fluorescence imaging under the 800 nm femtosecond laser. All results indicate that this design strategy provides a new method for the development of higher-level cyanine photosensitizers.
研究领域:
生物医学工程
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药学
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细胞生物学
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光化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2023
·
IF 6.7
Peng Chen,Zeng Xiaodie,Cai Jiali,Huang Hanyu,Yang Fang,Jin Shaowen,Guan Xiuhong,Wang Zhiyong
Triple-negative breast cancer is a highly aggressive and metastatic tumor; diagnosing it in the early stages is still difficult, and the prognosis for conventional radio-chemotherapy and immunotreatment is not promising due to cancer’s immunosuppressive microenvironment. The utilization of protein-based nanosystem has proven to be effective in delivering agents with limited adverse effects, yet the combination of diagnosis and treatment remains a difficult challenge. This research took advantage of natural albumin and organic molecules to construct a self-assemble core-shell nanostructure combining with superparamagnetic iron oxide nanocrystals and heptamethine cyanine dye IR780 through non-covalent interactions. This nanocomposite successfully decreased the transverse relaxation time of the magnetic resonance hydrogen nucleus, resulting in outstanding T2 imaging, as well as emitting near-infrared II fluorescence, thereby the resulting dual-modality imaging tool was applied to improve diagnostic competency. It is noteworthy that the nanocomposites exhibited impressive enzyme-like catalytic and photothermal capabilities, resulting in a successful activation of the immune system to efficiently suppress distant metastatic lesions in vivo. Consequently, this nanodrug-based therapy could be an advantageous asset in reinforcing the immune system and hindering the growth and reappearance of the immune-cold breast cancer.
研究领域:
癌症生物学
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纳米技术
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成像科学
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治疗学
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免疫学
✓ 已验证
DOI
PubMed
高影响因子
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