ANALYTICAL LETTERS
·
2021
·
IF 2.329
Minting Wang,Liting Zhao,Yanmei Wen,Yulian Wu,Yubin Li
Simian virus 40 (SV40) causes multiple diseases due to its low immunogenicity and strong infectivity. Here, the development of a label-free fluorescent sensor designed to detect double-stranded DNA (dsDNA) via a functionalized molecular beacon (MB) produced using triplex DNA and guanine (G)-quadruplexes is described. A SV40-specific dsDNA sequence was used as the target dsDNA (T-dsDNA). The MB was designed to comprise a G-quadruplex sequence, a complementary homopyrimidine sequence for T-dsDNA, and a partially complementary sequence for G-quadruplex sequence. Addition of T-dsDNA forces the MB to unfold, forming triplex DNA, and releasing the G-quadruplex sequence that binds to N-methyl mesoporphyrin IX (NMM), producing a signal. The G-quadruplex/NMM complex augments the signal at 610 nm and allows qualitative SV40 detection. Under optimal conditions, this strategy demonstrates a low limit of detection (0.4 nM). Compared to traditional biosensors, this sensor is enzyme-free, rapid, selective, sensitive, and was used to determine T-dsDNA in serum, suggesting that this strategy has applications in clinical analysis and for other viruses.
研究领域:
生物化学
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病毒学
·
分析化学
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DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Xiaojuan Liu,Ningning Xu,Panpan Gai,Feng Li
Since melamine is a strong hazard to human health, the development of new methods for highly sensitive detection of melamine is highly desirable. Herein, a novel fluorescent biosensing strategy was designed for sensitive and selective melamine assay based on the recognition ability of abasic (AP) site in triplex towards melamine and signal amplification by Mg 2+ -dependent DNAzyme. In this strategy, the melamine-induced formation of triplex DNA was employed to trigger the strand displacement reaction (SDR). The SDR process converted the specific target recognition into the release and activation of Mg 2+ -dependent DNAzyme, which could catalyze the cleavage of fluorophore/quencher labeled DNA substrate (FQ), resulting in a significantly increased fluorescent signal. Under the optimal conditions, the fluorescent signal has a linear relationship with the logarithm of the melamine concentration in a wide range of 0.005–50 μM. The detection limit was estimated to be 0.9 nM (0.1ppb), which is sufficiently sensitive for practical application. Furthermore, this strategy exhibits high selectivity against other potential interfering substances, and the practical application of this strategy for milk samples reveals that the proposed strategy works well for melamine assay in real samples. Therefore, this strategy presents a new method for the sensitive melamine assay and holds great promise for sensing applications in the environment and the food safety field.
研究领域:
生物化学
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食品安全
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分析化学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Ruidi Shen,Li Zou,Shixin Wu,Tingting Li,Jing Wang,Jianmin Liu,Liansheng Ling
A novel label-free fluorescent sensor for histidine was developed based upon Cu 2+ -specific DNAzyme, hybridization chain reaction(HCR) and triplex DNA . Cu 2+ can bind to the histidine, in the presence of histidine, leading to the inhibition of the cleavage of substrate strand of Cu 2+ -dependent DNAzyme, then the intact substrate strand trigger the HCR between H1 and H2. The HCR product can be recognized by triplex-forming oligonucleotide (TFO) through triplex formation and reported by the fluorescence of berberine , the fluorescence intensity of the sensing system was proportional to the concentration of histidine during the range of 5.7–455 nmol L −1 , with a detection limit of 2.0 nmol L −1 .
研究领域:
生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Jing Wang,Tingting Li,Haigang Li,Gongke Li,Shixin Wu,Liansheng Ling
AuNPs-DNA probes remain a challenge to recognize the double-stranded DNA (dsDNA) from routine PCR product despite its success in the recognition of the single-stranded (ssDNA) DNA of asymmetry-PCR product. Here, a universal colorimetric PCR biosensor is established with oligonucleotide modified AuNPs by recognizing the dsDNA of PCR product based on triplex DNA formation with the assistance of a triplex DNA intercalator Ru(phen) 2 dppx 2+ . The strategy was applied to detect different length dsDNA, including short dsDNA 1 (49 bp) and long dsDNA 2 (321 bp), both of which had the good linear relationship between the absorbance and the logarithm of the concentration of the targets. Moreover, the limit of detection (LOD) for short and long length dsDNA was 0.5 pM and 0.05 fg, lower than those of the previously reported methods, respectively. This strategy also displayed high selectivity against dsDNA 1 and dsDNA 2. These results demonstrate a potential strategy to detect all length of dsDNA, providing new opportunities in the life science, diagnosis, and medicine.
研究领域:
生物化学
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分子诊断
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纳米医学
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产品关联
DOI
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2017
·Vol. 34
·期 1
·28-32
·
IF 1.024
Zhang Hong,Zou Li,Li Ruimin,Zhao Mingqin,Ling Liansheng
Simian virus 40(SV40) is a polyomavirus and can induce a series of different tumors. The recognition of SV40 genome is crucial to tumor diagnosis and gene therapy. Herein, a sensitive and selective colorimetric method for sequence-specific recognition of homopyrimidine·homopurine duplex DNA(dsDNA) of SV40(4424—4440, gp6) was established with a hairpin probe based upon the formation of triplex DNA. Hairpin probe 5′-CCC TAC CCA TTT TTT CTT CTC TTT CCT GGG TAG GGC GGG TTG GG-3′(HP) containing G-rich sequence and 17-bp trip-lex-forming sequence was used as the signal probe, which was stem-loop structure alone and exhibited low catalytic activity. Upon its binding to the target duplex of SV40, hairpin probe transferred from stem-loop structure to parallel triplex DNA, accompanied by the recovery of catalytic activity of DNAzyme and a sharp increase of absorbance. Under optimum conditions, the absorbance was increased proportionally to the concentration of dsDNA over the range from 500 pmol/L to 40.0 nmol/L with a detection limit of 433 pmol/L. Moreover, satisfied results were obtained when the assay was used to recognize the mismatched sequences.
研究领域:
分子生物学
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肿瘤诊断
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基因治疗
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DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 36
·3598-3604
·
IF 3.532
Wenxiu Huang,Lizhen Zhao,Ruidi Shen,Gongke Li,Liansheng Ling
A highly sensitive and selective RGB color analysis for the detection of formaldehyde (FA) was developed by using a DNA functionalized gold nanoparticle (AuNPs-DNA) probe. When complementary oligonucleotides (oligo 2 and oligo 3) and a silver ion (Ag+) were added to the AuNPs-DNA solution, triplex DNA was formed, resulting in the aggregation of AuNPs, and accompanied by a solution color change from red to purple. With the addition of formaldehyde, it reacted with Ag+, decreased the stability of triplex DNA between AuNPs–DNA, induced the dispersion of AuNPs, and the color of AuNPs recovered to red. Therefore, the formaldehyde concentration could be estimated with the RGB (red, green, blue) values of the AuNP solution by using a smartphone application (APP). The R value of the system was proportional to the concentration of formaldehyde within the range of 0.23–4.50 mg L−1, with a detection limit of 0.14 mg L−1. The method has been successfully applied to detect the residues of formaldehyde in vegetable samples and has the potential of the on-site determination of formaldehyde.
研究领域:
生物化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·4490-4490
·
IF 4.259
Xiaojuan Liu,Mengmeng Song,Feng Li
Melamine detection has attracted much attention since the discovery of the damage of melamine to human health. Herein, we have developed a sensitive homogeneous electroanalytical platform for melamine detection, which is relied on the formation of triplex molecular beacon integrated with exonuclease III (Exo III)-mediated signal amplification. The formation of triplex molecular beacon was triggered by the recognition and incorporation of melamine to the abasic (AP) site contained in the triplex stem. The stem of the triplex molecular beacon was designed to have a protruding double-strand DNA, which can be recognized and hydrolyzed by Exo III for releasing methylene blue (MB)-labeled mononucleotide. These released MB molecules exhibit high diffusivity toward indium tin oxide electrode with negative charge, thus producing a significantly increased electrochemical response. Taking advantages of the high binding affinity of the DNA triplex structure containing AP sites towards melamine and the unique features of Exo III, this sensing platform is capable for sensitive and selective melamine assay with a detection limit as low as 8.7 nM. Furthermore, this strategy shows good applicability for melamine assay in real samples. Therefore, this strategy broadens the application of triplex DNA and presents a new method for sensitive detection of melamine.
研究领域:
分析化学
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生物传感
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食品安全
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DOI
PubMed
高影响因子
ChemistrySelect
·
2022
·Vol. 7
·期 42
·e202203178
·
IF 2.307
Yuanxiang Fu,Hengdan Si,Juan Chen,Wenjuan Zhang,Shuang Feng,Zhiyou Xiao
Graphical Triplex DNA plays an essential role in molecular recognition, gene regulation, diagnosis, and treatment of genetic diseases. It is crucial to screen intercalators to enhance triplex DNA stability. We established a novel “turn-on” fluorescence sensor for screening triplex DNA binders based upon molecular beacon and target dsDNA. The working principle of DNA fluorescence sensor was confirmed by circular dichroism experiments and melting curve tests. Triplex DNA plays an essential role in molecular recognition, gene regulation, diagnosis, and treatment of genetic diseases. However, triplex DNA has some shortcomings, such as poor internal Hoogsteen hydrogen bond stability and the difficult formation of parallel triplex DNA under physiological conditions, limiting its applications to a certain extent. Therefore, it is crucial to screen intercalators to enhance triplex DNA stability. Herein, we established a novel “turn-on” fluorescence sensor for screening triplex DNA binders based upon molecular beacon. Eight different organic small molecules were screened. The fluorescence of the sensor was significantly enhanced in the presence of berberine, coralyne, palmatine, and neomycin. These four small molecules affect stabilizing triplex DNA as an intercalator; the other four small molecules, kanamycin, tetracycline, ethidium bromide, and 4 ′, 6-diamidino-2-phenylindole did not show any noticeable intercalation effect. The results of circular dichroism and melting curve were consistent with those of the fluorescence spectrum.
研究领域:
生物化学
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分子生物学
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遗传学
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DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2016
·
IF 3.169
Xiaoling Xu,Fang Gao,Xincai Xiao,Yan Hu,Chaozhen Zhu,Dan Zhao
The identification of spatial structures of drugs and the researches on their interaction mechanism with DNA are always attractive to the researchers. However, their realization is lack of simple and fast method. This paper reports the establishment of multiple-functional detection platform based on the “turn off-on” model of ZnCdSe quantum dots. In this system, ZnCdSe quantum dots work as the fluorescent probe, platinum anti-cancer drugs as the quencher and triplex DNA as the trapping agent. The seemingly similar cisplatin and transplatin exhibited different fluorescent recovery behaviors due to their difference in structure, and thus realized the selective detection of cisplatin and transplatin with the reaction time set at 10 min as well as the quantitation of cisplatin over the range of 2.5 × 10 −8 –100 × 10 −8 M. Based on this, the interactions between platinum anti-cancer drugs and ctDNA as well as polymorphic DNA were further studied, and realized the recognition of triplex DNA. The multiple-functional detection platform integrates the functions of the filtration of high-efficient platinum anti-cancer drugs, the researches on interaction mechanism of drugs, and the recognition of polymorphic DNA, meaningful to the future treatment of viral and cancers based on antisense gene strategy.
研究领域:
化学
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生物化学
·
分子生物学
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纳米技术
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癌症研究
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Mengyan Wang,Ying Li,Chi Zhang,Gaiping Li,Lina Zou
A sensitive signal-on photoelectrochemical aptasensor for antibiotic determination was constructed based on the energy level matching between ferrocene and CuInS 2 . P-type CuInS 2 microflower was complexed with reduced graphene oxide (CuInS 2 /rGO) to get photocathode current with good photoelectric conversion efficiency and stability. Then, hairpin DNA (HP) was covalently bonded to the electrode surface. A triple helix DNA (THMS) was used as a molecular switch. After the specific recognition between target and THMS in homogeneous solution, ferrocene labeled probe (Fc-T2) was released. Finally, Fc-T2 was captured by the HP, which leaded the obvious increase of photocurrent for the energy level matching between ferrocene and CuInS 2 . The increase of the photocurrent signal was proportional to the concentration of target amoxicillin (AMOX), the linear range was 100 fM-100 nM with detection limit of 19.57 fM. Meanwhile, the method has been successfully applied for milk and lake water samples analysis with satisfactory results.
研究领域:
分析化学
·
生物化学
·
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Wanying Ye,Xiaohui Chen,Xu Li,Yueming Liu,Fan Jia,Qiao Jin,Jian Ji
The size of the nanocarrier is considered one of the most important issues for its therapeutic effect. Thus, an intelligent nanocarrier with dynamic size has been explored as a promising approach to fulfill the requirements for both efficient accumulation according to the enhanced penetration and retention (EPR) effect and deep penetration into tumor tissue. Herein, structure-switchable triplex DNA was modified on gold nanoparticles (AuNPs) to investigate its potential to modulate the nanoparticle dynamic disassembly process among the tumor microenvironment. We report that the pH-sensitive triplex DNA exhibited outstanding sensitivity and size tunability in triggering the disassembly of AuNP clusters into smaller sizes among the tumor acidic environment, leading to better permeability both in vitro and in vivo. By further combination of the telomerase-sensitive hairpin DNA loaded with chemotherapy drug doxorubicin (DOX), a cancer-specific intracellular drug-release function was also realized, resulting in a precise treatment effect and lower toxicity on normal cells. Through comodification of these two structure-switchable DNA chains on AuNPs and construction of nanoparticle assemblies with proper size, programmed disassembly and drug-release function in tissue and cell level, respectively, were successfully combined and eventually facilitated a highly efficient nanodrug transportation process, from tumor accumulation to deep penetration and precise cancer chemotherapy. The study provided the prospect of utilizing functionalized DNA in optimization of nanocarrier delivery efficiency.
研究领域:
纳米医学
·
分子生物学
·
癌症治疗
·
生物化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2013
·Vol. 405
·期 18
·5993-5999
·
IF 3.659
Li Xue-Mei,Song Jian,Cheng Tao,Fu Pei-Yu
A duplex–triplex switchable DNA nanomachine was fabricated and has been applied for the demonstration of intracellular acidification and apoptosis of Ramos cells, with graphene oxide (GO) not only as transporter but also as fluorescence quencher. The machine constructed with triplex-forming oligonucleotide exhibited duplex–triplex transition at different pH conditions. By virtue of the remarkable difference in affinity of GO with single-stranded DNA and triplex DNA, and the super fluorescence quenching efficiency of GO, the nanomachine functions as a pH sensor based on fluorescence resonance energy transfer. Moreover, taking advantage of the excellent transporter property of GO, the duplex–triplex/GO nanomachine was used to sense pH changes inside Ramos cells during apoptosis. Fluorescence images showed different results between living and apoptotic cells, illustrating the potential of DNA scaffolds responsive to more complex pH triggers in living systems.
The caption/legend for the online abstract figure: Schematic illustration of cell apoptosis detection in Ramos cells by using duplex-triplex/GO nanocomplex
研究领域:
分子生物学
·
纳米技术
·
生物医学工程
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）