PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2023
·
IF 4.7
Shaoquan Liu,Jianlu Zhang,Ruijiao Li,Chunyun Zhang,Lixin Wang,Hao Liang,Guangpeng Feng,Dongmei Xiong
Due to chemical and photochemical stability, triazophos has been frequently detected in rivers and oceans over the years with extensive use for pest control in agriculture , and it has become a worldwide ecological concern to the aquatic environment . Until now, fewer data are available regarding the potential long-term adverse effects of triazophos on aquatic invertebrates , which plays an essential role in aquatic food webs , as a key group for water ecosystems. In this experiment, the F 1 - and F 2 progenies of Daphnia magna were recovered when daphnias (F 0 ) exposure to triazophos at environmental-related concentrations (0.1 and 1.0 μg/L) for 21 d; and the indexes related to phenotypic traits, reproduction and gene expression were measured in tested animals. The results showed that heart rate and total number of neonates in exposed F 0 -daphnias were significantly lower than those of control group, and the detoxification genes ( HR96 and P-gp ) were up-regulated while genes related reproduction ( Vtg ) and molting ( Nvd and Shd ) were significantly down-regulated. The heart rate and individual size of F 1 -daphnias (<24 h) were significantly reduced in the treatment group. After 21-d recovery, the heart rate and expression of HR96 , P-gp, Vtg, Nvd and Shd were declined in F 1 -daphnias. There was no obvious difference of morphological traits and heart rate between treatment and control in F 2 -daphnias (<24 h). In summary, daphnias (F 0 ) exposure to triazophos with environmental dose could raise toxic effects on its offspring (F 1 ), which is mainly manifested by reduced heart rate, the accumulated number and individual size of offspring and decreased expression of genes related to molting and reproduction.
研究领域:
环境毒理学
·
水生生态学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2017
·
IF 2.714
Shuang Ma,Jiang He,Mingzhen Guo,Xiaohan Sun,Mingda Zheng,Yingying Wang
A simple colorimetric sensing assay was constructed for quantitative detection of triazophos via aggregation of citrate capped silver nanoparticles (Cit-AgNPs) with an On-Off type. In the presence of triazophos, the Cit-AgNPs was aggregated, resulting in the obvious color change from yellow to prunosus via hydrogen bonding, π-π interactions and substitution. The theoretical investigations between the triazophos and Cit-AgNPs were also validated by density functional theory (DFT) approach. Meanwhile, the calibration curve was found to be linear over the range of 0.1–280 μM and this proposed colorimetric sensing was demonstrated an excellent sensitivity with a detection limit of 5 nM. The recoveries obtained from complex sample matrices were in the range 82%–100.8%. Significantly, the colorimetric probe has been successfully applied for visual detection of triazophos, which validated the excellent potential for real-time and on-site application and possessed considerable advantages of simplicity, rapid detection, portability, cost efficiency and visualization. It can be applied into determining triazophos in environmental samples.
研究领域:
分析化学
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环境科学
·
农药检测
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产品关联
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Han Zhang,Lin Sun,Yuan Zhang,Yan Kang,Huilian Hu,Huirong Tang,Yiping Du
This paper reports accurate synthesis of a new type of surface-enhanced Raman spectroscopy (SERS) substrate based on gold nanoparticles decorated 201 red silanized diatomaceous supports. The developed SERS substrate is easy to fabricate, cost effective and offers sensitive rapid detection. The performance and stability of the SERS substrate was investigated and the results showed good SERS activity of substrate that can last for more than 6 months. Tiazophos and phosmet pesticides in aqueous solutions can be detected clearly at a low concentration of 0.01 mg/L. The new substrate was applied to detect triazophos in apple pulp at 0.2 mg/kg. The results indicate that this substrate have good potential in rapid monitoring of pesticide residues in fruits and could be suitable for field-based applications, and routine laboratory analysis of chemicals.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Qingyi Wei,Chaoying Pan,Tengfei Wang,Hongbin Pu,Da-Wen Sun
High-performance plasmonic substrates have recently attracted much research attention. Herein, a three-dimensional gold nanoparticles (AuNPs) spherical liquid array (SLA) with high “hot spots” and tunable nanometer gap by optimizing the proportion of AuNPs colloids over chloroform was synthesized based on a water–oil interfacial self-assembly strategy. The substrate demonstrated excellent surface-enhanced Raman scattering (SERS) performance using tetrathiafulvalene and rhodamine 6G (R6G) as probe molecules. With a simple extraction and soaking pretreatment process, the SLA exhibited high sensitivity for analysing triazophos on apple peels, with a limit of detection (LOD) of 0.005 µg/mL and recovery ranging from 96 to 110 %. Particularly, the chloroform produced an inherent characteristic peak at 665 cm −1 , which was used as the internal standard to correct SERS signal fluctuation, leading to an improvement of the corresponding coefficient R 2 from 0.97 to 0.99, thus improving the reproducibility. Therefore the SLA substrate possesses the potential for quantitative analysis of food contaminants.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2019
·
IF 3.782
Hong Sihui,She Yongxin,Cao Xiaolin,Wang Miao,He Yahui,Zheng Lufei,Wang Shanshan,Abd El-Aty A. M.,Hacimüftüoglu Ahmet,Yan Mengmeng,Wang Jing
In the present study we have developed a direct competitive CdSe/ZnS quantum dot (QD) fluorescence assay based on micro-array-imprinted membranes for the determination of triazophos in cabbage and apple. The imprinted membranes were directly synthesized on the surface of a 96-well plate by thermal polymerization using triadimefon as the dummy template. Under optimal conditions, the assay showed an excellent linear response over the concentration ranges of 0.1–10,000 μg L−1 with a good coefficient of determination (R2= 0.982). The sensitivity (IC50) and limit of detection (LOD, expressed as IC15) of the developed assay were 3.63 mg L−1 and 0.31 μg L−1, respectively. The applicability of the developed approach was tested for detecting triazophos in incurred samples. The method showed excellent recoveries (109.6–118.9%) and relative standard deviations (RSDs) between 9.9 and 19.5%. The obtained results correlated well with those obtained by LC-MS/MS (R2= 0.9995). The competitive assay using CdSe/ZnS QDs as fluorescence-labeled probe showed good sensitivity, steady and fast response, and excellent anti-interference ability compared to conventional fluorescence-quenching methods. Finally, the feasibility of the proposed methodology was successfully applied for detection of triazophos in real samples.
研究领域:
分析化学
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食品安全
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环境科学
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DOI
PubMed
高影响因子
ACS Agricultural Science & Technology
·
2022
·
IF 0
Qijia Zhang,Tongtong Wang,Guangda Xu,Na Guo,Ce Gao,Peng Song,Lixin Xia
We report a low-cost, shape-adaptable, and stable 3D porous surface enhanced Raman scattering (SERS) film–substrate, on which silver nanoparticles (Ag NPs) were loaded. The SERS substrate could not only be directly immersed in Nile blue A (NBA) solution for detection but also detect triazophos in food by wiping the analytes on solid surfaces. A broad linearity range of 0.2–1 ppb and a low limit of detection of 80 ppt were achieved for a triazophos solution. The substrate was stable and exhibited a high sensitivity after 5 weeks of storage at room temperature. In addition, the substrate has been successfully applied to the detection of practical samples of apples, cabbage, lettuce, and green onions. Thus, this simple and highly efficient SERS substrate may have great potential in pesticide-sensing applications.
研究领域:
食品科学
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分析化学
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材料科学
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DOI
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2019
·
IF 4.527
Guihua Wang,Jian Shao,Manni Wu,Yanxiao Meng,Yasmeen Gul,Hui Yang,Dongmei Xiong
Triazophos (TAP) has become a part of widespread pollutant of the aquatic environment due to its residue. Current study was designed to investigate the toxic effect of TAP at different doses (0.06, 0.3 and 1.5 mg/L) to the model organism of zebrafish ( Danio rerio ) by using multi-endpoint analysis in a 96 h acute exposure test. The direct observation that histological and ultrastructural alteration of zebrafish brain and liver were carried out via paraffin section in hematoxylin and eosin (H&E) staining and transmission electron microscopy (TEM), respectively. In addition, a series of methods were applied for exploring the physiological parameters related to cellular apoptosis. Results indicated that vacuolar structure after 96 h treatment with TAP were appeared in the molecular and granular layers of cerebellum. A large number of nuclear retraction, tissues vacuolation and cytoplasmic loss were observed in liver at histological level. From the fine structural level, the mitochondrial vacuolation and membrane damage of brain cells were found and the cristae of mitochondria disintegrated partly in hepatocytes. Onset of such histological structure alterations were one of the most intuitive reflection to TAP exposure, which needs to analyze biochemical alterations for further study. The mitochondrial membrane potential (MMP) showed a downward trend in the brain and liver of zebrafish. Simultaneously, the activity of caspase-3 and caspase-9 increased after 96 h exposure with a concentration-dependent manner, which could be served as a suitable indicator of cellular apoptosis. Furthermore, apoptosis-related genes ( Apaf-1, p53, Bax, Bcl-2, caspase-3 and caspase-9 ) transcription showed different alterations in response to the TAP treatment. These results indicated that TAP exposure led to apoptosis in zebrafish brain and liver and it was speculated that the apoptosis may occur through mitochondrial pathway. The present study demonstrated that the exposure of zebrafish to the insecticide TAP led to observe its effects at both histological structure and apoptosis level in liver and brain.
研究领域:
毒理学
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环境科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2015
·Vol. 182
·期 15
·2427-2434
·
IF 3.741
Ju Ke-Jian,Feng Jin-Xia,Feng Jiu-Ju,Zhang Qian-Li,Xu Tian-Qi,Wei Jie,Wang Ai-Jun
A nanocomposite consisting of coral-like gold nanostructures on reduced graphene oxide (RGO) was synthesized with the assistance of dimethylbiguanide (DMBG). It was then fabricated on a glassy carbon electrode, coating with cysteamine in order to enable the immobilization of acetylcholinesterase (AChE) as a model enzyme whose activity of hydrolyzing the substrate of acetylthiocholine is inhibited by the pesticide triazophos. The biosensor has response to acetylthiocholine in the 0.3 ~ 300 μM concentration range at 0.65 V (vs. SCE). The inhibition of the enzyme by triazophos can be determined in concentrations of up to 210 ppb, with a detection limit of 0.35 ppb of triazophos ( S / N = 3). The biosensor is highly reproducible and acceptably stable.
Coral-like gold nanostructures supported on reduced graphene oxide were synthesized with the assistance of dimethylbiguanide to fabricate an acetylcholinesterase (AChE) biosensor, which exhibited high reproducibility and good stability, providing a good platform for the detection of organophosphorus pesticides.
研究领域:
生物化学
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环境科学
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纳米技术
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产品关联
DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 45
·4680-4689
·
IF 3.532
Panxue Wang,Xiang Li,Yan Sun,Li Wang,Ying Xu,Guoliang Li
Rapid and reliable detection and quantification of pesticide residues in complex matrices by surface enhanced Raman spectroscopy (SERS) remain challenging due to the low level of target molecules and the interference of nontarget components. In this study, SERS was combined with dispersive liquid–liquid microextraction (DLLME) to develop a rapid and reliable method for the detection of organophosphorus pesticides (OPPs). In this method, DLLME was used to extract and enrich two representative OPPs (triazophos and parathion-methyl) from a liquid sample, and a portable Raman spectrometer was used to analyze the separated sediment using homemade gold nanoparticles colloids as enhancing substrates. The results showed that the developed method displayed good sensitivity and stability for the detection and quantification of triazophos and parathion-methyl with R2 ≥ 0.98. The calculated limits of detection (LODs) in the simultaneous detection of triazophos and parathion-methyl were 2.17 × 10−9 M (0.679 ppb) and 2.28 × 10−8 M (5.998 ppb), and the calculated limits of quantification (LOQs) were 7.23 × 10−9 M (2.26 ppb) and 7.62 × 10−8 M (19.098 ppb), respectively. Furthermore, the developed SERS method was successfully applied to the detection of triazophos and parathion-methyl in apple juice with recoveries between 78.07% and 110.87% and relative standard deviations (RSDs) ≤ 2.06%. Therefore, the developed DLLME facilitated liquid SERS method exhibited good sensitivity and stability for the rapid detection and quantification of OPPs and had the potential to be applied to the rapid detection of OPPs in complex matrices.
研究领域:
分析化学
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环境科学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2019
·
IF 2.697
Guihua Wang,Dongmei Xiong,Manni Wu,Lixin Wang,Jing Yang
Extensive use of triazophos for the chemical control of pests in agriculture or aquaculture might strongly disturb the aquatic environment due to residue accumulation through various routes like surface run-off, spray-drift and effluent from factories, which have potential negative effects to non-target aquatic organisms . Previous studies have documented the antioxidative effects of triazophos to mammals, however, the oxidative toxicity of triazophos to fish has not been adequately studied to date. Thus, an acute exposure (96 h) to triazophos at different concentrations of 0.06, 0.3 and 1.5 mg/L (corresponding to 1/50th, 1/10th and 1/2th of 96 h-LC 50 , respectively), was conducted to investigate the triazophos-induced oxidative stress in adult zebrafish ( Danio rerio ). The results showed that the time- and dose-dependent induction of oxidative stress except for the ROS level in liver after 24 and 48 h exposure, as indicated by increased reactive oxygen species (ROS), malondialdehyde (MDA) level and a compromised antioxidant defense system, including increased superoxide dismutase (SOD) activity, catalase (CAT) activity, glutathione (GSH) content as well as the increased at first and decreased afterwards genes expression ( Sod1 , Sod2 , Cat and Gpx ) in brain. Simultaneously, ROS and MDA showed an increased trend, SOD activity, CAT activity and GSH content showed a trend of increasing at 24 h and decreasing at 48 h, and then increasing at 96 h and Sod1 , Sod2 , Cat and Gpx gene showed decreasing at first and then increasing in liver tissue. The present study concluded that the damage of the antioxidant system by triazophos induced oxidative stress in the brain and liver of zebrafish with concomitant lipid peroxidation , which is an important mechanism underlying the triazophos-induced acute toxicity .
研究领域:
环境毒理学
·
水生生物学
·
鱼类生理学
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产品关联
DOI
PubMed
高影响因子
SENSORS
·
2020
·Vol. 20
·期 15
·4120
·
IF 3.275
Fei Shao,Jiaying Cao,Ye Ying,Ying Liu,Dan Wang,Xiaoyu Guo,Yiping Wu,Ying Wen,Haifeng Yang
For real application, it is an urgent demand to fabricate stable and flexible surface-enhanced Raman scattering (SERS) substrates with high enhancement factors in a large-scale and facile way. Herein, by using the electrospinning technique, a hydrophobic and flexible poly(styrene-co-butadiene) (SB) fibrous membrane is obtained, which is beneficial for modification of silver nanoparticles (Ag NPs) colloid in a small region and then formation of more “hot spots” by drying; the final SERS substrate is designated as Ag/SB. Hydrophobic Ag/SB can efficiently capture heterocyclic molecules into the vicinity of hot spots of Ag NPs. Such Ag/SB films are used to quantitatively detect trace triazophos residue on fruit peels or in the juice, and the limit of detection (LOD) of 2.5 × 10−8M is achieved. Ag/SB films possess a capability to resist heat. As a case, 6-mercaptopurine (6MP) that just barely dissolves in 90 °C water is picked for conducting Ag/SB-film-based experiments.Keywords:electrospun;Ag NPs/poly(styrene-co-butadiene);SERS;triazophos
研究领域:
材料科学
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环境科学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-10
·
IF 5.479
Yang Yong,Cheng Jingli,Wang Biao,Guo Yirong,Dong Xiaowu,Zhao Jinhao
A method is described for simultaneous voltammetric determination of the pesticides triazophos (TRS) and thiacloprid (THD). A glassy carbon electrode (GCE) was modified with a metal-organic framework (type UiO-66-NH 2 ) which has a large specific surface (1018 m 2 ·g −1 ) and contains large amounts of Cd(II) and Pb(II) ions, with adsorption capacities of 230 and 271 mg·g −1 , respectively. The antigen-loaded particles were then bound to antibody, magnetically separated, and analyzed by square wave voltammetry to give signals for Cd(II) and Pb(II) at −0.82 and − 0.56 V (vs. Ag/AgCl) for TRS and THD, respectively. Under optimized conditions, the method has a wide linear range (0.2–750 ng·mL −1 ) and low detection limits (0.07 and 0.1 ng·mL −1 at a S/N of 3 for TRS and THD, respectively). It is perceived that this assay represents a useful tool for simultaneous determination of multiple pesticide residues. The method has a wide scope in that may be extended to monitoring of other small organic pollutants by changing the types of metal ions and by using other antibodies.
Schematic presentation of an amino-modified metal-organic framework (type UiO-66-NH 2 ) loaded with Cd(II) and Pb(II) ions for simultaneous electrochemical immunosensing of triazophos (TRS) and thiacloprid (THD). It is based on the fabrication of antigen (Ab)-immobilized UiO-66-NH 2 -based signal tags (a), and of an antibody (Ab)-immobilized magnetic bead (MB-COOH)-based capture probes (b).
研究领域:
分析化学
·
环境科学
·
生物医学工程
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）