JOURNAL OF PEST SCIENCE
·
2025
·
IF 4.1
Wang Jie,Huang He-Xi,Trumble John T.,Wang Chengxing,Zhu Zheng-Yang,Zang Lian-Sheng,Desneux Nicolas,Di Ning,Li Yuan-Xi
Floral resources such as nectar are essential for increasing survival and population growth of synovigenic parasitic wasps in agroecosystems. Although the bottom-up effect of cadmium (Cd) has been identified as a major ecological force influencing multitrophic interactions of synovigenic parasitoids, information on the direct effects of Cd-contaminated floral resources on the fitness of natural enemies which might impact their fitness and effectiveness are still lacking. In this study, we assessed the performance of three commonly used Trichogramma species exposed to Cd-contaminated sucrose solutions. Female survival, longevity, the female/male adult proportion, and F 1 emergence rate of T. japonicum were not affected by Cd concentrations. However, a decline in the survival rate of females, their longevity, and female/male adult proportion were observed for T. dendrolimi at high Cd concentration. No significant differences in female/male adult proporation and longevity of T. ostriniae were found at wide Cd concentration ranges. Our results suggest that Cd-contaminated nectar resources can negatively affect performance of some Trichogramma spp., indicating Cd contamination in nectar would directly reduce the potential value of these species in IPM programs. These results not only increase our understanding of interspecific variations in synovigeny of Trichogramma , but also suggest that releases of T. japonicum and T. ostriniae rather than T. dendrolimi should be considered at Cd-contaminated sites.
研究领域:
环境科学
·
昆虫学
✓ 已验证
产品关联
DOI
高影响因子
SCIENTIA HORTICULTURAE
·
2023
·
IF 4.3
Yuan Zhong,Ming-jie Du,Run-ze Ji,Fang-yun Cheng
Polyploid breeding is one of the most important approaches to improve the traits of cultivated plants, but there has been no feasible method to get polyploid tree peony. In this study, we revealed a sexual triploidization approach from a diploid intersubsectional hybrid tree peony, Paeonia × lemoinei ‘High Noon’, to an allotriploid one, P. × lemoinei ‘Oukan’, by accurate karyotyping based on genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH). The ancestor genomes from P. suffruticosa and P. delavayi , which were defined as A-genome and B-genome respectively, were clearly distinguished by GISH in ‘High Noon’ and ‘Oukan’. Nonhomologous chromosomes were successfully recognized by means of 5S and 45S rDNA FISH. Karyotype comparation based on GISH and FISH indicated that the B-genome and one of the two A-genomes in ‘Oukan’ (2 n =3 x =15, AAB) were highly consistent to the corresponding genomes in ‘High Noon’ (2 n =2 x =10, AB), implying the triploid origination via 2 n gametes produced by first division restitution (FDR). In addition, it was demonstrated for the first time that the dual in series sites of 5S rDNA located on the short arm of chromosome 3 in both A and B-genomes. Besides the familiar strong 45S rDNA signals observed at terminal region of short arms, single weak 45S rDNA signal was newly detected at terminal region in the short arm of certain chromosome 1 in A-genome, and at interstitial-distal region in the long arm of chromosome 5 in both genomes. These findings provide a proof and theoretical support for tree peony polyploid breeding, and an accurate karyotyping method for genetic research of plants in Paeonia .
研究领域:
植物学
·
遗传学
✓ 已验证
DOI
高影响因子
Metabolites
·
2024
·Vol. 15
·期 1
·5
·
IF 3.4
Shicheng Han,Lin Han,Fangying Yuan,Wenzhi Liu,Jing Wang,Xiaofeng Jin,Yanchun Sun
Background : Owing to the progressive rise in saline waters globally, resulting in detrimental impacts on freshwater aquaculture, the underlying molecular distinctions governing the response to alkaline stress between diploid and triploid crucian carp remain unknown. Methods : This investigation explores the effects of 20 and 60 mmol NaHCO 3 stress over 30 days on the gills of diploid and triploid crucian carp, employing histological, biochemical, and multi-omic analyses. Results : Findings reveal structural damage to gill lamellas in the examined tissue. Diploid crucian carp exhibit heightened activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and alkaline phosphatase (AKP), alongside lower malondialdehyde (MDA) and urea nitrogen (BUN) levels compared to triploid counterparts. Metabolomic investigations suggest alterations in purine metabolism, lipid metabolism, sphingolipid metabolism, and aminoglycan and nucleotide sugar metabolism following NaHCO 3 exposure. Transcriptomic data indicate differential expression of genes associated with nitrogen metabolism, complement and coagulation cascades, IL-17 signaling pathways, and Toll-like receptor signaling pathways. Conclusions : Overall, NaHCO 3 -induced stress leads to significant gill tissue damage, accompanied by reactive oxygen species (ROS) production causing oxidative stress and disruptions in lipid metabolism in crucian carp. Furthermore, an inflammatory response in gill cells triggers an immune response. Diploid crucian carp exhibit superior antioxidant and immune capacities compared to triploid counterparts, while also displaying reduced inflammatory responses in vivo. Notably, diploid carp efficiently excrete excess BUN through purine metabolism, mitigating protein metabolism and amino acid imbalances caused by BUN accumulation. This enables them to allocate less energy for coping with external environmental stress, redirecting surplus energy toward growth and development. The above results indicate that diploid organisms can better adapt to saline–alkaline environments. Overall, this study provides novel perspectives into species selection of crucian carp of different ploidy in saline–alkaline waters.
研究领域:
水产养殖
·
环境科学
·
分子生物学
·
生理学
✓ 已验证
DOI
PubMed
高影响因子
EUPHYTICA
·
2024
·Vol. 220
·期 4
·1-13
·
IF 1.6
Zhong Yuan,Du Ming-jie,Ji Run-ze,Rong Qi,Guan Zi-heng,Cheng Fang-yun
Cross-incompatibility is the major challenge in inter-sectional distant hybridization between tree peonies ( Paeonia sect. Moutan ) and herbaceous peonies ( Paeonia sect. Paeonia ). Most of the intersectional cross combinations rarely produce hybrid progenies or even seeds. However, a combination with Paeonia × lemoinei Rehd. ( P. delavayi × P. suffruticosa , tree type) as the pollen parent and P. lactiflora (herbaceous) as the seed parent resulted in many progenies. This research focuses on cytogenetics of two highly compatible intersectional combinations, P. lactiflora ‘Martha W.’ × P. × lemoinei ‘Golden Era’ (‘MW’ × ‘GE’) and P. lactiflora ‘Fen Yun Fei He’ × P. × lemoinei ‘L’Esperance’ (‘FYFH’ × ‘LE’). Karyotype analysis combined with genomic in situ hybridization (GISH) showed that the seed parents, ‘MW’ and ‘FYFH’, are both diploids (2 n = 2 x = 10, CC), while the pollen parents, ‘GE’ and ‘LE’, are tetraploid (2 n = 4 x = 20, AABB) and diploid (2 n = 2 x = 10, AB) respectively. Four progenies from ‘MW’ × ‘GE’, and another four progenies from ‘FYFH’ × ‘LE’, are all triploids (2 n = 3 x = 15, ABC). P. × lemoinei contributes two sets of chromosomes (AB) showing distinct signals in all tested triploids. The essential of a successful intersectional hybridization is to obtain 2 x gametes from tree peony. P. × lemoinei has better compatibility in intersectional hybridization than other tree peony species, probably because of its higher frequency of 2 x gamete (unreduced in diploid, or reduced in tetraploid) generation. Tetraploid tree peonies, such as ‘GE’, might produce abundant 2 x gametes through normal meiosis, which is the possible reason for their better fertility and superior intersectional compatibility than diploid tree peonies. The mechanism of compatibility in intersectional hybridization is supposed to be a rebalance between the distant divergent parents, tree peonies and herbaceous peonies, triggered by ploidy increase of gametes from tree peonies. The discovery of tetraploid tree peony also provides valuable materials to study the mechanism of polyploidization in sect. Moutan , and to promote the distant hybridization breeding in Paeonia.
研究领域:
植物学
·
遗传学
·
植物育种
✓ 已验证
DOI
高影响因子
Analytical Science Advances
·
2025
·Vol. 6
·期 2
·e70037
·
IF 4.1
Dan-ping Liu,Bi-cheng Yang,Qiao-yun Huang,Ping Liu,Hai-yan Luo,Mao-lin Yu,Jian-qiong Yang,An Jia,Yan-qiu Liu,Lin Xu,Bin Hu,Ting Huang
In this work, we developed a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) method to directly analyze human whole blood samples. 1.0 µL of whole blood samples and 1.0 µL of optimized organic matrix were directly loaded onto the sample plates for MALDI-TOF-MS detection. A total of 59 whole blood samples were investigated in this work, including 23 healthy control samples, 28 α-globin gene triplication carriers with α-chain repetition, and eight deletional hemoglobin H disease (HbH) patients with α-chain deficiency. Human hemoglobin (Hb) chains with high signal-to-noise were directly observed. It is found that there is a change in signal ratios of α/β-chains of Hb from blood samples in thalassemia subtypes by comparing the ratios obtained from healthy blood samples. Analytical variability ( n = 6) of signal ratios of α/β-chains was found to be 3.33%–12.86% (intra-day), 4.02%–13.39% (inter-day), and 6.41%–15.32% (inter-laboratory), respectively. Furthermore, the results were validated using different MALDI-TOF MS approaches. Our results suggest that α/β-chains ratios of Hb could be an indicator for investigating thalassemia by MALDI-TOF-MS.
研究领域:
质谱分析
·
临床化学
·
血液学
·
遗传学
·
蛋白质组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）