DYES AND PIGMENTS
·
2017
·
IF 3.473
Guangyang Liu,Xiaodong Huang,Shuning Zheng,Lingyun Li,Donghui Xu,Xiaomin Xu,Yanguo Zhang,Huan Lin
We developed a highly sensitive fluorescent assay based on fluorescence resonance energy transfer (FRET) between gold nanoparticles (AuNPs) and cadmium telluride (CdTe) quantum dots (QDs) for detection of triadimenol in tap water and agricultural irrigation water. The electrostatic interaction between the positively charged CdTe QDs and negatively charged AuNPs strongly quenched the fluorescence emission of CdTe QDs. In the presence of triadimenol, the strong electrostatic attraction between negatively charged AuNPs and positively charged triadimenol molecules rapidly induced the aggregation of AuNPs, blocking the FRET reaction and attenuating the quenched efficiency of CdTe QDs. The change of fluorescence intensity was linearly related to the concentration of triadimenol in the concentration range of 0.338–33.8 μM. The detection limit (S/N = 3) of the sensor was as low as 0.182 μM. The fluorescent sensor was successfully used to detect triadimenol in spiked water samples and gave good recoveries.
研究领域:
分析化学
·
环境科学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-13
·
IF 5.3
Shi San-Jun,Ji Mei-Qi,Huang Rong-Fu,Fan Zi-Yan
A general one-step lateral flow immunochromatographic assay (LFIA) for the quantitative detection of triadimefon (TDF) and triadimenol (TDN) in fruit and vegetable samples was developed using time-resolved fluorescence microspheres (TRFM) as labels. A specific anti-triadimefon monoclonal antibody (mAb) was conjugated with TRFM to fabricate LFIA test strips. A time-resolved fluorometer as an LFIA reader was applied to obtain quantitative results and assess risk ranges for the LFIA test strips. Under the optimized experimental conditions, the limits of detection (LODs) in buffer/cucumbers/tomatoes/oranges were 0.046 ng/mL, 0.135 µg/kg, 1.047 µg/kg, and 5.811 µg/kg, respectively, which are ca . 1000 times lower than that of colloidal gold-labeled strips. The recovery in cucumber/tomato/orange samples was 109.4–116.7%, 87.7–110.9%, and 88.0–111.9%, respectively, indicating that the test strips had good reliability. Coupled with the easily customizable pretreatment procedures for various samples, the LFIA results were obtained within 18 min without the need for professional personnel or complicated equipment. TRFM-LFIA for TDF and TDN also shows remarkable specificity and precision. The test strips were also low-cost, portable, and convenient to use. These results indicate the test strips could be utilized as a novel strategy for on-site detection of TDF and TDN, which has the potential to expand and detect other pesticide or insecticide residues in food. Graphical abstract
研究领域:
食品科学
·
分析化学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 27
·期 8
·7823-7834
·
IF 2.914
Wang Zikang,Tian Zhongnan,Chen Li,Zhang Wenjun,Zhang Luyao,Li Yao,Diao Jinling,Zhou Zhiqiang
Reptiles are an important part of vertebrates and are the primitive terrestrial vertebrates. However, lots of reptile species are endangered or susceptible to extinction. It is no doubt that contaminants are one of the important reasons for the decline of the lizard population. In this study, the selective metabolism of triadimenol (TN) in the male Eremias argus lizards and the toxic effects of TN on lizards were studied. TN chiral isomers were separated and detected by HPLC-MS/MS system with Lux Cellulose-1 column. Tissue distribution experiments showed the existence of stereoselectivity biotransformation of TN enantiomers among organs in lizards, and RR-TN preferentially emerged over the other enantiomers. The antioxidant enzymes (SOD, CAT, GST) activities and MDA content assays demonstrated that TN induced oxidative stress in most organs, especially in the liver, and the histopathology analysis showed the severe liver and testis damage caused by 14-day continuous TN gavage. The reproductive effects of TN-induced reflected in the increased sex hormone testosterone. This research confirms that TN could induce hepatic and reproductive toxicity of E. argus lizard.
研究领域:
环境毒理学
·
爬行动物生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Tong Li,Hui Li,Jia Chen,Yongliang Yu,Shuai Chen,Jianhua Wang,Hongdeng Qiu
Chiral enantiomers, especially the enantiomers of chiral drugs often exhibit different pharmacological activity, metabolism and toxicity, thus it is of great research significance to scientifically and reasonably develop single chiral drugs with low toxicity and high efficiency. Among them, high performance liquid chromatographic techniques based on chiral stationary phases (CSPs) has become one of the most attractive methods used to evaluate the enantiomeric purity of single-enantiomers compound of pharmacological relevance. In this work, pillar[5]arene functionalized with L- and D-histidine, respectively, were modified on the surface of mesoporous silica as novel chiral stationary phases called L / D His-BP5-Sil. Notably, L / D -histidine had the characteristics of low steric hindrance and easy derivatization. Although the π-π interaction of imidazole group was weaker than that of benzene ring, the benzene ring bonding imidazole-conjugated ring in the structure produced better enantioseparation effect. The results showed that L / D His-BP5-Sil can separate a variety of complex structural enantiomers with excellent reproducibility, thermal stability and separation performance. Hence, the unique advantage of the highly selective separation of L / D His-BP5-Sil provides new insights into the enantioseparation field.
研究领域:
药物化学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Mingming Chen,Xiaojing Lai,Bihang Su,Xiancai Jiang,Jinhua Xu,Fengfu Fu,Zhenyu Lin,Yongqing Dong
Tebuconazole is one of the most commonly used fungicides in agricultural production, that has the merits of highly effectiveness, broad spectrum and systemic function. Excessive tebuconazole may pose a great threat to human and animal health. Traditional detection techniques for tebuconazole usually have limitations such as expensive equipment, poor antibody stability, and time-consuming procedures. Herein, a sensitive sensor is developed for the rapid detection of tebuconazole based on hydrogel surface-enhanced Raman scattering (SERS) chips. Aggregated Ag nanoparticles (a-AgNPs) with tunable localized surface plasmon resonance (LSPR) wavelength are in-situ synthesized in polyvinyl alcohol (PVA) solution for preparing hydrogel SERS chips. Three hydrogel SERS chips are obtained to match the three commonly used laser wavelengths. On the basis, a match laser wavelength is selected according to the energy levels of tebuconazole and the Fermi level of a-AgNPs to gain a strong chemical enhancement. At the same time, the chip with a corresponding LSPR wavelength to the laser is applied to obtain a strong electromagnetic enhancement. Thus, highly sensitive SERS signal of tebuconazole is obtained. Furthermore, the obtained hydrogel SERS chips have good repeatability, outstanding reproducibility and strong anti-interference ability, and show outstanding reliability in practical applications. As a result, the SERS chips offer a reliable and convenient platform for the quick detection of tebuconazole in foods. The detection limit is as low as 1 ppb, and the recoveries is distributed in the range of 94.66–106.70 %. This work would promote greatly the application of SERS in small molecule detection.
研究领域:
农业科学
·
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Longhui Luo,Dongmei Chen,Xiufang Yan,Chao Kang,Wei Tian,Qian Liu,Xiaoying Yang,Tingting Chen,Youhua Long
Triazole pesticides are widely used to control pathogenic fungi in agricultural production, but the irrational use of these pesticides will have many negative effects on human health and ecological environment. In this study, we constructed a relatively complete visual monitoring system for triazole pesticides based on surface-enhanced Raman spectroscopy (SERS). Silver nanoparticles (Ag@ICNPs) modified with iodide and calcium ions showed stronger SERS enhanced ability and better reproducibility, and the characteristic peaks of eight different triazole pesticides were captured simultaneously. Principal component analysis (PCA) and support vector machine (SVM) were used to identify these pesticides. The developed method has strong quantitative analysis ability, excellent detection sensitivity and wide detection range, and has been successfully applied to the determination of pesticide residues in fruits and vegetables. In addition, dynamic SERS imaging technology combined with multivariate curve resolution-alternating least squares (MCR-ALS) to visually monitor the penetration behavior of triazole pesticides could remove the influence of interfering substances, thus obtaining an accurate distribution map of pesticides, which promoted the quantification of pesticide penetration process. The diffusion ability of different systemic pesticides into fruits and vegetables was revealed. Electrochemical (EC) assisted SERS of triazole pesticides showed that potential-induced molecular reorientation of pesticide molecules on the electrode surface was induced by the polarization of active substrate, which significantly improved the SERS signal of pesticides. Overall, this systematic pesticide monitoring study not only provides an effective innovative scheme for controlling the detection of hazardous substances in agricultural products, but also brings significant prospects for food safety guarantee.
研究领域:
食品安全
·
分析化学
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2026
·Vol. 15
·期 17
·3002
·
IF 6
Min Li,Yulin Wang,Huajuan Yin,Xu Jing,Yunlong Li
Triazole fungicides (TFs) are widely used in cereal production due to their potent fungicidal activity and broad-spectrum efficacy. Nonetheless, residues of TFs in food products may pose risks to food safety and human health. Therefore, the development of efficient and environmentally friendly sample preparation methods is paramount for the reliable determination of TFs. Herein, a novel green solvent-based dispersive liquid–liquid microextraction method coupled with high-performance liquid chromatography (DLLME-HPLC) was developed for the determination of TFs in cereal samples. The prepared magnetic deep eutectic solvents (MDESs), composed of nonanoic acid and ferric hydroxide, served as green, magnetically responsive extraction solvents, enabling rapid magnetic separation without centrifugation. Four bio-based solvents (BBSs) were investigated as green dispersive solvents to facilitate the dispersion of MDESs and replace conventional toxic organic dispersants, thereby further enhancing the environmental sustainability of the extraction procedure. Owing to the combined effects of hydrophobic interactions, hydrogen-bonding networks, and magnetic responsiveness, the proposed method achieved efficient extraction and rapid phase separation while minimizing solvent consumption and operational complexity. The greenness of the method was evaluated using multiple green analytical chemistry metrics, confirming its low environmental impact, reduced waste generation, and improved operational safety compared with conventional DLLME procedures. Under optimized conditions, the method was successfully applied to determine TFs in rice, wheat, corn, buckwheat, and oat samples, achieving recoveries ranging from 75.0% to 101.9% and relative standard deviations of 1.6–4.8%. The developed DLLME method provides a rapid, sensitive, and environmentally friendly strategy for cereal pesticide residue analysis and expands the application of MDESs and BBSs in green sample preparation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jin Liu,Yuanjun Nie,Yu Niu,Li Li,Xu Jing
A universal, green, and rapid lignin-based emulsive liquid-liquid microextraction (ELLME) method was established to detect nine triazole fungicides in water, juice, vinegar , and alcoholic beverages via UHPLC-MS/MS. By employing an environmentally friendly emulsifier (lignin), the proposed ELLME was compatible with more extractants, and not restricted to fatty acids. Due to the high amphiphilic properties and three-dimensional structure of lignin, the emulsion was quickly formed through several aspirate-dispense cycles of the green extractant (guaiacol) and lignin solution. And a micropipette was used for rapid microextraction . The limit of detection was 0.0002–0.0057 μg L −1 . The extraction recoveries and relative standard deviation were 81.7%–102.0% and 0.9%–7.1%, respectively. Finally, three green metric tools were used to verify the greenness of the whole procedure. The proposed lignin-based ELLME successfully emulsified green solvents , indicating that emerging solvents may be excellent alternatives as extractants in ELLME for pesticide residue analysis in food samples.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）