BMC MICROBIOLOGY
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2016
·Vol. 16
·期 1
·1-11
·
IF 2.581
Wang Dan,Zhu Fengqiu,Wang Qian,Rensing Christopher,Yu Peng,Gong Jing,Wang Gejiao
Background Microbial antimonite [Sb(III)] oxidation converts toxic Sb(III) into less toxic antimonate [Sb(V)] and plays an important role in the biogeochemical Sb cycle. Currently, little is known about the mechanisms underlying bacterial Sb(III) resistance and oxidation. Results In this study, Tn5 transposon mutagenesis was conducted in the Sb(III)-oxidizing strain Pseudomonas stutzeri TS44 to isolate the genes responsible for Sb(III) resistance and oxidation. An insertion mutation into gshA, encoding a glutamate cysteine ligase involved in glutathione biosynthesis, generated a strain called P. stutzeri TS44-gshA 540 . This mutant strain was complemented with a plasmid carrying gshA to generate strain P. stutzeri TS44-gshA-C. The transcription of gshA , the two superoxide dismutase (SOD)-encoding genes sodB and sodC as well as the catalase-encoding gene katE was monitored because gshA -encoded glutamate cysteine ligase is responsible for the biosynthesis of glutathione (GSH) and involved in the cellular stress defense system as are superoxide dismutase and catalase responsible for the conversion of ROS. In addition, the cellular content of total ROS and in particular H 2 O 2 was analyzed. Compared to the wild type P. stutzeri TS44 and TS44-gshA-C, the mutant P. stutzeri TS44-gshA 540 had a lower GSH content and exhibited an increased content of total ROS and H 2 O 2 and increased the Sb(III) oxidation rate. Furthermore, the transcription of sodB , sodC and katE was induced by Sb(III). A positive linear correlation was found between the Sb(III) oxidation rate and the H 2 O 2 content ( R
2 = 0.97), indicating that the accumulated H 2 O 2 is correlated to the increased Sb(III) oxidation rate. Conclusions Based on the results, we propose that a disruption of the pathway involved in ROS-protection allowed H 2 O 2 to accumulate. In addition to the previously reported enzyme mediated Sb(III) oxidation, the mechanism of bacterial oxidation of Sb(III) to Sb(V) includes a non-enzymatic mediated step using H 2 O 2 as the oxidant.
研究领域:
微生物学
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环境科学
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DOI
PubMed
高影响因子
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
·
2016
·
IF 3.433
Dong Wang,Kaiwen Guan,Zhiping Bai,Fuqiang Liu
A new facile coating strategy based on the hydrophobicity of methyl groups was developed to prevent nano-sized magnetite particles from strong acid corrosion. In this method, three steps of hydrolysis led to three layers of protection shell coating Fe3O4 nanoparticles. Filled with hydrophobic methyl groups, the middle layer mainly prevented the magnetic core from strong acid corrosion. These magnetite particles managed to resist 1 M HCl solution and 2.5 M H2SO4 solution. The acid resistant ability was higher than those reported previously. After further modification with amino-methylene-phosphonic groups, these magnetite particles successfully adsorbed Sb(III) in strong acid solution. This new strategy can also be applied to protect other materials from strong acid corrosion.
研究领域:
环境化学
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材料科学
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纳米技术
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污染控制
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吸附材料
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DOI
高影响因子
ANALYTICAL LETTERS
·
2021
·
IF 2.329
Zhaoxue Zhang,Yi Lu,Haipu Li,Nan Zhang,Junfei Cao,Bo Qiu,Zhaoguang Yang
The simultaneous separation and determination of inorganic trivalent antimony Sb(III) and pentavalent antimony Sb(V) in plant, soil, and sediment samples was carried out by high performance liquid chromatography – inductively coupled plasma – mass spectrometry (HPLC-ICP-MS). The chromatographic conditions (composition and concentration of competing ion, pH value and flow rate of mobile phase) and the operating parameters for inductively coupled plasma – mass spectrometry were optimized by single factor experiments. The separation of the Sb species was achieved within 6 min using the optimized chromatographic conditions. The detection limits of the method were 0.016 μg/L and 0.021 μg/L for Sb(III) and Sb(V), respectively. The retention times of Sb(V) and Sb(III) were 2.7 min and 4.5 min, respectively. The relative standard deviations for replicate measurements (n = 6) of low (5 μg/L), medium (20 μg/L), and high (40 μg/L) concentrations of Sb(III) and Sb(V) were less than 4%. The results of spiking and recovery experiments for the extracting solutions of soil, sediments, and plants showed that the spiked recoveries of the two species ranged from 92% to 103%. This method has been used to determine the antimony species in extracts of environmental samples.
研究领域:
环境科学
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分析化学
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产品关联
DOI
高影响因子
Advanced Optical Materials
·
2023
·Vol. 11
·期 22
·2300888
·
IF 9
Bihao Zhuang,Zicong Jin,Jiahuan Deng,Hui Xiong,Wenzhe Li,Jiandong Fan
Organic-inorganic hybrid metal halide perovskite has played an important role in optoelectronics and photonics due to their extremely high photoluminescence quantum yield (PLQY). However, considering the strong nonmetallic property of antimony (Sb), the excitons in antimony-based single crystals are usually dissipated in the form of non-radiative recombination under organic-inductive effect, which leads to the low PLQY. Herein, two kinds of perovskite-like single crystals, i.e., (8-HQ) 2 SbCl 3 and (8-HQH)SbCl 4 , are innovatively grown. The density functional theory (DFT) calculations reveal that the unique luminescence characteristics are associated with the inductive effect of organic coordination and exciton effect in the metal octahedral skeleton. When Cl − replaces the organic ligand in (8-HQ) 2 SbCl 3 , the inductive effect is reduced while the self-trapped exciton effect in the metal octahedron is enhanced. Besides, the theoretical research on electron transmission in a modeling device reveals that the hydrogen bond of organic A-site in the single crystal promotes the transition of electrons. Based on the luminescent properties of (8-HQH)SbCl 4 , the white LED devices are obtained by coating (8-HQH)SbCl 4 and red phosphor on 450 nm LED chips.
研究领域:
材料科学
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光电子学
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凝聚态物理
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无机化学
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半导体器件
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光学材料
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晶体工程
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DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 42
·2104607
·
IF 18.808
Meng Qiu,Yanhong Duo,Weiyuan Liang,Yunlong Yang,Bin Zhang,Zhongjian Xie,Xiaoli Yang,Guiqing Wang,Ni Xie,Guohui Nie,Omar A. Alhartomy,Ahmed A. ALGhamdi,Swelm Wageh,Yihai Cao,Han Zhang
Most anticancer drugs with broad toxicities are systematically administrated to cancer patients and their distribution in tumors is extremely low owing to hypoxia, which compromises the therapeutic efficacies of these cancer drugs. Consequently, a preponderant proportion of cancer drugs is distributed in off-target-healthy tissues, which often causes severe adverse effects. Precision cancer therapy without overdosing patients with drugs remains one of the most challenging issues in cancer therapy. Here, a novel concept of nanopoxia is presented, which is a tumor-hypoxia-based photodynamic nanoplatform for the release of therapeutic agents to achieve precision cancer therapy. Under tumor hypoxia, exposure of tumors to laser irradiation induces the fracture of polymer outer shell and produces anticancer reactive oxygen species, and switches 2D antimonene (Sb) nanomaterials to cytotoxic trivalent antimony to synergistically kill tumors. In preclinical cancer models, delivery of Sb nanomaterials to mice virtually ablates tumor growth without producing any detectable adverse effects. Mechanistically, the tumor hypoxia-triggered generation of trivalent antimony displays direct damaging effects on cancer cells and suppression of tumor angiogenesis. Together, the study provides a proof-of-concept of hypoxia-based precision cancer therapy by developing a novel nanoplatform that offers multifarious mechanisms of cancer eradication.
研究领域:
肿瘤学
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纳米技术
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药学
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生物医学工程
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DOI
高影响因子
ENVIRONMENTAL POLLUTION
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2023
·
IF 8.9
Yurong Gao,Hanbo Chen,Zheng Fang,Nabeel Khan Niazi,Kwasi Adusei-Fosu,Jianhong Li,Xing Yang,Zhongzhen Liu,Nanthi S. Bolan,Bin Gao,Deyi Hou,Chenghua Sun,Jun Meng,Wenfu Chen,Bert F. Quin,Hailong Wang
Sorption and oxidation are two potential pathways for the decontamination of trivalent antimony (Sb(III))-bearing water, using iron (Fe)-modified biochar (FeBC). Here we investigated the sorption and oxidation behavior of FeBC for Sb(III) in aqueous solutions. Results revealed that Sb(III) removal by FeBC was significantly improved showing the maximum Sb(III) sorption (64.0 mg g −1 ). Density functional theory (DFT) calculations indicated that magnetite (Fe 3 O 4 ) in FeBC offered a sorption energy of −0.22 eV, which is 5 times that of non-modified biochar. With the addition of peroxymonosulfate (PMS), the sorption of Sb(III) on FeBC was 7 times higher than that on BC, indicating the sorption capacity of FeBC for Sb(III) could be substantially increased by adding oxidizing agents . Electrochemical analysis showed that Fe modification imparted FeBC higher electron-donating capacity than that of BC (0.045 v. s. 0.023 mmol e − (g biochar) −1 ), which might be the reason for the strong Sb(III) oxidation (63.6%) on the surface of FeBC. This study provides new information that is key for the development of effective biochar-based composite materials for the removal of Sb(III) from drinking water and wastewater. The findings from this study have important implications for protecting human health and agriculture.
研究领域:
环境科学
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水处理
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材料科学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
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2021
·
IF 7.086
Guocheng Zhu,Jialin Lin,Qian Yuan,Xiaofeng Wang,Zilong Zhao,Andrew S. Hursthouse,Zhenghua Wang,Qingbo Li
Metal organic framework (MOF) nanoparticles are recognized for their effective removal of metal ions from aqueous systems. However, the application of nanoparticles in a powder form as synthesized is not practical and recovery is not easy. We prepared a recyclable magnetic MOF nanoparticle phase and used a widely available waste biomass to generate biochar to support magnetic nanoparticles applied in the treatment of aqueous antimony pollution. A mushroom waste biochar was used to support a magnetic UIO-66-2COOH (denoted as BSMU). Adsorption of trivalent antimony (Sb (III)) onto the BSMU was evaluated. The results showed that optimum conditions for preparation of the BSMU were the mass ratio of MMOF to biochar 4:1, the temperature 70 °C, the time 4 h, and the initiator 4 mM. Under such conditions, sorption capacity reached 56.49 mg/g for treatment of Sb (III) solution at 100 mg/L and pH 9.1. Alkaline conditions (such as pH 9.1) are more favorable for adsorption than acidic conditions, and coexisting ions including NO 3 − , Cl − , SO 4 2− , and PO 4 3− had no significant negative effect in adsorption, and with the use of low dose, higher adsorption density achieved. The adsorption followed a pseudo second order kinetics model and Freundlich isotherm model. It resulted in a higher enthalpy changes ( Δ H θ ) and activation energy (E a ) of 97.56 and 8.772 kJ/mol, respectively, and enhanced the rate pf random contact between antimony and the BSMU, as indicated by a higher entropy change ( Δ S θ ) up to 360 J/mol·K. As a result, it readily absorbs antimony. These adsorption properties identified in this study would provide a valuable insights into the application of nanoparticles loaded biochar from abundant biomass in environmental remediation.
研究领域:
环境科学
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材料科学
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化学工程
·
废物管理
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DOI
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2023
·
IF 8.7
Yi Lu,Fangyuan Peng,Yingyang Wang,Zhaoguang Yang,Haipu Li
Soil antimony (Sb) pollution is a global concern that threatens food security and human health. Boehmeria nivea L. (ramie) is a promising phytoremediation plant exhibiting high tolerance and enrichment capacity for Sb. To reveal the molecular mechanisms and thus enhance the ramie uptake, transport, and detoxification of Sb with practical strategies, a hydroponic experiment was conducted to compare the physiological and transcriptomic responses of ramie towards antimonite (Sb(Ⅲ)) and antimonate (Sb(Ⅴ)). Phenotypic results showed that Sb(Ⅲ) had a stronger inhibitory effect on the growth of ramie. Root Sb content under Sb(Ⅲ) was 2.43 times higher than that in Sb(Ⅴ) treatment. Based on the ribonucleic acid sequencing (RNA-Seq) technique, 3915 and 999 significant differentially expressed genes (DEGs) were identified under Sb(Ⅲ) and Sb(Ⅴ), respectively. Transcriptomic analysis revealed that ramie showed different adaptation strategies to Sb(Ⅲ) and Sb(V). Key DEGs and their involved pathways such as catalytic activity , carbohydrate metabolisms , phenylpropanoid biosynthesis, and cell wall modification were identified to perform crucial roles in Sb tolerance and detoxification. Two heavy metal-associated domain-type genes, six heavy metal-associated isoprenylated plant proteins, and nine ABC transporters showed possible roles in the transport and detoxification of Sb. The significant upregulation of NRAMP5 and three NIPs suggested their roles in the transport of Sb(V). This study is the basis for future research to identify the exact genes and biological processes that can effectively enhance Sb accumulation or improve plant tolerance to Sb, thereby promoting the phytoremediation of Sb-polluted soils.
研究领域:
环境科学
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植物科学
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分子生物学
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植物修复
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）