Analytical Methods
·
2014
·Vol. 6
·期 22
·9073-9077
·
IF 1.938
Yu Zhang,Liqian Wu,Yurong Tang,Yingying Su,Yi Lv
A new fluorescent probe for detecting lead ions (Pb2+) is reported in this work. Positively charged UCPs capped with ethylene imine polymer (PEI) and negatively charged AuNPs capped with 11-mercaptoundecanoic acid (MUA) were used as fluorescent donors and acceptors, respectively. The fluorescence quenching process was initiated by the electrostatic interaction between PEI–UCPs and MUA–AuNPs. Upon the addition of Pb2+, functionalized AuNPs were aggregated by an ion-templated chelation process, causing the departure of AuNPs from UCPs. As a result, the fluorescence of the donor was restored and the recovery was found to be proportional within the range of 0.5–10 μM to the concentration of Pb2+. The detection limit was found to be 20 nM. This method was easy to operate with satisfactory selectivity and remarkable sensitivity which would be meaningful in Pb2+-related detection.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 8
·1-8
·
IF 5.3
Guo Keyu,Liu Tong,Li Shun,Zhu Daobin,Fan Yuanyuan,Fan Rangrang,Ding Changchun,Jin Wei,Hu Junshan
An upconversion luminescence (UCL) nanoprobe is reported for the rapid detection of hypochlorite ions (ClO − ) in complex environments. The probe is fabricated by sensitizing rhodamine B (RhB) on the surface of core@shell upconversion nanoparticles (C/S UCNPs) with the bridging of polyethylene glycol (PEG) molecules. Under 980 nm excitation, the UCL of C/S UCNPs is quenched by RhB through fluorescence resonance energy transfer (FRET), which can be restored by the oxidative cleavage of RhB induced by ClO − . It is observed that the green emission intensity of RhB-modified C/S UCNPs increases linearly with the concentration of ClO − , enabling quantitative detection of ClO − . Moreover, the excitation of C/S UCNPs requires only a low power density (0.09 W/cm 2 ), which minimizes the photodegradation of RhB and stabilizes the FRET process. Under optimized conditions, the detection limit for ClO − ions in tap water was 5 µg mL⁻ 1 . Graphical abstract
研究领域:
分析化学
·
纳米材料
·
生物传感
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Xin-Jie Song,Fei Ye,Yao Zhang,Juan Sun,Xuping Shentu,Xiaoping Yu,Wei Li,Yuan-Feng Wu
In this study, a fluorescence detection method combining aptamer-modified up-conversion nanoparticles (UCNPs) and magnetic nanoparticles (MNPs) was developed for detection of Clenbuterol (CLB). The aptamer-modified magnetic NPs captured CLB, which reacted with the aptamer-modified UCNPs and generated a sandwich complex. The aptamer-modified UCNPs acted as a fluorescence source. The MNP-CLB-UCNP complex was retrieved from the solution using an magnetic field, and the fluorescence intensity was detected by fluorescence spectrophotometry with excitation and emission spectra at 980 nm and in the 400–800 nm region, respectively. The results showed that the fluorescence intensity gradually increased with increasing concentrations of CLB with a good specify. The method was highly sensitive for the quantification of CLB, with a limit of detection of 0.304 ng mL −1 . The recovery rate of CLB from pork samples ranged from 84 % to 94.87 %. This fluorescence method enables the sensitive, precise, and accurate quantification of CLB residues in pork samples.
研究领域:
分析化学
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食品安全
·
生物医学
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DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 8
·3391
·
IF 4.6
Qidi Pan,Zhe Gao,He Meng,Xianghua Guo,Meitian Zhang,Yiwei Tang
A novel, molecularly imprinted, upconversion fluorescence probe (UCNP@MIFP) for sulfonamide sensing was fabricated by Pickering emulsion polymerization using UCNP@SiO2particles as the stabilizer and sulfamethazine/sulfamerazine as the co-templates. The synthesis conditions of the UCNP@MIFP were optimized, and the synthesized probe was characterized by scanning electron microscopy, Fourier transform infrared spectrometer, thermogravimetric analyzer, and fluorescence spectrometer. The UCNP@MIFPs showed a good adsorption capacity and a fast kinetic feature for the template. The selectivity experiment revealed that the UCNP@MIFP has a broad-spectrum molecular recognition capability. Good linear relationships were obtained over the concentration range of 1–10 ng/mL for sulfamerazine, sulfamethazine, sulfathiazole, and sulfafurazole, with low limits of detection in the range of 1.37–2.35 ng/mL. The prepared UCNP@MIFP has the potential to detect four sulfonamide residues in food and environmental water.Keywords:molecularly imprinted polymer;Pickering emulsion polymerization;upconversion fluorescence probe;sulfonamides
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Taotao Ren,Yiwei Lu,Peng Liu,Xuelian Hu,Wenxiu Wang,Shuo Wang,Xiuying Liu,Yiwei Tang
Listeria monocytogenes ( L. monocytogenes ) is a foodborne pathogen with high morbidity and mortality rates, necessitating rapid detection methods. Current techniques, while reliable, are labor-intensive and not amenable to on-site testing. We report the design and synthesis of a novel imprinted upconversion fluorescence probe through Pickering emulsion polymerization for the specific detection of L. monocytogenes . The probe employs trimethylolpropane trimethacrylate and divinylbenzene as cross-linkers, acryloyl-modified chitosan as a functional monomer, and the bacterium itself as the template. The developed probe demonstrated high specificity and sensitivity in detecting L. monocytogenes , with a limit of detection of 72 CFU/mL. It effectively identified the pathogen in contaminated salmon and chicken samples, with minimal background interference. The integration of molecular imprinting and upconversion fluorescence materials presents a potent and reliable approach for the rapid and specific detection of L. monocytogenes , offering considerable potential for on-site food safety testing.
研究领域:
食品安全
·
微生物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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