Kang Jiang,Linfang Lu,Jingwen Huang,Jiakun Hong,Yibai Che,Na Li,Xiaoli Li,Shaoze Wu,Zhoujie Xu,Wenjun Yang,Sumei Ling,Shihua Wang
Acifluorfen (AFF), a widely used diphenyl ether herbicide, raises significant environmental concerns due to its persistence and ecotoxicological risks, but the absence of immunoassay methods hinder efficient monitoring. A hybridoma cell line secreting a monoclonal antibody against AFF was developed via hybridoma technology to address this need. Using this antibody, we developed two lateral flow immunoassays (LFIAs) based on gold nanoparticles (AuNP-LFIA) and gold nanoflowers (AuNF-LFIA). The AuNP-LFIA exhibited a limit of detection (LOD) of 75.01 ng/mL. In contrast, the AuNF-LFIA demonstrated significantly enhanced sensitivity, achieving an LOD as low as 7.79 ng/mL, which represents a 9.6-fold improvement over the AuNP-LFIA. When applied to fruit and vegetable samples, both methods showed spike recovery rates ranging from 92.58% to 108.41%, and the results were in good agreement with those obtained by LC-MS. These findings suggest that the developed assays hold strong potential as reliable tools for the on-site detection of AFF.
In this work, a low-cost melamine-formaldehyde polymer with large-surface-area mesoporous structure was prepared and applied as an efficient solid sorbent for pre-concentration of five kinds of chlorinated herbicides including fomesafen, atrazine, pyrithiobac-sodium, benazolin and acifluorfene from water samples. Extraction parameters such as pH of the sample solution, amount of mesoporous melamine-formaldehyde polymer (mMFP), extraction time, type of desorption solv-ent and volume of the sample were systematically investigated. Combining dispersive solid-phase extraction with high-performance liquid chromatography (HPLC), the detection limits (S/N = 3) of the five herbicides were ranged from 0.04 to 0.18 μg L−1 with good linearity (R2 ≥ 0.9908). Then the tap water and river water samples were analysed by the developed method, and the obtained results indicated that this method provides acceptable recoveries and precisions. mMFP can be prepared through one-pot catalyst-free polymerisation from low-cost starting materials, so that they could be easily scaled up for the sample pre-treatment and have the powerful potentiality in purification of contaminated water.
危险品化学品经营许可证（带存储）