JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2023
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IF 4.3
Yonglin Li,Shengmin Zhou,Yaoyao Dong,Qi Li,Yuan Gao,Xiuzhu Yu
Triterpene alcohols with a series of bioactivities are important minor compounds in camellia oil. Thus, the determination and characterization of triterpene alcohols in camellia oil are essential to the development of camellia oil bioactivity. Herein, a method that simultaneously determines 11 triterpene alcohols was developed and validated; samples from different production areas and oil processing methods were determined, and the storage and thermal stability of triterpene alcohol were characterized. Results showed that the proposed method had good sensitivity, accuracy, and precision, with linearity, LOD, LOQ, spike recovery, and RSD of 0.9964–0.9989, 0.14–5.53 mg/kg, 0.45–18.44 mg/kg, 82.90% 108.51%, and 4.38% 12.69%, respectively. Oils processed with low press flux, cold press, and aqueous extraction had high triterpene alcohol content. Triterpene alcohols showed fluctuation similar to sterols. They were stable overall during storage, and were degraded during heating. Our study can be used as a reference for triterpene alcohol determination and development of camellia oil products containing high content of triterpene alcohols.
研究领域:
食品科学
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分析化学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
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IF 4
Yuan Gao,Na Zhang,Xingyue Li,Linhang Qu,Yonglin Li,Xiuzhu Yu
Walnut oil, well-known for its nutritional value, is a promising source of healthy lipids. This study assessed the physicochemical properties and levels of volatile compounds, glycerides, tocopherol, phytosterols, and triterpene alcohol in 31 commercial walnut oil samples from different countries: China (with 27 samples), France (with 3 samples), and Germany (with 1 sample). The percentage content of unsaturated fatty acids in walnut oils exceeded 90 %. A total of 93 volatile compounds were identified, and the corresponding concentrations ranged from 28.02 to 528.37 mg/L, including 22 aldehydes, 14 acids, 22 alcohols, 10 alkanes, 13 ketones, 4 alkenes, 6 esters, and 2 pyrazines. 58 glycerides were identified, with concentrations of 568.59–2056.53 mg/kg. TG (54:2), TG (54:3), and TG (54:7) were the top three glycerides. Five types of phytosterols were identified, with levels of 568.48 mg/kg - 2364.68 mg/kg. γ-tocopherol was the most abundant tocopherol, ranging from 51.18 mg/kg to 1832.55 mg/kg. Total triterpene alcohol ranged from 106.73 to 717.64 mg/kg, including taraxerol, cycloartenol, and parkeol, etc . The results indicated that there were slight differences in the chemical composition of walnut oils among different regions or countries. Notably, walnut oil was a good source of unsaturated fatty acids, phytosterols, and tocopherols.
研究领域:
食品科学
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营养学
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化学分析
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
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2023
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IF 2.8
Qingfeng Xie,Zhuo Cao,Weiling You,Xiaoping Cai,Mei Shen,Zhangyong Yin,Yiwei Jiang,Xin Wang,Siyu Ye
New anti-lung cancer therapies are urgently required to improve clinical outcomes. Since ganodermanontriol (GDNT) has been identified as a potential antineoplastic agent, its role in lung adenocarcinoma (LUAD) is investigated in this study. Concretely, lung cancer cells were treated with GDNT and/or mycophenolate mofetil (MMF), after which MTT assay, flow cytometry and Western blot were conducted. Following bioinformatics analysis, carboxylesterase 2 (CES2) was knocked down and rescue assays were carried out in vitro. Xenograft experiment was performed on mice, followed by drug administration, measurement of tumor growth and determination of CES2, IMPDH1 and IMPDH2 expressions. As a result, the viability of lung cancer cells was reduced by GDNT or MMF. GDNT enhanced the effects of MMF on suppressing viability, promoting apoptosis and inducing cell cycle arrest in lung cancer cells. GDNT up-regulated CES2 level, and strengthened the effects of MMF on down-regulating IMPDH1 and IMPDH2 levels in the cells. IMPDH1 and IMPDH2 were highly expressed in LUAD samples. CES2 was a potential target for GDNT. CES2 knockdown reversed the synergistic effect of GDNT and MMF against lung cancer in vitro. GDNT potentiated the role of MMF in inhibiting tumor growth and expressions of CES2 and IMPDH1/2 in lung cancer in vivo. Collectively, GDNT suppresses the progression of LUAD by activating CES2 to enhance the metabolism of MMF.
研究领域:
肿瘤学
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药理学
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分子生物学
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细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
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