TOXICOLOGICAL SCIENCES
·
2017
·Vol. 161
·期 2
·431-442
·
IF 4.081
Wang Jia,He Huan,Xiang Chen,Fan Xiao-Yang,Yang Li-Yun,Yuan Lian,Jiang Feng-Lei,Liu Yi
As a novel delocalized lipophilic cation, F16 selectively accumulates in mitochondria of carcinoma cells and shows a broad spectrum of antiproliferative action towards cancer cell lines. In order to reveal the mode of action and molecular mechanism of F16 inducing cytotoxicity, we investigated the effects of F16 on cancer cells and isolated mitochondria relative to its precursor compound (E)-3-(2-(pyridine-4yl)vinyl)-1 H-indole (PVI), which has a similar structure without positive charge. It was found that PVI did not accumulate in mitochondria, and exhibited lower cytotoxicity compared to F16. However, when they were directly incubated with mitochondria, both F16 and PVI were observed to induce damage to mitochondrial structure and function. Moreover, it was found that F16 as well as PVI acted as uncouplers on mitochondria, and further rescue experiments revealed that the addition of adenosine 5′-triphosphate was the most effective way to recover the cell viability decreased by F16. Thus it was concluded that the decreased intracellular adenosine 5′-triphosphate availability induced by the uncoupling effect of F16 was a major factor in F16-mediated cytotoxicity. Futhermore, the results indicated that the uncoupling effect of F16 is attributed to its chemical stucture in common with PVI but independent of its positive charge. The study may shed light on understanding the underlying mechanism of action for F16, and providing suggestions for the design of new mitochondria-targeted antitumor molecules.
研究领域:
肿瘤学
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分子生物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Binghuan Huang,Zhirong Geng,Shihai Yan,Zan Li,Jun Cai,Zhilin Wang
Adenosine triphosphate (ATP) is used as the energy source in cells and plays crucial roles in various cellular events. The cellular membrane is the protective barrier for the cytoplasm of living cells and involved in many essential biological processes. Many fluorescent probes for ATP have been successfully developed, but few of these probes were appropriate for visualizing ATP level fluctuation in cell membranes during the apoptotic cell death process. Herein, we report the synthesis of a new water-soluble cationic polythiophene derivative that can be utilized as a fluorescent sensor for detecting ATP in cell membranes. Poly((3-((4-methylthiophen-3-yl)oxy)propyl)triphenylphosphonium chloride) (PMTPP) exhibits high sensitivity and good selectivity to ATP, and the detection limit is 27 nM. The polymer shows low toxicity to live cells and excellent photostability in cell membranes. PMTPP was practically utilized for real-time monitoring of ATP levels in the cell membrane through fluorescence microscopy. We have demonstrated that the ATP levels in cell membranes increased during the apoptotic cell death process. The probe was also capable of imaging ATP levels in living mice.
研究领域:
生物材料
·
生物化学
·
细胞生物学
·
分子生物学
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生物医学工程
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DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 41
·25746-25752
·
IF 3.108
Li Xu,Qin Xu,Xiaoyu Guo,Ye Ying,Yiping Wu,Ying Wen,Haifeng Yang
Alumina is widely recognized as chemically inert, and resistant to oxidation and high temperature. In this study, Au/Al2O3 nanocomposites (NCs) were prepared by a facile method and were used as a surface enhanced Raman scattering (SERS) substrate for detection of adenosine triphosphate (ATP). This Au/Al2O3-based SERS substrate demonstrated good sensitivity with the lowest detectable concentration of 5 × 10−9 M for ATP and a good linear relationship ranging from 5 × 10−5 to 5 × 10−9 M. The strategy of improvement of SERS detection sensitivity for ATP was based on the consideration of ATP captured by such SERS substrate via chelation of aluminum and phosphates, which was validated by X-ray photoelectron spectroscopy. In addition, alumina as a supporting material could be expected to prolong the stability of such a SERS substrate.
研究领域:
纳米技术
·
分析化学
·
材料科学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Haiyan Zhao,Linna Dou,Jujie Ren,Min Cui,Na Li,Xueping Ji,Xiaoting Liu,Cong Zhang
Adenosine triphosphate (ATP), as the primary energy source and significant biomarker, is associated with the occurrence and progression of various diseases. Therefore, detecting ATP sensitively is essential for ATP-related biochemical study, disease diagnosis and treatment. Herein, sensitive electrochemical aptasensing of ATP was proposed based on MOF-derived porous Co 3 O 4 coupled with AuNPs and nucleic acids as a novel electrocatalysis signal probe . Firstly, [email protected] 3 O 4 nanocomposite was synthesized via pyrolysis Co-zeolitic imidazolate framework and then functionalized with AuNPs by in situ chemical reduction, which showed excellent electrocatalytic activity towards hydroquinone (HQ) as well as excellent stability. Then, DNA capture probe (CP) was connected to [email protected] 3 O 4 nanocomposite via Au-S bond to form [email protected] 3 O 4 -CP signal probe for signal transduction . Meanwhile, well-designed aptamer probe (AP) containing ATP aptamer and its complementary sequence was immobilized on AuNPs modified electrode surface via Au-S bond. In the presence of ATP, the aptamer in AP interacted with ATP, resulting in the dissociation of AP. Consequently, [email protected] 3 O 4 -CP signal probe could be captured on electrode surface via hybridization, leading to a significant amplified electrocatalytic signal towards HQ. Finally, the quantitative determination of ATP was achieved by determining the current signal generated on electrode surface. The proposed electrochemical aptasensor demonstrated excellent sensitivity and selectivity that could detect ATP as low as 0.3 nM. Moreover, the aptasensor showed excellent performance for detecting ATP in complex serum matrix. These results indicate that MOF-derived nanocomposites have promising application in construction of novel electrocatalysis signal probe as well as versatile bioassays.
研究领域:
分析化学
·
电化学
·
生物传感器
·
分子生物学
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产品关联
DOI
高影响因子
CELL CALCIUM
·
2018
·
IF 3.718
Bin Liu,Wanxin Cao,Jiping Li,Jun Liu
Adenosine triphosphate (ATP) is stored as lysosomal vesicles in marginal cells of the stria vascular in neonatal rats, but the mechanisms of ATP release are unclear. Primary cultures of marginal cells from 1-day-old Sprague–Dawley rats were established. P2Y 2 receptor and inositol 1,4,5-trisphosphate (IP3) receptor were immunolabelled in marginal cells of the stria vascular. We found that 30 μM ATP and 30 μM uridine triphosphate (UTP) evoked comparable significant increases in the intracellular Ca 2+ concentration ([Ca 2+ ] i ) in the absence of extracellular Ca 2+ , whereas the response was suppressed by 100 μM suramin, 10 μM 1-(6-(17β-3-methoxyester-1,3,5(10)-trien-17-yl)amino)-hexyl)-1H-pyrrole-2,5-dione(U-73122), 100 μM 2-aminoethoxydiphenyl borate (2-APB) and 5 μM thapsigargin (TG), thus indicating that ATP coupled with the P2Y 2 R-PLC-IP3 pathway to evoke Ca 2+ release from the endoplasmic reticulum (ER). Incubation with 200 μM Gly-Phe-β-naphthylamide (GPN) selectively disrupted lysosomes and caused significant increases in [Ca 2+ ] I ; this effect was partly inhibited by P2Y 2 R-PLC-IP3 pathway antagonists. After pre-treatment with 5 μM TG, [Ca 2+ ] i was significantly lower than that after treatment with P2Y 2 R-PLC-IP3 pathway antagonists under the same conditions, thus indicating that lysosomal Ca 2+ triggers Ca 2+ release from ER Ca 2+ stores. Baseline [Ca 2+ ] i declined after treatment with the Ca 2+ chelator 50 μM bis-(aminophenolxy) ethane- N , N , N ʹ, N ʹ-tetra-acetic acid acetoxyme-thyl ester (BAPTA-AM) and 4 IU/ml apyrase. 30 μM ATP decrease of the number of quinacrine-positive vesicles via lysosome exocytosis, whereas the number of lysosomes did not change. However, lysosome exocytosis was significantly suppressed by pre-treatment with 5 μM vacuolin-1. Release of ATP and β-hexosaminidase both increased after treatment with 200 μM GPN and 5 μM TG, but decreased after incubation with 50 μM BAPTA-AM, 4 IU/ml apyrase and 5 μM vacuolin-1. We suggest that ATP triggers Ca 2+ release from the ER, thereby contributing to secretion of lysosomal ATP via lysosomal exocytosis. Lysosomal stored Ca 2+ triggers Ca 2+ release from the ER directly though the IP3 receptors, and lysosomal ATP evokes Ca 2+ signals indirectly via the P2Y 2 R-PLC-IP3 pathway.
研究领域:
细胞生物学
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神经科学
·
生理学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2023
·
IF 4.2
Xinxin Ke,Yi Hu,Chuanxia Chen,Tao Hu
Clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostics have been considered as the next generation molecular diagnosis tool. Single-readout mode has been extensively employed in massive CRISPR/Cas12a-based biosensors. In this work, we propose a one-tube dual-readout biosensor for ultrasensitive nucleic acids and non-nucleic acids detection by harnessing CRISPR-ALP tandem assay for the first time (termed CRISAT). In the presence of target, the Cas12a is activated to randomly cut the single-stranded hyDNA sequence of MB@hyDNA-cALP, thus releasing abundant alkaline phosphatase (ALP) in the supernatant solution. By using 4-aminophenol phosphate as the substrate of ALP, p-aminophenol is produced and then reacts with N-[3-(trimethoxysilyl)propyl]ethylenediamine or diethylenetriamine to generate silicon-containing polymer carbon dots (Si PCDs) or polymer carbon dots (PCDs) in situ, which can be observed by naked eye or detected by a fluorescent device in the same solution. Using this strategy, a fluoroscence and colorimetry dual-readout nanoplatform for CRISPR-based biosensor can be rationally developed. We ascertain the applicability of CRISAT through detecting SARS-CoV-2 pseudovirus, achieving superior sensitivity and specificity. With simple modification of crRNAs, the CRISAT platform can be also employed to detect monkeypox virus (MPXV) and non-nucleic acids of adenosine triphosphate (ATP). This work provides a great potential for nucleic acids and non-nucleic acids detection.
研究领域:
分子诊断
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生物传感器
·
纳米技术
·
病毒学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 145
·期 14
·5032-5040
·
IF 3.978
Jie Lv,Shuangling Wang,Cong Zhang,Yulong Lin,Yan Fu,Meng Li
Breaking the pH limitation of the enzyme-like activity of nanomaterials is of great importance for extending their applications in environmental and biomedical fields. Herein, to mimic the role of histidine residues in horseradish peroxidase (HRP), adenosine 5′-triphosphate (ATP) is reported to improve the peroxidase-like activity of hollow Prussian blue nanocubes (hPBNCs). Due to the inherited porous structures, hPBNCs can expose all the binding sites as far as possible to ATP to significantly amplify their catalytic activity and broaden their applicable pH range up to pH 12. Introduction of ATP provides the possibility of realizing efficient catalytic reactions under alkaline conditions. Upon binding with hPBNCs, ATP can enhance the stability of hPBNCs, increase the affinities of the catalysts towards substrates and improve the conductivity of hPBNCs as well as change the decomposed product from H2O2. Moreover, on the basis of the different catalytic activities of hPBNCs towards ATP, adenosine 5′-diphosphate and adenosine 5′-monophosphate, hPBNCs–ATP is utilized to construct a novel colorimetric sensor for the detection of alkaline phosphatase (ALP) activity in biological fluids, which is significantly important for the clinical diagnosis of ALP-related diseases.
研究领域:
纳米材料
·
生物化学
·
催化
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2021
·Vol. 25
·期 3
·1073-1081
·
IF 2.647
Jin Xin,Lv Mengmeng,Pan Qing,Fang Shu,Zhu Ningning
We describe a label-free electrochemical aptasensor for adenosine triphosphate (ATP) assay based on bifunctional Fe 3 O 4 @Au nanocomposites. Due to the introduction of Au nanoparticles, the resulting bifunctional Fe 3 O 4 @Au nanocomposites can be easily modified with ATP-specific aptamer molecules through Au–S interaction to build the nanoprobe and used to immobilize on a glassy carbon electrode simply with the assistance of a magnet. Once exposed to the target ATP, the current signal obtained at nanoprobe-containing magnetic glassy carbon electrode decreases immediately. The decrease in sensor current is a result of the less methylene blue adsorbed on the aptamer caused by the fact that the ATP-specific aptamer prefers to bind with ATP rather than with methylene blue. The decrease in sensor current can be used for electrochemical determination of ATP, for example, to determine ATP in human serum samples.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
电化学
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DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 92
·75746-75752
·
IF 3.84
Siqi Zhang,Kun Wang,Jiali Li,Zhenyu Li,Ting Sun
In this study, a highly efficient colorimetric assay for adenosine triphosphate (ATP) was constructed based on a split aptamer target binding strategy and grapheme oxide–platinum/gold nanoparticle platform to increase catalytic properties. Platinum/gold nanoparticles were easily prepared by reducing the mixture solution of K2PtCl6 and HAuCl4 using sodium citrate in a one-pot synthesis. Next, graphene oxide/platinum/gold nanoparticle hybrids (GO/PDDA/PtAuNPs) were fabricated using a self-assembly procedure. Ultrasensitive and enzyme-free detection of ATP in fetal bovine serum was achieved using split aptamer-modified magnetic beads as a separating unit and split aptamer-modified GO/PDDA/PtAuNPs as a catalyst-detecting platform. The specific binding of ATP and its aptamer linked the split aptamer-modified GO/PDDA/PtAuNPs and magnetic beads together. Using magnetic separation, achromatous tetramethylbenzidine was catalyzed into a colored product by separated nanocomposites, which enabled rapid detection of ATP. The color change caused by the low concentration ATP (50 nM) was clearly distinguished by direct observation. The detection limit of the colorimetric assay for ATP was 0.2 nM, which is close to the lowest detection limit for colorimetric detection of ATP. This assay showed excellent selectivity against guanosine triphosphate, uridine triphosphate, and cytosine triphosphate and can be applied in real samples.
研究领域:
分析化学
·
纳米技术
·
生物化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Guangxu Fang,Kai Wen,Qixuan Zhao,Yingxuan Chen,Yanbo Wang,Haobo Han,Quanshun Li
Postoperative tissue analysis is essential for the evaluation of surgical outcome in the tumor therapy. The identification of tumor biomarkers represents a supplementary approach to histochemical observation, facilitating the diagnosis of cancers at molecular level. Herein, a biosensor was developed by integrating an ATP-responsive aptazyme and its substrate on the surface of streptavidin magnetic beads to achieve the simultaneous detection of ATP and apurinic/apyrimidinic endonuclease 1 (APE1) within cancer cells. This strategy enabled the biosensor to respond to excessive ATP in cancer cells, thereby generating the primer. Meanwhile, the biosensor contained a double-stranded DNA with apurinic/apyrimidinic site, which allowed it to respond to APE1 and produce a padlock. The released primer and padlock triggered the rolling cycle amplification to yield an amplified fluorescent or colorimetric signal. The biosensor functioned as the “AND”-logic gate, effectively distinguishing cancer and non-cancerous cells. Moreover, the biosensor exhibited high specificity and sensitivity in discriminating the tumor and non-tumor tissues isolated from the patients bearing non-small cell lung cancer. Taken together, the biosensor showed the potential in clinical molecular diagnosis, serving as a promising supplementary approach for the postoperative tissue analysis.
研究领域:
生物医学工程
·
分子生物学
·
癌症诊断
·
分析化学
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产品关联
DOI
高影响因子
NPG Asia Materials
·
2014
·Vol. 6
·期 7
·e114
·
IF 9.902
Lin Youhui,Huang Yanyan,Ren Jinsong,Qu Xiaogang
There has been great interest in the development of artificial inorganic nanomaterials that mimic natural peroxidases. Unfortunately, these nanomaterials usually possess relatively low catalytic activity and are generally considered to work effectively only within a narrow temperature range (for example, they are inactive at high temperatures). Intriguingly, adenosine triphosphate (ATP) is not only the ubiquitous energy currency of life, it is also known to form charge transfer complexes with aromatic molecules and to participate in free radical redox chemistry. Inspired by its unique properties, for the first time, we reveal a novel catalytic role for ATP and show that ATP is an ideal boosting agent for markedly improving the catalytic activity of a peroxidase mimic over a broad temperature range and, more significantly, making it possible to achieve exceptionally efficient high-temperature catalytic reactions. These observations pave the way for identifying highly effective modulators that promote the overall performance of artificial enzymes.
研究领域:
生物化学
·
催化作用
·
纳米材料
·
酶工程
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产品关联
DOI
高影响因子
Science Advances
·
2024
·Vol. 10
·期 28
·
IF 11.7
Hanrong Li,Yifan Li,Lingpu Zhang,Nan Wang,Dong Lu,Dongsheng Tang,Yitong Lv,Jinbo Zhang,Heben Yan,He Gong,Ming Zhang,Kaili Nie,Yi Hou,Yingjie Yu,Haihua Xiao,Chaoyong Liu
Celastrol (CEL), an active compound isolated from the root of Tripterygium wilfordii, exhibits broad anticancer activities. However, its poor stability, narrow therapeutic window and numerous adverse effects limit its applications in vivo. In this study, an adenosine triphosphate (ATP) activatable CEL-Fe(III) chelate was designed, synthesized, and then encapsulated with a reactive oxygen species (ROS)–responsive polymer to obtain CEL-Fe nanoparticles (CEL-Fe NPs). In normal tissues, CEL-Fe NPs maintain structural stability and exhibit reduced systemic toxicity, while at the tumor site, an ATP-ROS–rich tumor microenvironment, drug release is triggered by ROS, and antitumor potency is restored by competitive binding of ATP. This intelligent CEL delivery system improves the biosafety and bioavailability of CEL for cancer therapy. Such a CEL-metal chelate strategy not only mitigates the challenges associated with CEL but also opens avenues for the generation of CEL derivatives, thereby expanding the therapeutic potential of CEL in clinical settings.
研究领域:
药理学
·
纳米技术
·
癌症研究
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）