Food & Function
·
2021
·Vol. 12
·期 20
·9912-9921
·
IF 5.396
Mohan Zhang,Shuai Chen,Yuhua Dai,Ting Duan,Yuying Xu,Xiaolin Li,Jun Yang,Xinqiang Zhu
Aspartame (ASP) and sucralose (SUC) are non-nutritive sweeteners which are widely consumed worldwide. They are considered safe for human consumption, but their effects on certain physiological aspects, such as the lifespan or health status, of the organism have not yet been studied in depth and only limited data are available in the literature. The objectives of this study were to evaluate the effects of ASP and SUC on the lifespan and health indexes using Caenorhabditis elegans (C. elegans) as a model system. Interestingly, it was shown that at the concentrations tested, ASP (0.03–3 mg mL−1) showed an increasing trend of the mean lifespan of C. elegans, with a significant increase of 27.6% compared to the control at 3 mg mL−1. Similarly, SUC (ranging from 0.03 to 10 mg mL−1) also significantly increased the mean lifespan by 20.3% and 22.3% at 0.03 and 0.3 mg mL−1, respectively. However, 10 mg mL−1 SUC had a negative effect on the lifespan, though it did not reach a statistically significant level. In addition, ASP and SUC decreased lipofuscin accumulation and transiently improved motility, indicating improved health status. Nonetheless, they had different effects on food intake and intestinal fat deposition (IFD) at different intervals of time. Taken together, our findings revealed that ASP and SUC can prolong the lifespan and improve the health status of C. elegans.
研究领域:
生物学
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营养学
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毒理学
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DOI
PubMed
高影响因子
Antioxidants
·
2025
·Vol. 14
·期 4
·474
·
IF 6.6
Jun Hu,Ji Feng,Yan Bai,Zhi-Sheng Yao,Xiao-Yu Wu,Xin-Yu Hong,Guo-Dong Lu,Kun Xue
Background: Sucralose and benzo(a)pyrene (B[a]P) are widespread foodborne substances known to harm human health. However, the effects of their combined exposure on kidney function remain unclear. This study aimed to investigate the mechanisms by which sucralose and B[a]P induce kidney injury through P-glycoprotein (PGP/ABCB1), a crucial protein involved in cellular detoxification. Methods: C57BL/6N mice were co-treated with sucralose and B[a]P for 90 days to evaluate their impact on kidney histopathology and function. In vitro experiments assessed cell viability, reactive oxygen species (ROS) levels, and B[a]P accumulation by flow cytometry. Molecular docking and cellular thermal shift assay (CETSA) were used to determine the binding affinity of sucralose to PGP. Furthermore, PCR, Western blotting, and immunohistochemistry were performed to analyze the expression of PGP and its upstream transcription factors. Results: Ninety days of co-exposure to sucralose and B[a]P significantly exacerbated renal dysfunction in mice, as evidenced by the elevated level of serum creatinine and urea nitrogen, which could be reverted by ROS scavenger N-acetyl cysteine (NAC). In vitro, sucralose promoted cellular accumulation of B[a]P, consequently enhancing B[a]P-induced cell growth inhibition and ROS production. Consistently, B[a]P accumulation was enhanced by PGP knockdown in both HK2 and HEK-293 cells. Mechanistically, sucralose can directly bind to PGP, competitively inhibiting its efflux capacity and increasing intracellular B[a]P retention. Prolonged co-exposure further downregulated PGP expression, possibly through the reductions of its transcriptional regulators (PXR,NRF2, andNF-κB). Conclusions: Co-exposure to sucralose and B[a]P exacerbates renal injury by impairing PGP function. Mechanistically, sucralose inhibits PGP activity, resulting in the accumulation of B[a]P within renal cells. This accumulation triggers oxidative stress and inhibits cell growth, which demonstrates that sucralose potentiates B[a]P-induced nephrotoxicity by directly inhibiting PGP-mediated detoxification pathways, thus underscoring the critical need to evaluate toxicity risks associated with combined exposure to these compounds.
研究领域:
毒理学
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分子生物学
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医学
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DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2021
·
IF 5.909
Yulong Yang,Zhanghua Liu,Huiming Zheng,Shicui Zhu,Kejia Zhang,Xueyan Li,Xiaoyan Ma,Andrea M. Dietrich
As a globally important artificial sweetener, sucralose (SUC) is a persistent emerging contaminant in global aquatic environments. This research comprehensively track the fate and transport of SUC throughout an urban water cycle; to perform laboratory experiments to determine chlorinated disinfection by-products (DBPs) of SUC sebsequent to advanced oxidation with ozone; and to perform a risk evaluation of SUC related DBPs.; The results demonstrated that SUC was consistently present throughout the urban water cycle, including consumer's tap water . The inluent to the municipal wastewater treatment plant contained 1033.4-2626.3 ng/L SUC, and as expected for this non-biodegradable artificial sweetener, the concentration in the effluent was slightly reduced to 917.6-2031.2 ng/L. The concentration of SUC varied in surface waters with a peak value of 2070.0 ng/L. In finished drinking water 288.1–505.3 ng/L SUC was found, and values of 177.7–409.7 ng/L were present in the distribution system. Up to 16.3% of SUC concentration discharged as municipal wastewater was delivered to residents in their drinking water . While conventional treatment little removed SUC, the mean removal efficiency of SUC with ozonation followed by activated carbon filtration was 39.6% at a full-scale drinking water treatment plant. The fate and transformation of SUC in drinking water treated by ozonation and chlorination were investigated in laboratory experiments. Ozonation partially degraded SUC; however, intermediates reacted with chlorine to generate DBPs, including trichloromethane (TCM), dichloroacetic acid (DCAA) and trichloroacetic acid (TCAA). Peak 7-days potential formation of TCM, DCAA and TCAA were 0.016, 0.51 and 23.34 μ g/mg SUC, respectively. DBP yields increased with increasing chlorine dosage, chlorination time, temperature, and solution pH. The presence of ammonia nitrogen in water facilitated dichloroacetonitrile (DCAN) production up to 0.78 μ g/mg SUC, while simultaneously reducing the yield of TCM, DCAA and TCAA. Human exposure analysis revealed that carcinogenic risks of DBPs caused only by SUC ozonation were in the range of 1.86 × 10 −12 -6.12 × 10 −9 , while non-carcinogenic risks were in the range of 6.0 × 10 −9 -4.37 × 10 −6 . The pervasive occurrence of SUC in an urban water cycle, couple to its resistance to biological and chemical treatment, confirms that SUC is a persistent contaminant in the aquatic system, including tap water .
研究领域:
环境科学
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公共卫生
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化学工程
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Liqin Zeng,Jingfeng Gao,Yingchao Cui,Zhiqi Wang,Yifan Zhao,Yukun Yuan,Hongxin Xu,Xiaoyu Fu
Sucralose (SUC), an artificial sweetener widely used in food, beverages and pharmaceuticals, is frequently detected in various environmental matrices. Triclosan (TCS) is commonly used as a disinfectant and often co-exists with SUC in sewage environments. This study investigated the effects of SUC (0.1–10 mg/L) on the transmission of intracellular and extracellular antibiotic resistance genes (ARGs) in the partial nitrification systems with and without TCS pre-exposure. The reactors operated for 150 days, and SUC did not affect ammonia oxidation performance, while TCS led to the maintenance of partial nitrification. The types and abundances of extracellular ARGs in sludge and free ARGs in water increased significantly after TCS pre-exposure when faced SUC stress, which might be caused by a decrease in α-Helix/(β-Sheet + Random coil). SUC was more easily to enrich ARGs in partial nitrification systems with TCS pre-exposure, exacerbating the risk of ARGs transmission. The microbial community showed stronger relationships to cope with the direct stress of SUC, and the functional bacteria ( Thauera and Nitrosomonas ) in TCS pre-exposure system might be potential hosts of ARGs. This study might provide insights for better understanding the fates of SUC in partial nitrification systems and the ecological risks in wastewater containing TCS and SUC. Environmental Implication Sucralose (SUC) is often detected in the environment and considered as an emerging contaminant due to its soaring consumption and environmental persistence. Triclosan (TCS) is an antibacterial agent that often co-exists with SUC in personal care products and sewage environments. During 150 d, two partial nitrification reactors with and without TCS pre-exposure were established to study the effects of SUC on nitrification performance, antibiotic resistance genes (ARGs) and microbial communities. This study showed the refractory nature of SUC, and SUC led to the transmission of extracellular ARGs in partial nitrification system with TCS pre-exposure, exacerbating the risk of ARGs dissemination.
研究领域:
环境科学
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微生物学
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废水处理
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生态毒理学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Jishao Jiang,Huilin Cui,Zhuyu Tang,Shipeng Wang,Ke Cheng,Chunyan Zhang,Tonghuan Yu,Jinghua Lv,Fuqiang Yu,Dong Liu
This study investigates the effects of biochar, laccase, and their combined application on nitrogen transformation, the degradation of sucralose (SUC) and acesulfame (ACE), and the dynamics of bacterial communities during sewage sludge composting. The results indicated that NH 3 emissions were reduced by 41.2 %, 17.5%, and 40.8 % in the biochar, laccase, and biochar-laccase treatments, respectively, compared to the control. Meanwhile, N 2 O emissions decreased significantly by 26.8 % and 16.0 % for the biochar and biochar-laccase treatments, respectively, but increased by 8.1 % for the laccase treatment. Additionally, the biochar, laccase, and biochar-laccase treatments enhanced the degradation of SUC and ACE by 36.6–68.3 % and 49.2–69.8 %, relative to the control, respectively. Network analysis showed that the additive-treatment enhanced the cooperation within bacterial communities, and solidified artificial sweeteners degradation, especially under biochar-laccase application. Phylogenetic investigation of communities by reconstruction of unobserved states further indicated that biochar, laccase, biochar-laccase treatment increased enzymes associated with the degradations of organic matter and artificial sweeteners. In conclusion, it suggested that a combined addition of biochar and laccase was an effectively way to reduce nitrogen loss and promote artificial sweeteners degradations during sewage sludge composting.
研究领域:
环境科学
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微生物学
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废物管理
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生物技术
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DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Jishao Jiang,Rui Hou,Huilin Cui,Zhuyu Tang,Yousif Abdelrahman Yousif Abdellah,Caspar C.C. Chater,Ke Cheng,Fuqiang Yu,Dong Liu
This study surveyed the fates of artificial sweeteners in influent, effluent, and sewage sludge (SS) in wastewater treatment plant, and investigated the effects of Micro-Kaolin (Micro-KL) and Nano-Kaolin (Nano-KL) on nitrogen transformation and sucralose (SUC) and acesulfame (ACE) degradation during SS composting. Results showed the cumulative rate of ACE and SUC in SS was ∼76 %. During SS composting, kaolin reduced NH 3 emissions by 30.2–45.38 %, and N 2 O emissions by 38.4–38.9 %, while the Micro-KL and Nano-KL reduced nitrogen losses by 14.8 % and 12.5 %, respectively. Meanwhile, Micro-KL and Nano-KL increased ACE degradation by 76.8 % and 84.2 %, and SUC degradation by 75.3 % and 77.7 %, and significantly shifted microbial community structure. Furthermore, kaolin caused a positive association between Actinobacteria and sweetener degradation. Taken together, kaolin effectively inhibited nitrogen loss and promoted the degradation of ACE and SUC during the SS composting, which is of great significance for the removal of emerging organic pollutants in SS.
研究领域:
环境工程
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废物管理
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微生物学
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Yuxin Li,Zhiqiu Qu,Chao Shan
Sulfidation and nitridation have been reported as promising methods to modify nanoscale zero-valent iron (nZVI) for water decontamination from chlorinated organic compounds (COCs). However, the effects of sulfidation/nitridation on the reactivity of nZVI towards COCs of different polarities were poorly understood. To explore such effects, nZVI, one-step sulfided nZVI (S 1 -nZVI), two-step sulfided nZVI (S 2 -nZVI), and nitrided nZVI (N-nZVI) were prepared in this study, and chloramphenicol (CAP), florfenicol (FF), and sucralose (SUC) were employed as typical COCs with sequentially increasing polarities. Sulfidation and nitridation significantly enhanced the hydrophobicity of nZVI as observed from water contact angle. Anaerobic condition favored FF degradation by all the four nZVI-based materials, while sulfidation and nitridation could inhibit the competition effect of coexisting nitrate on FF removal by nZVI. The reactivity of the nZVI-based materials towards FF followed the same order of water contact angle, while sulfidation/nitridation did not enhance the reactivity of nZVI towards CAP. Intriguingly, sulfidation/nitridation was found to improve the activity of nZVI for dechlorination reactions during FF and CAP degradation, and N-nZVI exhibited particularly high dechlorination capability (∼90 % for FF and ∼100 % for CAP). In contrast, all the investigated nZVI-based materials had no activity in SUC degradation, indicating the negligible role of sulfidation/nitridation in promoting the reactivity of nZVI towards COCs of strong polarity. In addition, SUC may serve as a recalcitrant target compound to examine the reactivity of modified nZVI materials. This study sheds light on the development of nZVI modification strategies/methods for more efficient water treatment.
研究领域:
环境科学
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水处理
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化学工程
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材料科学
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DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Jingyan Shi,Jianing Li,Wentao Su,Chang Xue,Yuan Zhang,Xinghua Gao
Recent advancements in the food industry have rekindled interest in the safety of food additives, such as sugar substitutes and food pigments. Consequently, the main purpose of this study was to develop models that can more accurately predict the effects of these additives on the human body. In response to this demand, we have created an innovative pancreas islet-on-a-chip system featuring a concentration gradient generator and a perfusable 3D cell culture array. This setup facilitates the 3D culture of pseudo-islets under stable biochemical and biophysical conditions. When compared to static culture environments, our dynamic environment maintains islet cell viability at over 95 %, resulting in larger cell clusters that exhibit a higher tendency for aggregation up to 30 μm. Furthermore, the expression levels of key factors integral to islet development, namely INS-1, INS-2, and PDX-1, increased by 4.5-fold, 1.9-fold, and 5.8-fold respectively in the dynamic environment. Utilizing this sophisticated pancreas islet-on-a-chip model, we discovered that the consumption of sugar substitutes like erythritol and sucralose for 1 h does not impact insulin secretion levels. In contrast, the administration of glucagon-like peptide 1 (GLP-1), GLP-1 receptor (GLP-1R) agonist exendin-4, curcumin, and a combination therapy group led to a substantial increase in insulin secretion levels (p < 0.01). Such engineered microenvironments and pancreatic islet-on-chips offer a groundbreaking platform for evaluating sugar substitutes and antidiabetic compounds.
研究领域:
生物医学工程
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药理学
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内分泌学
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细胞生物学
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药物发现
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DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 13
·2029
·
IF 4.7
Xuening Song,Yingting Luo,Wenduo Zhao,Simiao Liu,Yuzhuo Wang,Hao Zhang
Lutein is an oxygenated fat-soluble carotenoid and a functional compound with proven health benefits for the human body. Nevertheless, the poor water solubility and low oral bioavailability of lutein greatly limit its application. To address this, we developed an effective approach to enhance the water solubility of lutein through co-amorphous formulation. Specifically, the lutein-sucralose co-amorphous mixture was prepared at a molar ratio of 1:1 using ethanol and water as solvents by employing the solvent evaporation method, followed by solid-state characterization and dissolution testing conducted to assess the properties of the formulation. The X-ray diffraction pattern with an amorphous halo and the differential scanning calorimetry thermogram with no sharp melting peaks confirmed the formation of a binary co-amorphous system. Changes in peak shape, position, and intensity observed in the Fourier transform infrared spectroscopy spectrum revealed intermolecular interactions between lutein and sucralose molecules, while molecular dynamics simulations identified interaction sites between their hydroxyl groups. Additionally, dissolution testing demonstrated better dissolution performance of lutein in the co-amorphous form compared to pure lutein and physical mixture counterparts. Our findings present a novel strategy for improving the water solubility of lutein to make better use of it.
研究领域:
药学
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材料科学
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分析化学
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生物物理学
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DOI
PubMed
高影响因子
THERIOGENOLOGY
·
2024
·
IF 2.4
Wenjiao Liu,Han wang,Qi Mu,Ting Gong
Taste receptor type 1 subunit 3 (T1R3) is a G protein-coupled receptor encoded by the TAS1R3 gene that can be specifically activated by certain sweeteners or umami agents for sweet/umami recognition. T1R3 is a potential target for regulating male reproduction. However, studies on the impact of non-nutritive sweeteners on reproduction are limited. In the present study, we evaluated the impact of the non-nutritive sweeteners (saccharin sodium, sucralose and acesulfame-K) on testosterone synthesis in testicular Leydig cells of Xiang pigs by comparing the relative abundance of mRNA transcripts and protein expression of T1R3, steroidogenic related factors, and intracellular cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), as well as testosterone levels using Western blotting, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA). To clarify the specific mechanism, a dual luciferase assay was used to uncover the relationship between the transcription factors and steroidogenic enzyme. The acute intratesticular injection of a typical non-nutritive sweeteners was conducted to verify this impact in mouse. The results showed that saccharin sodium not only enhanced T1R3 expression in Leydig cells of Xiang pigs, but also caused significant increases in testosterone, cAMP, PKA, phosphorylation of specificity protein 1 (p-SP1), total protein of specificity protein 1 (SP1), steroidogenic acute regulatory protein (StAR), and 3β-hydroxysteroid dehydrogenase type 1 (3β-HSD1) ( P <0.05). Similarly, treatment of Leydig cells with sucralose and acesulfame-K also increased testosterone level, protein expression of T1R3, 17-α-hydroxylase/17, 20-lyase (CYP17A1), and 3β-HSD1 ( P <0.05). Treatment with SQ22536 (an adenylate cyclas inhibitor) or H89 (a PKA inhibitor) significantly reduced saccharin sodium-induced protein levels of p-SP1, StAR, CYP17A1, and 3β-HSD1 ( P <0.05). In addition, a dual luciferase assay further demonstrated that SP1 significantly increased the promoter activity of CYP17A1 ( P <0.05). When mouse testes were injected with saccharin sodium, T1R3, p-SP1, CYP17A1, and 3β-HSD1 were upregulated, leading to a significant testicular increase in testosterone and cAMP levels ( P <0.05). These results suggest a mechanism by which the taste receptor T1R3 regulates testosterone production, and this mechanism may be linked to the cAMP-PKA pathway. Understanding the interrelationship between T1R3 and the cAMP-PKA-SP1 pathway contributes to clarify the regulatory mechanisms of male reproduction.
研究领域:
生殖生物学
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内分泌学
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分子生物学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Lele Chen,Guanchi Liu,Wei Su,Xiaoxue Hu,Jianshe Chen,Yue He,Xinmiao Wang
This study investigated the formation of aerosols containing non-volatile flavor compounds, specifically sucralose, during oral processing of model wine and their potential retro-nasal perception. It was hypothesized that non-volatile flavor compounds can be delivered via the oro-nasal route in the form of aerosols, generated via various oral movements, and contributing to retro-nasal sensory perception. In vitro experiments demonstrated that the total quantity of generated aerosol particles increased significantly with higher sucralose concentrations in the model wine ( p < 0.001). Carefully-designed sensory evaluations, including A-not-A, check-all-that-apply (CATA), and scaling tests, were conducted to assess retro-nasal perception. The results confirmed that sucralose aerosols could trigger sensory perception during oral processing of model wine. Notably, blocking the retro-nasal olfactory route via nose-clipping led to a substantial decrease in discrimination accuracy (42 % → 13 %, p < 0.01), confirming the perceptual relevance of this transport pathway. This study is the first to demonstrate that liquid aerosols containing non-volatile flavor compounds can be formed during wine drinking and transported to the retro-nasal region, highlighting their potential role in an aerosol-mediated pathway for flavor sensation.
研究领域:
食品科学
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感官科学
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分子感官学
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口腔生理学
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风味化学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Yueyi Zhang,Shi Luo,Guodong Cao,Xin Song,Zhiqiang Xiong,Lianzhong Ai,Guangqiang Wang
D-allulose, erythritol, and sucralose showed distinct intestinal responses. • Sucralose showed the weakest support for beneficial bacterial growth in vitro. • Sucralose caused greater intestinal perturbation under inflammatory stress. • Sucralose-associated microbiota remodeling coincided with elevated p-cresyl sulfate. • p-cresyl sulfate altered inflammatory and barrier-related markers in Caco-2 cells. Sugar substitutes used in reduced-sugar foods differ in chemical structure, digestive fate, and microbial accessibility, yet their intestinal responses have rarely been compared under a common exposure framework. D-allulose, erythritol, and sucralose were evaluated as distinct sugar substitutes, with inulin included as a reference prebiotic carbohydrate. The ingredients were comparatively evaluated under a standardized exposure framework using in vitro fecal fermentation, healthy mice, and mice subjected to dextran sulfate sodium (DSS)-induced inflammatory stress. Gut microbiota profiling was integrated with inflammatory and barrier-related measurements, followed by serum metabolomics and Caco-2 validation of the sucralose-associated response. In vitro, D-allulose and inulin supported greater growth or enrichment of selected potentially beneficial bacteria, whereas sucralose showed the weakest growth support. In healthy mice, sucralose reduced microbial diversity and increased serum lipopolysaccharide by 23.2% relative to Control, accompanied by focal inflammatory cell infiltration and lower ZO-1 expression. Under DSS challenge, sucralose was associated with the greatest intestinal perturbation, including a 20.0% shorter colon than in the Model group, higher disease activity index and colonic myeloperoxidase levels, enhanced pro-inflammatory responses, and reduced tight-junction- and mucus-related markers. These changes coincided with enrichment of Faecalibaculum , depletion of Akkermansia and Dubosiella , and elevation of a serum feature annotated as p-cresyl sulfate. In Caco-2 cells, p-cresyl sulfate at 5 and 50 μM altered inflammation- and barrier-related markers without reducing cell viability. Overall, these findings demonstrate ingredient-specific intestinal response profiles and support considering gut microbial and intestinal effects alongside technological and nutritional attributes when evaluating sugar substitutes for reduced-sugar food applications. Download: Download high-res image (140KB) Download: Download full-size image
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高影响因子
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